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Chronic corticosterone disrupts the circadian rhythm of CRH expression and m^(6)A RNA methylation in the chicken hypothalamus

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Journal of animal Science and Biotechnology 2022年第4期13卷 1056-1068页

作者： Yang Yang Wanwan Han Aijia Zhang Mindie Zhao Wei Cong Yimin Jia Deyun Wang Ruqian Zhao MOE Joint International Research laboratory of animal Health&Food Safety Institute of ImmunologyNanjing Agricultural UniversityNanjing 210095People’s Republic of China key laboratory of animal physiology&biochemistry College of Veterinary MedicineNanjing Agricultural UniversityNanjing 210095People’s Republic of China Institute of Traditional Chinese Veterinary Medicine College of Veterinary MedicineNanjing Agricultural UniversityNanjing 210095People’s Republic of China.

Background:Corticotropin-releasing hormone(CRH),the major secretagogue of the hypothalamic-pituitary-adrenal(HPA)axis,is intricately intertwined with the clock genes to regulate the circadian rhythm of various body functions.N6-methyladenosine(m^(6)A)RNA methylation is involved in the regulation of circadian rhythm,yet it remains unknown whether CRH expression and m^(6)A modification oscillate with the clock genes in chicken hypothalamus and how the circadian rhythms change under chronic ***:Chronic exposure to corticosterone(CORT)eliminated the diurnal patterns of plasma CORT and melatonin levels in the *** circadian rhythms of clock genes in hippocampus,hypothalamus and pituitary are all disturbed to different extent in CORT-treated *** most striking changes occur in hypothalamus in which the diurnal fluctuation of CRH mRNA is flattened,together with mRNA of other feeding-related ***,hypothalamic m^(6)A level oscillates in an opposite pattern to CRH mRNA,with lowestm^(6)A level after midnight(ZT18)corresponding to the peak of CRH mRNA before dawn(ZT22).CORT diminished the circadian rhythm of m^(6)A methylation with significantly increased level at *** site-specific m^(6)A analysis on 3’UTR of CRH mRNA indicates that higher m^(6)A on 3’UTR of CRH mRNA coincides with lower CRH mRNA at night(ZT18 and ZT22).Conclusions:Our results indicate that chronic stress disrupts the circadian rhythms of CRH expression in hypothalamus,leading to dysfunction of HPA axis in the *** m^(6)A modification is involved in the regulation of circadian rhythms in chicken hypothalamus under both basal and chronic stress conditions.

关键词： Chronic corticosterone exposure Circadian rhythms CRH Hypothalamus m^(6)A

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Constant light in early life induces fear-related behavior in chickens with suppressed melatonin secretion and disrupted hippocampal expression of clock-and BDNF-associated genes

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Journal of animal Science and Biotechnology 2022年第6期13卷 1799-1809页

作者： Yang Yang Wei Cong Jie Liu Mindie Zhao Peirong Xu Wanwan Han Deyun Wang Ruqian Zhao MOE Joint International Research laboratory of animal Health&Food Safety Institute of ImmunologyNanjing Agricultural UniversityNanjing 210095People’s Republic of China key laboratory of animal physiology&biochemistry College of Veterinary MedicineNanjing Agricultural UniversityNanjing 210095People’s Republic of China Institute of Traditional Chinese Veterinary Medicine College of Veterinary MedicineNanjing Agricultural UniversityNanjing 210095People’s Republic of China

Background: Light management plays an important role in the growth and behavior of broiler chickens. Constant light in early post hatch stage has been a common practice in broiler industry for improving growth performance,while whether and how constant light in early life affects the behavior of broiler chickens is rarely ***: In this study, newly hatched chicks were kept in either constant(24 L:0 D, LL) or(12 L:12 D, LD) photoperiod for 7 d and then maintained in 12 L:12 D thereafter until 21 days of age. Constant light increased the average daily feed intake but not the body weight, which led to higher feed conversion ratio. Chickens in LL group exhibited fear-related behaviors, which was associated with higher corticosterone, lower melatonin and 5-HT levels. Concurrently, constant light exposure increased the mRNA expression of clock-related genes and suppressed the expression of antioxidative genes in the hippocampus. Moreover, brain derived neurotrophic factor/extracel ular signal-regulated kinase(BDNF/ERK) pathway was suppressed in the hippocampus of chickens exposed to constant light in the first week post ***: These findings indicate that constant light exposure in early life suppress melatonin secretion and disrupts hippocampal expression of genes involved in circadian clock and BDNF/ERK pathway, thereby contributing to fear-related behaviors in the chicken.

关键词： BDNF/ERK Constant light Fear-related behavior Hippocampus

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Dietary betaine activates hepatic VTGⅡexpression in laying hens associated with hypomethylation of GR gene promoter and enhanced GR expression

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Journal of animal Science and Biotechnology 2018年第2期9卷 381-390页

作者： Nagmeldin A.Omer Yun Hu Yan Hu Abdulrahman A.Idriss Halima Abobaker Zhen Hou Haibo Dong Ruqian Zhao MOE Joint International Research laboratory of animal Health&Food Safety Nanjing Agricultural UniversityNanjing 210095People’s Republic of China key laboratory of animal physiology&biochemistry Nanjing Agricultural UniversityNanjing 210095People’s Republic of China Jiangsu Collaborative Innovation Centre of Meat Production and Processing Quality and Safety ControlNanjing 210095People’s Republic of China Poultry Institute Chinese Academy of Agriculture SciencesYangzhouJiangsuChina

Background: Vitellogenin(VTG) is a precursor of egg yolk proteins synthesized within the liver of oviparous vertebrates. Betaine is an important methyl donor that is reported to improve egg production of laying hens with an unclear mechanism. In the present study, we fed betaine-supplemented diet(0.5%) to laying hens for 4 wk and investigated its effect on VTGII expression in the ***: Betaine did not affect chicken weight, but significantly(P < 0.05) increased egg laying rate accompanied with a significant(P < 0.05) increase in hepatic concentration and plasma level of VTGI. Plasma estrogen level did not change,but the hepatic expression of estrogen receptor α(ERα) mR NA was significantly(P < 0.05) up-regulated. Betaine did not affect the protein content of ERα, but significantly(P < 0.05) increased hepatic expression of glucocorticoid receptor(GR)at both mR NA and protein levels. Also, ERα/GR interaction tended to be enhanced in the liver nuclear lysates of betainesupplemented hens as determined by co-immunoprecipitation. Furthermore, dietary betaine supplementation significantly increased(P < 0.05) the hepatic expression of methyl-transfer enzymes, such as BHMT, GNMT, and DNMT1,which was associated with higher SAM/SAH ratio and hypomethylation of GR promoter ***: Betaine activates hepatic VTGII expression in association with modified DNA methylation of GR gene promoter, GR expression and ERα/GR interaction. Activation of hepatic VTGII expression may contribute, at least partly, to improved egg production in betaine-supplemented hens.

关键词： Betaine Glucocorticoid receptor Hypomethylation Laying hens Vitellogenin

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Protein Quality Control in Plant Organelles:Current Progress and Future Perspectives

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Molecular Plant 2021年第1期14卷 95-114页

作者： Jing-Liang Sun Jin-Yu Li Mei-Jing Wang Ze-Ting Song Jian-Xiang Liu State key laboratory of Plant physiology and biochemistry College of Life SciencesZhejiang UniversityHangzhou 310027China

The endoplasmic reticulum,chloroplasts,and mitochondria are major plant organelles for protein synthesis,photosynthesis,metabolism,and energy *** homeostasis in these organelles,maintained by a balance between protein synthesis and degradation,is essential for cell functions during plant growth,development,and stress ***-encoded chloroplast-and mitochondrion-targeted proteins and ER-resident proteins are imported from the cytosol and undergo modification and maturation within their respective *** folding is an error-prone process that is influenced by both developmental signals and environmental cues;a number of mechanisms have evolved to ensure efficient import and proper folding and maturation of proteins in plant *** or damaged proteins with nonnative conformations are subject to degradation via complementary or competing pathways:intraorganelle proteases,the organelle-associated ubiquitin-proteasome system,and the selective autophagy of partial or entire *** proteins in nonnative conformations accumulate,the organellespecific unfolded protein response operates to restore protein homeostasis by reducing protein folding demand,increasing protein folding capacity,and enhancing components involved in proteasome-associated protein degradation and *** review summarizes recent progress on the understanding of protein quality control in the ER,chloroplasts,and mitochondria in plants,with a focus on common mechanisms shared by these organelles during protein homeostasis.

关键词： autophagy chloroplast-associated protein degradation ER-associated protein degradation mitochondria-associated protein degradation protein quality control unfolded protein response

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A new genetic factor for root gravitropism in rice (Oryza sativa L.)

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2009年第10期10卷 777-783页

作者： Jiang-hua SHI Xi HAO Zhong-chang WU Ping WU State key laboratory of Plant physiology and biochemistry College of Life Sciences Zhefiang University Hangzhou 310058 China

Root gravitropism is one of the important factors to determine root architecture. To understand the mechanism underlying root gravitropism, we isolated a rice （Xiushui63） mutant defective in root gravitropism, designated as glsl. Vertical sections of root caps revealed that glsl mutant displayed normal distribution of amyloplast in the columella cells compared with the wild type. The glsl mutant was less sensitive to 2,4-dichlorophenoxyacetic acid （2,4-D） and ct-naphthaleneacetic acid （NAA） than the wild type. Genetic analysis indicated that the phenotype ofglsl mutant was caused by a single recessive mutation, which is mapped in a 255-kb region between RM16253 and CAPS1 on the short arm of chromosome 4.

关键词： Oryza sativa L. Root gravitropism Genetic factor

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Regulation of OsSPX1 and OsSPX3 on Expression of OsSPX Domain Genes and Pi-starvation Signaling in Rice

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Journal of Integrative Plant Biology 2009年第7期51卷 663-674页

作者： Zhiye Wang Han Hu Hongjie Huang Ke Duan Zhong changWu Ping Wu State key laboratory of Plant physiology and biochemistry College of Life Sciences Zhejiang University Hangzhou 310058 China

The rice （Oryza sativa L.） genome contains at least six genes exclusively with an SPX （SYG1/PHO81/XPR1） domain at the N-terminal, designated as OsSPX1-6. Here we report the diverse expression patterns of the OsSPX genes in different tissues and their responses to Pi-starvation. Among them, five genes, OsSPX1, 2, 3, 5 and 6 are responsive to Pi-starvation in shoots and/or in roots. The subcellular localization analysis indicates that OsSPX1 and OsSPX2 is exclusively located in nucleus, OsSPX3 in the cytoplasm, and OsSPX4 is a membrane localization protein. OsSPXI regulates OsSPX2, 3 and 5 at the transcription level and is positively involved in the responses of the genes to Pi-starvation. Overexpression of OsSPX3 downregulates OsSPX5 in shoots under Pi-sufficiency. OsSPX3 negatively regulates the PSI （Pi-starvation induced） gene, OslPS1 and is involved in the responses of miR399 and OsPH02 to Pi-starvation. Our results suggest that OsSPX1 may be a regulator involved in the transcriptions of OsSPX2, 3 and 5. OsSPX3 plays a role in OslPSllmiR399 mediated long distance regulation on OsPH02. Our results also indicate that OsSPX3 is involved in plant tolerance to Pi-starvation stress.

关键词： Oryza sativa SPX domain gene expression regulation Pi-signaling

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Identification and expression analysis of OsHsfs in rice

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2009年第4期10卷 291-300页

作者： Chuang WANG Qian ZHANG Hui-xia SHOU State key laboratory of Plant physiology and biochemistry College of Life Science Zhejiang University Hangzhou 310058 China

Heat stress transcription factors (Hsfs) are the central regulators of defense response to heat stress. We identified a total of 25 rice Hsf genes by genome-wide analysis of rice (Oryza sativa L.) genome, including the subspecies of O. japonica and O. indica. Proteins encoded by OsHsfs were divided into three classes according to their structures. Digital Northern analysis showed that OsHsfs were expressed constitutively. The expressions of these OsHsfs in response to heat stress and oxidative stress differed among the members of the gene family. Promoter analysis identified a number of stress-related cis-elements in the promoter regions of these OsHsfs. No significant correlation, however, was found between the heat-shock responses of genes and their cis-elements. Overall, our results provide a foundation for future research of OsHsfs function.

关键词： Heat shock Transcription factors Rice Protein structure Expression analysis

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bZIP17 regulates heat stress tolerance at reproductive stage in Arabidopsis

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aBIOTECH 2022年第1期3卷 1-11页

作者： Juan Gao Mei-ing Wang Jing-Jing Wang Hai-Ping Lu Jian-Xiang Liu State key laboratory of Plant physiology and biochemistry College of Life SciencesZhejiang UniversityHangzhou 310027China

High temperature elicits a well-conserved response called the unfol ded protein response(UPR)to bring protein homeostasis in the endoplasmic reticulum(ER).Two key UPR regulators bZIP28 and bZIP60 have been shown to be essential for maintaining fertility under heat stress conditions in Arabidopsis,however,the function of transcriptional activator bZIP17,a paralog of bZIP28,in heat stress response at reproductive stage is not *** we found that bzip17 mutant plants were sensitive to heat stress in terms of silique length and fertility comparing to that of wildtype(WT)Arabidopsis plants,and transcri ptomic analysis showed that 1380 genes were specifically up-regulated and 493 genes were specifically down-regulated by heat stress in the flowers of WT plants comparing to that in bzip17 mutant *** bZIP17-dependent up-regulated genes were enriched in responses to a biotic stresses such as water deprivation and salt *** chromatin immuno-preci pitation coupled with high-throughput sequencing(ChIP-Seq)uncovered 1645 genes that were direct targets of bZIP17 in MYC-bZIP17 expressing seedlings subjected to heat *** these 1645 genes,ERSE-II cis-element was enriched in the binding peaks of their promoters,and the up-regulation of 113 genes by heat stress in flowers was dependent on *** results revealed direct targets of bZIP17 in flowers during heat stress responses and demonstrated the important role of bZIP17 in maintai ning fertility upon heat stress in plants.

关键词： Arabidopsis bZIP17 Flower Fertility Heat Stress UPR

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DGP1,a drought-induced guard cell-specific promoter and its function analysis in tobacco plants

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Science China(Life Sciences) 2005年第2期48卷 181-186页

作者： LI Jun GONG Ximing LIN Huiqiong SONG Quanbo CHEN Jia WANG Xuechen State key laboratory of Plant physiology and biochemistry China Agricultural UniversityBeijing 100094China

The genetic regulation of stomatal movement mainly depends on an efficient control system of gene expression,and guard cell-specific promoter is becoming the best *** we combined the dehydration responsive element(DRE)with guard cell specific element(GCSE)to construct a novel promoter,*** assays in transgenic tobacco carryingβ-glucuronidase(gus)gene fused to DGP1 demonstrated that GUS activity was found to be highly inducible by drought treatment and specifically restricted to guard *** GUS activity was detected in roots,stems or flowers after *** quantitative analysis showed that GUS activity in the epidermal strips was apparently induced by dehydration and dramatically increased with the elongation of *** GUS activity after 8 h treatment was 179 times that of those without *** GUS activity in roots,stems or mesophyll increased after treatment,no great changes were *** results suggested that DGP1 could drive target gene expressed in guard cells when plant is subjected to drought *** this gets us prepared to control opening and closing of stomata through plant gene engineering.

关键词： tobacco promoter guard cell drought.

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Effects of the disaggregation of high-polymerized particles in guard cell vacuoles on osmoregulation of stomatal aperture (stomata opening)

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Chinese Science Bulletin 2003年第5期48卷 453-456页

作者： YE Qing, ZHU Guoli & LOU Chenghou Biology College, China Agricultural University, State key laboratory of Plant physiology and biochemistry, Beijing 100094, China Correspondence should be addressed to Zhu Guoli (e-mail: glzhu@mail. ***) Biology College China Agricultural University State Key Laboratory of Plant Physiology and Biochemistry

Observation under an electron microscope reveals that in closed and open stomata of V. faba, the aver-age volume of particles in guard cell vacuoles (GCV) reduces about 3 orders in magnitude, while the distribution density of the particles increases about 2 orders of magnitude. By using the method of the ratio of fluorescent emissions with laser scanning confocal microscopy, the monitoring to sto-mata opening shows that during 10 to 30 s before the first distinguishable aperture of stomata, there is a change of pH in GCV about -0.5 units. A quick stomatal opening immedi-ately follows the changes of pH in GCV to reach a steady aperture about 12 mm in 100200 s. This work proposes a model for the osmoregulation in GCV for stomatal opening. The proposed osmoregulation is related to the disaggregation of some polymerized particles inside GCV, which is probably induced by a -DpH in the vacuole. This model describes a process of osmoregulation that avoids the massive energy consuming transportation across cell membranes, which is a foundation of the current chemiosmotic hypothesis. This model is a supplement to the multiple controlling hypothesis for the stomatal movement, which widens research principle ideas for other quick movements in plants.

关键词：气孔运动气孔开放保卫细胞液泡渗透调节高聚合微粒解体

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