Effects of the disaggregation of high-polymerized particles in guard cell vacuoles on osmoregulation of stomatal aperture (stomata opening)

作     者：YE Qing, ZHU Guoli & LOU Chenghou Biology College, China Agricultural University, State Key Laboratory of Plant Physiology and Biochemistry, Beijing 100094, China Correspondence should be addressed to Zhu Guoli (e-mail: glzhu@mail. ***) 

作者机构：Biology College China Agricultural University State Key Laboratory of Plant Physiology and Biochemistry 

出 版 物：《Chinese Science Bulletin》 (中国科学通报)

年 卷 期：2003年第48卷第5期

页      面：453-456页

核心收录：

学科分类：09[农学] 0903[农学-农业资源与环境] 

基　　金：the National Natural Science Foundation of China (Grant Nos. 39870075 and 30170451) and The Major State Basic Research Development Program of China (Grant No. G19990117) 

主　　题：气孔运动 气孔开放 保卫细胞 液泡 渗透调节 高聚合微粒 解体 

摘      要：Observation under an electron microscope reveals that in closed and open stomata of V. faba, the aver-age volume of particles in guard cell vacuoles (GCV) reduces about 3 orders in magnitude, while the distribution density of the particles increases about 2 orders of magnitude. By using the method of the ratio of fluorescent emissions with laser scanning confocal microscopy, the monitoring to sto-mata opening shows that during 10 to 30 s before the first distinguishable aperture of stomata, there is a change of pH in GCV about -0.5 units. A quick stomatal opening immedi-ately follows the changes of pH in GCV to reach a steady aperture about 12 mm in 100200 s. This work proposes a model for the osmoregulation in GCV for stomatal opening. The proposed osmoregulation is related to the disaggregation of some polymerized particles inside GCV, which is probably induced by a -DpH in the vacuole. This model describes a process of osmoregulation that avoids the massive energy consuming transportation across cell membranes, which is a foundation of the current chemiosmotic hypothesis. This model is a supplement to the multiple controlling hypothesis for the stomatal movement, which widens research principle ideas for other quick movements in plants.