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animal nutrition 2021年第3期7卷 801-811页

作者： Saad Gilani Peter V.Chrystal Reza Barekatain Danisco animal nutrition(IFF) Oegstgeestthe Netherlands Baiada Poultry Pty Ltd Pendle HillNSWAustralia South Australian Research and Development Institute Roseworthy CampusRoseworthySAAustralia School of animal and Veterinary Sciences Roseworthy CampusUniversity of AdelaideRoseworthySAAustralia

Maintaining and optimising the intestinal barrier(IB)function in poultry has important implications for the health and performance of the *** a key aspect of the IB,intestinal permeability(IP)is mainly controlled by complex junctional proteins called tight junction proteins(TJ)that link enterocytes *** disruption of TJ is associated with increased gut leakage with possible subsequent impli-cations for bacterial translocation,intestinal inflammation,compromised health and performance of the *** considerable data being available for other species,research on IP in broiler chickens and in general avian species is still an understudied *** paper reviews the available literature with a specific focus on IP in broiler chickens with consideration given to practical factors affecting the IP,current assessment methods,markers and nutritional modulation of *** experimental models to induce gut leakage are discussed including pathogens,rye-based diets,feed deprivation and stress-inducing agents such as exogenous glucocorticoids and heat *** various markers including fluorescein isothiocyanate dextran,expression of TJ and bacterial translocation have been widely utilized to study IP,recent studies have identified a number of excreta biomarkers to evaluate intestinal integrity,in particular non-invasive *** the research on various nutrients and feed additives to potentially modulate IP is still at an early stage,the most promising outcomes are anticipated for probiotics,prebiotics,amino acids and those feed ingredients,nutrients and additives with anti-inflammatory *** research gaps are identified for the mechanistic mode of action of various nutrients to influence IP under different experimental *** modulation of IP through various strategies(*** manipulation of diet)may be regarded as a new frontier for disease prevention and improving the health and performance of poultry particul

关键词： Intestinal barrier function Permeability Tight junction protein Gut leakage model

Seasonal variations in the composition and functional profiles of gut microbiota reflect dietary changes in plateau pikas

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Integrative Zoology 2022年第3期17卷 379-395页

作者： Chao FAN Liangzhi ZHANG Shangang JIA Xianjiang TANG Haibo FU Wenjing LI Chuanfa LIU He ZHANG Qi CHENG Yanming ZHANG key laboratory of Adaptation and Evolution of Plateau Biota Northwest Institute of Plateau BiologyChinese Academy of SciencesXiningChina College of Life Sciences Qufu Normal UniversityQufuChina Qinghai Provincial key laboratory of animal Ecological Genomics XiningChina University of Chinese Academy of Sciences BeijingChina College of Grassland Science and Technology China Agricultural UniversityBeijingChina

Seasonal variations in gut microbiota of small mammals and how they are influenced by environmental variables are relatively poorly *** sampled 162 wild plateau pikas(Ochotona curzoniae)in 4 seasons over 2 and a half years and recorded the air temperature,precipitation,and nutrient content in edible vegetation at the sampling *** conducting 16S rRNA and shotgun metagenomic sequencing,we found that the highest alpha diversity,the relative abundance of Firmicutes,and the simplest co-occurrence network occurred in winter,whereas the highest relative abundance of Proteobacteria and the most complex network structure were observed in *** highest relative abundance of Verrucomicrobiota and Spirochaetota was seen in summer and autumn,*** temperature,precipitation,and the contents of crude protein,crude fiber,and polysaccharide in vegetation had significant effects on the seasonal changes in gut *** contributed more to microbial variation than climatic *** analysis revealed that the amino acid metabolism pathway and axillary activity enzymes were most abundant in summer,while abundance of carbohydrate-binding modules and carbohydrate esterases was highest in *** microbial variations were related to the changes in dietary nutrition,indicating that gut microbiota of plateau pika contribute to the efficient use of food *** study provides new evidence of how external environmental factors affect the intestinal environment of small mammals.

关键词： 16S rRNA gene gut microbiota plateau pikas(Ochotona curzoniae) seasonal variation shotgun metagenomic sequencing

Clostridium butyricum alleviates intestinal low-grade inflammation in TNBS-induced irritable bowel syndrome in mice by regulating functional status of lamina propria dendritic cells

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World Journal of Gastroenterology 2019年第36期25卷 5469-5482页

作者： Qin Zhao Wen-Rong Yang Xiao-Hong Wang Gai-Qin Li Lei-Qi Xu Xiao Cui Yang Liu Xiu-Li Zuo Department of Gastroenterology Qilu Hospital Shandong University Jinan 250012 Shandong Province China Department of Gastroenterology Taian City Central Hospital Taian 271000 Shandong Province China Department of Clinical nutrition Taian City Central Hospital Taian 271000 Shandong Province China Department of Medicine Beijing 316 Hospital Beijing 100093 China

BACKGROUND Irritable bowel syndrome (IBS) is one of the most common functional gastroenterological diseases characterized by abnormal visceral sensitivity and lowgrade inflammation. The role of Clostridium butyricum (C. butyricum) in reducing intestinal low-grade inflammation via immune pathways has been well defined. However, the detailed mechanisms of the effects of C. butyricum on intestinal mucosal immunity, especially on immune cells of the lamina propria, remain unclear. Dendritic cells (DCs), which are important immune cells, secrete proinflammatory cytokines (IL-1β, IL-6, and others) and express T cell immunoglobulin and mucin domain-3 (TIM3), promoting proliferation and activation of DCs, and mediating Th1 and Th17 inflammatory responses. AIM To investigate the role of DCs in the development of IBS in a rat model and to understand the regulation of DCs after C. butyricum intervention. METHODS An IBS animal model was established using C57BL/6 mice, and C. butyricum was continuously administered via the intragastric route to simulate different intestinal immune states. Intestinal visceral hypersensitivity and histopathology were assessed using the abdominal withdrawal reflex (AWR) test and hematoxylin & eosin (H&E) staining, respectively. The expression of proinflammatory cytokines (IL-1β and IL-6) and TIM3 was analyzed by Western blot analysis and real-time PCR. Flow cytometry was applied to analyze the quantity, function, and membrane molecule TIM3 of the lamina propria dendritic cells (LPDCs). The regulatory effect of C. butyricum was verified in bone marrowderived dendritic cells by in vitro experiments. RESULTS The secretion of proinflammatory cytokines (IL-1β and IL-6) in mice with IBS was significantly increased compared with that of the control group, which suggested that the intestinal mucosa in mice with IBS was in a low-grade inflammatory state. The expression of CD11C+CD80+ and CD11c+TIM3+ in intestinal LPDCs in mice with IBS increased significant

关键词： Clostridium butyricum Irritable bowel syndrome Lamina propria dendritic cells T cell immunoglobulin and mucin domain-3 Proinflammatory cytokines

Lactobacillus rhamnosus GG ameliorates DON-induced intestinal damage depending on the enrichment of beneficial bacteria in weaned piglets

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Journal of animal Science and Biotechnology 2022年第6期13卷 1840-1856页

作者： Yongsong Bai Kaidi Ma Jibo Li Zhongshuai Ren Jing Zhang Anshan Shan Institute of animal nutrition Northeast Agricultural UniversityHarbin 150030P.R.China College of animal Sciences Jilin UniversityKey Laboratory of Zoonosis ResearchMinistry of EducationChangchun 130062P.R.China

Background: Deoxynivalenol(DON) is one of the most common environmental pollutants that induces intestinal inflammation and microbiota dysbiosis. Lactobacillus rhamnosus GG(LGG) is a probiotic that not only has anti-inflammatory effects, but also shows protective effect on the intestinal barrier. However, it is still unknown whether LGG exerts beneficial effects against DON-induced intestinal damage in piglets. In this work, a total of 36 weaned piglets were randomized to one of four treatment groups for 21 d. The treatment groups were CON(basal diet);LGG(basal diet supplemented with 1.77 × 10^(11)CFU/kg LGG);DON(DON-contaminated diet) and LGG + DON(DON-contaminated diet supplemented with 1.77 × 10^(11)CFU/kg LGG).Result: Supplementation of LGG can enhance growth performance of piglets exposed to DON by improving intestinal barrier function. LGG has a mitigating effect on intestinal inflammation induced by DON exposure, largely through repression of the TLR4/NF-κB signaling pathway. Furthermore, supplementation of LGG increased the relative abundances of beneficial bacteria(e.g., Collinsella, Lactobacillus, Ruminococcus_torques_group and Anaerofustis), and decreased the relative abundances of harmful bacteria(e.g., Parabacteroides and Ruminiclostridium_6), and also promoted the production of ***: LGG ameliorates DON-induced intestinal damage, which may provide theoretical support for the application of LGG to alleviate the adverse effects induced by DON exposure.

关键词： Deoxynivalenol Gut microbiota Intestinal infammation Lactobacillus rhamnosus GG Piglets

Bacillus licheniformis normalize the ileum microbiota of chickens infected with necrotic enteritis

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Bacillus licheniformis normalize the ileum microbiota of chi...

Establishment of Indirect ELISA Diagnosis Technique based on the VP1 Protein of Foot and Mouth Disease Virus Serotype A

中国畜牧兽医学会动物微生态学分会第五届第十三次全国学术研讨会

作者： Shuai Xu Yicen Lin Dong Zeng Mengjia Zhou Yan Zeng Hesong Wang Yi Zhou Hui Zhu Kangcheng Pan Bo Jing Xueqin Ni animal Microecology Institute College of Veterinary MedicineSichuan Agricultural University key laboratory of animal Disease and Human Health of Sichuan province Sichuan Agricultural University

Necrotic enteritis(NE) is a severe intestinal disease,which can change gut microbiota and result in a high cost for the poultry industry ***,little is known regarding how the gut microbiota of NE chicken ileum are changed by Bacillus *** study was conducted to investigate how ileum microbiota structure was changed by *** in broiler chickens challenged with Clostridium perfringens-induced NE through Illumina MiSeq *** broilers were randomly separated into four groups:the negative control group(NC),the positive control group(PC),the fishmeal and coccidia group(FC),and the PC group supplied with feed containing ***(BL).Compared to the PC and FC,alpha diversity,beta diversity,and the bacterial taxa of the ileum microbiota were more similar in BL and *** genera,which were related to the NE control,became insignificant in BL with NC,such as Lactobacillus,Lactococcus,Bacteroides,Ruminococcus and *** PICRUSt analysis revealed that a tumour suppressor gene,p53,which was negatively correlated with Helicobacter,was enriched in the BL *** findings showed that the ileum microbiota disorder caused by NE in chickens was normalized by dietary *** supplementation.

关键词： Bacillus licheniformis Broiler chickens Necrotic enteritis Illumina MiSeq sequencing Ileum Microbiota

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animal Husbandry and feed Science 2014年第6期6卷 300-303,311页

作者： Lu Qingxia Liu Chang Jin Qianyue Guo Guanpeng Xing Guangxu Liu Yunchao Deng Ruiguang Zhang Gaiping key laboratory of animal Immunology of the Ministry of Agriculture/Henan Provincial key laboratory of animal Immunology Henan Academy of Agricultural Sciences College of Veterinary Medicine and animal Science Henan Agricultural University

The VP1 protein of foot-and-mouth disease virus serotype A was prokaryotically expressed and purified to replace the traditional virus antigen for estab- lishing a fast, safe, effective indirect ELISA method, so as to detecting antibody of foot-and-mouth disease virus serotype A. Western-Blot test showed that the VP1 recombinant protein could be used as detective antigen as it can be specifically recognized by bovine positive serum of FMDV serotype A. By employing matrix titra- tion method, the optimal parameters were obtained as follows： 1 mg/L VP1 protein as coating antigen, Vserum：Vblocking solution = 1：50 dilution for serum and Vsecondary enzyme-linked antibedies：Vblocking solution ---1：2 000 for enzyme combined antibodies. The results showod that the sensitivity and specificity of this method were 94.32% and 99.09% respectively, the coefficients of variations in intra-assay and inter-assay reproducibility tests was lower than 8%. Compared with liquid phase blocking ELISA kits, the agreement of 201 serum samples reached 92.54%. The VP1-ELISA method established here is specific, sensitive, stable and simple, which can be used to monitor the antibody level of FMD serotype A.

关键词： Foot and mouth disease virus serotype A VP1 protein Indirect ELISA

Fetal and neonatal programming of postnatal growth and feed efficiency in swine

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Journal of animal Science and Biotechnology 2017年第4期8卷 764-778页

作者： Yun Ji Zhenlong Wu Zhaolai Dai Xiaolong Wang Ju Li Binggen Wang Guoyao Wu State key laboratory of animal nutrition China Agricultural UniversityBeijing 100193 China Henan Yinfa animal Husbandry Co. Ltd. XinzhengHenan 451100 China Department of animal Science and Center for animal Genomics Texas A&M University Room 212 College Station TX 77843 USA.

Maternal undernutrition or overnutrition during pregnancy alters organ structure, impairs prenatal and neonatal growth and development, and reduces feed efficiency for lean tissue gains in pigs. These adverse effects may be carried over to the next generation or beyond. This phenomenon of the transgenerational impacts is known as fetal programming, which is mediated by stable and heritable alterations of gene expression through covalent modifications of DNA and histones without changes in DNA sequences(namely, epigenetics). The mechanisms responsible for the epigenetic regulation of protein expression and functions include chromatin remodeling; DNA methylation(occurring at the 5′-position of cytosine residues within CpG dinucleotides); and histone modifications(acetylation, methylation, phosphorylation, and ubiquitination). Like maternal malnutrition, undernutrition during the neonatal period also reduces growth performance and feed efficiency(weight gain:feed intake; also known as weightgain efficiency) in postweaning pigs by 5–10%, thereby increasing the days necessary to reach the market bodyweight. Supplementing functional amino acids(e.g., arginine and glutamine) and vitamins(e.g., folate) play a key role in activating the mammalian target of rapamycin signaling and regulating the provision of methyl donors for DNA and protein methylation. Therefore, these nutrients are beneficial for the dietary treatment of metabolic disorders in offspring with intrauterine growth restriction or neonatal malnutrition. The mechanism-based strategies hold great promise for the improvement of the efficiency of pork production and the sustainability of the global swine industry.

关键词： Epigenetics Fetal programming Gene expression Neonatal programming nutrition

Coordinated transcriptional and post-transcriptional epigenetic regulation during skeletal muscle development and growth in pigs

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Journal of animal Science and Biotechnology 2023年第2期14卷 564-577页

作者： Du Zhang Shumei Wu Xinxin Zhang Shuqiang Ren Zhonglin Tang Fei Gao Genome Analysis laboratory of the Ministry of Agriculture Agricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518000China College of animal Sciences South China Agricultural UniversityGuangdong Provincial Key Lab of Agro-animal Genomics and Molecular BreedingNational Engineering Research Center for Breeding Swine IndustryGuangzhou 510642China Kunpeng Institute of Modern Agriculture at Foshan Chinese Academy of Agricultural SciencesFoshan 528226China GuangXi Engineering Centre for Resource Development of Bama Xiang Pig Bama 547500China Comparative Pediatrics and nutrition Department of Veterinary and Animal SciencesFaculty of Health and Medical SciencesUniversity of CopenhagenCopenhagenDenmark.

Background:N6-methyladenosine(m^(6)A)and DNA 5-methylcytosine(5mC)methylation plays crucial roles in diverse biological processes,including skeletal muscle development and *** studies unveiled a potential link between these two systems,implicating the potential mechanism of coordinated transcriptional and post-transcrip-tional regulation in porcine prenatal myogenesis and postnatal skeletal muscle ***:Immunofluorescence and co-IP assays were carried out between the 5mC writers and m^(6)A writers to investigate the molecular basis ***-scale in-house transcriptomic data were compiled for applying weighted correlation network analysis(WGCNA)to identify the co-expression patterns of m^(6)A and 5mC regulators and their potential role in pig ***-genome bisulfite sequencing(WGBS)and methylated RNA immu-noprecipitation sequencing(MeRIP-seq)were performed on the skeletal muscle samples from Landrace pigs at four postnatal growth stages(days 30,60,120 and 180).Results:Significantly correlated expression between 5mC writers and m^(6)A writers and co-occurrence of 5mC and m^(6)A modification were revealed from public datasets of C2C12 *** protein-protein interactions between the DNA methylase and the m^(6)A methylase were observed in mouse myoblast ***,by analyzing tran-scriptome data comprising 81 pig skeletal muscle samples across 27 developmental stages,we identified a 5mC/m^(6)A epigenetic module eigengene and decoded its potential functions in pre-or post-transcriptional regulation in postnatal skeletal muscle development and growth of *** integrative multi-omics analyses on the WGBS methylome data and MeRIP-seq data for both m^(6)A and gene expression profiles revealed a genome/transcriptome-wide correlated dynamics and co-occurrence of 5mC and m^(6)A modifications as a consequence of 5mC/m^(6)A crosstalk in the postnatal myogenesis progress of ***,we identified a group of myogenesis-relat

关键词： DNA methylation Epigenetic modification Epigenomic analysis 5mC regulators m^(6)A methylation m^(6)A regulators Myogenesis

LncRNAs:Current understanding,future directions,and challenges

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animal Models and Experimental Medicine 2023年第6期6卷 505-507页

作者： Xiaolin Liang Xiangting Wang Department of Geriatrics Gerontology Institute of Anhui ProvinceCentre for Leading Medicine and Advanced Technologies of IHMThe First Affiliated HospitalDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhuiChina Anhui province key laboratory of Geriatric Immunology and nutrition Therapy HefeiAnhuiChina MOE key laboratory for Membraneless Organelles and Cellular Dynamics Anhui Provincial Engineering Research Center for Elderly Care ProductsUniversity of Science and Technology of ChinaHefeiAnhuiChina

1|CURRENT UNDERSTANDING OF LNCRNAS Long non-coding RNAs(lncRNAs)are a class of regulatory RNA molecules that have captured the attention of researchers in recent years due to their diverse roles in development,physiol... 详细信息

关键词： processes directions diverse