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detection of KRAS G12d in colorectal cancer stool by droplet digital PCR

World Journal of Gastroenterology 2017年第39期23卷 7087-7097页

作者： Susana Olmedillas-López dennis César Lévano-Linares Carmen Laura Aúz Alexandre Luz Vega-Clemente Edurne León Sánchez Alejandro Villagrasa Jaime Ruíz-Tovar Mariano García-Arranz damián García-Olmo foundation Health Research Institute-Fundación jiménez díaz University Hospital department of Surgery School of Medicine Universidad Autónoma de Madrid department of Surgery Rey Juan Carlos University Hospital department of Pathology Fundación Jiménez Díaz University Hospital department of Biomedicine and Biotechnology Universidad de Alcalá department of Surgery Fundación Jiménez Díaz University Hospital

AIMTo assess KRAS G12d mutation detection by droplet digital PCR (ddPCR) in stool-derived dNA from colorectal cancer (CRC) *** this study, tumor tissue and stool samples were collected from 70 patients with stage I-IV CRC diagnosed by preoperative biopsy. KRAS mutational status was determined by pyrosequencing analysis of dNA obtained from formalin-fixed paraffin-embedded (FFPE) tumor tissues. The KRAS G12d mutation was then analyzed by ddPCR in FFPE tumors and stool-derived dNA from patients with this point mutation. Wild-type (WT) tumors, as determined by pyrosequencing, were included as controls; analysis of FFPE tissue and stool-derived dNA by ddPCR was performed for these patients as *** the total 70 patients included, KRAS mutations were detected by pyrosequencing in 32 (45.71%), whereas 38 (54.29%) had WT tumors. The frequency of KRAS mutations was higher in left-sided tumors (11 located in the right colon, 15 in the left, and 6 in the rectum). The predominant point mutation was KRAS G12d (14.29%, n = 10), which was more frequent in early-stage tumors (I-IIA, n = 7). In agreement with pyrosequencing results, the KRAS G12d mutation was detected by ddPCR in FFPE tumor-derived dNA, and only a residual number of mutated copies was found in WT controls. The KRAS G12d mutation was also detected in stool-derived dNA in 80% of all fecal samples from CRC patients with this point *** is a reliable and sensitive method to analyze KRAS G12d mutation in stool-derived dNA from CRC patients, especially at early stages. This non-invasive approach is potentially applicable to other relevant biomarkers for CRC management.

关键词： droplet digital PCR KRAS Stool Formalin-fixed paraffin-embedded Pyrosequencing Colorectal cancer

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Antisense oligonucleotides provide optimism to the therapeutic landscape for tauopathies

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Neural Regeneration Research 2025年第3期20卷 803-804页

作者： Glenn A.Harris Lauren R.Hirschfeld Rainwater Charitable foundation Fort WorthTXUSA

Tauopathies are a group of neurodegenerative diseases characterized by abnormal metabolism of the misfolded tau protein. Tau is encoded by the microtubule-associated protein tau gene(MAPT) and can be classified as either 3-repeat(3R) or 4-repeat(4R) based on the number of repeat domains from alternative splicing of exon 10 of the MAPT gene.

关键词： tau metabolism diseases

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New evidence for a role of Bisphenol A in cell *** in the human population

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BIOCELL 2022年第2期46卷 305-308页

作者： RAFAEL MORENO-GÓMEZ-TOLEdANO MARÍA IARENAS ESPERANZA VÉLEZ-VÉLEZ RICARdO J.BOSCH Universidad de Alcalá Laboratory of Renal Physiology and Experimental NephrologyDepartment of Biological Systems/Physiology28871Alcaláde HenaresSpain Universidad de Alcalá Cell Biology UnitDepartment of Biomedicine and Biotechnology28871Alcaláde HenaresSpain jiménez díaz foundation Fundación Jiménez Díaz School of NursingMadrid28040Spain

Bisphenol A(BPA)is a xenoestrogen known for its implications for the endocrine systems and several other organs,including the *** renal studies have shown that BPA can induce alterations of the cytoskeleton and cell adhesion mechanisms such as a podocytopathy with proteinuria and hypertension,alterations involved in the progression of renal *** data and the fact that BPA is known to be present in the urine of almost the entire population strongly suggest the critical need to reevaluate BPA exposures considered safe.

关键词： Bisphenol A Podocyte Cytoskeleton Cell Adhesion Chronic Renal diseases dialysis

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Association between urinary concentrations of bisphenol A substitutes and diabetes in adults

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World Journal of diabetes 2022年第7期13卷 521-531页

作者： Rafael Moreno-Gómez-Toledano Esperanza Vélez-Vélez María I Arenas Marta Saura Ricardo J Bosch Universidad de Alcalá Department of Biological Systems/Physiology UnitAlcaláde Henares 28871Spain Fundación jiménez díaz School of Nursing Jiménez Díaz FoundationAutonomous University of MadridMadrid 28040Spain Universidad de Alcalá Department of Biomedicine and BiotechnologyAlcaláde Henares 28871Spain Centro de Investigación en Red de Enfermedades Cardiovasculares Instituto Ramón y Cajal de Investigación SanitariaMadrid 28034Spain

BACKGROUNd due to new restrictions on the use of bisphenol A(BPA),industries are beginning to replace it with derived molecules such as bisphenol S and F(BPS and BPF).There is extensive evidence in the academic literature on the potential health effects of BPA,which is known to be a diabetogenic ***,there are few publications related to new compounds derived from *** To perform an epidemiological study of urinary BPS and BPF in the American National Health and Nutrition Examination Survey(NHANES)cohort,and analyze their possible relationship with diabetes *** NHANES datasets from 2013 to 2016 were used due to the urinary BPF and BPS *** from 3658 adults were analyzed to perform regression analysis exploring the possible relationship between BPA-derived compounds and *** descriptive statistics,linear regression modeling,and logistic regression analysis revealed a significant relationship between urinary BPS,but not BPF,and diabetes ***,a relationship was observed between both compounds and hypertension and a slight relationship between BPF and *** In the present study,a strong relationship between urinary BPS,not BPF,and diabetes risk has been *** substitute molecules do not exempt the population from potential health risks.

关键词： Bisphenol S Bisphenol F diabetes mellitus National Health and Nutrition Examination Survey Urine

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Human endogenous retrovirus type-W and multiple sclerosis–related smoldering neuroinflammation

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Neural Regeneration Research 2025年第3期20卷 813-814页

作者： Joel Gruchot Laura Reiche Andrew Chan Robert Hoepner Patrick Küry department of Neurology Medical Faculty and University Hospital DüsseldorfHeinrich Heine University DüsseldorfDüsseldorfGermany department of Neurology InselspitalBern University Hospital and University of BernBernSwitzerland

Introduction to human endogenous retrovirus type-W(HERV-W): Genomic inheritance from the past includes retroviral sequences that have been stably incorporated into our genomes and account for up to 8% of human dNA.

关键词： endogenous inflammation Human

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Adding new dimension to non-directed C（sp3）–H functionalizations

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Science China Chemistry 2025年

作者： Konstantin P.Bryliakov Laboratory of Selective Oxidation Catalysis N.D.Zelinsky Institute of Organic Chemistry RAS

Aliphatic C–H groups are among the most abundant yet least reactive functional groups in organic molecules. Nature has evolved metalloenzymes such as those of the cytochrome P450 superfamily that efficiently activate individual tertiary（3） and secondary （2） C（sp3）–H bonds in complex molecules with the highest level of regio-and stereoselectivity [1], thus encouraging studies aimed at engineering artificial metalloenzymes for synthetic applications [2].

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Working Out What Works:The Case of the Education Endowment foundation in England

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ECNU Review of Education 2021年第1期4卷 46-64页

作者： Triin Edovald Camilla Nevill The Education Endowment foundation

Purpose:This article gives an overview of the successes and lessons learned to date of the Education Endowment foundation(EEF),one of the leading organizations of the What Works movement design/Approach/Methods:Starting with its history,this article covers salient components of the EEF's unique journey including lessons learned and challenges in evidence ***:The EEF has demonstrated that it is feasible to rapidly expand the use of school-based randomized controlled trials(RCTs)in a country context,set high standards for research independence,transparency,and design,and generate new evidence on what *** include the need to consider alternative designs to RCTs to answer a range of practice-relevant questions,how to best test interventions at scale,and how study findings are reported and ***/Value:This article addresses some of the key components required for the success of What Works organizations globally.

关键词： Education evaluation evidence impact randomized controlled trials teaching

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Locally Enhanced Flow and Electric Fields Through a Tip Effect for Efficient Flow‑Electrode Capacitive deionization

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Nano-Micro Letters 2025年第2期17卷 1-17页

作者： Ziquan Wang Xiangfeng Chen Yuan Zhang Jie Ma Zhiqun Lin Amor Abdelkader Maria‑Magdalena Titirici Libo deng College of Chemistry and Environmental Engineering Shenzhen UniversityShenzhen 518060People’s Republic of China Research Center for Environmental Functional Materials College of Environmental Science and EngineeringTongji University1239 Siping RoadShanghai 200092People’s Republic of China department of Chemical and Biomolecular Engineering National University of SingaporeSingapore 117585Singapore department of Engineering Faculty of Science and TechnologyBournemouth UniversityTalbot CampusFern BarrowPooleEngland BH125BBUK Institut de Chimie de Nice UniversitéCôte d’AzurUMR CNRS 727228 Av.Valrose06108 NiceFrance department of Chemical Engineering Imperial College LondonSouth Kensington CampusExhibition RdLondon SW72AZUK

Low-electrode capacitive deionization(FCdI)is an emerging desalination technology with great potential for removal and/or recycling ions from a range of ***,it still suffers from inefficient charge transfer and ion transport kinetics due to weak turbulence and low electric intensity in flow electrodes,both restricted by the current ***,a new tip-array current collector(designated as T-CC)was developed to replace the conventional planar current collectors,which intensifies both the charge transfer and ion transport *** effects of tip arrays on flow and electric fields were studied by both computational simulations and electrochemical impedance spectroscopy,which revealed the reduction of ion transport barrier,charge transport barrier and internal *** the voltage increased from 1.0 to 1.5 and 2.0 V,the T-CC-based FCdI system(T-FCdI)exhibited average salt removal rates(ASRR)of 0.18,0.50,and 0.89μmol cm^(-2) min^(-1),respectively,which are 1.82,2.65,and 2.48 folds higher than that of the conventional serpentine current collectors,and 1.48,1.67,and 1.49 folds higher than that of the planar current ***,with the solid content in flow electrodes increased from 1 to 5 wt%,the ASRR for T-FCdI increased from 0.29 to 0.50μmol cm^(-2) min^(-1),which are 1.70 and 1.67 folds higher than that of the planar current ***,a salt removal efficiency of 99.89%was achieved with T-FCdI and the charge efficiency remained above 95%after 24 h of operation,thus showing its superior long-term stability.

关键词： Flow-electrode Capacitive deionization Current collector Tip effect desalination

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How dopamine tunes parvalbumin interneurons in the hippocampus:new experimental observations in Alzheimer's disease

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Neural Regeneration Research 2025年第5期20卷 1405-1406页

作者： Livia La Barbera Paraskevi Krashia Annalisa Nobili department of Medicine and Surgery UniversitàCampus Bio-Medico di RomaViaÁlvaro del PortilloRomeItaly department of Experimental Neurosciences IRCCS Santa Lucia FoundationVia del Fosso di FioranoRomeItaly department of Science and Technology for Sustainable development and One Health UniversitàCampus Bio-Medico di RomaViaÁlvaro del PortilloRomeItaly

despite decades of dedicated resea rch,Alzheimer's disease (Ad) is an age-related and progressive neurodegenerative disorder for which the mechanisms of onset are sti unc *** is cha racterized by featured histological alterations including amyloid-beta (AB) plaque deposition,accumulation of neurofibrillary to ngles of hyperphosphorylated-tau,and neuronal loss,accompanied by progressive cognitive decline and behavioral changes.

关键词： Alzheimer alterations dopamine

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Sex differences in aging and injured brain

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Neural Regeneration Research 2025年第10期20卷 2901-2902页

作者： Jordan N.Williamson Yuan Yang University of Illinois Urbana-Champaign Grainger College of EngineeringDepartment of BioengineeringUrbanaILUSA Carle foundation Hospital Stephenson Family Clinical Research InstituteClinical Imaging Research CenterUrbanaILUSA University of Illinois Urbana-Champaign Beckman Institute for Advanced Science and TechnologyUrbanaILUSA Northwestern University Department of Physical Therapy and Human Movement SciencesChicagoILUSA

Background:The prevalence,age of onset,and symptomatology of traumatic brain injury,stroke,and neurodegenerative diseases(such as Alzheimer’s disease(Ad),Parkinson’s disease,amyotrophic lateral sclerosis,and Huntington’s disease)differ substantially between males and *** higher prevalence of these brain disorders has been attributed to females having a greater longevity compared with *** one of the greatest risk factors of acquired brain injury(such as stroke,traumatic brain injury caused by fall)and neurodegenerative disease is age.

关键词： Huntington prevalence traumatic

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