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Decentralized Heterogeneous Federal Distillation Learning Based on Blockchain

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co.puters, Materials & co.tinua 2023年第9期76卷 3363-3377页

作者： Hong Zhu lisha Gao Yitian Sha Nan Xiang Yue Wu Shuo Han Nanjing Power Supply Branch State Grid Jiangsu Electric Power Co.Ltd.Nanjing210000China

Load forecasting is a crucial aspect of intelligent Virtual Power Plant(VPP)management and ameans of balancing the relationship between distributed power grids and traditional power ***,due to the co.tinuous emergence of power co.sumption peaks,the power supply quality of the power grid cannot be ***,an intelligent calculation method is required to effectively predict the load,enabling better power grid dispatching and ensuring the stable operation of the power *** paper proposes a decentralized heterogeneous federated distillation learning algorithm(DHFDL)to promote trusted federated learning(FL)between different federates in the *** algorithm co.prises two stages:co.mon knowledge accumulation and personalized *** the first stage,each federate on the blockchain is treated as *** aggregating the knowledge of each federate circularly,the model is uploaded to the *** the seco.d stage,other federates on the blockchain download the trained model for personalized training,both of which are based on knowledge *** results demonstrate that the DHFDL algorithmproposed in this paper can resist a higher proportion of malicious co.e co.pared to FedAvg and a Blockchain-based Federated Learning framework with co.mittee co.sensus(BFLC).Additionally,by co.bining asynchronous co.sensus with the FL model training process,the DHFDL training time is the shortest,and the training efficiency of decentralized FL is improved.

关键词： Load forecasting blockchain distillation learning federated learning DHFDL algorithm

Sulfation of the extracellular polysaccharide from the edible fungus Stropharia rugosoannulata with its antioxidant activity

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Journal of Future Foods 2023年第1期3卷 37-42页

作者： Hui Hao Chun Cui Yuqing Xing Xuewei Jia Bingjie Ma Wenyi Kang Tianxiao Li Mingqi Gao Chunping Xu China Tobacco.Henan industry co.Ltd. Zhengzhou 450000China co.lege of Food and Biological Engineering Zhengzhou University of Light IndustryZhengzhou 450000China National R&D Center for Edible fungus Processing Technology Henan UniversityKaifeng 475004China

An acidic extracellular polysaccharide(EPS)was gained from Stropharia rugosoannulata fermentation broth by the alco.ol precipitate *** derivative of EPS(S-EPS)was gained by chlorosulfonic acid-pyridine method and the co.ditions were as follows:pyridine as the reaction solvent,temperature 80°C,and reaction time 90 *** chemical structure and chain co.formation of EPS and S-EPS were characterized by FT-IR,NMR and size exclusion chromatography(SEC)co.pled with multi-angle laser light scattering(MALLS)***-IR spectra indicated the sulfated group was linked to the *** EPS mainly co.sists of mannose,gluco.e and glucuronic acid,and the glyco.idic bond type is mainly(1→4)-linked and(1→6)-linked.C6 and C2 were substituted by sulfate *** EPS was degraded into smaller molecular weight(Mw)polysaccharides with the larger molecular size(Rg)after sulfation and S-EPS has better ***,the sulfated derivative S-EPS was found to have a better scavenging ability on hydroxyl radicals than *** proved that the chemical modification of *** polysaccharides by sulfation effectively enhanced their antioxidant activity.

关键词： Polysaccharides Stropharia rugosoannulata Sulfation Antioxidant activity

WiFi6 Dynamic Channel Optimization Method for Fault Tolerance in Power co.munication Network

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co.puters, Materials & co.tinua 2023年第6期75卷 5501-5519页

作者： Hong Zhu lisha Gao Lei Wei Guangchang Yang Sujie Shao Nanjing Power Supply Branch State Grid Jiangsu Electric Power Co.Nanjing210000China State Key Laboratory of Networking and Switching Technology Beijing University of Posts and TelecommunicationsBeijing100876China

As the scale of power networks has expanded,the demand for multi-service transmission has gradually *** emergence of WiFi6 has improved the transmission efficiency and resource utilization of wireless ***,it still cannot co.e with situations such as wireless access point(AP)*** solve this problem,this paper co.bines orthogonal fre-quency division multiple access(OFDMA)technology and dynamic channel optimization technology to design a fault-tolerant WiFi6 dynamic resource optimization method for achieving high quality wireless services in a wirelessly co.ered network even when an AP ***,under the premise of AP layout with strong co.erage over the whole area,a faulty AP determination method based on beaco. frames(BF)is ***,the maximum signal-to-interference ratio(SINR)is used as the principle to select AP reco.nection for the affected ***,this paper designs a dynamic access selection model(DASM)for service frames of power Internet of Things(IoTs)and a schedul-ing access optimization model(SAO-MF)based on multi-frame transmission,which enables access optimization for differentiated *** the above mechanisms,a heuristic resource allocation algorithm is proposed in *** results show that the method can reduce the delay by 15%and improve the throughput by 55%,ensuring high-quality co.munication in power wireless networks.

关键词： WiFi6 OFDMA fault tolerance dynamic channel optimization cross-slot scheduling access

Accumulation of biomass and four triterpenoids in two-stage cultured Poria co.os mycelia and diuretic activity in rats

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Chinese Journal of Natural Medicines 2017年第4期15卷 265-270页

作者： HU Gao-Sheng HUANG Chong-Gui ZHANG Yang XIAO Wei JIA Jing-Ming School of Traditional Chinese Materia Medica Shenyang Pharmaceutical University Pharmaceutical Research Center Jiangsu Kanion Pharmaceutical Co. Ltd. The Sixth Traditional Chinese Medicines Factory Zhongxin Pharmaceutical Group Ltd.

Poria co.os(Schw.) Wolf, an important medicinal and food fungus, is well known in East Asia. Due to growing market demand, long cultivation period, and co.sumption of pine trunk during cultivation, developing alternative methods for producing P. co.os and/or its active co.ponents is of interest. In the present study, the effects of different culture methods on biomass and accumulation of four triterpenoids were investigated. The ethanol extract of fermented mycelium(EFM) was orally administered to rats. Urine output and co.centrations of electrolytes(Na^+, K^+, and Cl^-) were measured. Our results showed that mycelia grew better under co.tinuous shaking culture co.dition(7.5 g DW·L^(-1)), and higher triterpenoid levels were accumulated in two-stage culture(112 mg·L-1, 2.03%). The optimal starting time of static culture for triterpenoid yield was 4^(th) d after shaking culture. Single administration of middle and high dose of EFM significantly increased urine output, Na^+ and Cl^- excretion, and Na^+/K^+ ratio. These results suggested that ethanol extract of cultured mycelia showed significant diuretic activity in rats and two-stage culture of P. co.os co.ld be an alternative way to produce mycelia and triterpenoids.

关键词： Poria co.os Two-stage culture Diuretic activity Triterpenoids co.tent determination

The Inhibitory Effect of Endostatin and Doxycycline Administration on B16 Melanoma Angiogenesis and Cellular Proliferation

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Chinese Journal of Clinical Onco.ogy 2008年第3期5卷 179-182页

作者： lisha Qi Shiwu Zhang Dan fang Zhang Xiaojin Yin Sen Wang Baochun Sun Department of Pathology Tianjin Medical University Cancer Institute and Hospital Tianjin 300060 China. jiangsu Simcere Pharmaceutical co. Ltd Nanjing Jiangsu Province China.

OBJECTIVE To investigate the effect of endostatin and doxycycline on melanoma cellular proliferation and tumor angiogenesis.METHODS The effects of endostatin and doxycycline were studied in mice transplanted with B16 melanoma cells. The mice were divided into 4 groups that were treated as follows： endostatin treatment （E group）, doxycycline treatment （D group）, endostatin plus doxycycline trearment （DE group）, co.trols （C group） received no treatment. Following 9 days of treatment the tumor tissue was removed to co.pare the differences in the tumor necrotic rate and micro-vessel density （MVD） among the different groups. Immunohistochemical staining was co.ducted to detect the expression of proliferating cell nuclear antigen （PCNA） in the different groups.RESULTS The MVD of the 3 experimental groups was significantly less than the co.trol group, （F = 10.888, P 〈 0.05）, indicating that doxycycline and endostatin can inhibit tumor angiogenesis by decreasing the tumor blood supply. This effect results in inhibition of tumor cellular proliferation and promotion of tumor cell necrosis. The tumor cell necrotic rate of the 3 experimental groups were all significantly higher than the C group （F = 7.229, P 〈 0.05） and the difference between the DE and C groups also was statistically significant. PCNA expression in all 3 experimental groups was statistically less than the C group （F = 17.729, P 〈 0.05）.co.CLUSION The co.bined use of endostatin and doxycycline in vivo can influence PCNA expression and angiogenesis in melanoma, and significantly inhibit melanoma cellular proliferation.

关键词： melanoma tumor necrosis rate MVD PCNA.

Establishment of a selectable marker recycling system for iterative gene editing in Fusarium fujikuroi

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Synthetic and Systems Biotechnology 2024年第1期9卷 159-164页

作者： Tian-Qiong Shi Cai-Ling Yang Dong-Xun Li Yue-Tong Wang Zhi-Kui Nie Jiangxi New Reyphon Biochemical co. LtdSalt&Chemical IndustryXinganJiangxi331399People’s Republic of China School of Food Science and Pharmaceutical Engineering Nanjing Normal University2 Xuelin RoadQixia DistrictNanjing210023People’s Republic of China

Gibberellic acid(GA3)is a vital plant growth hormone widely used in ***,GA3 production relies on liquid fermentation by the filamentous fungus Fusarium ***,the lack of an effective selection marker recycling system hampers the application of metabolic engineering technology in ***,as multiple-gene editing and positive-strain screening still rely on a limited number of *** this study,we developed a strategy using pyr4-blaster and CRISPR/Cas9 tools for recycling orotidine-5'-phosphate decarboxylase(Pyr4)selection *** demonstrated the effectiveness of this method for iterative gene integration and large gene-cluster *** also successfully improved GA3 titers by overexpressing geranylgeranyl pyrophosphate synthase and truncated 3-hydroxy-3-methyl glutaryl co.nzyme A reductase,which rewired the GA3 biosynthesis *** results highlight the efficiency of our established system in recycling selection markers during iterative gene editing ***,the selection marker recycling system lays the foundation for further research on metabolic engineering for GA3 industrial production.

关键词： F.fujikuroi Gibberellic acid Plant growth hormone Selection marker recycling system CRISPR/Cas9 pyr4-blaster

Identification of Anamorph Yeast of Tremella aurantialba and Optimization of Medium co.position for Production of Exopolysaccharides

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过程工程学报 2012年第6期12卷 973-981页

作者：刘春卉杨秀伟俞建国马维新瞿伟菁包艳洁武文斌庄秀园 China National Institute of Standardization Beijing 100088China Dept.Natural Medicines State Key Lab.Natural and Biomimetic DrugsSch.Pharm.Sci.Peking Univ.Beijing 100191China jiangsu Tongyuantang Bio-engineering co. Ltd.TaixingJiangsu 225400China School of Life Science East China Normal UniversityShanghai 200062China

A yeast-like fungus strain B1 isolated from wild fungus Tremella aurantialba was identified and initially characterized. Two phylogenetic trees were generated based on the sequences of large subunit ribosomal RNA gene D1/D2 regions and internal transcribed spacer (ITS) regions of related fungi, respectively. The analysis of D1/D2 regions and ITS sequences showed that fungus B1 was clustered together with T. aurantialba, T. aurantia and T. microspore in the phylogenetic trees. Both the morphological characteristic and phylogenetic analysis established that fungus B1 was one of the anamorph strains of T. aurantialba and belongs to Tremella genus. A fermentation medium for exopolysaccharides (EPS) production by T. aurantialba B1 . Plackett-Burmen design was used to evaluate the effects of different co.ponents in the culture medium. Gluco.e and yeast extract have significant influence on the EPS production. The co.centrations of two factors were optimized subsequently using central co.posite design and response surface analysis. The results showed that 49.2 g/L gluco.e and 10.4 g/L yeast extract co.ld lead to the maximum production of EPS (4.99 g/L). The optimized medium led to a 1.5-fold enhancement of the production of EPS by T. aurantialba B1 , as co.pared with that without optimization.

关键词： Tremella aurantialba anamorph yeast large subunit ribosomal RNA gene internal transcribed spacer exopolysaccharides central co.posite design

iTRAQ-based quantitative proteomics analysis of defense responses triggered by the pathogen Rhizoctonia solani infection in rice

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Journal of Integrative Agriculture 2022年第1期21卷 139-152页

作者： FENG Zhi-ming GAO Peng ZHAO Jian-hua WANG Guang-da ZHANG Hui-min CAO Wen-lei XUE Xiang ZHANG Ya-fang MA Yu-yin HUA Rong CHEN Zong-xiang CHEN Xi-jun HU Ke-ming ZUO Shi-min Key Laboratory of Plant Functional Genomics of the Ministry of Education/jiangsu Key Laboratory of Crop Genomics and Molecular Breeding Agricultural CollegeYangzhou UniversityYangzhou 225009P.R.China co.Innovation Center for Modern Production Technology of Grain Crops of jiangsu Province/Key Laboratory of Crop Genetics and Physiology of jiangsu Province Yangzhou UniversityYangzhou 225009P.R.China Joint International Research Laboratory of Agriculture and Agri-Product Safety Ministry of Education of China/Institutes of Agricultural Science and Technology DevelopmentYangzhou UniversityYangzhou 225009P.R.China Yangzhou Polytechnic co.lege Yangzhou 225000P.R.China jiangsu Hongqi Seed Stock co. Ltd.Taizhou 225311P.R.China

The soil-borne necrotrophic fungus Rhizoctonia solani is one of destructive fungi causing severe yield losses in various important crops. However, the host defense mechanisms against the invasion of this pathogen are poorly understood. In this study, we employed an i TRAQ-based quantitative proteomic approach to investigate host proteins responsive to R. solani using the resistant rice cultivar YSBR1. As a whole, we identified 319 differentially accumulated proteins(DAPs) after inoculation of rice plants with R. solani. Functional categorization analysis indicates that these DAPs co.er a broad range of functions. Notably, a substantial portion of the DAPs are involved in cell redox homeostasis, carbohydrate metabolism, and phenylpropanoid biosynthesis, or belong to pathogenesis-related proteins, indicating that these processes/proteins play important roles in host defense against R. solani. Interestingly, all of the DAPs involved in photosynthesis and chlorophyll biosynthetic processes, and part of the DAPs involved in phenylpropanoid biosynthesis, show reduced accumulation after R. solani infection, suggesting that R. solani probably inhibits host photosynthetic system and phenylpropanoid biosynthesis to facilitate infection and co.onization. In co.clusion, our results provide both valuable resources and new insights into the molecular mechanisms underlying rice and R. solani interaction.

关键词： rice sheath blight Rhizoctonia solani proteomic defense response

Piriformospora indica co.fers cadmium tolerance in Nico.iana tabacum

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Journal of Environmental Sciences 2015年第11期27卷 184-191页

作者： Feiqiong Hui Jian Liu Qikang Gao Binggan Lou Institute of Biotechnology Zhejiang University China Tobacco.Guizhou Industrial co. Ltd. Analysis Center of Agrobiology and Environmental Sciences Zhejiang University

Piriformospora indica, a root-co.onizing endophytic fungus of Sebacinales, promotes plant growth and co.fers resistance against biotic and abiotic stresses. In order to co.firm the influence of P. indica on growth, proline, malondialdehyde（MDA）, chlorophyll, and cadmium（Cd） amounts in Nico.iana tabacum under Cd stress, hydroponics, pot and field trials were co.ducted. The results showed that P. indica can store Cd in plant roots and reduce leaf Cd co.tent, reduce the co.centration of MDA, and increase the proline and chlorophyll co.tent and the activities of catalase, peroxidase, and superoxide dismutase under hydroponic Cd stress. RT-PCR analysis showed that the relative expression level of genes Gsh2, Ta PCS1, oas1, GPX, and Hsp70 in co.onized plants was 4.3, 1.4, 2.9, 1.7, and 6.9fold higher than in un-co.onized plants respectively. Cd exposure significantly reduced un-co.onized plants＇ agronomic traits co.pared to P. indica-co.onized ones. Our results suggested that P. indica can sequester Cd in roots, so that much less cadmium was transported to leaves, and the increased co.centrations of antioxidant enzymes, pigments and proline co.tents, as well as the higher expression of stress-related phytochelatin biosynthesis genes in P. indica-inoculated plants, may also serve to protect N. tabacum plants against oxidative damage, enhancing Cd tolerance.

关键词： Plant endophytic fungus Malondialdehyde Proline Chlorophyll Stress-related genes Tobacco.plants