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Electroactive and antibacterial wound dressings based on Ti_(3)C_(2)T_(x)MXene/poly(ε-caprolactone)/gelatin coaxial electrospun nanofibrous membranes

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Nano Research 2023年第7期16卷 9672-9687页

作者： Shixin Xu Chen Du Miaomiao Zhang Ruoying Wang Wei Feng Chengwei Wang Qinsong Liu Wen Zhao key laboratory for space biosciences and biotechnology School of Life SciencesNorthwestern Polytechnical UniversityXi’an 710072China

Endogenous electric fields(EFs)are capable of regulating the behaviors of skin cells in wound healing.However,majority of current dressings are primarily engaged in the passive repair of defective tissue,as they lack the ability to actively respond to physiological electrical signals.In this work,a series of nanofibrous membranes(NFMs)were fabricated by coaxial electrospinning,combining the good mechanical properties of poly(ε-caprolactone)(PCL),the bioactivity of gelatin and the electroactivity of Ti_(3)C_(2)T_(x)MXene,as electroactive and antibacterial dressings for cutaneous wound healing.The obtained NFMs exhibited suitable mechanical properties and hydrophilicity,excellent electroactivity,antibacterial activity,and biocompatibility.Especially,Ti_(3)C_(2)T_(x)MXene/PCL/gelatin-6(MPG-6,6 wt.%of Ti_(3)C_(2)T_(x)MXene in sheath spinning liquids)showed the optimal conductivity and antibacterial activity.Excitingly,this scaffold significantly promoted the adhesion,proliferation,and migration of NIH 3T3 cells under the electrical stimulation(ES).The in vivo evaluation in a full-thickness wounds defect model demonstrated that the MPG-6 films significantly accelerated wound closure,increased granulation tissue formation,increased collagen deposition,and promoted wound vascularization.In summary,the versatile scaffold is expected to be an ideal candidate as wound dressings due to its ability to promote the transmission of physiological electrical signals and thus improved the therapeutic outcomes of wound regeneration.

关键词： electroactive nanofibers coaxial electrospinning nanofibrous membranes(NFMs) antibacterial activity wound dressings

The role of 5′-adenosine monophosphate-activated protein kinase(AMPK)in skeletal muscle atrophy

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BIOCELL 2023年第2期47卷 269-281页

作者： KAI DANG HAFIZ MUHAMMAD UMER FAROOQ YUAN GAO XIAONI DENG AIRONG QIAN laboratory for Bone Metabolism Xi’an Key Laboratory of Special Medicine and Health EngineeringKey Laboratory for Space Biosciences and BiotechnologyResearch Center for Special Medicine and Health Systems EngineeringNPU-UAB Joint Laboratory for Bone MetabolismSchool of Life SciencesNorthwestern Polytechnical UniversityXi’an710072China

As a key coordinator of metabolism,AMP-activated protein kinase(AMPK)is vitally involved in skeletal muscle maintenance.AMPK exerts its cellular effects through its function as a serine/threonine protein kinase by regulating many downstream targets and plays important roles in the development and growth of skeletal muscle.AMPK is activated by phosphorylation and exerts its function as a kinase in many processes,including synthesis and degradation of proteins,mitochondrial biogenesis,glucose uptake,and fatty acid and cholesterol metabolism.Skeletal muscle atrophy is a result of various diseases or disorders and is characterized by a decrease in muscle mass.The pathogenesis and therapeutic strategies of skeletal muscle atrophy are still under investigation.In this review,we discuss the role of AMPK in skeletal muscle metabolism and atrophy.We also discuss targeting AMPK for skeletal muscle treatment,including exercise,AMPK activators including 5-amino-4-imidazolecarboxamide ribonucleoside and metformin,and low-level lasers.These studies show the important roles of AMPK in regulating muscle metabolism and function;thus,the treatment of skeletal muscle atrophy needs to take into account the roles of AMPK.

关键词： AMPK,Autophagy Protein degradation,Protein synthesis,Skeletal muscle atrophy,Ubiquitin

Microtubule actin crosslinking factor 1 functions as a novel therapeutic target in lung metastasis of osteosarcoma

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Genes & Diseases 2023年第2期10卷 325-328页

作者： Chaofei Yang Ye Tian Fan Zhao Qian Huang Zhiyong Liu Chenyang He Hui Li Yu li Zhiping Miao Airong Qian lab for Bone Metabolism Xi'an Key Laboratory of Special Medicine and Health EngineeringKey Lab for Space Biosciences and BiotechnologyResearch Center for Special Medicine and Health Systems EngineeringNPU-UAB Joint Laboratory for Bone MetabolismSchool of Life SciencesNorthwestern Polytechnical UniversityXi'anShaanxi 710072China Department of Oncology The Second Affiliated Hospital of Xi'an Jiaotong UniversityXi'anShaanxi 710004China Honghui Hospital Xi'anShaanxi 710054China

Lung metastasis is the primary cause of death in osteosarcoma(OS)patients.1 A better understanding of the molecular mechanisms underlying OS tumorigenesis and metastasis is urgently needed to identify therapeutic targets.Microtubule actin crosslinking factor 1(MACF1),which belongs to the spectraplakin family of cytoskeletal crosslinking proteins,2 is critical for cell migration and polarization due to its regulation of the cytoskeleton.Recently,MACF1 was indicated to be involved in the metastatic invasion of some human cancers,3 but the function of MACF1 in OS is still unclear.

关键词： metastasis osteosarcoma therapeutic

An integrated genome-wide analysis identifies HUR/ELAVL1 as a positive regulator of osteogenesis through enhancing theβ-catenin signaling activity

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Genes & Diseases 2023年第2期10卷 377-380页

作者： Ying Huai Zhihao Chen Xiaoni Deng Xue Wang Wenjing Mao Zhiping Miao Yu Li Hui Li Xiao Lin Airong Qian lab for Bone Metabolism Xi'an Key Laboratory of Special Medicine and Health EngineeringSchool of Life SciencesNorthwestern Polytechnical UniversityXi'anShaanxi 710072China key lab for space biosciences and biotechnology School of Life SciencesNorthwestern Polytechnical UniversityXi'anShaanxi 710072China Research Center for Special Medicine and Health Systems Engineering School of Life SciencesNorthwestern Polytechnical UniversityXi'anShaanxi 710072China NPU-UAB Joint laboratory for Bone Metabolism School of Life SciencesNorthwestern Polytechnical UniversityXi'anShaanxi 710072China Honghui Hospital Xi'an Jiaotong University College of MedicineXi'anShaanxi 710054China

Osteoporosis is a prevalent multifactorial bone disease with a strong genetic contribution.The heritability of traits that contribute to osteoporosis(bone mass,bone mineral density(BMD),bone size,bone loss and fractures)ranges from 50 to 85%,suggesting that a comprehensive understanding of the genetic basis may help identify new therapeutic targets.1 However,the genetic characteristics remain obscure,and the existing drug targets are associated with various challenges.Numerous studies have demonstrated that high-throughput sequencing data analysis is fruitful for identifying novel targets of human diseases.2 We therefore integrated GWAS and transcriptome analyses through Multi-marker Analysis of GenoMic Annotation(MAGMA)and weighted gene co-expression network analysis3(WGCNA)to identify new network modules and potential therapeutic genes for osteoporosis.As an illustration,the flow chart presenting the process of the present study was shown in Figure S1.

关键词： osteoporosis analysis Figure

Bioengineered miR-27b-3p and miR-328-3p modulate drug metabolism and disposition via the regulation of target ADME gene expression

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Acta Pharmaceutica Sinica B 2019年第3期9卷 639-647页

作者： Xin Li Ye Tian Mei-Juan Tu Pui Yan Ho Neelu Batra Ai-Ming Yu key laboratory of Molecular Target&Clinical Pharmacology School of Pharmaceutical Sciences&the Fifth Affiliated HospitalGuangzhou Medical UniversityGuangzhou511436China lab for Bone Metabolism Key Lab for Space Biosciences and BiotechnologySchool of Life SciencesNorthwestern PolytechnicalUniversityXi'an710072China Department of Biochemistry&Molecular Medicine UC Davis School of MedicineSacramentoCA95817USA

Drug-metabolizing enzymes, transporters, and nuclear receptors are essential for the absorption, distribution, metabolism, and excretion(ADME) of drugs and xenobiotics. MicroRNAs participate in the regulation of ADME gene expression via imperfect complementary Watson–Crick base pairings with target transcripts. We have previously reported that Cytochrome P450 3A4(CYP3A4) and ATP-binding cassette sub-family G member 2(ABCG2) are regulated by miR-27b-3p and miR-328-3p,respectively. Here we employed our newly established RNA bioengineering technology to produce bioengineered RNA agents(BERA), namely BERA/miR-27b-3p and BERA/miR-328-3p, via fermentation. When introduced into human cells, BERA/miR-27b-3p and BERA/miR-328-3p were selectively processed to target miRNAs and thus knock down CYP3A4 and ABCG2 mRNA and their protein levels,respectively, as compared to cells treated with vehicle or control RNA. Consequently, BERA/miR-27b-3p led to a lower midazolam 10-hydroxylase activity, indicating the reduction of CYP3A4 activity. Likewise,BERA/miR-328-3p treatment elevated the intracellular accumulation of anticancer drug mitoxantrone, a classic substrate of ABCG2, hence sensitized the cells to chemotherapy. The results indicate that biologic miRNA agents made by RNA biotechnology may be applied to research on miRNA functions in the regulation of drug metabolism and disposition that could provide insights into the development of more effective therapies.

关键词： Bioengineered RNA miR-27b miR-328 CYP3A4 ABCG2 Drug disposition

Global gene expression profiling of blast lung injury of goats exposed to shock wave

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Chinese Journal of Traumatology 2020年第5期23卷 249-257页

作者： Hong Wang Wen-Juan Zhang Jun-Hong Gao Jin-Ren Liu Zhi-Yong Liu Bao-Qing Xia Xiao-Lin Fan Cun-Zhi Li Ai-Rong Qian lab for Bone Metabolism Xi'an Key Laboratory of Special Medicine and Health EngineeringKey Lab for Space Biosciences and BiotechnologyResearch Center for Special Medicine and Health Systems EngineeringNPU-UAB Joint Laboratory for Bone MetabolismSchool of Life SciencesNorthwestern Polytechnical UniversityXi'an 710072China Research Center for Toxicological and Biological Effects Institute for Hygiene of Ordnance IndustryXi'an 710065China

Purpose:Blast lung injury(BLI)is the most common damage resulted from explosion-derived shock wave in military,terrorism and industrial accidents.However,the molecular mechanisms underlying BLI induced by shock wave are still unclear.Methods:In this study,a goat BLI model was established by a fuel air explosive power.The key genes involved in were identified.The goats of the experimental group were fixed on the edge of the explosion cloud,while the goats of the control group were 3 km far away from the explosive environment.After successful modeling for 24 h,all the goats were sacrificed and the lung tissue was harvested for histopathological observation and RNA sequencing.Gene ontology(GO)and kyoto encyclopedia of genes and genomes(KEGG)analysis were performed to identify the main enriched biological functions of differentially expressed genes(DEGs).Quantitative real-time polymerase chain reaction(qRT-PCR)was used to verify the consistency of gene expression.Results:Of the sampled goat lungs,895 genes were identified to be significantly differentially expressed,and they were involved in 52 significantly enriched GO categories.KEGG analysis revealed that DEGs were highly enriched in 26 pathways,such as cytokine-cytokine receptor interaction,antifolate resistance,arachidonic acid metabolism,amoebiasis and bile secretion,JAK-STAT,and IL-17 signaling pathway.Furthermore,15 key DEGs involved in the biological processes of BLI were confirmed by qRTPCR,and the results were consistent with RNA sequencing.Conclusion:Gene expression profiling provide a better understanding of the molecular mechanisms of BLI,which will help to set strategy for treating lung injury and preventing secondary lung injury induced by shock wave.

关键词： Blast lung injury Shock wave Differentially expressed genes RNA sequencing Transcriptome

Mechanical Sensitive Molecule MACF1 Promotes Osteoblast Differentiation

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医用生物力学 2019年第A1期34卷 71-72页

作者： Lifang Hu Chong Yin Zixiang Wu Zizhang Huang Peihong Su Yan Zhang Zhihao Chen Airong Qian lab for Bone Metabolism Key Lab for Space Biosciences and BiotechnologyResearch Center for Special Medicine and Health Systems EngineeringNPU-UAB Joint Laboratory for Bone MetabolismSchool of Life SciencesNorthwestern Polytechnical University

The decreased osteoblast differentiation associated with reduced bone formation is one main cause of microgravityinduced bone loss.Our previous studies have demonstrated that microtubule actin crosslinking factor 1(MACF1)is downregulated in association with the decreased osteoblast differentiation and bone formation under simulated microgravity conditions.These findings suggest that MACF1 is sensitive to mechanical condition and may be critical for osteoblast differentiation and bone formation.To verify this hypothesis,current study investigates the role and mechanism of MACF1 in regulatingosteoblast differentiation by adopting MACF1 knockdown(MACF1-KD)osteoblasts.The results showed that MACF1 knockdown suppressed mineralized nodules formation,alkaline phosphatase(ALP)activity,osteogenic gene expression andβ-catenin signaling transduction.Moreover,we used RNA sequencing(RNA-seq)and chromatin immunoprecipitation sequencing(ChIP-seq)to investigate further mechanism.Interestingly,we found that MACF1 sequesterd repressors of osteoblast differentiation in cytoplasm.In conclusion,MACF1 is sensitive to mechanical condition and plays key role in activatingβ-catenin signaling transduction and sequestering repressors of osteoblast differentiation,which further promotes osteoblast differentiation.

关键词： MACF1 osteoblast cell differentiation β-catenin signaling

The Possibility of Changing the Wettability of Material Surface by Adjusting Gravity

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Research 2020年第1期2020卷 435-445页

作者： Yong-Ming Liu Zi-Qing Wu Sheng Bao Wei-Hong Guo Da-Wei Li Jin He Xiang-Bin Zeng Lin-Jun Huang Qin-Qin Lu Yun-Zhu Guo Rui-Qing Chen Ya-Jing Ye Chen-Yan Zhang Xu-Dong Deng Da-Chuan Yin key lab of space Bioscience&biotechnology School of Life SciencesNorthwestern Polytechnical UniversityXi’an710072 ShaanxiChina School of Bioengineering Sichuan University of Science and EngineeringZigong643000 SichuanChina Shenzhen Research Institute of Northwestern Polytechnical University Shenzhen518057 GuangdongChina

The contact angle,as a vital measured parameter of wettability of material surface,has long been in dispute whether it is affected by gravity.Herein,we measured the advancing and receding contact angles on extremely low contact angle hysteresis surfaces under different gravities(1-8G)and found that both of them decrease with the increase of the gravity.The underlying mechanism is revealed to be the contact angle hysteresis and the deformation of the liquid-vapor interface away from the solid surface caused by gradient distribution of the hydrostatic pressure.The real contact angle is not affected by gravity and cannot measured by an optical method.The measured apparent contact angles are angles of inclination of the liquid-vapor interface away from the solid surface.Furthermore,a new equation is proposed based on the balance of forces acting on the three-phase contact region,which quantitatively reveals the relation of the apparent contact angle with the interfacial tensions and gravity.This finding can provide new horizons for solving the debate on whether gravity affects the contact angle and may be useful for the accurate measurement of the contact angle and the development of a new contact angle measurement system.

关键词： apparent hysteresis hydrostatic

A dual plasmonic core-shell Pt/[TiN@TiO_(2)]catalyst for enhanced photothermal synergistic catalytic activity of VOCs abatement

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Nano Research 2022年第8期15卷 7071-7080页

作者： Anqi Li Qianpeng Zhang Shuaiqi Zhao Yanan Chong Peng Wu Yifei Li Xiaojing Jin Guangxu Chen Yongcai Qiu Shihe Yang Daiqi Ye Guangdong Provincial key laboratory of Atmospheric Environment and Pollution Control School of Environment and EnergySouth China University of TechnologyGuangzhou 510006China Department of Electronic and Computer Engineering The Hong Kong University of Science and TechnologyClear Water BayKowloonHong KongChina Guangzhou HKUST Fok Ying Tung Research Institute NanshaGuangzhou 511458China State key laboratory of Luminescent Materials and Devices South China University of TechnologyGuangzhou 510640China Guangdong key lab of Nano-Micro Material Research School of Chemical Biology and Biotechnology Shenzhen Graduate SchoolPeking UniversityShenzhen 518055China

Volatile organic compounds(VOCs)are ubiquitous organic pollutants affecting atmospheric environment and human health.The development of new efficient and environmentally friendly materials utilizing photothermal synergistic catalysis for purification of VOCs is still challenging.Herein,we design and prepare a core–shell TiN@TiO_(2)nanostructure integrating with nanoscaled Pt(Pt/[TiN@TiO_(2)])by an attractive quenching method.The strong light-harvesting capability of Pt and TiN components improve light-to-heat utilization efficiency by their intrinsic surface plasmon resonance effect.The TiO_(2)component upon the surface and the coexisting coupling effect of Pt0 and Pt2+enhance the photocatalytic effect of the system.As a result,the catalytic performance is significantly improved with toluene(120 ppm)conversion of 100%under the gas hourly space velocity of 72,000 mL·g^(−1)·h^(−1)and light illumination of 500 mW·cm^(−2).The desired catalyst thus achieves highly efficient coupling effect of photocatalysis and light-to-heat conversion for promoting VOCs abatement.

关键词： core-shell Pt/[TiN@TiO_(2)]catalyst volatile organic compounds(VOCs)abatement photothermal synergistic catalysis full spectrum sunlight utilization