Accumulation of biomass and four triterpenoids in two-stage cultured Poria cocos mycelia and diuretic activity in rats

作     者：HU Gao-Sheng HUANG Chong-Gui ZHANG Yang XIAO Wei JIA Jing-Ming 

作者机构：School of Traditional Chinese Materia Medica Shenyang Pharmaceutical University Pharmaceutical Research Center Jiangsu Kanion Pharmaceutical Co. Ltd. The Sixth Traditional Chinese Medicines FactoryZhongxin Pharmaceutical Group Ltd. 

出 版 物：《Chinese Journal of Natural Medicines》 (中国天然药物（英文版）)

年 卷 期：2017年第15卷第4期

页      面：265-270页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 1005[医学-中医学] 100706[医学-药理学] 1002[医学-临床医学] 0703[理学-化学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：financially supported by the post-doctoral program of 54~(th) China Postdoctoral Science Foundation(No.2013M541255) Career Development Support Program for Young and Middle-aged Teachers of Shenyang Pharmaceutical University(No.ZQN2015034) the funds from Shenyang Pharmaceutical University Kanion Pharmaceutical Co.Ltd 

主　　题：Poria cocos Two-stage culture Diuretic activity Triterpenoids Content determination 

摘      要：Poria cocos(Schw.) Wolf, an important medicinal and food fungus, is well known in East Asia. Due to growing market demand, long cultivation period, and consumption of pine trunk during cultivation, developing alternative methods for producing P. cocos and/or its active components is of interest. In the present study, the effects of different culture methods on biomass and accumulation of four triterpenoids were investigated. The ethanol extract of fermented mycelium(EFM) was orally administered to rats. Urine output and concentrations of electrolytes(Na^+, K^+, and Cl^-) were measured. Our results showed that mycelia grew better under continuous shaking culture condition(7.5 g DW·L^(-1)), and higher triterpenoid levels were accumulated in two-stage culture(112 mg·L-1, 2.03%). The optimal starting time of static culture for triterpenoid yield was 4^(th) d after shaking culture. Single administration of middle and high dose of EFM significantly increased urine output, Na^+ and Cl^- excretion, and Na^+/K^+ ratio. These results suggested that ethanol extract of cultured mycelia showed significant diuretic activity in rats and two-stage culture of P. cocos could be an alternative way to produce mycelia and triterpenoids.