Successful transplantation of cryopreserved spermatogonia in Sebastes schlegelii:A simple and suitable alternative approach for conservation of viviparous fish

作     者：Li Zhou Xueying Wang Shuran Du Shihong Xu Yanfeng Wang Zongcheng Song Qinghua Liu Jun Li 

作者机构：The Key Laboratory of Experimental Marine BiologyCenter for Ocean Mega-ScienceInstitute of OceanologyChinese Academy of SciencesQingdao266071China College of Life ScienceNingde Normal UniversityEngineering Research Center of Mindong Aquatic Product Deep-ProcessingFujian Province UniversityNingde352100China Laboratory for Marine Biology and BiotechnologyQingdao National Laboratory for Marine Science and TechnologyQingdao266071China Weihai Shenghang Aquatic Product Science and Technology Co.Ltd.Weihai264200China 

出 版 物：《Water Biology and Security》 (水生生物与安全（英文）)

年 卷 期：2023年第2卷第3期

页      面：75-86页

学科分类：0906[农学-兽医学] 09[农学] 

基　　金：This work was supported by the Shandong Province Natural Science Foundation(ZR2020KC038) Research and Development Program of Shandong Province(2021LZGC029) National Key Research and Development Program(2018YFD0901205,2018YFD0901204) China Agriculture Research System(CARS-47) 

主　　题：Allogeneic transplantation Spermatogonia Sebastes schlegelii Germ cells Germplasm 

摘      要：Black rockfish(Sebastes schlegelii)is one of the most important marine economic viviparous ***,germplasm degradation and genetic diversity reduction have occurred due to overfishing and long-term artificial *** cell transplantation combined with cryopreservation may be an alternative way to protect genetic ***,in viviparous fish that undertake fertilization and embryo development in vivo,transplantation is more difficult than in oviparous fish,including selection of transplantation stage,isolation of germ stem cells,and preparation of sterile *** seriously restricts the development of viviparous ***,in this study,we aimed to explore a transplantation method suitable for these *** cells were isolated from cryopreserved whole testes of 300–400​g male Sebastes schlegelii in May,labeled by PKH26,and intra-peritoneally transplanted into allogeneic larvae at 5–10 days ***,the development of donor-derived cells in recipients were continuously detected by fluorescence labeling,histology,microsatellite markers,and fecundity *** results showed that donors were rich in spermatogonia(75%)and recipients maintained a high survival rate after transplantation,with a rate of20%at sexual ***,donor-derived cells successfully migrated(100%),colonized,and incorporated into the developing recipient gonad(93.33%).Finally,transplanted recipients could normally develop and differentiate into male and female individuals,with donor-derived gametes found in 65.38%of mature *** the present study,we first establish a simple and suitable transplantation method for Sebastes schlegelii using immature males and specific larvae,which will serve as a promising tool in the protection of germplasm resources for this transplantation-restricted marine viviparous species.