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The transcription factor IbNAC29 positively regulates the carotenoid accumulation in sweet potato

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Horticulture Research 2023年第3期10卷 189-200页

作者： Shihan Xing Ruijie Li Haoqiang Zhao Hong Zhai Shaozhen He Huan Zhang Yuanyuan Zhou Ning Zhao Shaopei Gao Qingchang Liu key laboratory of Sweet Potato Biology and biotechnology Ministry of Agriculture and Rural Affairs/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and UtilizationMinistry of EducationCollege of Agronomy&BiotechnologyChina Agricultural UniversityBeijing 100193China

Carotenoid is a tetraterpene pigment beneficial for human *** the carotenoid biosynthesis pathway has been extensively studied in plants,relatively little is known about their regulation in sweet ***,we conducted the transcriptome database of differentially expressed genes between the sweet potato(Ipomoea batatas)cultivar‘Weiduoli’and its high-carotenoid mutant‘HVB-3’.In this study,we selected one of these candidate genes,IbNAC29,for subsequent ***29 belongs to the plant-specific NAC(NAM,ATAF1/2,and CUC2)transcription factor *** IbNAC29 mRNA level in the HVB-3 storage roots was∼1.71-fold higher than *** experiments showed that the contents ofα-carotene,lutein,β-carotene,zeaxanthin,and capsanthin are obviously increased in the storage roots of transgenic sweet potato plants overexpressing ***,the levels of carotenoid biosynthesis genes in transgenic plants were also ***,yeast one-hybrid assays indicated that IbNAC29 could not directly bind to the promoters of these carotenoid biosynthesis ***,the level of IbSGR1 was down-regulated,whose homologous genes in tomato can negatively regulate carotene *** three-hybrid analysis revealed that the IbNAC29-IbMYB1R1-IbAITR5 could form a regulatory *** one-hybrid,electrophoretic mobility shift assay,quantitative PCR analysis of chromatin immunoprecipitation and dual-luciferase reporter assay showed that IbAITR5 directly binds to and inhibits the promoter activity of IbSGR1,up-regulating carotenoid biosynthesis gene *** together,IbNAC29 is a potential candidate gene for the genetic improvement of nutritive value in sweet potato.

关键词： roots sweet potato

Sob gene is critical to wing development in Bombyx mori and Tribolium castaneum

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Insect Science 2022年第1期29卷 65-77页

作者： Zhan-Feng Ye Pan Zhang Ting-Ting Gai Jing-Hou Lou Fang-Yin Dai Xiao-Ling Tong State key laboratory of Silkworm Genome Biology Key Laboratory of Sericultural Biology and Genetic BreedingMinistry of Agriculture and Rural AffairsCollege of BiotechnologySouthwest UniversityChongqingChina

The development of insect appendages requires the expression of multiple genes in a strict spatial and temporal order. The odd-skipped family genes are vital transcriptional factors involved in embryonic development. The development and morphogenesis of the insect wing requires multiple transcription factors to regulate the expression of wing patterning genes at the transcriptional level. However, the function of odd-related genes in insect wing morphogenesis and development during postembryonic stages is unclear. We focused on the roles of the sister of odd and bowl (sob) gene, a member of odd-skipped family genes, during the wing morphopoiesis in Bombyx mori using the clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein 9 system and in Tribolium castaneum by RNA interference. The results showed that the wings were significantly smaller and degenerated, and wing veins were indistinct in the sob gene loss-of-function group in both B. mori and T. castaneum. Quantitative real-time polymerase chain reaction revealed that the Tcsob gene regulated the expression of wing development genes, such as the cht 7 and the vg gene. The findings suggest the importance of sob gene in insect wing morphology formation during postembryonic stages.

关键词： Bombyx mori sob gene transcription factor Tribolium castaneum wing

Sugarcane transcription factor ScWRKY4 negatively regulates resistance to pathogen infection through the JA signaling pathway

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The crop Journal 2024年第1期12卷 164-176页

作者： Dongjiao Wang Wei Wang Shoujian Zang Liqian Qin Yanlan Liang Peixia Lin Yachun Su Youxiong Que key laboratory of Sugarcane Biology and Genetic breeding Ministry of Agriculture and Rural AffairsKey Laboratory of GeneticsBreeding and Multiple Utilization of CropsMinistry of EducationCollege of AgricultureFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina National key laboratory for Tropical crop breeding Institute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agricultural SciencesSanya 572024HainanChina

WRKY transcription factors,transcriptional regulators unique to plants,play an important role in defense response to pathogen ***,the resistance mechanisms of WRKY genes in sugarcane remain *** the present study,gene ontology(GO)enrichment analysis revealed that WRKY gene family in sugarcane was extensively involved in the response to biotic stress and in defense *** identified gene ScWRKY4,a classⅡc member of the WRKY gene family,in sugarcane cultivar *** gene was induced by salicylic acid(SA)and methyl jasmonate(MeJA)***,expression of ScWRKY4 was down-regulated in smut-resistant sugarcane cultivars but up-regulated in smutsusceptible sugarcane cultivars infected with Sporisorium ***,stable overexpression of the ScWRKY4 gene in Nicotiana benthamiana enhanced susceptibility to Fusarium solani *** and caused down-regulated expression of immune marker-related *** analysis indicated suppressed expression of most JAZ genes in the signal transduction ***4 interacted with ScJAZ13 to repress its *** thus hypothesized that the ScWRKY4 gene was involved in the regulatory network of plant disease resistance,most likely through the JA signaling *** present study depicting the molecular involvement of ScWRKY4 in sugarcane disease resistance lays a foundation for future investigation.

关键词： Disease resistance Expression profile Transcriptome analysis WRKY transcription factors

A geranylgeranyl pyrophosphate synthase gene, Ib GGPS, increases carotenoid contents in transgenic sweetpotato

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Journal of Integrative agriculture 2022年第9期21卷 2538-2546页

作者： LI Rui-jie ZHAI Hong HE Shao-zhen ZHANG Huan ZHAO Ning LIU Qing-chang key laboratory of Sweetpotato Biology and biotechnology Ministry of Agriculture and Rural Affairs/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and UtilizationMinistry of Education/College of Agronomy&BiotechnologyChina Agricultural UniversityBeijing 100193P.R.China

Geranylgeranyl pyrophosphate synthase(GGPS) plays an important role in the biosynthesis of carotenoids. In a previous study, the IbGGPS gene was isolated from a sweetpotato, Ipomoea batatas(L.) Lam., line Nongdafu 14 with high carotenoid contents, but its role and underlying mechanisms in carotenoid biosynthesis in sweetpotato were not investigated. In the present study, the IbGGPS gene was introduced into a sweetpotato cv. Lizixiang and the contents of β-carotene, β-cryptoxanthin, zeaxanthin and lutein were significantly increased in the storage roots of the IbGGPSoverexpressing sweetpotato plants. Further analysis showed that IbGGPS gene overexpression systematically upregulated the genes involved in the glycolytic, 2-C-methyl-D-erythritol-4-phosphate(MEP) and carotenoid pathways,which increased the carotenoid contents in the transgenic plants. These results indicate that the IbGGPS gene has the potential for use in improving the carotenoid contents in sweetpotato and other plants.

关键词： sweetpotato Ipomoea batatas(L.)Lam. IbGGPS overexpression carotenoid

Analysis of genetic diversity and population structure in sweetpotato using SSR markers

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Journal of Integrative agriculture 2023年第11期22卷 3408-3415页

作者： LIU Cheng ZHAO Ning JIANG Zhi-cheng ZHANG Huan ZHAI Hong HE Shao-zhen GAO Shao-pei LIU Qing-chang key laboratory of Sweetpotato Biology and biotechnology Ministry of Agriculture and Rural Affairs/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and UtilizationMinistry of Education/College of Agronomy&BiotechnologyChina Agricultural UniversityBeijing 100193P.R.China

Sweetpotato, Ipomoea batatas(L.) Lam., is an important food crop worldwide. Large scale evaluation of sweetpotato germplasm for genetic diversity is necessary to determine the genetic relationships between them and effectively use them in the genetic improvement. In this study, the genetic diversity of 617 sweetpotato accessions, including 376landraces and 162 bred varieties from China and 79 introduced varieties from 11 other countries, was assessed using 30 simple sequence repeat(SSR) primer pairs with high polymorphism. Based on the population structure analysis,these sweetpotato accessions were divided into three groups, Group 1, Group 2 and Group 3, which included 228, 136and 253 accessions, respectively. Consistent results were obtained by phylogenic analysis and principal coordinate analysis(PCoA). Of the three groups, Group 2 showed the highest level of genetic diversity and its accessions were mainly distributed in low-latitude regions. The accessions from South China exhibited the highest level of genetic diversity, which supports the hypothesis that Fujian and Guangdong were the first regions where sweetpotato was introduced to China. Analysis of molecular variance(AMOVA) indicated significant genetic differentiations between the different groups, but low levels of genetic differentiation existed between the different origins and accession *** results provide valuable information for the better utilization of these accessions in sweetpotato breeding.

关键词： sweetpotato genetic diversity population structure SsR

Development of a high-density SSR genetic linkage map in sweet potato

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The crop Journal 2021年第6期9卷 1367-1374页

作者： Yusha Meng Chenxing Zheng Hui Li Aixian Li Hong Zhai Qingmei Wang Shaozhen He Ning Zhao Huan Zhang Shaopei Gao Qingchang Liu key laboratory of Sweetpotato Biology and biotechnology Ministry of Agriculture and Rural Affairs/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis&Utilization and Joint Laboratory for International Cooperation in Crop Molecular BreedingMinistry of EducationCollege of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China crop Research Institute Shandong Academy of Agricultural SciencesJinan 250100ShandongChina

Simple sequence repeat(SSR) markers have been proved to be a very powerful tool for quantitative trait locus(QTL) mapping, marker-assisted selection and comparative genomics research in many crop species. However, a high-density SSR genetic linkage map is still lacking because there are only a few SSR markers available in sweet potato. In this study, a total of 2545 simple sequence repeat(SSR) primer pairs, including 1215 genomic SSR(gSSR) primer pairs and 1330 BES-SSR(bSSR) primer pairs designed from the genome sequence and BAC-end sequence of sweet potato, respectively, were screened with sweet potato cultivars Luoxushu 8 and Zhengshu 20 and their randomly sampled two F1 individuals and 571 of them generated polymorphic bands. The selected 571 polymorphic SSR primer pairs and 35 EST-based SSR(eSSR) primer pairs developed at our laboratory were used to genotype 240 F1 individuals derived from a cross between Luoxushu 8 and Zhengshu 20. A double pseudo-test-cross strategy was applied for linkage analysis. The Luoxushu 8 map included 90 linkage groups with 5057 SSR markers and covered 13,299.9 cM with a marker density of 2.6 cM, and the Zhengshu 20 map contained 90 linkage groups with 3009 SSR markers and covered 11,122.9 cM with a marker density of 3.7 cM. Fifteen homologous groups were identified in both parent maps. These are the first SSR linkage maps consisting of the complete 90 linkage groups and 15 homologous groups, which are consistent with the autohexaploid nature of sweetpotato, and are also the linkage maps with the highest SSR marker density reported to *** results provide a basis for QTL mapping, marker-assisted breeding and comparative genomics research of sweet potato.

关键词： Sweet potato SSR Pseudo-test-cross strategy Linkage map Autohexaploid

A sucrose non-fermenting-1-related protein kinase-1 gene, IbSnRK1, confers salt, drought and cold tolerance in sweet potato

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The crop Journal 2020年第6期8卷 905-917页

作者： Zhitong Ren Shaozhen He Yuanyuan Zhou Ning Zhao Tao Jiang Hong Zhai Qingchang Liu key laboratory of Sweetpotato Biology and biotechnology Ministry of Agriculture and Rural Affairs/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis&Utilization and Joint Laboratory for International Cooperation in Crop Molecular BreedingMinistry of EducationCollege of Agronomy&BiotechnologyChina Agricultural UniversityBeijing 100193China College of Agronomy Qingdao Agricultural UniversityQingdao 266109ShandongChina

Sucrose non-fermenting-1-related protein kinase-1(SnRK1)regulates carbon and nitrogen metabolism in ***,its roles and their underlying mechanisms in tolerance to abiotic stresses are little *** present study indicated that the IbSnRK1 gene was strongly induced by Na Cl,polyethylene glycol(PEG)6000,hydrogen peroxide(H2O2),cold(4°C),and abscisic acid(ABA).Its overexpression significantly increased salt,drought,and cold tolerance in transgenic sweet potato ***,proline,and K+contents were significantly increased,whereas malondialdehyde(MDA),Na+and H2O2 contents and O2-production rate were significantly decreased in the transgenic plants under salt,drought,and cold *** of the gene up-regulated genes involved in ABA biosynthesis,stress response,and stomatal closure;increased enzyme activities in the reactive oxygen species(ROS)scavenging system;and controlled stomatal closure under salt,drought,and cold *** results show that the IbSnRK1 gene confers salt,drought,and cold tolerance in sweet potato by activating the ROS scavenging system and controlling stomatal closure via the ABA signaling pathway.

关键词： Sweet potato IbSnRK1 Abiotic stress tolerance ABA ROS

Hepatic steatosis is associated with dysregulated cholesterol metabolism and altered protein acetylation dynamics in chickens

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Journal of Animal Science and biotechnology 2024年第1期15卷 53-67页

作者： Xiaoli Guo Qianqian Zhou Jiaming Jin Fangren Lan Chaoliang Wen Junying Li Ning Yang Congjiao Sun National Engineering laboratory for Animal breeding and key laboratory of Animal Genetics Breeding and ReproductionMinistry of Agriculture and Rural AffairsChina Agricultural UniversityBeijing 100193China

Background Hepatic steatosis is a prevalent manifestation of fatty liver, that has detrimental effect on the health and productivity of laying hens, resulting in economic losses to the poultry industry. Here, we aimed to systematically investigate the genetic regulatory mechanisms of hepatic steatosis in laying *** Ninety individuals with the most prominent characteristics were selected from 686 laying hens according to the accumulation of lipid droplets in the liver, and were graded into three groups, including the control, mild hepatic steatosis and severe hepatic steatosis groups. A combination of transcriptome, proteome, acetylome and lipidome analyses, along with bioinformatics analysis were used to screen the key biological processes, modifications and lipids associated with hepatic *** The rationality of the hepatic steatosis grouping was verified through liver biochemical assays and RNA-seq. Hepatic steatosis was characterized by increased lipid deposition and multiple metabolic abnormalities. Integration of proteome and acetylome revealed that differentially expressed proteins(DEPs) interacted with differentially acetylated proteins(DAPs) and were involved in maintaining the metabolic balance in the liver. Acetylation alterations mainly occurred in the progression from mild to severe hepatic steatosis, i.e., the enzymes in the fatty acid oxidation and bile acid synthesis pathways were significantly less acetylated in severe hepatic steatosis group than that in mild group(P < 0.05). Lipidomics detected a variety of sphingolipids(SPs) and glycerophospholipids(GPs) were negatively correlated with hepatic steatosis(r ≤-0.5, P < 0.05). Furthermore, the severity of hepatic steatosis was associated with a decrease in cholesterol and bile acid synthesis and an increase in exogenous cholesterol *** In addition to acquiring a global and thorough picture of hepatic steatosis in laying hens, we were able to reveal the role of ac

关键词： Acetylation Cholesterol metabolism Hepatic steatosis Laying hens Multiomics

Genetic basis of negative heterosis for growth traits in chickens revealed by genome-wide gene expression pattern analysis

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Journal of Animal Science and biotechnology 2021年第4期12卷 1267-1280页

作者： Chunning Mai Chaoliang Wen Zhiyuan Xu Guiyun Xu Sirui Chen Jiangxia Zheng Congjiao Sun Ning Yang National Engineering laboratory for Animal breeding and key laboratory of Animal Genetics Breeding and ReproductionMinistry of Agriculture and Rural AffairsChina Agricultural UniversityBeijing 100193China

Background:Heterosis is an important biological phenomenon that has been extensively utilized in agricultural ***,negative heterosis is also pervasively observed in nature,which can cause unfavorable impacts on production *** with systematic studies of positive heterosis,the phenomenon of negative heterosis has been largely ignored in genetic studies and breeding programs,and the genetic mechanism of this phenomenon has not been thoroughly elucidated to ***,we used chickens,the most common agricultural animals worldwide,to determine the genetic and molecular mechanisms of negative ***:We performed reciprocal crossing experiments with two distinct chicken lines and found that the body weight presented widely negative heterosis in the early growth of *** heterosis of carcass traits was more common than positive heterosis,especially breast muscle mass,which was over−40%in reciprocal ***-wide gene expression pattern analyses of breast muscle tissues revealed that nonadditivity,including dominance and overdominace,was the major gene inheritance *** genes,including a substantial number of genes encoding ATPase and NADH dehydrogenase,accounted for more than 68%of differentially expressed genes in reciprocal crosses(4257 of 5587 and 3617 of 5243,respectively).Moreover,nonadditive genes were significantly associated with the biological process of oxidative phosphorylation,which is the major metabolic pathway for energy release and animal growth and *** detection of ATP content and ATPase activity for purebred and crossbred progenies further confirmed that chickens with lower muscle yield had lower ATP concentrations but higher hydrolysis activity,which supported the important role of oxidative phosphorylation in negative heterosis for growth traits in ***:These findings revealed that nonadditive genes and their related oxidative phosphorylation were the m

关键词： Chicken Growth Gene expression patterns Heterosis Oxidative phosphorylation