检索结果-南通市图书馆

Carrimycin inhibits coronavirus replication by decreasing the efficiency of programmed–1 ribosomal frameshifting through directly binding to the RNA pseudoknot of viral frameshift-stimulatory element

引用

Acta Pharmaceutica Sinica B 2024年第6期14卷 2567-2580页

作者： Hongying Li Jianrui Li Jiayu Li Hu Li Xuekai Wang Jing Jiang Lei Lei Han Sun Mei Tang Biao Dong Weiqing He Shuyi Si Bin Hong Yinghong Li Danqing Song Zonggen Peng Yongsheng Che Jian-Dong Jiang CAMS key laboratory of Antiviral Drug Research Institute of Medicinal BiotechnologyChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100050China Beijing key laboratory of Antimicrobial Agents Institute of Medicinal BiotechnologyChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100050China key laboratory of Biotechnology of Antibiotics the National Health and Family Planning Commission(NHFPC)Institute of Medicinal BiotechnologyChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100050China

The pandemic of SARS-CoV-2 worldwide with successive emerging variants urgently calls for small-molecule oral drugs with broad-spectrum antiviral ***,we show that carrimycin,a new macrolide antibiotic in the clinic and an antiviral candidate for SARS-CoV-2 in phase III trials,decreases the efficiency of programmed–1 ribosomal frameshifting of coronaviruses and thus impedes viral replication in a broad-spectrum *** binds directly to the coronaviral frameshift-stimulatory element(FSE)RNA pseudoknot,interrupting the viral protein translation switch from ORF1a to ORF1b and thereby reducing the level of the core components of the viral replication and transcription *** carrimycin with known viral replicase inhibitors yielded a synergistic inhibitory effect on *** the FSE mechanism is essential in all coronaviruses,carrimycin could be a new broad-spectrum antiviral drug for human coronaviruses by directly targeting the conserved coronaviral FSE *** finding may open a new direction in antiviral drug discovery for coronavirus variants.

关键词： Carrimycin Coronavirus Broad-spectrum antiviral activity Programmed-1 ribosomal frameshifting RNA pseudoknot Antiviral agent RNA target Synergistic inhibitory effect

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

PACS gene family-related neurological diseases: limited genotypes and diverse phenotypes

引用

World Journal of Pediatrics 2024年第1期20卷 82-91页

作者： Han Zhang Kai Gao Shuang Wang Yue-Hua Zhang Zhi-Xian Yang Ye Wu Yu-Wu Jiang Department of Pediatrics Peking University First Hospital.No.1 Xi'an Men StreetWest DistrictBeijing 100034China Bejing key laboratory of Molecular Diagnosis and Study on Pediatric Genetic Diseases BeijingChina Children Epilepsy Center Peking University First HospitalBeijingChina key laboratory for Neuroscience Ministry of Education/National Health and Family Planning CommissionPeking UniversityBeijingChina Center of Epilepsy Beijing Institute for Brain DisordersBeijingChina

Background The PACS gene family has been demonstrated to be related to intracellular vesicular *** phenotypic manifestations caused by the pathogenic variants of PACS include epilepsy,intellectual disability/developmental delay,and malformations,such as facial *** We identified seven new cases with pathogenic or likely pathogenic PACS variants using next-generation *** information obtained from these patients was analyzed along with that obtained from previously reported *** With the inclusion of the newly diagnosed cases in this study,103 cases with PACS gene family-related neurological diseases were reported,of which 43 were PACS2-related cases and the remaining were PACSI-related *** patients had seizures,which have been reported to be effectively controlled by several types of anti-seizure medications(ASMs).The most efficacious and frequently prescribed ASMs included sodium valproate(43.3%,13/30),oxcarbazepine/carbamazepine(26.7%,8/30),and levetiracetam(20%,6/30).Almost all patients had intellectual disability/developmental *** most common pathogenic missense variants were PACSI ***203Trp and PACS2 *** addition,we report a patient carrying a likely pathogenic copy number variation(CNV)(de novo heterozygous deletion of chr14:105821380-106107443,286 kilobase,destroyed part of the furin-binding region domain and the protein structure after it)with more severe and refractory late-onset *** The clinical phenotypes of the different PACS heterozygous missense variants were *** pathogenic variant sites of PACSI and PACS2 were quite limited but located in different regions.A CNV destroying part of the PACS2 gene might also be *** findings may provide an important clue for further functional studies on the pathogenic mechanism of neurological disorders related to the PACS gene family.

关键词： Developmental delay Epilepsy Intellectual disability PACSI PACS2

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

PENG ZU Study on healthy Aging in China(PENG ZU Cohort):Design and Goals

引用

China CDC weekly 2024年第34期6卷 876-882页

作者： Ju Cui Jing Pang Liqun Zhang Juan Li Xiaolan Wu Xinyi Zhu Wei Ma Senlin Luo Huafang Gao Zhao Wang Jianping Cai Tiemei Zhang The key laboratory of Geriatrics Beijing Institute of GeriatricsInstitute of Geriatric MedicineChinese Academy of Medical ScienceBeijing Hospital/National Center of Gerontology of National Health CommissionBeijingChina Beijing Institute of Geriatrics Beijing HospitalBeijingChina Institute of Geriatric Medicine Chinese Academy of Medical ScienceBeijing HospitalBeijingChina Center on Aging Psychology CAS Key Laboratory of Mental HealthInstitute of PsychologyChinese Academy of SciencesBeijingChina Institute of healthy Ageing China Research Center on AgeingBeijingChina Institute of Statistics and Big Data Renmin University of ChinaBeijingChina Beijing Institute of Technology BeijingChina national Human Genetic Resources Center National Research Institute for Family PlanningBeijingChina Department of Pharmacology School of Pharmaceutical SciencesTsinghua UniversityBeijingChina

Life expectancy is increasing,leading to the continuous aging of the population in *** the health status of the older population is crucial to achieving healthy *** primary objective of the PENG ZU Study on healthy Aging in China(PENG ZU Cohort)is to understand the natural progression of health status among the aging Chinese ***,the PENG ZU cohort aims to identify and validate multidimensional aging markers,uncover the underlying mechanisms of systemic aging and functional decline,and develop novel strategies and measures to delay functional decline and adverse health outcomes,while maintaining overall good *** PENG ZU cohort consists of 26,000 individuals aged 25 to 89 years from seven major geographical regions in *** data and biospecimens are collected according to standardized procedures at baseline and follow-up *** recruitment for the PENG ZU cohort was completed in October *** extensive analysis of multidimensional health-related data and bioresources collected from the cohort is anticipated to develop methods for evaluating functional status and elucidating multilevel,cross-scale interactions and regulatory mechanisms of healthy *** findings from this study will enhance the understanding of health changes due to aging,facilitate efficient and effective interventions to maintain functional ability,and reduce the incidence and severity of age-related diseases,thereby further promoting healthy aging.

关键词： aging thereby maintain

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The influence of corneal ablation patterns on prediction error after cataract surgery in post-myopic-LASIK eyes

引用

Eye and Vision 2024年第4期10卷 23-31页

作者： Yunqian Yao jing Zhao Jifeng Yu Wenwen He Ling Wei Xingtao Zhou Yi Lu Xiangjia Zhu Eye Institute and Department of Ophthalmology Eye&ENT HospitalFudan University83 Fenyang RoadShanghai 200031China national health Center key laboratory of Myopia(Fudan University) Key Laboratory of MyopiaChinese Academy of Medical SciencesShanghaiChina Shanghai key laboratory of Visual Impairment and Restoration ShanghaiChina Shanghai Research Center of Ophthalmology and Optometry ShanghaiChina Department of Ophthalmology Beijing Children's HospitalNational Center for Children's HealthCapital Medical UniversityBeijingChina State key laboratory of Medical Neurobiology Fudan UniversityShanghaiChina

Purpose:To evaluate the influence of corneal ablation patterns on the prediction error after cataract surgery in postmyopic-LASIK ***:Eighty-three post-myopic-LASIK eyes of 83 patients that underwent uneventful cataract surgery were retrospectively *** postoperative spherical equivalence(SE)was calculated for the implanted lens using the Haigis-L and Barrett True-K *** error at one month postsurgery was calculated as actual SE minus predicted *** each eye,area and decentration of the ablation zone was measured using the tangential curvature *** associations between prediction errors and corneal ablation patterns were ***:The mean prediction error was-0.83±1.00 D with the Haigis-L formula and-1.00±0.99 D with the Barrett True-K *** error was positively correlated with keratometry(K)value and negatively correlated with ablation zone area using either formula,and negatively correlated with decentration of the ablation zone using the Barrett True-K formula(all P<0.05).In the K<37.08 D group,prediction error was negatively correlated with decentration of the ablation zone with both formulas(all P<0.05).Multivariate analysis showed that with the Haigis-L formula,prediction error was associated with axial length(AL),K value and decentration,and with the Barrett True-K formula,prediction error was associated with AL and decentration(all P<0.05).Conclusion:A flatter cornea,larger corneal ablation zone and greater decentration will lead to more myopic prediction error after cataract surgery in post-myopic-LASIK eyes.

关键词： Cataract surgery Laser in situ keratomileusis Intraocular lens power Keratometry Ablation zone decentration Ablation zone size

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cobalamin-dependent radical S-adenosyl-L-methionine protein functions with a partner to successively methylate tricyclic indole alkaloid for chuangxinmycin maturation and derivatization

引用

Science China Chemistry 2024年

作者： Sili Wang Jiancheng Huang Yanan Du Wei Huang Yufeng Xue Xiaokun Xu Guannan Zhong Yuanyuan Shi Bin Hong Xiaoying Bian Wen Liu State key laboratory of Chemical Biology Shanghai Institute of Organic ChemistryUniversity of Chinese Academy of SciencesChinese Academy of Sciences State key laboratory of Microbial Technology Shandong University national health commission (NHC) key laboratory of Biotechnology for Microbial Drugs Institute of Medicinal BiotechnologyChinese Academy of Medical Science&Peking Union Medical College

Cobalamin (Cbl)-dependent radical S-adenosyl-L-methionine (SAM) proteins constitute the largest collection of the radical SAM superfamily that has hundreds of thousands of individual *** of these proteins are involved in the biosynthesis of pharmaceutically important natural products to catalyze chemically demanding *** the biosynthetic pathway of chuangxinmycin (CXM),a unique indole alkaloid antibiotic with potent anti-infective activity,functionalization of the characteristic thiopyrano[4,3,2-cd]indole scaffold by regio-and stereoselective C3-methylation is believed to rely on a Cbl-dependent radical process,which,however,remained to be reconstituted *** here report the dissection of this enzymatic process,which requires the incorporation of Cxm8,a Cbl-dependent radical SAM protein,with Cxm9,a DUF5825family protein that shares no homology to any proteins of known ***8 and Cxm9 function together by forming an unexpected heterodimeric complex that selectively catalyzes C3-methylation of the tricyclic indole-S-hetero ring system in a successive manner,achieving CXM and a recently identified,C3-dimethylated *** biochemical characterization,isotope labeling,structural simulation and bioinformatics analysis rationalized the catalysis of the Cxm8/Cxm9 complex and particularly the necessity of the DUF5825 protein for C3-methylase *** is the first example that a Cbl-dependent protein acts with a partner to exhibit radical SAM activity.

关键词：

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Prenatal Diagnosis of Fukuyama Congenital Muscular Dystrophy by Optical Genomic Mapping in a Chinese family

引用

Maternal-Fetal Medicine 2024年第3期6卷 131-133页

作者： Jia Zhou Hui Xiong Jingmin Yang Xiaona Fu Luming Sun Department of FetalMedicine Shanghai FirstMaternity and Infant HospitalTongji UniversityShanghai201204China Department of Neurology Beijing Children’s HospitalCapital Medical UniversityNational Center for Children’s HealthBeijing 100045China key laboratory of Birth Defects and Reproductive health of national health and family planning commission(Chongqing key laboratory of Birth Defects and Reproductive health Chongqing Population and Family PlanningScience and Technology Research Institute)Chongqing 400020China State key laboratory of Genetic Engineering and MOE Engineering Research Center of Gene Technology School of Life SciencesFudan University200433ShanghaiChina Shanghai Wehealth Biomedical Technology Co. LtdShanghai 201318China

Fukuyama congenital muscular dystrophy(FCMD)is an autosomal recessive disorder first described by Fukuyama in 1960.1 It typically manifests with generalized muscle weakness and hypotonia from infancy,often requiring assistive devices for *** frequently exhibit cognitive deficits,varying degrees of seizures,and specific neuroimaging,such as micropolygyria,hydrocephalus,and cerebellar malformations.

关键词： Mapping muscular dystrophy

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Biocatalytic access to diverse prenylflavonoids by combining a regiospecific C-prenyltransferase and a stereospecific chalcone isomerase

引用

Acta Pharmaceutica Sinica B 2018年第4期8卷 678-686页

作者： Jianhua Li Ridao Chen Ruishan Wang Xiao Liu Kebo Xie Dawei Chen Jungui Dai State key laboratory of Bioactive Substance and Function of natural Medicines Institute of Materia MedicaPeking Union Medical College and Chinese Academy of Medical Sciences key laboratory of biosynthesis of natural products of national health and family planning commission Institute of Materia MedicaPeking Union Medical College and Chinese Academy of Medical Sciences

Prenylflavonoids are valuable natural products that have diverse biological properties, and are usually generated biologically by multiple metabolic enzymes in nature. In this study, structurally diverse prenylflavonoids were conveniently synthesized by enzymatic catalysis by combining GuILDT, a regiospecific chalcone prenyltransferase, and Gu CHI, a stereospecific chalcone isomerase that has promiscuous activity for both chalcones and prenylchalcones as substrates. Our findings provided a new approach for the synthesis of natural/unnatural bioactive prenylflavonoids, including prenylchalcones and optical prenylflavanones with chalcone origins.

关键词： Biocatalysis Prenyltransferase Chalcone isomerase Licorice Prenylflavonoids

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Plant-derived nanovesicles as an emerging platform for cancer therapy

引用

Acta Pharmaceutica Sinica B 2024年第1期14卷 133-154页

作者： Hanzhe Liu Guo-Feng Luo Zhengjun Shang The State key laboratory Breeding Base of Basic Science of Stomatology(Hubei-MOST)&key laboratory of Oral Biomedicine Ministry of Education School and Hospital of StomatologyWuhan UniversityWuhan 430079China

Plant-derived nanovesicles(PDNVs)derived from natural green products have emerged as an attractive nanoplatform in biomedical *** are usually characterized by unique structural and biological functions,such as the bioactive lipids/proteins/nucleic acids as therapeutics and targeting groups,immune-modulation,and long-term *** the rapid development of nanotechnology,materials,and synthetic chemistry,PDNVs can be engineered with multiple functions for efficient drug delivery and specific killing of diseased cells,which represent an innovative biomaterial with high biocompatibility for fighting against *** this review,we provide an overview of the state-of-theart studies concerning the development of PDNVs for cancer *** original sources,methods for obtaining PDNVs,composition and structure are introduced *** an emphasis on the featured application,the inherent anticancer properties of PDNVs as well as the strategies in constructing multifunctional PDNVs-based nanomaterials will be discussed in ***,some scientific issues and technical challenges of PDNVs as promising options in improving anticancer therapy will be discussed,which are expected to promote the further development of PDNVs in clinical translation.

关键词： naturally occurring nanovesicles Plant Nanotechnology Drug delivery Cancer therapy

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Bistachybotrysins D and E,one stereoisomeric pair of cytotoxic phenylspirodrimane dimers from Stachybotrys chartarum

引用

Chinese Chemical Letters 2019年第2期30卷 435-438页

作者： Min Zhang Jiamin Feng Xiaona Jia Jinlian Zhao Jimei Liu Ridao Chen Kebo Xie Dawei Chen Yan Li Dan Zhang Jungui Dai State key laboratory of Bioactive Substance and Function of natural Medicines Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College key laboratory of biosynthesis of natural products of national health and family planning commission Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College

Bistachybotrysins D and E (1 and 2), one stereoisomeric pair of phenylspirodrimane dimers, were isolated from Stachybotrys chartarum CGMCC 3.5365. They represent novel phenylspirodrimane dimers with a central [6,5,6]-tricyclic carbon scaffold containing a cyclopentanone core. The structures of 1 and 2 were elucidated through extensive spectroscopic data analysis, and their absolute configurations were characterized by calculated electronic circular dichroism (ECD). Compounds 1 and 2 displayed potent cytotoxic activity against the four human tumor cell lines HCT116, BGC823, Daoy and HepG2 with IC_ (50)values ranging from 6.7 μmol/L to 11.6 μmol/L. Furthermore, a plausible biogenetic pathway for 1 and 2 is proposed.

关键词： Bistachybotrysin Stereoisomeric pair Phenylspirodrimane dimer Cytotoxic activity Stachybotrys chartarum

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Disinfection Byproducts and Their Precursors in Drinking Water Sources:Origins,Influencing Factors,and Environmental Insights

引用

Engineering 2024年第5期36卷 36-50页

作者： Rong Xiao Yang Deng Zuxin Xu Wenhai Chu State key laboratory of Pollution Control and Resource Reuse College of Environmental Science and EngineeringTongji UniversityShanghai 200092China Ministry of Education key laboratory of the Yangtze River Water Environment Tongji UniversityShanghai 200092China Department of Earth and Environmental Studies Montclair State UniversityMontclairNJ 07043USA

Tracing the contamination origins in water sources and identifying the impacts of natural and human processes are essential for ecological safety and public ***,current analysis approaches are not ideal,as they tend to be laborious,time-consuming,or technically *** byproducts(DBPs)are a family of well-known secondary pollutants formed by the reactions of chemical disinfectants with DBP precursors during water disinfection *** DBP precursors have various origins(e.g.,natural,domestic,industrial,and agricultural sources),and since the formation of DBPs from different precursors in the presence of specific disinfectants is distinctive,we argue that DBPs and DBP precursors can serve as alternative indicators to assess the contamination in water sources and identify pollution *** providing a retrospective of the origins of DBPs and DBP precursors,as well as the specific formation patterns of DBPs from different precursors,this article presents an overview of the impacts of various natural and anthropogenic factors on DBPs and DBP precursors in drinking water *** practice,the DBPs(i.e.,their concentration and speciation)originally present in source water and the DBP precursors determined using DBP formation potential tests—in which water samples are dosed with a stoichiometric excess of specific disinfectants in order to maximize DBP formation under certain reaction conditions—can be considered as alternative *** jointly used with other water quality parameters(e.g.,dissolved organic carbon,dissolved organic nitrogen,fluorescence,and molecular weight distribution)and specific contaminants of emerging concern(e.g.,certain pharmaceuticals and personal care products),DBPs and DBP precursors in drinking water sources can provide a more comprehensive picture of water pollution for better managing water resources and ensuring human health.

关键词： Disinfection byproducts Disinfection byproduct precursors Drinking water sources Contamination indicator natural factors Human factors

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：