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Carbon fluxes and soil carbon dynamics along a gradient of biogeomorphic succession in alpine wetlands of Tibetan Plateau

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Fundamental Research 2023年第2期3卷 151-159页

作者： Hao Wang Lingfei Yu Litong Chen Zhenhua Zhang Xuefei Li Naishen Liang Changhui Peng Jin-Sheng He State Key Laboratory of Herbage Improvement and Grassland Agro-eco.ystems College of EcologyLanzhou UniversityLanzhou 730000China Institute of Eco.ogy College of Urban and Environmental Sciencesand Key Laboratory for Earth Surface Processes of the Ministry of EducationPeking UniversityBeijing 100871China State Key Laboratory of Vegetation and Environmental Change Institute of BotanyChinese Academy of SciencesBeijing 100093China Qinghai Haibei National Field Research Station of Alpine Grassland Eco.ystem and Key Laboratory of Adaptation and Evolution of Plateau BiotaNorthwest Institute of Plateau BiologyChinese Academy of SciencesXining 810008China Institute for Atmospheric and Earth System Research(INAR)/Physics Faculty of ScienceUniversity of HelsinkiP.O.Box 68Helsinki FI-00014Finland Earth System Division National Institute for Environmental StudiesTsukubaIbaraki 305-8506Japan g Department of Biology Sciences Institute of Environment SciencesUniversity of Quebec at MontrealMontrealQC H3C 3P8Canada State Key Laboratory of Herbage Improvement and Grassland Agro-eco.ystems College of Pastoral Agriculture Science and TechnologyLanzhou UniversityLanzhou 730000China

Hydrological changes under climate warming drive the biogeomorphic succession of wetlands and may trigger substantial carbon loss from the carbon-rich *** many studies have explored the responses of wetland carbon emissions to short-term hydrological change,it remains poorly understood how the carbon cycle evolves with hydrology-driven wetland ***,we used a space-for-time approach across hydrological gradients on the Tibetan Plateau to examine the dynamics of eco.ystem carbon fluxes(carbon dioxide(co.(2))and methane(CH4))and soil organic carbon pools during alpine wetland *** found that the succession from mesic meadow to fen changed the seasonality of both co.(2) and CH4 fluxes,which was related to the shift in plant co.munity co.position,enhanced regulation of soil hydrology and increasing co.tribution of spring-thaw *** paludification caused a switch from net uptake of gaseous carbon to net release on an annual timescale but produced a large accumulation of soil organic *** attempted to attribute the paradox between evidence from the carbon fluxes and pools to the lateral carbon input and the systematic changes of historical climate,given that the wetlands are spatially low-lying with strong temporal climate-carbon cycle *** findings demonstrate a systematic change in the carbon cycle with succession and suggest that biogeomorphic succession and lateral carbon flows are both important for understanding the long-term dynamics of wetland carbon footprints.

关键词： Carbon dioxide Methane Soil organic carbon Climate change Hydrological change

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Potential treatment approaches for malignant peritoneal mesothelioma: in vivo and in vitro experimental study of natural killer cell immunotherapy

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Cancer Biology & Medicine 2024年第11期 1078-1094页

作者： Heliang Wu Yi Wang Yulin Lin Ru Ma Xuemei Du Yandong Su Rui Yang Zhiran Yang Xinli Liang Yinguang Zhang Xiaoqing Liang Zhonghe Ji Chunning Lai Yajing Huang Yan Li Department of Peritoneal Cancer Surgery Beijing Shijitan Hospital Peking University Ninth School of Clinical Medicine Department of Peritoneal Cancer Surgery Beijing Shijitan Hospital Capital Medical University Department of Surgical Onco.ogy Beijing Tsinghua Changgung Hospital Tsinghua University Department of Hematology Fifth Medical Center of Chinese PLA General Hospital Department of Thoracic Surgery Zhongshan Hospital Fudan University (Xiamen Branch) Department of Pathology Beijing Friendship Hospital Capital Medical University Department of Thoracic Surgery Beijing Tiantan Hospital Capital Medical University Department of Onco.ogy Beijing Shijitan Hospital Capital Medical University Innovvy Biomedical Technology co. Ltd.

Objective: Malignant peritoneal mesothelioma(MPM) is a rare primary malignant tumor with an extremely poor prognosis that currently lacks effective treatment options. This study investigated the in vitro and in vivo efficacy of natural killer(NK) cells for treatment of ***: An in vitro study was co.ducted to assess the cytotoxicity of NK cells from umbilical co.d blood to MPM cells with the use of a high-co.tent imaging analysis system, the Cell co.nting Kit-8 assay, and Wright-Giemsa staining. The level of NK cell effector molecule expression was detected by flow cytometry and enzyme-linked immunosorbent assays. The ability of NK cells to kill MPM cells was determined based on live cell imaging, transmission electron microsco.y, and scanning electron microsco.y. An in vivo study was co.ducted to assess the efficacy and safety of NK cell therapy based on the experimental peritoneal cancer index, small animal magnetic resonance imaging, and co.ventional histopathologic, cytologic, and hematologic ***: NK cells effectively killed MPM cells through the release of effector molecules(granzyme B, perforin, interferon-γ, and tumor necrosis factor-α) in a dose-and density-dependent manner. The NK cell killing process potentially involved four dynamic steps: chemotaxis; hitting; adhesion; and penetration. NK cells significantly reduced the tumor burden, diminished ascites production, and extended survival with no significant hematologic toxicity or organ damage in NOG ***: NK cell immunotherapy inhibited proliferation of MPM cells in vitro and in vivo with a good safety profile.

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Cannabinoids function in defense against chewing herbivores in Cannabis sativa L.

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Horticulture Research 2023年第11期10卷 131-140页

作者： George M.Stack Stephen I.Snyder Jaco. A.Toth Michael A.Quade Jamie L.Crawford John K.McKay John Nicholas Jackowetz Ping Wang Glenn Philippe Julie L.Hansen Virginia M.Moore Jocelyn K.C.Rose Lawrence B.Smart Horticulture Section School of Integrative Plant ScienceCornell UniversityCornell AgriTechGenevaNY 14456United States Plant Biology Section School of Integrative Plant ScienceCornell UniversityIthacaNY 14853United States Plant Breeding Section School of Integrative Plant ScienceCornell UniversityIthacaNY 14853United States Department of Agricultural Biology Colorado State UniversityFort CollinsCO 80523United States Cirona Labs GenevaNY 14456United States Department of Entomology Cornell UniversityCornell AgriTechGenevaNY 14456United States

In the decades since the first cannabinoids were identified by scientists,research has focused almost exclusively on the function and capacity of cannabinoids asmedicines and intoxicants for humans and other *** little is knownabout the adaptive value of cannabinoid production,though several hypotheses have been proposed including protection from ultraviolet radiation,pathogens,and *** test the prediction that genotypeswith greater co.centrations of cannabinoidswill have reduced herbivory,a segregating F2 population of Cannabis sativa was leveraged to co.duct lab-and field-based bioassays investigating the function of cannabinoids in mediating interactions with chewing *** the field,foliar cannabinoid co.centration was inversely co.related with chewing herbivore *** detached leaves,Trichoplusia ni larvae co.sumed less leaf area and grew less when feeding on leaves with greater co.centrations of *** electron and light microsco.y were used to characterize variation in glandular trichome ***-free genotypes had trichomes that appeared *** isolate cannabinoids from co.founding factors,artificial insect diet was amended with cannabinoids in a range of physiologically relevant *** grew less and had lower rates of survival as cannabinoid co.centration *** results support the hypothesis that cannabinoids function in defense against chewing herbivores.

关键词： function founding ultraviolet

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Dynamic RNA profiles in the small intestinal epithelia of cats after Toxoplasma gondii infection

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Infectious Diseases of Poverty 2023年第4期12卷 63-82页

作者： Bintao Zhai Shi-Chen Xie Jiyu Zhang Jun-Jun He Xing-Quan Zhu Key Laboratory of Veterinary Pharmaceutical Development Ministry of Agriculture and Rural AffairsLanzhou Institute of Husbandry and Pharmaceutical SciencesChinese Academy of Agricultural SciencesLanzhou730050GansuPeople’s Republic of China State Key Laboratory for Animal Disease co.trol and Prevention Key Laboratory of Veterinary Parasitology of Gansu ProvinceLanzhou Veterinary Research InstituteChinese Academy of Agricultural SciencesLanzhou730046GansuPeople’s Republic of China Laboratory of Parasitic Diseases College of Veterinary MedicineShanxi Agricultural UniversityTaigu030801ShanxiPeople’s Republic of China Research Center for Parasites&Vectors College of Veterinary MedicineHunan Agricultural UniversityChangsha410128HunanPeople’s Republic of China Key Laboratory of Veterinary Public Health of Yunnan Province College of Veterinary MedicineYunnan Agricultural UniversityKunming650201YunnanPeople’s Republic of China

Background Felids are the only definitive hosts ofToxoplasma gondii. However, the biological features of the feline small intestine followingT. gondii infection are poorly understood. We investigated the changes in the expression of RNAs (including mRNAs, long non-co.ing RNAs and circular RNAs) in the small intestinal epithelia of cats followingT. gondii infection to improve our understanding of the life cycle ofT. gondii and cat responses toT. gondii *** Fifteen cats were randomly assigned to five groups, and the infection groups were inoculated with 600 tissue cysts of theT. gondii Pru strain by gavage. The small intestinal epithelia of cats were co.lected at 6, 10, 14, and 30 days post infection (DPI). Using high-throughput RNA sequencing (RNA-seq), we investigated the changes in RNA expression. The expression levels of differentially expressed (DE) genes and non-co.ing RNAs (ncRNAs) identified by RNA-seq were validated by quantitative reverse transcription PCR (qRT-PCR). Differential expression was determined using the DESeq R *** In total, 207 annotated lncRNAs, 20,552 novel lncRNAs, 3342 novel circRNAs and 19,409 mRNAs were identified. Among these, 70 to 344 DE mRNAs, lncRNAs and circRNAs were detected, and the post-cleavage binding sites between 725 ncRNAs and 2082 miRNAs were predicted. Using the co.location method, we predicted that a total of 235 lncRNAs target 1044 protein-co.ing genes, while the results of co.expression analysis revealed that 174 lncRNAs target 2097 mRNAs. Pathway enrichment analyses of the genes targeted by ncRNAs suggested that most ncRNAs were significantly enriched in immune or diseases-related pathways. NcRNA regulatory networks revealed that a single ncRNA co.ld be directly or indirectly regulated by multiple genes or ncRNAs that co.ld influence the immune response of cats. co.expression analysis showed that 242 circRNAs, mainly involved in immune responses, were significantly associated withT. gondii infe

关键词： Toxoplasma gondii Definitive host Cat Small intestinal epithelia Long non-co.ing RNA Circular RNA mRNA

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Relationship between MODIS-NDVI data and wheat yield: A case study in Northern Buenos Aires province, Argentina

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Information Processing in agriculture 2015年第2期2卷 73-84页

作者： Mariano F.Lopresti Carlos M.Di Bella Américo.J.Degioanni Instituto Nacional de Tecnología Agropecuaria(INTA) EEA PergaminoRuta 32 km 4.5Pergamino 2700Buenos AiresArgentina INTA-Castelar CNIAInstituto de Clima y AguaLas Cabanas y Los Reseros S/NHurlingham 1686Buenos AiresArgentina UNRC Facultad de Agronomía y VeterinariaDepartamento de Ecología AgrariaRuta Nacional 36 km 601Río Cuarto 5800Co´rdobaArgentina co.ICET–co.sejo Nacional de Investigaciones Científicas y Técnicas Argentina

In co.ntries like Argentina,whose eco.omy depends heavily on crop production,the estimation of harvests is an elementary *** providing objectivity,the use of remote sensing allows estimating yield in *** the time of maximum leaf area in wheat co.responds with the critical period of the crop,a good relationship is expected between the Normalized Difference Vegetation Index(NDVI)and *** present study was carried out in the North of Buenos Aires province,*** on the type of soil,the study area can be divided into two homogeneous subzones:a subzone with lower clay co.tent in the southwestand a subzone with higher clay co.tent in the *** growing seasons(2003–2011)were *** the first five years,an empirical model was calibrated and validated with field-observed wheat yields and MOD13q1 product-NDVI data,whereas in the other four years,the calibrated model was applied by means of yield maps and by co.paring with official *** MOD13q1 image co.responding to Julian day 289 showed the best fit between NDVI and yield to estimate wheat yield *** yield maps,better weather co.ditions showedhigher yields and higher soil productivity presented a greater proportion of the area occupied by higher *** department level,an R2 value of 0.75 was found after relating the estimation of the calibrated empirical model with official *** method used allows predicting wheat yield 30 days before *** yield maps,the NDVI perceived the temporal and spatial variability in the study area.

关键词： Remote sensing Wheat NDVI Yield Empirical models MODIS

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爱尔兰非木质生物质原料生产生物质颗粒燃料的潜力

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农业工程技术(新能源产业) 2012年第3期 47-47页

作者： Anthony Nolan Kevin Mc Donnell Ger J. Devlin John P. Carroll John Finnan 吴卓晶王应宽 Biosystems Engineering UCD School of AgricultureFood Science and Veterinary MedicineUniversity College Dublin Teagasc Crops Research Centre

燃料价格日益上涨和气候变化的威胁必然需要清洁的、可再生的独立能源。本研究应用ArcGIS9.2软件建立一个GIS模型,分析了以爱尔兰境内非木质生物质(包括小麦、燕麦、大麦和油菜的秸秆,柳树和芒属植物)为原料生产生物质颗粒燃料的情况。... 详细信息

燃料价格日益上涨和气候变化的威胁必然需要清洁的、可再生的独立能源。本研究应用ArcGIS9.2软件建立一个GIS模型,分析了以爱尔兰境内非木质生物质(包括小麦、燕麦、大麦和油菜的秸秆,柳树和芒属植物)为原料生产生物质颗粒燃料的情况。供热与发电行业将使用清洁的碳中性非木质生物质原料替代当前使用的化石燃料。本研究旨在分析确定爱尔兰境内生物质原料的总面积(公顷)数,并计算出非木质生物质原料的潜在产量。结果表明:谷类作物秸秆是生产生物质颗粒燃料潜力最大的生物质资源。爱尔兰东南部、西南部和中东部生物质产量最大,在这些地区生产生物质颗粒燃料可能最为经济可行。在供热与发电行业用非木质生物质取代矿物燃料对减少co.排放潜力巨大,其实可行。

关键词：国生物质颗粒燃料生产非木质生物质地理信息系统分析可再生能源 co.减排

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鸡舍内湿帘降温系统启动初期防温度骤降调控策略研究

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中国农业文摘（农业工程） 2018年第4期30卷 F0003-F0003页

作者： Hui X Li B M Xin H W Zheng W C Shi Z X Yang X co.lege of Water Resources and Civil Engineering China Agricultural University Key Laboratory of Agricultural Engineering in Structure and Environment Ministry of Agriculture Beijing 100083 China Beijing Engineering Research Center on Animal Healthy Environment Beijing 100083 China co.lege of Automation Henan University of Animal Husbandary and Economy Zhengzhou 450011 China Department of Agricultural and Biosystems Engineering Iowa State University Ames IA 50011 USA

前期测试表明，在中国干旱地区，湿帘降温系统启动初期在鸡群活动范同内普遍存在温度骤降（△Tsd）问题。短时间内大幅度的温度波动会对蛋鸡产生急性冷应激，这已引起广泛关注。本研究旨在构建一套新型防温度骤降调控系统（NCS），以避... 详细信息

前期测试表明，在中国干旱地区，湿帘降温系统启动初期在鸡群活动范同内普遍存在温度骤降（△Tsd）问题。短时间内大幅度的温度波动会对蛋鸡产生急性冷应激，这已引起广泛关注。本研究旨在构建一套新型防温度骤降调控系统（NCS），以避免或减少△Tsd且便于操作。

关键词：湿帘降温系统温度骤降调控策略鸡舍干旱地区温度波动调控系统冷应激

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Development of an automatic monitoring system for rice light-trap pests based on machine vision

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Journal of Integrative agriculture 2020年第10期19卷 2500-2513页

作者： YAO Qing FENG Jin TANG Jian XU Wei-gen ZHU Xu-hua YANG Bao-jun LU Jun XIE Yi-ze YAO Bo WU Shu-zhen KUAI Nai-yang WANG Li-jun School of Information Science and Technology Zhejiang Sci-Tech UniversityHangzhou 310018P.R.China State Key Laboratory of Rice Biology China National Rice Research InstituteHangzhou 310006P.R.China Plant Protection Quarantine and Pesticide Management Station of ZhejiangHangzhou 310020P.R.China Zhejiang Top Cloud-agri Technology co Ltd.Hangzhou 310015P.R.China Agricultural Technology Extension Center of Shangyu Shaoxing 312300P.R.China Agricultural Technology Extension Center of Keerqin Keerqin 137713P.R.China

Monitring pest populations in paddy fields is important to effectively implement integrated pest *** traps are widely used to monitor field pests all over the *** co.ventional light traps still involve manual identification of target pests from lots of trapped insects,which is time-co.suming,labor-intensive and error-prone,especially in pest peak *** this paper,we developed an automatic monitoring system for rice light-trap pests based on machine *** system is co.posed of an itelligent light trap,a co.puter or mobile phone client platform and a cloud *** light trap firstly traps,kills and disperses insects,then co.lects images of trapped insects and sends each image to the cloud *** target pests in images are automatically identifed and co.nted by pest identification models loaded in the *** avoid light-trap insects piling up,a vibration plate and a moving rotation co.veyor belt are adopted to disperse these trapped *** was a close co.relation(r=0.92)between our automatic and manual identification methods based on the daily pest number of one-year images from one light *** experiments demonstrated the effectiveness and accuracy of our automatic light trap monitoring system.

关键词： automatic monitoring system light trap rice pest machine vision image processing co.volutional neural network

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Successful transplantation of cryopreserved spermatogonia in Sebastes schlegelii:A simple and suitable alternative approach for co.servation of viviparous fish

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Water Biology and Security 2023年第3期2卷 75-86页

作者： Li Zhou Xueying Wang Shuran Du Shihong Xu Yanfeng Wang Zongcheng Song Qinghua Liu Jun Li The Key Laboratory of Experimental Marine Biology Center for Ocean Mega-ScienceInstitute of OceanologyChinese Academy of SciencesQingdao266071China co.lege of Life Science Ningde Normal UniversityEngineering Research Center of Mindong Aquatic Product Deep-ProcessingFujian Province UniversityNingde352100China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao266071China Weihai Shenghang Aquatic Product Science and Technology co.Ltd. Weihai264200China

Black rockfish(Sebastes schlegelii)is one of the most important marine eco.omic viviparous ***,germplasm degradation and genetic diversity reduction have occurred due to overfishing and long-term artificial *** cell transplantation co.bined with cryopreservation may be an alternative way to protect genetic ***,in viviparous fish that undertake fertilization and embryo development in vivo,transplantation is more difficult than in oviparous fish,including selection of transplantation stage,isolation of germ stem cells,and preparation of sterile *** seriously restricts the development of viviparous ***,in this study,we aimed to explore a transplantation method suitable for these *** cells were isolated from cryopreserved whole testes of 300–400g male Sebastes schlegelii in May,labeled by PKH26,and intra-peritoneally transplanted into allogeneic larvae at 5–10 days ***,the development of donor-derived cells in recipients were co.tinuously detected by fluorescence labeling,histology,microsatellite markers,and fecundity *** results showed that donors were rich in spermatogonia(75%)and recipients maintained a high survival rate after transplantation,with a rate of>20%at sexual ***,donor-derived cells successfully migrated(100%),co.onized,and inco.porated into the developing recipient gonad(93.33%).Finally,transplanted recipients co.ld normally develop and differentiate into male and female individuals,with donor-derived gametes found in 65.38%of mature *** the present study,we first establish a simple and suitable transplantation method for Sebastes schlegelii using immature males and specific larvae,which will serve as a promising tool in the protection of germplasm resources for this transplantation-restricted marine viviparous species.

关键词： Allogeneic transplantation Spermatogonia Sebastes schlegelii Germ cells Germplasm

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混合生长式膜生物反应器中导流板的流体力学影响

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中国给水排水 2018年第21期34卷 80-85页

作者：王博文孙安宁张国闽薛鑫 Ahmed Bakr 杨飞海南大学热带农林学院海南海口570228 北控水务集团〈海南〉有限公司海南海口570228 Bio Eco.Environmental Technologies co.Ltd. 德国

在中试规模的混合生长式膜生物反应器(HG-MBR)中,采用导流板对膜区和填料区的隔离网进行了优化研究,通过清水试验分析了导流板面积和位置对反应器流体力学特性的影响。结果表明,使用导流板可以改善反应器的流体力学特性,减小隔离网对填... 详细信息

在中试规模的混合生长式膜生物反应器(HG-MBR)中,采用导流板对膜区和填料区的隔离网进行了优化研究,通过清水试验分析了导流板面积和位置对反应器流体力学特性的影响。结果表明,使用导流板可以改善反应器的流体力学特性,减小隔离网对填料流动的负面效应,降低驱动填料流化所需的曝气量,促进填料在反应器内的循环流动,从而达到节能降耗的目的。优化运行结果表明,膜区和填料区之间的隔离网在上部采用导流板式封闭设计(面积约为0. 71 m^2),当填料填充率为50%时,驱动填料流动所需的曝气量可减少15%以上,节能效果明显。

关键词：混合生长式膜生物反应器填料导流板隔离网流体力学特性

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