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AAV2-PDE6B restores retinal structure and function in the retinal degeneration 10 mouse model of retinitis pigmentosa by promoting phototransduction and inhibiting apoptosis

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Neural Regeneration Research 2025年第8期20卷 2408-2419页

作者： Ruiqi Qiu Mingzhu Yang Xiuxiu Jin Jingyang Liu Weiping Wang Xiaoli Zhang Jinfeng Han Bo Lei Henan Eye Hospital Henan Provincial People’s HospitalPeople’s Hospital of Zhengzhou UniversityZhengzhouHenan ProvinceChina Eye institute Henan Academy of Innovations in Medical ScienceZhengzhouHenan ProvinceChina Branch of National Clinical Research Center for Ocular disease Henan Provincial People’s HospitalZhengzhouHenan ProvinceChina Academy of Medical Science Zhengzhou UniversityZhengzhouHenan ProvinceChina

Retinitis pigmentosa is a group of inherited diseases that lead to retinal degeneration and photoreceptor cell ***,there is no effective treatment for retinitis pigmentosa caused by PDE6B ***-associated virus(AAV)-mediated gene therapy is a promising strategy for treating retinitis *** aim of this study was to explore the molecular mechanisms by which AAV2-PDE6B rescues retinal *** do this,we injected retinal degeneration 10(rd10)mice subretinally with AAV2-PDE6B and assessed the therapeutic effects on retinal function and structure using dark-and light-adapted electroretinogram,optical coherence tomography,and ***-independent acquisition-mass spectrometry-based proteomic analysis was conducted to investigate protein expression levels and pathway enrichment,and the results from this analysis were verified by real-time polymerase chain reaction and western ***2-PDE6B injection significantly upregulated PDE6βexpression,preserved electroretinogram responses,and preserved outer nuclear layer thickness in rd10 *** expressed proteins between wild-type and rd10 mice were closely related to visual perception,and treating rd10 mice with AAV2-PDE6B restored differentially expressed protein expression to levels similar to those seen in wild-type *** Encyclopedia of Genes and Genome analysis showed that the differentially expressed proteins whose expression was most significantly altered by AAV2-PDE6B injection were enriched in phototransduction ***,the phototransductionrelated proteins Pde6α,Rom1,Rho,Aldh1a1,and Rbp1 exhibited opposite expression patterns in rd10 mice with or without AAV2-PDE6B ***,Bax/Bcl-2,p-ERK/ERK,and p-c-Fos/c-Fos expression levels decreased in rd10 mice following AAV2-PDE6B *** data suggest that AAV2-PDE6B-mediated gene therapy promotes phototransduction and inhibits apoptosis by inhibiting the ERK signaling pathway and upr

关键词： apoptosis AAV2-PDE6B ERK1/2 gene therapy phototransduction proteomics rd10 retinitis pigmentosa

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Increased excitatory amino acid transporter 2 levels in basolateral amygdala astrocytes mediate chronic stress–induced anxiety-like behavior

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Neural Regeneration Research 2025年第6期20卷 1721-1734页

作者： Xirong Xu Shoumin Xuan Shuai Chen Dan Liu Qian Xiao Jie Tu Shenzhen Key Laboratory of Neuroimmunomodulation for Neurological diseases Shenzhen-Hong Kong Institute of Brain ScienceShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina CAS Key Laboratory of Brain Connectome and Manipulation Brain Cognition and Brain Disease Institute(BCBDI)Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina Guangdong provincial Key Laboratory of Brain Connectome and Behavior the Brain Cognition and Brain Disease Institute(BCBDI)Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina University of Chinese of Academy of Sciences BeijingChina Faculty of Life and Health Sciences Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina

The conventional perception of astrocytes as mere supportive cells within the brain has recently been called into question by empirical evidence, which has revealed their active involvement in regulating brain function and encoding behaviors associated with ***, astrocytes in the basolateral amygdala have been found to play a role in the modulation of anxiety-like behaviors triggered by chronic stress. Nevertheless, the precise molecular mechanisms by which basolateral amygdala astrocytes regulate chronic stress–induced anxiety-like behaviors remain to be fully elucidated. In this study, we found that in a mouse model of anxiety triggered by unpredictable chronic mild stress, the expression of excitatory amino acid transporter 2 was upregulated in the basolateral amygdala. Interestingly, our findings indicate that the targeted knockdown of excitatory amino acid transporter 2 specifically within the basolateral amygdala astrocytes was able to rescue the anxiety-like behavior in mice subjected to stress. Furthermore, we found that the overexpression of excitatory amino acid transporter 2 in the basolateral amygdala, whether achieved through intracranial administration of excitatory amino acid transporter 2agonists or through injection of excitatory amino acid transporter 2-overexpressing viruses with GfaABC1D promoters, evoked anxiety-like behavior in mice. Our single-nucleus RNA sequencing analysis further confirmed that chronic stress induced an upregulation of excitatory amino acid transporter 2 specifically in astrocytes in the basolateral amygdala. Moreover, through in vivo calcium signal recordings, we found that the frequency of calcium activity in the basolateral amygdala of mice subjected to chronic stress was higher compared with normal *** knocking down the expression of excitatory amino acid transporter 2 in the basolateral amygdala, the frequency of calcium activity was not significantly increased, and anxiety-like behavior was ob

关键词： anxiety astrocytes basolateral amygdala behavior dihydrokainic acid excitatory amino acid transporter 2 fiber photometry glutamate LDN-212320 transporter

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Synthesis,Crystal Structure and Biological Evaluation of 4-Chloro-2-(morpholine-4-carbonyl)phenyl-4′-methoxybenzoate as Molluscicidal Agent

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Chinese Journal of Structural Chemistry 2014年第6期33卷 841-845页

作者：段李平秦志强李广平李石柱李强许东芳 National institute of parasitic diseases Chinese Center for Disease Control and Prevention Hunan institute of parasitic disease Shanghai Normal University

The title compound 4-chloro-2-（morpholine-4-carbonyl）phenyl-4＇-methoxybenzoate（C19H18ClNO5, Mr = 375.79）, a 5-chlorosalicylic acid derivative incorporating morpholine moiety, was conveniently synthesized through two steps and crystallized in the monoclinic space group P21/c with a = 7.8501（10）, b = 9.3149（12）, c = 25.133（3）, V = 1836.2（4）3, Z = 4, Dc = 1.359 Mg/m^3, λ = 0.71073, μ（MoKα） = 0.237 mm^-1, F（000） = 784, R = 0.0447 and wR = 0.1227 for 3982 independent reflections with I 〉 2σ（I）. The dihedral angles formed between 4-methoxybenzene and 5-chlorobenzene rings, the 5-chlorobenzene and morpholine, and 4-methoxybenzene and morpholine rings are 82.55（3）, 72.56（5） and 47.68（0）° by X-ray analysis, respectively. Bioassay shows that the title compound has a good molluscidal effect.

关键词： salicylic acid crystal structure molluscidal

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An NIR-Ⅱ-emissive Ru(Ⅱ) metallacycle for efficient and safe immunotherapy

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Science China Chemistry 2025年第1期 5-7页

作者： Chonglu Li Jianbo Zhan Biao Dong institute of Health Inspection and Testing Hubei Provincial Center for Disease Control and Prevention State Key Laboratory on Integrated Optoelectronics College of Electronic Science and Engineering Jilin University

Immunotherapy, as a groundbreaking cancer treatment, has gained considerable attention for its utilization of the body’s immune system to combat tumors [1]. This therapy induces tumor cell apoptosis, triggering a sustained anti-tumor immune response and effectively preventing tumor recurrence and metastasis [2]. The fundamental principle lies in inducing tumor cell death through specific treatment modalities, leading to the release of a cascade of signaling molecules and antigens, thereby mobilizing the immune system to actively identify and attack tumor cells [3].

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Synthesis, Crystal Structure and Anti-parasitic Activity of 2-(2-Methoxy-4-nitrophenylcarbamoyl)phenyl 4-Fluorobenzoate

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Chinese Journal of Structural Chemistry 2013年第6期32卷 847-852页

作者：段李平吴宁波刘丛珊陶奕张雪强张浩冰 National institute of parasitic diseases Chinese Center for Disease Control and Prevention Key Laboratory of Parasitology and Vector Biology at National Institute of Parasitic Diseases

The title compound 2-（2-methoxy-4-nitrophenylcarbamoyl）phenyl-4＇-fluorobenzoate （C21H15FN206, Mr = 410.35）, a fluorine-containing derivative of salicylamide, was conve- niently synthesized through two steps and crystallized in the orthorhombic space group P21/c with a = 7.6453（15）, b = 14.323（3）, c = 17.035（3） A, V= 1865.4（6） A3, Z = 4, Dc = 1.461 Mg/m^3, 2 = 0.71073 A, μ（MoKa） = 0.115 mm-1, F（000） = 848, R = 0.0705 and wR = 0.1834 for 3267 independent refections with 1 〉 2σ（I）. X-ray analysis reveals that the dihedral angles formed between the 2-methoxy-4-nitrobenzene and benzene ring, the benzene and 4-fluorobenzene, and the 2-methoxy-4-nitrobenzene and 4-fluorobenzene ring are 3.2（4）, 69.8（3） and 72.9（2）~, respectively. Bioassay shows that the title compound has anti-parasitic activity against hydatid protoscoleces.

关键词： 2-(2-methoxy-4-nitrophenyicarbamoyl)phenyl-4'-fluorobenzoate synthesis anti-parasitic crystal structure

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The history of the elimination of lymphatic filariasis in China

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Infectious diseases of Poverty 2013年第1期2卷 249-257页

作者： Sun De-jian Deng Xu-li Duan Ji-hui institute of parasitic diseases Chinese Center for Disease Control and PreventionPO Box 200025ShanghaiChina Shandong institute of parasitic diseases PO Box 272133 JiningChina Hunan provincial Center for disease Control and Prevention PO Box 410005ChangshaChina

China used to be one of the most heavily endemic countries for lymphatic filariasis(LF)in the *** were 864 endemic counties/cities in 16 provinces/autonomous regions/municipalities(P/A/M)with a total population of 330 million at risk of *** the founding of the People’s Republic of China in 1949,the Chinese Government has designated the control of the disease to be a top *** to decades of sustained efforts,close cooperation related to LF control among government departments,and active participation of endemic populations,an all-round campaign for prevention and control has been carried out vigorously and *** many years,great achievements have been made through persistent endeavors of Chinese scientists and disease control *** ultimate goal to eliminate LF in the country was achieved in 2006.

关键词： Lymphatic filariasis Control strategies Elimination

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Malaria Transmission Potential in the Three Gorges Reservoir of the Yangtze River, China

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Biomedical and Environmental Sciences 2013年第1期26卷 54-62页

作者： WANG Duo Quan TANG Lin Hua GU Zhen Cheng ZHENG Xiang JIANG Wei Kang National institute of parasitic disease Chinese Center for Disease Control and Prevention WHO Collaborating Center for Malaria Schistosomiasis and filariasis

Objective To define and evaluate the malaria transmission potential in the Yangtze River, following construction of the Three Gorges Reservoir. Methods Six villages, namely, Kaixian, Fengjie, Wanzhou, Fuling, Yubei, and Zigui were selected for investigating the malaria transmission potential in the reservoir. Transmission potential was estimated by mathematical modeling and evaluation of the local malaria situation. Factors that influenced the transmission potential were analyzed using Poisson regression analysis in combination with Grey Systematic Theory （based on evaluation by the Delphi method）. Results Indirect fluorescent antibody data and the incidence of malaria in the local population were consistent with the malaria transmission potential calculated for the area. Multivariate Poisson regression analysis showed a statistically significant association between the riparian zone and the man‐biting rate. Conclusion The risk of a malaria epidemic can be forecasted using the malaria transmission potential parameters investigated here which was closely correlated with the riparian zone.

关键词： Malaria Transmission potential Yangtze River Three Gorges Reservoir Riparian zone

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Study of Infection of Echinococcus granulosus in Yak in Spring and Its Potential Role in Transmission of Cystic Echinococcosis in Rangtang County of Sichuan,China

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Biomedical and Environmental Sciences 2013年第3期26卷 226-229页

作者： HU Huan Huan WU Wei Ping WANG Li Ying WANG Qian HUANG Yan GUAN Ya Yi National institute of parasitic diseases Chinese Center for Disease Control and Prevention WHO Collaborating Center for Malaria Schistosomiasis and Falariasis Key Laboratory of Parasite and Vector Biology Lab of Exercise Epidemiology Graduate School of Sport SciencesWaseda University institute of parasitic disease Control Sichuan Provincial Center for Disease Control

Dear Editor, Cystic echinococcosis （CE）, caused by Echinococcus granulosus in larval stage, is considered as one of the most dangerous parasitic zoonosis in the world. The obligate 2-host parasitic cycle of Echinococcus granulosus is predominantly synanthropic. Dogs are the usual definitive hosts, and lots of mammalian species can be intermediate hosts, including domestic livestock and human[I2]. In the Tibetan plateau, China, the population is mainly Tibetans primarily engaged in livestock husbandry and CE is therefore a health problem for both people and animal in Tibetan communities. The reported infection rate of Echinococcus gronulosus in slaughtered yak in slaughterhouses is usually very high, being about 50% or higher as reported, and the liver and lungs are the main affected organs[34].

关键词： Study of Infection of Echinococcus granulosus in Yak in Spring and Its Potential Role in Transmission of Cystic Echinococcosis in Rangtang County of Sichuan China

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Malaria Surveillance of Entry People During the COVID-19 Epidemic—Guangdong Province,China,October 2020–May 2021

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China CDC weekly 2021年第38期3卷 799-802页

作者： De Wu Zhuohui Deng Rongxin Lin Qiang Mao Wenchen Lu Caiwen Ruan Yongzhen Cen Ning Xiao Tie Song institute for parasitic disease Control and Prevention Center for Disease Control and Prevention of GuangdongGuangzhouGuangdongChina National institute of parasitic diseases Chinese Center for Disease Control and PreventionShanghaiChina.

Summary What is already known about this topic?Malaria control was affected by the coronavirus disease 2019(COVID-19)*** study conducted active case finding for key flights and key populations to determine malaria *** is added by this report?Surveillance for malaria was conducted for entry personnel coming from areas affected by *** is estimated that at least 100,000 tests were conducted in Guangdong Province.

关键词： Province. Guangdong Malaria

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Seroepidemiological Investigation of Lyme disease and Human Granulocytic Anaplasmosis among People Living in Forest Areas of Eight Provinces in China

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Biomedical and Environmental Sciences 2013年第3期26卷 185-189页

作者： HAO Qin GENG Zhen HOU Xue Xia TIAN Zhen YANG Xiu Jun JIANG Wei Jia SHI Yan ZHAN Zhi Fei LI Guo Hua YU De Shan WANG Hua Yong XU Jian Guo WAN Kang Lin National institute for Communicable disease Control and Prevention Chinese Center for Disease Control and Prevention State Key Laboratory for Infectious disease Prevention and Control Henan Oil Prospecting Bureau Jilin provincial Centre for disease Control and Prevention Guizhou provincial Centre for disease Control and Prevention Qinghai provincial Centre for disease Control and Prevention Hunan provincial Centre for disease Control and Prevention Shanxi provincial Centre for disease Control and Prevention Gansu provincial Centre for disease Control and Prevention Miyun Centre for disease Control and Prevention

Objective Lyme disease and Human granulocytic anaplasmosis are tick-borne diseases caused by Borrelia burgdorferi and Anoplasrna phagocytophilum respectively. We have investigated infection and co-infection of the two diseases in the population of forest areas of eight provinces in China by measuring seroprevalence of antibodies against B. burgdorferi and A. phagocytophilum. Methods Forest areas in 8 provinces were chosen for investigation using whole sampling and questionnaire survey methods. 3 669 serum samples from people in the forest areas were tested for the presence of antibodies by indirect immunofluorescent assay （IFA）. Results Seroprevalence against B. burgdorferi was 3% to 15% and against A. phagocytophilum was 2% to 18% in the study sites in the 8 provinces in China. We also found co-infection of B. burgdorferi and A. phagocytophilum in 7 of the 8 provinces （the exception being the Miyun area in Beijing）. The seroprevalence for both B. burgdorferi and A. phagocytophilum was significantly higher among people exposed to ticks than among people who were not exposed to ticks. Conclusion We conclude that both pathogens are endemic in the forest areas in the eight provinces, but the prevalence of B. burgdorferi and A. phagocytophilum differs between the provinces.

关键词： Borrelia burgdorferi Anaplasma phagocytophilum Co-infection Residents of forest areas

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