检索结果-南通市图书馆

Palindrome-mediated DNA nanotubes with cell-specific aptamers to improve targeted antitumor effects and reduce toxicity on non-small cell lung cancer

引用

Science China(Life Sciences) 2025年第2期68卷 454-466页

作者： Cheng Zheng Lanlan Song Chang Yu Lingye Zhu Jing Zhang Ning Wang Mengchu Liu Shini Li Liangxing Wang Zhifa Shen Xiaoying Huang Division of Pulmonary medicine the First Affiliated Hospital of Wenzhou Medical UniversityKey Laboratory of Interdiscipline and Translational MedicineWenzhou 325000China Intervention Department the First Affiliated Hospital of Wenzhou Medical UniversityWenzhou 325000China key laboratory of laboratory medicine Ministry of Education of Chinaand Zhejiang Provincial Key Laboratory of Medical GeneticsSchool of Laboratory Medicine and Life SciencesWenzhou Medical UniversityWenzhou 325035China

Chemotherapy is regarded as a widely used and effective treatment strategy for lung cancer,although most conventional chemotherapeutics cause severe toxic side-effects due to their indiscriminate attacks on both cancerous and normal *** nucleic acid nanomaterials are emerging as a promising drug delivery strategy,their clinical applications are limited by rapid degradation by nucleases and difficulties in targeting cancer *** this study,we have developed a Rhein-loaded aptamer-based DNA nanotube(DNT-S6@Rhein)for the targeted and efficient therapy of non-small cell lung *** the palindrome segments,two specified oligonucleotides were hybridized and folded into the well-defined nanotubes(DNT-S6),with the S6 aptamer distributed *** obtained nanotubes exhibited excellent serum stability and targeting ability towards A549 cells due to the firm structure and decoration of the S6 ***,as an antitumor drug and DNA intercalator,can be effectively inserted into the *** drug-loaded nanotubes rapidly disassembled in intracellular environment and then the released Rhein was found to activate cellular apoptotic process and significantly suppress proliferation,migration and invasion of A549 ***,DNT-S6@Rhein could efficiently accumulate in tumor regions,offering compelling therapeutic efficacy and biocompatibility under both in vitro and in vivo *** findings of this study provide a promising strategy for mitigating the inevitable systemic side-effects of chemotherapy and expand the potential application of DNA nanostructure on targeted drug delivery.

关键词： DNA nanotube self-assembly Rhein targeted drug delivery cancer therapy

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

AI empowers exploration of protein universe

引用

Science China(Life Sciences) 2025年第2期68卷 588-589页

作者： Wuke Wang Xingxu Huang Zhejiang Provincial key laboratory of Pancreatic Disease The First Affiliated Hospitaland Institute of Translational MedicineZhejiang University School of MedicineHangzhou 310029China

Deaminases which perform the function of converting cytosine to thymine are ubiquitous in nature with over 120,000 known variants,each exhibiting significant ***,gene editing technologies have remarkably revealed that these deaminases share similar ***,the mining of novel proteins has been conducted through sequence alignment and the categorization of conserved regions to identify functional modules within protein sequences.

关键词： functions powers alignment

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Exploring the potential of extrachromosomal DNA as a novel oncogenic driver

引用

Science China(Life Sciences) 2025年第1期68卷 144-157页

作者： Huanbo Zhu Longtao Huangfu Junbing Chen Jiafu Ji Xiaofang Xing key laboratory of Carcinogenesis and translational Research(Ministry of Education/Beijing) Division of Gastrointestinal Cancer Translational Research LaboratoryPeking University Cancer Hospital&InstituteBeijing 100142China Department of Gastrointestinal Cancer Center Peking University Cancer Hospital&InstituteBeijing 100142China Department of Liver Surgery&Transplantation Liver Cancer InstituteZhongshan HospitalFudan UniversityKey Laboratory of Carcinogenesis and Cancer InvasionMinistry of EducationShanghai200032China State key laboratory of Holistic Integrative Management of Gastrointestinal Cancers Beijing Key Laboratory of Carcinogenesis and Translational ResearchDivision of Gastrointestinal Cancer Translational Research LaboratoryPeking University Cancer Hospital&InstituteBeijing 100142China

Extrachromosomal DNA(ecDNA)is a form of circular DNA mostly found in tumor *** the typical chromosomal DNA,ecDNA is circular,self-replicating,and carries complete or partial gene *** ecDNA occurrence remains a rare event in cancer,recent studies have shown that oncogene amplification on ecDNA is widespread throughout many types of cancer,implying that ecDNA plays a central role in accelerating tumor *** has also been associated with increased tumor mutation burden,chromosomal instability,and even tumor microenvironment *** may be crucial in influencing tumor heterogeneity,drug sensitivity,oncogenic senescence,and tumor immunogenicity,leading to a worsening prognosis for tumor *** this way,several clinical trials have been conducted to investigate the importance of ecDNA in clinical *** this review,we summarize the biogenesis,characteristics,and current research methods of ecDNA,discuss the vital role of ecDNA-caused tumor heterogeneity in cancers,and highlight the potential role of ecDNA in cancer therapy.

关键词： extrachromosomal DNA cancer therapy tumor heterogeneity drug resistance tumor evolution

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Exploring the mechanism of Xiaoaiping Injection inhibiting autophagy in prostate cancer based on proteomics

引用

Chinese Journal of Natural medicines 2025年第1期23卷 64-76页

作者： Qiuping Zhang Qiuju Huang Zhiping Cheng Wei Xue Shoushi Liu Yunnuo Liao Xiaolan Li Xin Chen Yaoyao Han Dan Zhu Zhiheng Su Xin Yang Zhuo Luo Hongwei Guo Department of Traditional Chinese medicine the First Affiliated Hospital of Guangxi Medical University Guangxi key laboratory of Bioactive Molecules Research and Evaluation & College of Pharmacy Guangxi Medical University key laboratory of Longevity and Aging-related Diseases of Chinese Ministry of Education & Center for translational medicine Guangxi Medical University

Xiaoaiping(XAP) Injection demonstrates the anti-prostate cancer(PCa) effects, yet the underlying mechanism remains unclear. This study aims to investigate the impact of XAP on PCa and elucidate its mechanism of action. PCa cell proliferation was evaluated using a cell counting kit-8(CCK-8) assay. Cell apoptosis was assessed through Hoechst staining and Western blotting assays. Proteomics technology was employed to identify key molecules and significant signaling pathways modulated by XAP in PCa cells. To further validate potential key genes and important pathways, a series of assays were conducted, including acridine orange(AO)staining, transmission electron microscopy, and immunofluorescence assays. The molecular mechanism of XAP against PCa in vivo was examined using a PC3 xenograft mouse model. Results demonstrated that XAP significantly inhibited cell proliferation in multiple PCa cell *** C4-2 and prostate cancer cell line-3(PC3) cells, XAP induced cellular apoptosis, evidenced by reduced B-cell lymphoma 2(Bcl-2) levels and elevated Bcl-2-associated X(Bax) levels. Proteomic, immunofluorescence, and quantitative reverse transcription-polymerase chain reaction(q RT-PCR) investigations revealed a strong correlation between forkhead box O3a(Fox O3a) autophagic degradation and the anti-PCa action of XAP. XAP hindered autophagy by reducing the expression levels of autophagy-related protein 5(Atg5)/autophagy-related protein 12(Atg12) and enhancing Fox O3a expression and nuclear translocation. Furthermore,XAP exhibited potent anti-PCa action in PC3 xenograft mice and triggered Fox O3a nuclear translocation in tumor tissue. These findings suggest that XAP induces PCa apoptosis via inhibition of Fox O3a autophagic degradation, potentially offering a novel perspective on XAP injection as an effective anticancer therapy for PCa.

关键词： Apoptosis Autophagy Forkhead box O3a Prostate cancer Xiaoaiping Injection

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Xiangshao Granules Ameliorate Post-stroke Depression by Inhibiting Activation of Microglia and IDO1 Expression in Hippocampus and Prefrontal Cortex

引用

Chinese Journal of Integrative medicine 2025年第1期31卷 28-38页

作者： LI Cheng-gang XU Lu-shan SUN Liang XU Yu-hao CAO Xiang ZHAO Chen-chen XIA Sheng-nan ZHANG Qing-xiu XU Yun Department of Neurology Nanjing Drum Tower HospitalAffiliated Hospital of Medical SchoolNanjing UniversityNanjing210008China State key laboratory of Pharmaceutical Biotechnology and Institute of translational medicine for Brain Critical Diseases Nanjing UniversityNanjing210008China Jiangsu key laboratory for Molecular medicine Medical School of Nanjing UniversityNanjing210008China Nanjing Neurology Medical Center Nanjing210008China

Objective To investigate the therapeutic effect of Xiangshao Granules(XSG)on post-stroke depression(PSD)and explore the underlying *** Forty-three C57BL/6J mice were divided into 3 groups:sham(n=15),PSD+vehicle(n=14),and PSD+XSG(n=14)groups according to a random number *** PSD models were constructed using chronic unpredictable mild stress(CUMS)after middle cerebral artery occlusion(MCAO).The sham group only experienced the same surgical operation,but without MACO and CUMS *** XSG group received XSG(60 mg/kg per day)by gavage for 4 *** mice in the sham and vehicle groups were given the same volume of 0.9%saline at the same *** body weight and behavior tests including open field test,sucrose preference test,tail suspension test,and elevated plus-maze test,were used to validate the PSD mouse ***-time fluorescence quantitative polymerase chain reaction(RT-qPCR),enzyme-linked immunosorbent assay(ELISA),and immunofluorescence staining were used to evaluate the anti-inflammatory effects of *** potential molecular mechanisms were explored and verified through network pharmacology analysis,Nissl staining,Western blot,ELISA,and RT-qPCR,*** The body weight and behavior tests showed that MCAO combined with CUMS successfully established the PSD *** alleviated neuronal damage,reduced the expressions of pro-apoptotic proteins Caspase-3 and B-cell lymphoma-2(BCL-2)-associated X(BAX),and increased the expression of anti-apoptotic protein BCL-2 in PSD mice(P<0.05 or P<0.01).XSG inhibited microglial activation and the expressions of pro-inflammatory cytokines including tumor necrosis factor-α,interleukin(IL)-1β,and IL-6 via the toll-like receptor 4/nuclear factor kappa-B signaling pathway in PSD mice(P<0.05 or P<0.01).Furthermore,XSG decreased the expression of indoleamine 2,3-dioxygenase1(IDO1)and increased the concentration of 5-hydroxytryptamine in PSD mice(P<0.05 or P<0.01).Conclusion XSG could reverse

关键词： post-stroke depression Xiangshao Granules indoleamine 2,3-dioxygenase 1 Chinese medicine

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Phosphodiesterase 9 localization in cytoplasm and nucleus:the gateway to selective targeting in neuroprotection?

引用

Neural Regeneration Research 2025年第6期20卷 1701-1702页

作者： Giovanni Ribaudo Matteo Giannangeli Margrate Anyanwu Alessandra Gianoncelli Department of Molecular and translational medicine University of BresciaBresciaItaly

The umbrella term"neurodege ne rative disorders"(NDDs) refers to several conditions characterized by a progressive loss of structure and function of cells belonging to the nervous *** diseases affect more than 50million people *** disorders are characterized by sundry factors and pathophysiological mechanisms that a re challenging to be fully *** of these rely on cell signaling pathways to preserve homeostasis,involving second messengers such as cyclic adenosine monophosphate (cAMP)and cyclic guanosine 3',5'-monophosphate(cGMP).Their ability to control the duration and amplitude of the signaling cascade is given by the presence of several common and uncommon *** kinases A and G (PKA and PKG),phosphodiesterases (PDEs),and scaffold proteins are among them.

关键词： gateway homeostasis preserve

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Continuum estimation in low-resolution gamma-ray spectra based on deep learning

引用

Nuclear Science and Techniques 2025年第2期36卷 5-17页

作者： Ri Zhao Li-Ye Liu Xin Liu Zhao-Xing Liu Run-Cheng Liang Ren-Jing Ling-Hu Jing Zhang Fa-Guo Chen China Institute for Radiation Protection Taiyuan 030006China Shanxi Provincial key laboratory for translational Nuclear medicine and Precision Protection Taiyuan 030006China

In this study,an end-to-end deep learning method is proposed to improve the accuracy of continuum estimation in low-resolution gamma-ray spectra.A novel process for generating the theoretical continuum of a simulated spectrum is established,and a convolutional neural network consisting of 51 layers and more than 105 parameters is constructed to directly predict the entire continuum from the extracted global spectrum *** testing,an in-house NaI-type whole-body counter is used,and 106 training spectrum samples(20%of which are reserved for testing)are generated using Monte Carlo *** addition,the existing fitting,step-type,and peak erosion methods are selected for *** proposed method exhibits excellent performance,as evidenced by its activity error distribution and the smallest mean activity error of 1.5%among the evaluated ***,a validation experiment is performed using a whole-body counter to analyze a human physical phantom containing four *** largest activity error of the proposed method is−5.1%,which is considerably smaller than those of the comparative methods,confirming the test *** multiscale feature extraction and nonlinear relation modeling in the proposed method establish a novel approach for accurate and convenient continuum estimation in a low-resolution gamma-ray ***,the proposed method is promising for accurate quantitative radioactivity analysis in practical applications.

关键词： Gamma-ray spectrum Continuum estimation Deep learning Convolutional neural network End-to-end prediction

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Treating incurable non-communicable diseases by targeting iron metabolism and ferroptosis

引用

Science China Life Sciences 2025年

作者： Ruoxuan Ni Jianwei Jiang Fudi Wang Junxia Min The First Affiliated Hospital Institute of Translational Medicine Zhejiang University School of Medicine The Second Affiliated Hospital School of Public Health State Key Laboratory of Experimental Hematology Zhejiang University School of Medicine

Both iron metabolism and ferroptosis (an iron-dependent form of programmed cell death) have been connected to the development and progression of many currently incurable non-communicable diseases, including Alzheimer’s disease, Parkinson’s disease, multiple sclerosis,Huntington’s disease, metabolic dysfunction-associated steatohepatitis, heart failure, and both treatment-relapsed and refractory cancers,such as pancreatic ductal adenocarcinoma and triple-negative breast cancer. Thus, understanding the relationship between iron and these diseases can pave the way for the development of novel therapeutic strategies. Here, we summarize the latest evidence supporting the pathological roles of dysregulated iron metabolism and ferroptosis in a wide range of preclinical animal models of these currently incurable non-communicable diseases. We also summarize the feasibility of targeting iron metabolism and ferroptosis for the prevention and treatment of iron-and ferroptosis-related diseases that currently have limited treatment options. In addition, we provide our perspectives on the challenges and promises regarding the translational potential of targeting dysregulated iron metabolism and ferroptosis to treat diseases,highlighting the future roadmap for developing iron-and ferroptosis-targeted therapeutics.

关键词：

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

The burden of upper motor neuron involvement is correlated with the bilateral limb involvement interval in patients with amyotrophic lateral sclerosis:a retrospective observational study

引用

Neural Regeneration Research 2025年第5期20卷 1505-1512页

作者： Jieying Wu Shan Ye Xiangyi Liu Yingsheng Xu Dongsheng Fan Department of Neurology Peking University Third HospitalBeijingChina Beijing Municipal key laboratory of Biomarker and translational Research in Neurodegenerative Diseases BeijingChina key laboratory for Neuroscience National Health Commission/Ministry of EducationPeking UniversityBeijingChina

Amyotrophic lateral sclerosis is a rare neurodegenerative disease characterized by the involvement of both upper and lower motor *** bilateral limb involvement significantly affects patients'daily lives and may lead them to be confined to ***,the effect of upper and lower motor neuron impairment and other risk factors on bilateral limb involvement is *** address this issue,we retrospectively collected data from 586 amyotrophic lateral sclerosis patients with limb onset diagnosed at Peking University Third Hospital between January 2020 and May 2022.A univariate analysis revealed no significant differences in the time intervals of spread in different directions between individuals with upper motor neuron-dominant amyotrophic lateral sclerosis and those with classic amyotrophic lateral *** used causal directed acyclic graphs for risk factor determination and Cox proportional hazards models to investigate the association between the duration of bilateral limb involvement and clinical baseline characteristics in amyotrophic lateral sclerosis *** factor analyses revealed that higher upper motor neuron scores(hazard ratio[HR]=1.05,95%confidence interval[CI]=1.01–1.09,P=0.018),onset in the left limb(HR=0.72,95%CI=0.58–0.89,P=0.002),and a horizontal pattern of progression(HR=0.46,95%CI=0.37–0.58,P<0.001)were risk factors for a shorter interval until bilateral limb *** results demonstrated that a greater degree of upper motor neuron involvement might cause contralateral limb involvement to progress more quickly in limb-onset amyotrophic lateral sclerosis *** findings may improve the management of amyotrophic lateral sclerosis patients with limb onset and the prediction of patient prognosis.

关键词： amyotrophic lateral sclerosis bilateral limb involvement Cox proportional hazards regression model horizontal spread restricted cubic spline analysis time interval upper motor neuron vertical spread

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Immune cells:potential carriers or agents for drug delivery to the central nervous system

引用

Military Medical Research 2025年第1期12卷 121-153页

作者： Shan-Shan Zhang Ruo-Qi Li Zhong Chen Xiao-Ying Wang Aaron S.Dumont Xiang Fan School of Basic Medical Sciences Zhejiang Chinese Medical UniversityHangzhou 310053China key laboratory of Neuropharmacology and translational medicine of Zhejiang Province Zhejiang Chinese Medical UniversityHangzhou 310053China Clinical Neuroscience Research Center Department of Neurosurgery and NeurologyTulane University School of MedicineNew OrleansLA 70122USA

Drug delivery systems(DDS)have recently emerged as a promising approach for the unique advantages of drug protection and targeted ***,the access of nanoparticles/drugs to the central nervous system(CNS)remains a challenge mainly due to the obstruction from brain *** cells infiltrating the CNS in the pathological state have inspired the development of strategies for CNS foundation drug ***,we outline the three major brain barriers in the CNS and the mechanisms by which immune cells migrate across the blood–brain *** subsequently review biomimetic strategies utilizing immune cell-based nanoparticles for the delivery of nanoparticles/drugs to the CNS,as well as recent progress in rationally engineering immune cell-based DDS for CNS ***,we discuss the challenges and opportunities of immune cell-based DDS in CNS diseases to promote their clinical development.

关键词： Drug delivery systems Immune cells Blood-brain barrier Central nervous system

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：