Effects of Mycobacterium vaccae vaccine in a mouse model of tuberculosis: protective action and differentially expressed genes

作     者：Wen-Ping Gong Yan Liang Yan-Bo Ling Jun-Xian Zhang You-Rong Yang Lan Wang Jie Wang Ying-Chang Shi Xue-Qiong Wu Wen-Ping Gong;Yan Liang;Yan-Bo Ling;Jun-Xian Zhang;You-Rong Yang;Lan Wang;Jie Wang;Ying-Chang Shi;Xue-Qiong Wu

作者机构：Army Tuberculosis Prevention and Control Key Laboratory/Beijing Key Laboratory of New Techniques of Tuberculosis Diagnosis and TreatmentInstitute for Tuberculosis Researchthe 8th Medical Center of Chinese PLA General HospitalBeijing 100091China 

出 版 物：《Military Medical Research》 (军事医学研究（英文版）)

年 卷 期：2020年第7卷第2期

页      面：125-139页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：supported by Grants from the National Natural Science Foundation of China(81801643) the National Key Program for Infectious Disease of China(2018ZX10731301–005) Beijing Municipal Science&Technology Commission(Z181100001718005) the Medical Science and Technology Youth Cultivation Program of PLA(16QNP075) 

主　　题：Mycobacterium tuberculosis Immunotherapeutic effect Immunotherapy Vaccae vaccine Differentially expressed genes Signaling pathway 

摘      要：Background:Tuberculosis is a leading cause of death *** is an effective vaccine,but not widely used in many parts of the world due to a variety of *** vaccae(***)is another vaccine used in human subjects to prevent *** the current study,we investigated the potential mechanisms of *** vaccination by determining differentially expressed genes in mice infected with *** before and after *** ***:Three days after exposure to *** H37 Rv strain(5×10~5 CFU),adult BALB/c mice randomly received either *** vaccine(22.5μg)or vehicle via intramuscular injection(n=8).Booster immunization was conducted 14 and 28 days after the primary *** expressed genes were identified by microarray followed by standard bioinformatics ***:*** vaccination provided protection against *** infection(most prominent in the lungs).We identified 2,326 upregulated and 2,221 downregulated genes in vaccinated *** changes could be mapped to a total of 123 signaling pathways(68 upregulated and 55 downregulated).Further analysis pinpointed to the MyD88-dependent TLR signaling pathway and PI3 K-Akt signaling pathway as most likely to be ***:*** vaccine provided good protection in mice against *** infection,via a highly complex set of molecular *** findings may provide clue to guide development of more effective vaccine against tuberculosis.