Sob gene is critical to wing development in Bombyx mori and Tribolium castaneum
作者机构：State Key Laboratory of Silkworm Genome BiologyKey Laboratory of Sericultural Biology and Genetic BreedingMinistry of Agriculture and Rural AffairsCollege of BiotechnologySouthwest UniversityChongqingChina
出 版 物：《Insect Science》 (昆虫科学（英文版）)
年 卷 期：2022年第29卷第1期
页 面：65-77页
核心收录：
学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 09[农学] 0904[农学-植物保护] 0901[农学-作物学] 0713[理学-生态学]
基 金：This project received funding from the National Natural Science Foundation of China(No.U20A2058,No.31830094,No.31472153) Funds of China Agriculture Research System(No.CARS-18-ZJO102)
主 题：Bombyx mori sob gene transcription factor Tribolium castaneum wing
摘 要：The development of insect appendages requires the expression of multiple genes in a strict spatial and temporal order. The odd-skipped family genes are vital transcriptional factors involved in embryonic development. The development and morphogenesis of the insect wing requires multiple transcription factors to regulate the expression of wing patterning genes at the transcriptional level. However, the function of odd-related genes in insect wing morphogenesis and development during postembryonic stages is unclear. We focused on the roles of the sister of odd and bowl (sob) gene, a member of odd-skipped family genes, during the wing morphopoiesis in Bombyx mori using the clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein 9 system and in Tribolium castaneum by RNA interference. The results showed that the wings were significantly smaller and degenerated, and wing veins were indistinct in the sob gene loss-of-function group in both B. mori and T. castaneum. Quantitative real-time polymerase chain reaction revealed that the Tcsob gene regulated the expression of wing development genes, such as the cht 7 and the vg gene. The findings suggest the importance of sob gene in insect wing morphology formation during postembryonic stages.
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