检索结果-南通市图书馆

Establishment of a humanized mouse model using steady-state peripheral blood-derived hematopoietic stem and progenitor cells facilitates screening of cancer-targeted T-cell repertoires

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

cancer Innovation 2024年第3期3卷 1-21页

作者： Yulin Xu Wei Shan Qian Luo Meng Zhang Dawei Huo Yijin chen Honghu Li Yishan Ye Xiaohong Yu Yi Luo He Huang Bone Marrow transplantation center The First Affiliated HospitalZhejiang University School of MedicineHangzhouChina Liangzhu Laboratory Zhejiang UniversityHangzhouChina Institute of Hematology Zhejiang UniversityHangzhouChina Zhejiang Province Engineering Research center for Stem cell and Immunity Therapy HangzhouChina School of Medicine Zhejiang UniversityHangzhouChina

Background:cancer-targeted T-cell receptor T(TcR-T)cells hold promise in treating cancers such as hematological malignancies and breast ***,approaches to obtain cancer-reactive TcR-T cells have been ***:Here,we developed a novel strategy to screen for cancer-targeted TcR-T cells using a special humanized mouse model with person-specific immune *** steady-state circulating hematopoietic stem and progenitor cells were expanded via three-dimensional culture of steady-state peripheral blood mononuclear cells,and then the expanded cells were applied to establish humanized *** human immune system was evaluated according to the kinetics of dendritic cells,monocytes,T-cell subsets,and *** fully stimulate the immune response and to obtain B-cell precursor NAML-6-and triple-negative breast cancer MDA-MB-231-targeted TcR-T cells,we used the inactivated cells above to treat humanized mice twice a day every 7 ***,human T cells were processed for TcRβ-chain(TRB)sequencing *** the repertoires had been constructed,features such as the fraction,diversity,and immune signature were ***:The results demonstrated an increase in diversity and clonality of T cells after *** preferential usage and features of TRBV,TRBj,and the V–j.combination were also *** stress also induced highly clonal Science and Technology,Grant/Award Number:2021c03010;Zhejiang Provincial Natural Science Foundation of china,Grant/Award Numbers:LTGY24H080003,LY21H080004 *** burden and survival analysis demonstrated that stress induction could significantly inhibit the growth of subsequently transfused live tumor cells and prolong the survival of the humanized ***:We constructed a personalized humanized mouse model to screen cancer-targeted TcR-T *** platform provides an effective source of cancer-targeted TcR-T cells and allows for the design of patient-specific engineered T cells.

关键词： cancer-targeted T-cell receptor T(TcR-T)cells circulating hematopoietic stem and progenitor cells(HSPcs) humanized mouse model steady-state peripheral blood T-cell receptorβ-chain(TRB) three-dimensional culture

The HIF-1α/PLOD2 axis integrates extracellular matrix organization and cell metabolism leading to aberrant musculoskeletal repair

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bone Research 2024年第2期12卷 289-301页

作者： Heeseog Kang Amy L.Strong Yuxiao Sun Lei Guo conan juan Alec c.Bancroft ji Hae choi chase A.Pagani Aysel A.Fernandes Michael Woodard juhoon Lee Sowmya Ramesh Aaron W.james David Hudson Kevin N.Dalby Lin Xu Robert j.Tower Benjamin Levi center for Organogenesis Regeneration and TraumaDepartment of SurgeryUniversity of Texas SouthwesternDallasTX 75390USA Section of Plastic Surgery Department of SurgeryUniversity of MichiganAnn ArborMI 48109USA Quantitative Biomedical Research center Peter O’Donnell Jr.School of Public HealthUniversity of Texas SouthwesternDallasTX 75390USA Department of Orthopedics and Sports Medicine University of WashingtonSeattleWA 98195USA Division of chemical Biology and Medicinal chemistry College of PharmacyUniversity of Texas at AustinAustinTX 78712USA Department of Pathology Johns Hopkins UniversityBaltimoreMD 21218USA

While hypoxic signaling has been shown to play a role in many cellular processes,its role in metabolism-linked extracellular matrix(EcM)organization and downstream processes of cell fate after musculoskeletal injury remains to be ***(HO)is a debilitating condition where abnormal bone formation occurs within extra-skeletal *** andhypoxia-inducible factor 1α(HIF-1α)activation have been shown to promote ***,the underlying molecular mechanisms bywhich the HIF-1αpathway in mesenchymal progenitor cells(MPcs)contributes to pathologic bone formation remain to ***,we used a proven mouse injury-induced HO model to investigate the role of HIF-1αon aberrant cell ***-cell RNA sequencing(scRNA-seq)and spatial transcriptomics analyses of the HO site,we found that collagen EcM organizationis the most highly up-regulated biological process in *** mesenchymal cell-specific deletion of Hif1α(Hoxa11-creER^(T2);Hif1a^(fl/fl))significantly mitigated HO in ***-seq analysis of these Hoxa11-creER^(T2);Hif1a^(fl/fl)mice identified the PLOD2/LOXpathway for collagen cross-linking as downstream of the HIF-1αregulation of ***,our scRNA-seq data and mechanisticstudies further uncovered that glucose metabolism in MPcs is most highly impacted by HIF-1α*** a translational aspect,a pan-LOX inhibitor significantly decreased HO.A newly screened compound revealed that the inhibition of PLOD2 activity in MPcssignificantly decreased osteogenic differentiation and glycolytic *** suggests that the HIF-1α/PLOD2/LOX axis linked tometabolism regulates HO-forming MPc *** results suggest that the HIF-1α/PLOD2/LOX pathway represents a promisingstrategy to mitigate HO formation.

关键词： metabolism skeletal aberrant

Liver transplantation: current status and challenges

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

World journal of Gastroenterology 2016年第18期22卷 4438-4445页

作者： caroline c jadlowiec Timucin Taner William j. von Liebig transplantation center Division of Transplant Surgery Department of SurgeryMayo Clinic

Great progress has been made in the field of liver transplantation over the past two decades. This progress, however, also brings up the next set of challenges: First, organ shortage remains a major limitation, and accounts for a large proportion of wait list mortality. While living donation has successfully increased the total number of liver transplants done in Asian countries, the total number of such transplants has been stagnant in the western hemisphere. As such, there has been a significant effort over the past decade to increase the existing deceased donor pool. This effort has resulted in a greater use of liver allografts following donation after cardiac death(DcD) along with marginal and extended criteria donors. Improved understanding of the pathophysiology of liver allografts procured after circulatory arrest has not only resulted in better selection and management of DcD donors, but has also helped in the development of mechanical perfusion strategies. Early outcomes demonstrating the clinical applicability of both hypothermic and normothermic perfusion and its potential to impact patient survival and allograft function have generated much interest. Second, long-term outcomes of liver transplant recipients have not improved significantly, as recipients continue to succumb to complications of long-term immunosuppression, such as infection, malignancy and renal failure. Furthermore, recent evidence suggests that chronic immune-mediated injury to the liver may also impact graft function.

关键词： Donation after cardiac death Mechanical perfusion Renal-sparing immunosuppression Antibody-mediated rej.ction

Spatial TIME landscape and its prognostic value in the lung and brain tumor:location matters

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2023年第6期8卷 2437-2439页

作者： Yunke Wang Anne E.Geller jun Yan Division of Immunotherapy The Hiram C.PolkJr.MD Department of SurgeryImmuno-Oncology ProgramBrown Cancer CenterUniversity of Louisville School of MedicineLouisvilleKYUSA Department of Microbiology and Immunology University of Louisville School of MedicineLouisvilleKYUSA

In two recently published papers in Nature,^(1,2)Logan Walsh and colleagues utilized imaging mass cytometry(IMc)to create comprehensive single-cell spatial landscapes of the tumor immune microenvironment(TIME)in lung tumors from 416 lung adenocarcinoma(LUAD)patients,as well as brain tumors from 139 high-grade glioma and 46 brain metastasis(BrM)*** conducting a thorough analysis to characterize cellular spatial interactions and neighborhood patterns,and correlating these spatial features with clinical outcomes(Fig.1),the researchers gained novel insights into the complexities of tumoral ***,they identified two sets of clinically relevant predictive immune markers that could be used to assist in guiding clinical decisions.

关键词： lung landscape gained

ca^(2+) binding to the c_(2)E domain of otoferlin is required for hair cell exocytosis and hearing

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Protein & cell 2024年第4期15卷 305-312页

作者： Han chen Mehar Monga Qinghua Fang Loujin Slitin jakob Neef Shashank S.chepurwar Regina célia Mingroni Netto Karina Lezirovitz Alfredo Tabith,jr. Fritz Benseler Nils Brose Kathrin Kusch carolin Wichmann Nicola Strenzke Barbara Vona julia Preobraschenski Tobias Moser Institute for Auditory Neuroscience and InnerEarLab University Medical Center Göttingen37075 GöttingenGermany collaborative Research center 889 University of Göttingen37075 GöttingenGermany Auditory Neuroscience and Synaptic Nanophysiology Group Max Planck Institute for Multidisciplinary Sciences37075 GöttingenGermany Göttingen Graduate center for Neurosciences Biophysics and Molecular BiosciencesUniversity of Göttingen37075 GöttingenGermany Biochemistry of Membrane Dynamics Group Institute for Auditory NeuroscienceUniversity Medical Center Göttingen37075 GöttingenGermany Molecular Architecture of Synapses Group Institute for Auditory Neuroscience and InnerEarLabUniversity Medical Center Göttingen37075 GöttingenGermany center for Biostructural Imaging of Neurodegeneration University Medical Center Göttingen37075 GöttingenGermany Auditory Systems Physiology Group Institute for Auditory Neuroscience and InnerEarLabUniversity Medical Center Göttingen37075 GöttingenGermany Departamento de Genética e Biologia Evolutiva Centro de Pesquisas sobre o Genoma Humano e Células-TroncoInstituto de BiociênciasUniversidade de São PauloSão Paulo 05508-220Brazil Laboratório de Otorrinolaringologia/LIM32 Faculdade de MedicinaHospital das ClínicasUniversidade de São PauloSão PauloSP 05508-220Brazil DERDIc Pontifícia Universidade Católica de São PauloSão Paulo 05508-220Brazil Department of Molecular Neurobiology Max Planck Institute for Multidisciplinary Sciences37075 GöttingenGermany Multiscale Bioimaging cluster of Excellence(MBExc) University of Göttingen37075 GöttingenGermany Functional Auditory Genomics Group Auditory Neuroscience and Optogenetics LaboratoryGerman Primate Center37077 GöttingenGermany Hearing Genomics Group Institute for Auditory Neuroscience and InnerEarLabUniversity Medical Center Göttingen37075 GöttingenGermany Institute of Human Genetics University Medical Center Göttingen37075 GöttingenGermany

Dear Editor,Afferent synapses of cochlear inner hair cells(IHcs)employ a unique molecular machinery(see extended background in Supplementary Materials).Otoferlin is a key player in this machinery and its defects cause human auditory synaptopathy(Moser and Starr,2016).Otoferlin,a tail-anchored(Vogl et al.,2016)multi-c_(2)-domain protein(Fig.1Ai)specific to hair cells(Roux et al.,2006),is a member of the ferlin protein family involved in membrane trafficking and repair that are of major disease relevance(Pangršičet al.,2012),also see Supplementary *** is distributed broadly within IHcs(Fig.2Ai-Aiii;Pangrsic et al.,2010;Roux et al.,2006).

关键词： auditory hair

Unexpected Breg-NK crosstalk in STING agonist therapy

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

cellular & Molecular Immunology 2022年第12期19卷 1330-1332页

In a recent paper published in Nature,Li et *** that resistance to stimulator of interferon genes(STING)monotherapy in solid tumors is attributable to the unexpected surge of IL-35-producing regulatory B(Breg)cells that impair NK-cell cytotoxicity[1].They depict STING-induced Breg-NK-cell crosstalk and propose that combining the STING agonist 2'3'cyclic GMP-AMP(cGAMP)with anti-IL-35 blockade is a promising strategy to overcome tumor immunosuppression,especially in pancreatic cancers[1].

关键词： Breg interferon

Revisiting the liver’s role in transplant alloimmunity

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World journal of Gastroenterology 2019年第25期25卷 3123-3135页

作者： Nitin Abrol caroline c jadlowiec Timucin Taner William j.von Liebig center for transplantation and clinical Regeneration Massyo ClinicRochesterMN 55905United States Transplant center Mayo ClinicPhoenixAZ 85259United States

The transplanted liver can modulate the recipient immune system to induce tolerance after *** phenomenon was observed nearly five decades ***,the liver’s role in multivisceral transplantation was recognized,as it has a protective role in preventing rej.ction of simultaneously transplanted solid organs such as kidney and *** liver has a unique architecture and is home to many cells involved in immunity and *** transplantation,these cells migrate from the liver into the *** studies identified chimerism as an important mechanism by which the liver modulates the human immune *** studies on human T-cell subtypes,cytokine expression,and gene expression in the allograft have expanded our knowledge on the potential mechanisms underlying *** this article,we discuss the privileged state of liver transplantation compared to other solid organ transplantation,the liver allograft’s role in multivisceral transplantation,various cells in the liver involved in immune responses,and the potential mechanisms underlying immunomodulation of host alloresponses.

关键词： Liver transplantation Alloimmunity Liver-kidney transplant Tolerance Rej.ction

PTPROt maintains T cell immunity in the microenvironment of hepatocellular carcinoma

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

分子细胞生物学报:英文版 2015年第4期7卷 338-350页

作者： jiajie Hou Lei Deng Han Zhuo Zhe Lin Yun chen Runqiu jiang Dianyu chen Xudong Zhang Xingxu Huang Beicheng Sun Liver transplantation center of the First Affiliated Hospital Nanjing Medical UniversityNanjingChina MOE Key Laboratory of Model Animal for Disease Study Model Animal Research Center of Nanjing UniversityNanjingChina Liver transplantation center Renji HospitalSchool of MedicineShanghai Jiaotong UniversityShanghaiChina

Intratumoral T cells play a central role in anti-tumor immunity,and the balance between T effector cells(Teff)and regulatory T cells(Treg)affects the prognosis of cancer ***,educated by tumor microenvironment,T cells frequently fail in their *** this study,we aimed to investigate the role of truncated isoform of protein tyrosine phosphatase receptor-typeO(PTPROt)in T cell-mediated anti-tumor *** recruited 70 hepatocellular carcinoma(Hcc)patients and 30 healthy volunteers for clinical investigation,and analyzed cellular tumor immunity by using ptpro^(-/-) c57BL/6 mice and NOD/ScID *** expression was significantly downregulated in human Hcc-infiltrating T cells due to the hypoxia microenvironment;PTPROt expression highly correlated with the intratumoral Teff/Treg ratio and clinicopathologic ***,PTPROt deficiency attenuated T cell-mediated anti-tumor immunity and remarkably promoted mouse Hcc ***,deletion of PTPROt decreased Teff quantity and quality through phosphorylation of lymphocyte-specific tyrosine kinase,but increased Treg differentiation through phosphorylation of signal transducer and activator of transcription *** support of the Teff/Treg homeostasis,PTPROt serves as an important tumor suppressor in Hcc microenvironment.

关键词： PTPROt Teff Treg hepatocellular carcinoma

Viral hepatitis:A narrative review of hepatitis A–E

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World journal of Meta-Analysis 2022年第3期10卷 99-121页

作者： Zunirah Ahmed Akshay Shetty David W Victor Sudha Kodali Division of Gastroenterology and Hepatology Underwood Center for Digestive DisordersHouston Methodist HospitalHoustonTX 77030United States Department of Gastroenterology and Hepatology University of CaliforniaLos AngelesCA 90095United States Department of Hepatology J C Walter Jr Transplant CenterSherrie and Alan Conover Center for Liver Disease and TransplantationWeill Cornell Medical CollegeHoustonTX 77030United States

Viral hepatitis continues to be a major health concern leading to hepatic decompensation ranging from acute hepatitis to cirrhosis and hepatocellular *** hepatic and extrahepatic manifestations are not only debilitating but also associated with a significant economic *** the last two decades,the field of virology has made significant breakthroughs leading to a better understanding of the pathophysiology of viral hepatitis,which in turn has led to new therapeutic *** advent of direct-acting antiviral agents changed the landscape of hepatitis c virus(HcV)therapy,and new drugs are in the pipeline for chronic hepatitis B virus(HBV)*** has also been a significant emphasis on screening and surveillance programs,widespread availability of vaccines,and linkage of *** these efforts,significant gaps persist in care,and there is a pressing need for increased collaboration and teamwork across the globe to achieve a reduction of disease burden and elimination of HBV and HcV.

关键词： Viral hepatitis Recent advances Novel therapies Barriers to cure Future direction