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Diagnostic performance and problem analysis of commercial tuberculosis antibody detection kits in China

Military Medical Research 2018年第3期5卷 242-250页

作者： Xue-Juan Bai You-Rong Yang Jian-Qin Liang Hui-Ru An Jie Wang Yan-Bo Ling Zhong-Yuan Wang Xue-Qiong Wu Army tuberculosis Prevention and Control Key Laboratory Beijing Key Laboratory of New Techniques of Tuberculosis Diagnosis and Treatment Institute of Tuberculosis Research 309 Hospital of Chinese PLA tuberculosis Department No.2.Institute of tuberculosis Research 309 hospital of Chinese PLA tuberculosis Department No.1.Institute of tuberculosis Research 309 hospital of Chinese PLA tuberculosis Department No.3 Institute of Tuberculosis Research 309 Hospital of Chinese PLA

Background: The diagnosis of bacterium-negative pulmonary tuberculosis(TB) and extra-pulmonary TB is challenging clinically. The detection of the anti-TB antibody has an important, auxiliary, clinical diagnostic value. Therefore, TB antibody detection kits should be screened and evaluated, and the reagents with the highest sensitivity and specificity should be chosen and used ***: The diagnostic performance of 7 commercially available TB antibody detection kits(kits A, B, C, D, E, F and G) based on the gold immunoassay detection of immunoglobulin(Ig) G or IgM antibodies were simultaneously evaluated and compared in 62 TB cases and 56 non-TB cases in a laboratory. A retrospective analysis including 2549 cases was carried out to assess the clinical diagnosis values of bacteriological examinations and TB antibody tests(kits B and H used in the clinic).Results: The sensitivities of TB antibody kits A, B, C, D, E, F and G in the sera from 62 TB patients were 50.0%, 83.9%, 38.7%, 9.7%, 48.4%, 69.4% and 79.0%, respectively; the sensitivities in the sera from 24 smear-negative TB patients were 29.2%, 79.2%, 29.2%, 12.5%, 29.2%, 54.2% and 79.2%, respectively; the specificities in the sera from 56 nonTB patients were 73.2%, 25.0%, 85.7%, 96.4%, 78.6%, 78.6% and 50.0%, respectively. Of the 2549 clinically diagnosed cases, there were 1752 pulmonary TB cases, 505 extra-pulmonary TB cases, 87 old pulmonary TB cases and 205 non-TB cases. The positive results for smear, culture, TB antibody kit B and kit H in pulmonary TB cases were 39.8%(543/1365), 48.6%(372/765), 45.8%(802/1752) and 25.2%(442/1752), respectively; the results in extra-pulmonary TB cases were 3.4%(6/178), 5.8%(4/69), 35.4%(179/505), and 11.3%(57/505), respectively; the results in old pulmonary TB cases were 0%(0/64), 0%(0/30), 32.2%(28/87), and 9.2%(8/87), respectively; and the results in non-TB cases were 0%(0/121), 0%(0/56), 21.5%(44/205), and 2.4%(5/205), respectively. Of 624 smear-positive and/or

关键词： tuberculosis tuberculosis antibody test Smear Culture

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Multichannel Financing Reduces Economic Burden and Improves the Medical Security Level for tuberculosis Patients

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China CDC weekly 2020年第12期2卷 194-197页

作者： Fei Huang Ni Wang You Li Hui Zhang Yanli Yuan Xiaomeng Wang Xiaolin Wang Shitong Huan Lixia Wang Mingting Chen Yanlin Zhao National Center for tuberculosis Control and Prevention China Center for Disease Control and PreventionBeijingChina Jilin Provincial Center for tuberculosis Control and Prevention ChangchunJilinChina Zhejiang Provincial Center for tuberculosis Control and Prevention HangzhouZhejiangChina Ningxia Hui Autonomous Region tuberculosis Dispensary YinchuanNingxia Hui Autonomous RegionChina Bill&Melinda Gates foundation Beijing officeBeijingChina

tuberculosis(TB)is one of the main infectious diseases to simultaneously cause poverty and be caused by *** the economic burden of TB patients in China is essential to improving patient compliance and reducing the impact of the TB *** accordance with the national reform of the medical and health system and the current status of the country’s TB prevention and treatment service system,representatives of the government proposed the concept of a multichannel financing mechanism.

关键词： prevention tuberculosis diseases

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Effects of Mycobacterium vaccae vaccine in a mouse model of tuberculosis: protective action and differentially expressed genes

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Military Medical Research 2020年第2期7卷 125-139页

作者： Wen-Ping Gong Yan Liang Yan-Bo Ling Jun-Xian Zhang You-Rong Yang Lan Wang Jie Wang Ying-Chang Shi Xue-Qiong Wu Army tuberculosis Prevention and Control Key Laboratory/Beijing Key Laboratory of New Techniques of tuberculosis Diagnosis and Treatment Institute for Tuberculosis Researchthe 8th Medical Center of Chinese PLA General HospitalBeijing 100091China

Background:tuberculosis is a leading cause of death *** is an effective vaccine,but not widely used in many parts of the world due to a variety of *** vaccae(***)is another vaccine used in human subjects to prevent *** the current study,we investigated the potential mechanisms of *** vaccination by determining differentially expressed genes in mice infected with *** before and after *** ***:Three days after exposure to *** H37 Rv strain(5×10~5 CFU),adult BALB/c mice randomly received either *** vaccine(22.5μg)or vehicle via intramuscular injection(n=8).Booster immunization was conducted 14 and 28 days after the primary *** expressed genes were identified by microarray followed by standard bioinformatics ***:*** vaccination provided protection against *** infection(most prominent in the lungs).We identified 2,326 upregulated and 2,221 downregulated genes in vaccinated *** changes could be mapped to a total of 123 signaling pathways(68 upregulated and 55 downregulated).Further analysis pinpointed to the MyD88-dependent TLR signaling pathway and PI3 K-Akt signaling pathway as most likely to be ***:*** vaccine provided good protection in mice against *** infection,via a highly complex set of molecular *** findings may provide clue to guide development of more effective vaccine against tuberculosis.

关键词： Mycobacterium tuberculosis Immunotherapeutic effect Immunotherapy Vaccae vaccine Differentially expressed genes Signaling pathway

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Evaluation of IFIT3 and ORM1 as Biomarkers for Discriminating Active tuberculosis from Latent Infection

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Current Medical Science 2022年第6期42卷 1201-1212页

作者： Bing-fen YANG Fei ZHAI Shan YU Hong-juan AN Zhi-hong CAO Yan-hua LIU Ruo WANG Xiao-xing CHENG tuberculosis Prevention and Control Key Laboratory Beijing Key Laboratory of New Techniques of Tuberculosis Diagnosis and TreatmentInstitute of Senior Department of Tuberculosisthe Eighth Medical Center of Chinese PLA General HospitalBeijing100091China Fourth Division Senior Department of Tuberculosisthe Eighth Medical Center of PLA General HospitalBeijing100091China Department of tuberculosis Prevention and Control Senior Department of Tuberculosisthe Eighth Medical Center of PLA General HospitalBeijing100091China

Objective Current commercially available immunological tests cannot be used for discriminating active tuberculosis(TB)from latent TB *** evaluate the value of biomarker candidates in the diagnosis of active TB,this study aimed to identify differentially expressed genes in peripheral blood mononuclear cells(PBMCs)between patients with active TB and individuals with latent TB infection by transcriptome *** The differentially expressed genes in unstimulated PBMCs and in Mycobacterium tuberculosis(Mtb)antigen-stimulated PBMCs from patients with active TB and individuals with latent TB infection were identified by transcriptome *** candidate genes were evaluated in cohorts consisting of 110 patients with TB,30 individuals with latent TB infections,and 50 healthy controls by quantitative real-time *** operating characteristic(ROC)curve analysis was performed to calculate the diagnostic value of the biomarker *** Among the differentially expressed genes in PBMCs without Mtb antigen stimulation,interferon-induced protein with tetratricopeptide repeats 3(IFIT3)had the highest area under curve(AUC)value(0.918,95%CI:0.852-0.984,P<0.0001)in discriminating patients with active TB from individuals with latent TB infection,with a sensitivity of 91.86%and a specificity of 84.00%.In Mtb antigen-stimulated PBMCs,orosomucoid 1(ORM1)had a high AUC value(0.833,95%CI:0.752-0.915,P<0.0001),with a sensitivity of 81.94%and a specificity of 70.00%.Conclusion IFIT3 and ORM1 might be potential biomarkers for discriminating active TB from latent TB infection.

关键词： tuberculosis biomarker latent tuberculosis infection interferon-induced protein with tetratricopeptide repeats 3 orosomucoid 1

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Mesenchymal-epithelial Transition Factor Regulates Monocyte Function during Mycobacterial Infection via Indoleamine 2,3-dioxygenase

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Current Medical Science 2022年第2期42卷 407-416页

作者： Bing-fen YANG Fei ZHAI Hong-juan AN Jing JIANG Zhi-hong CAO Yan-hua LIU Jin-wen SU Ruo WANG Xiao-xing CHENG tuberculosis Prevention and Control Key Laboratory Beijing Key Laboratory of New Techniques of Tuberculosis Diagnosis and TreatmentSenior Department of Tuberculosisthe Eighth Medical Center of Chinese PLA General HospitalBeijing100091China

Objective Mycobacterium tuberculosis(Mtb),the causative agent of tuberculosis(TB),causes an estimated 1.6 million human deaths annually,but the pathogenesis of TB remains *** plays a critical role in the onset and outcome of *** study aimed to uncover the roles of innate and adaptive immunity in *** The gene expression profiles generated by RNA sequencing from human peripheral blood mononuclear cells(PBMCs)stimulated with or without Mtb strain H37Rv antigens were analyzed.A total of 973 differentially expressed mRNAs were *** The differentially expressed genes were enriched in innate immunity signaling *** mesenchymal-epithelial transition factor(MET)gene was significantly upregulated in CD14^(+)monocytes.A MET inhibitor improved the uptake of the BCG strain by monocytes and macrophages as well as inhibited the expression of indoleamine 2,3-dioxygenase(IDO).The expression of IDO was increased in PBMCs stimulated with Mtb antigens,and the IDO inhibitor promoted the expression of CD40,CD83,and *** Our results might provide clues regarding the immunomodulatory mechanisms used by Mtb to evade the host defense system.

关键词： monocytes mycobacteria mesenchymal-epithelial transition factor indoleamine 2,3-dioxygenase

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Effectiveness of the Integrated TB Surveillance System--China,2018-2019

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China CDC weekly 2020年第12期2卷 190-193页

作者： Ni Wang Tao Li Xin Du You Li Miaomiao Sun Shitong Huan Hui Zhang Lixia Wang Mingting Chen Fei Huang Yanlin Zhao National Center for tuberculosis Control and Prevention Chinese Center for Disease Control and PreventionBeijingChina PATH Beijing officeBeijingChina Bill&Melinda Gates foundation Beijing officeBeijingChina

What is already known about this topic?China’s national health information system provides important support and means for deepening the country’s medical and health reform,for improving relevant delivery services,for enhancing the level of scientific management of health,and for promoting the goal of basic medical and health services for everyone in *** is added by this report?To further the construction of the national health information system,the National Center for tuberculosis Control and Prevention of China CDC,started a pilot project for a new tuberculosis(TB)integrated health(iHealth)surveillance system,which was integrated with regional health information *** goal was to explore automatic data exchange between hospitals and disease control facilities to reduce the workload of *** are the implications for public health practice?This pilot proved that data sharing and automatic exchanges between different information systems can be achieved through a unified surveillance dataset,which could provide a reference point for the construction of surveillance systems for other infectious diseases or for the entire public health information system.

关键词： services deepening everyone

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多阶段结核菌素调查设计:关于抽样的一些建议

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国际结核病与肺部疾病杂志 2000年第4期 101-107页

作者： N.J.D.Nagelkerke M.W.Borgdorff N.A.Kalisvaart J.F.Broekmans 王海东 Royal Netherlands tuberculosis Association(kncv) The Hague The Netherlands

背景:用儿童的结核菌素调查评价国家或地区的感染率时通常应用多阶段调查设计。这些调查需要在每个阶段的亚单位采用有效的抽样方法。目的:制定结核菌素调查抽样的指南。设计:应用抽样理论制定一个简单而有效的抽样方法来计划和分析结... 详细信息

背景:用儿童的结核菌素调查评价国家或地区的感染率时通常应用多阶段调查设计。这些调查需要在每个阶段的亚单位采用有效的抽样方法。目的:制定结核菌素调查抽样的指南。设计:应用抽样理论制定一个简单而有效的抽样方法来计划和分析结核菌素调查。样本大小的问题应予考虑。结果:制定了感染率和其可信区间的计算公式。讨论了样本大小。结论:我们建议用样本比例大小的抽样方法抽取区,这样有较大可能性将较大的单位包括在样本中。一个区内的每个学校有同样的可能性被包括在样本中,则采用单纯随机抽样方法进行学校抽样。每个学校所有符合条件的儿童均做皮内(Mantoux)试验。每个区试验的儿童人数应大致恒定,增加抽取区的数量要比每个区增加几百到几千儿童的数量更有效的提高估算的准确性。

关键词：结核菌素调查抽样

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越南的家庭支出与结核病患病率:通过一套家庭指标进行预测

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国际结核病与肺部疾病杂志 2011年第4期6卷 174-179页

作者： N.B.Hoa E.W.Tiemersma D.N.Sy N.V.Nhung A.Gebhard M.W.Borgdorff F.G.J.Cobe-lens 彭红 National tuberculosis Programme Hanoi Viet Nam Research Unit KNCV Tuberculosis Foundation The Hague Center for Poverty-Related Communicable Diseases Amsterdam Institute of Global Health and Development Academic Medical Center Amsterdam International Unit KNCV Tuberculosis Foundation The Hague Department of Clinical Epidemiology Biostatistics and Bioinformatics Academic Medical Center University of Amsterdam Amsterdam The Netherlands

目的:使用9项家庭特征,在越南全国结核病患病率调查过程研究结核病与家庭支出之间的关系。方法:为评估越南的结核病患病情况,于2006—2007年开展了全国分层整群抽样调查。在第二次越南生活水平调查中采用的9项家庭特征均以户为单位给予... 详细信息

目的:使用9项家庭特征,在越南全国结核病患病率调查过程研究结核病与家庭支出之间的关系。方法:为评估越南的结核病患病情况,于2006—2007年开展了全国分层整群抽样调查。在第二次越南生活水平调查中采用的9项家庭特征均以户为单位给予了评分。在越南生活水平调查数据库中,我们对这9项特征按人均家庭支出进行了回归分析;在我们的调查中,以系数来预测家庭支出水平(5分位级别),并评估其与结核病患病率的关系。结果:15岁以上人群的细菌学确诊结核病患病率为307/10万(95%可信区间为249～366)。经过对干扰因素的调整,发现结核病患病率与家庭支出水平相关:五分位为最低家庭支出水平人群的患病率比五分位为最高家庭支出水平人群高2.5倍(95%可信区间为1.6～3.9)。结论:通过9项家庭特征,我们能够非常准确地预测家庭支出水平。结核病患病率与估算的家庭支出水平之间存在显著相关性,提示在越南结核病与贫困相关。

关键词：越南患病率社会-经济结核病

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Investigation of the cell composition and gene expression in the delayed-type hypersensitivity tuberculin skin test

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Military Medical Research 2024年第1期11卷 148-151页

作者： Hui-Juan Duan Hong-Qian Chu Ting-Ming Cao Guang-Ming Dai Na Tian Gang Sheng Zhao-Gang Sun Beijing Key Laboratory in Drug Resistant tuberculosis Research Beijing Tuberculosis and Thoracic Tumor Research InstituteBeijing Chest HospitalCapital Medical UniversityBeijing 101149China

Dear Editor,The tuberculin skin test(TST)reagents have continuously improved,with the ESAT6-CFP10(EC)test having recently been introduced,but are seldom based on the direction of the delayed-type hypersensitivity(DTH)*** studies only partially showed the infiltration and activation of immune cells and the production of cytokines of the skin induration[1,2],and lack the detailed measurements of cell proportions and gene expression in the DTH ***,in this study,we revealed the comprehensive characteristics of DTH by single-cell RNA sequencing(scRNA-seq)in the guinea pig tuberculosis(TB)model[Experimental Animal Welfare Ethics Committee,Beijing tuberculosis and Thoracic Tumor Research Institute(2021-064)].

关键词： Tuberculin skin test Delayed-type hypersensitivity Single-cell RNA sequencing

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Activities of Biapenem against Mycobacterium tuberculosis in Macrophages and Mice

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Biomedical and Environmental Sciences 2019年第4期32卷 235-241页

作者： GUO Zhen Yong ZHAO Wei Jie ZHENG Mei Qin LIU Shuo YAN Chen Xia LI Peng XU Shao Fa Beijing Chest Hospital affiliated with the Capital Medical University/Beijing tuberculosis and Thoracic Tumor Institute

Objective To assess the activities of biapenem against multidrug-resistant and extensively drug-resistant Mycobacterium tuberculosis. Methods Biapenem/clavulanate(BP/CL) was evaluated for in vitro activity against Mycobacterium tuberculosis(Mtb) multidrug-resistant(MDR) isolates, extensively drug-resistant(XDR) isolates, and the H37 RV strain. BP/CL activity against the H37 Rv strain was assessed in liquid cultures, in macrophages, and in mice. Results BP/CL exhibited activity against MDR and XDR Mtb isolates in liquid cultures. BP/CL treatment significantly reduced the number of colony forming units(CFU) of Mtb within macrophages compared with control untreated infected macrophages. Notably, BP/CL synergized in pairwise combinations with protionamide, aminosalicylate, and capreomycin to achieve a fractional inhibitory concentration for each pairing of 0.375 in vitro. In a mouse tuberculosis infection model, the efficacy of a cocktail of levofloxacin + pyrazinamide + protionamide + aminosalicylate against Mtb increased when the cocktail was combined with BP/CL, achieving efficacy similar to that of the positive control treatment(isoniazid + rifampin + pyrazinamide) after 2 months of treatment. Conclusion BP/CL may provide a new option to clinically treat MDR tuberculosis.

关键词： Biapenem Clavulanate Multidrug resistant Extensive drug-resistant Mycobacterium tuberculosis Activity Macrophage Synergy

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