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Inter-Laboratory Ring Trial to evaluate Reverse Transcription Polymerase Chain Reaction Methods Used for <i>Dolphin Morbillivirus</i>Detection in Italy

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Advances in Microbiology 2019年第5期9卷 423-437页

作者： Zoccola Roberto Pautasso Alessandra Grattarola Carla Monnier Milena Miceli Ilaria Ingravalle Francesco Crescio Maria Ines Giorda Federica Masoero Loretta Zaccaria Guendalina Purpari Giuseppa Cersini Antonella Viscardi Maurizio Serracca Laura Padalino Iolanda Puggioni Giantonella Canonico Cristina Toffan Anna Centelleghe Cinzia Di Francesco Cristina esmeralda Mazzariol Sandro Di Guardo Giovanni Mignone Walter Casalone Cristina Goria Maria istituto zooprofilattico sperimentale del Piemonte Liguria e Valle d’Aosta Torino Italy istituto zooprofilattico sperimentale dell’Abruzzo e del Molise Teramo Italy istituto zooprofilattico sperimentale della Sicilia Palermo Italy istituto zooprofilattico sperimentale del Lazio e della Toscana Roma Italy istituto zooprofilattico sperimentale del Mezzogiorno Portici (NA) Italy istituto zooprofilattico sperimentale del Piemonte Liguria e Valle d’Aosta La Spezia Italy istituto zooprofilattico sperimentale della Puglia e Basilicata Foggia Italy istituto zooprofilattico sperimentale della Sardegna Sassari Italy istituto zooprofilattico sperimentale dell’ umbria e delle marche Ancona Italy istituto zooprofilattico sperimentale delle Venezie Legnaro (PD) Italy University of Padova Department of Comparative Biomedicine and Food Science (BCA) Legnaro (PD) Italy Faculty of Veterinary Medicine University of Teramo Teramo Italy istituto zooprofilattico sperimentale del Piemonte Liguria e Valle d’Aosta Imperia Liguria Italy

Dolphin Morbillivirus (DMV) is one of the most frequently detected pathogens in stranded cetacean specimens worldwide as well as in Italy. Due to the persistence of DMV in the Mediterranean Sea and to the lack of information about the efficiency of the available diagnostic techniques, the Italian National Reference Centre for diagnostic activities on dead stranded marine mammals (***) performed the first inter-laboratory ring trial with the aim to standardize a diagnostic biomolecular approach for DMV in Italy. Viral isolation is usually considered the “gold standard” for the definitive diagnosis of most pathogens, but it is not often feasible in DMV diagnosis, due to the poor preservation of virus-targeted tissues in stranded cetacean carcasses, as well as to the lack of appropriate sensitivity of cell lines towards DMV variability. Therefore direct viral detection on tissues by means of reverse transcription-PCR (RT-PCR) represents a valuable option for DMV infection’s diagnosis. For detecting DMV in cetacean die-offs occurred in the Mediterranean basin since 2013, *** developed an RT-PCR based method targeting to a 287 bp fragment of DMV nucleoprotein (N) gene. With the purpose to evaluate its performances in terms of accuracy (Se = sensitivity and Sp = specificity) and precision (reproducibility), it was submitted to a ring trial. So, 12 Public Laboratories belonging to the Italian dead stranded marine mammals diagnostic network were asked to analyze a panel of 40 samples (positive and negative for DMV, using different dilutions of a viral suspension obtained from a cell culture supernatant of a DMV strain) with the aforementioned technique. Furthermore, we also aimed at comparing the accuracy of other 7 molecular methods routinely applied for DMV detection in Italy. For this purpose, the second panel of identical 40 DMV +ve and -ve samples was provided to Laboratories that routinely used DMV detection methods other than those developed by ***.

关键词： Dolphin Morbillivirus RT-PCR Interlab Ring Test Method Comparison Stranded Cetaceans Mediterranean Sea

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Some pathogenic characters of paratyphoid Salmonella enterica strains isolated from poultry

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Asian Pacific Journal of Tropical Medicine 2017年第12期10卷 1161-1166页

作者： Fabrizio Bertelloni Giovanni Tosi Paola Massi Laura Fiorentini Maria Parigi Domenico Cerri Valentina Virginia ebani Department of Veterinary Science University of PisaViale Delle Piagge 256124 Pisa Italy istituto zooprofilattico sperimentale della Lombardia e dell'emilia Romagna Sezione Diagnostica di Forl(i)Via Don E. Servadei 3E/3F47122 Forl(i)(FC) Italy

Objective: To investigate some pathogenic characters of Salmonella enterica strains isolated from ***: Twenty-three genetically distinct Salmonella enterica strains, of different serovars and pulsotype, were examined for virulence traits. Resistance to gastric acid environment was estimated by measuring the percentage of survived bacterial cells after exposure for 2 h to a synthetic gastric juice. Strains were analyzed with PCR for the presence of the following virulence genes: mgtC and rhu M located on SPI-3, sopB and pipB located on SPI-5, Salmonella virulence plasmid(spv)R(spvR), spvB and spvC located on Salmonella plasmid virulence and sodCI, sope, and gipA located on ***, resistance to 21 antibiotics was tested with Kirby–Bauer ***: A percentage of 82.60% of strains were resistant to gastric environment after induction and 60.87% of the strains exhibited constitutive resistance too. Nineteen different virulence profiles were detected. The phage related genes sodCI and sope and the plasmid mediated operon spvR, spvB and spvC(spvRBC) were detected in 82.60%,47.82% and 52.17% of strains, respectively. Typhimurium and enteritidis strains showed the highest number of virulence genes. Twenty-one different antibiotic resistance profiles were obtained and two isolates(Typhimurium and enteritidis) resulted sensible to all the tested molecules. The ampicillin, streptomycin, sulfonamide and tetracycline resistance profile was detected in seven isolates(30.43%).Conclusion: Our results show that paratyphoid Salmonella strains with several characters of pathogenicity, that may be cause of severe pathology in animals and humans, are circulating among poultry.

关键词： Paratyphoid salmonellae Poultry Gastric acid resistance Virulence genes Antimicrobial resistance

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effect of dietary supplementation with yeast cell wall extracts on performance and gut response in broiler chickens

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Journal of Animal Science and Biotechnology 2020年第3期11卷 736-746页

作者： A.Pascual M.Pauletto M.Giantin G.Radaelli C.Ballarin M.Birolo C.Zomeño M.Dacasto M.Bortoletti M.Vascellari G.Xiccato A.Trocino Department of Comparative Biomedicine and Food Science(BCA) University of PadovaViale dell’Università16I-35020 LegnaroPadovaItaly department of agronomy foodnatural resourcesanimaland environment(dafnae)university of padovaviale dell’università16i-35020 legnaropadovaitaly histopathology department istituto zooprofilattico sperimentale delle venezieviale dell’università10i-35020 legnaropadovaitaly

Background:The dietary supplementation of yeast cell wall extracts(YCW)has been found to reduce pathogenic bacteria load,promote immunoglobulin production,prevent diseases by pro-inflammatory responses,and alter gut microbiota *** study evaluated growth and slaughter results,health,gut morphology,immune status and gut transcriptome of 576 male chickens fed two diets,i.e.C(control)or Y(with 250–500 g/t of YCW fractions according to the growth period).At 21 and 42 d the jejunum of 12 chickens per diet were sampled and stained with hematoxylin/eosin for morphometric evaluation,with Alcian-PAS for goblet cells,and antibodies against CD3+intraepithelial T-cells and CD45+intraepithelial *** jejunum sampled at 42 d were also used for wholetranscriptome ***:Dietary YCW supplementation did not affect final live weight,whereas it decreased feed intake(114 to 111 g/d;P≤0.10)and improved feed conversion(1.74 to 1.70;P≤0.01).Regarding the gut,YCW supplementation tended to increase villi height(P=0.07);it also increased the number of goblet cells and reduced the density of CD45+cells compared to diet C(Pe gut transcriptome,four genes were expressed more in broilers fed diet Y compared to diet C,*** P450,family 2,subfamily C,polypeptide 23b(CYP2C23B),tetratricopeptide repeat domain 9(TTC9),basic helix-loop-helix family member e41(BHLHe41),and the metalloreductase *** one gene set(HeS_PATHWAY)was significantly enriched among the transcripts more expressed in broilers fed diet ***,a total of 41 gene sets were significantly over-represented among genes up-regulated in control ***,several enriched gene sets are implicated in immune functions and related to NF-κB signaling,apoptosis,and interferon ***:The dietary YCW supplementation improved broiler growth performance,increased gut glycoconjugate secretion and reduced the inflammatory status together with differences in the gut tran

关键词： Gut morphometry Immuno-histochemistry Saccharomyces cerevisiae Transcriptomic analysis

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efficacy of four commercial disinfectants against H5N1 highly pathogenicity avian influenza virus

Efficacy of four commercial disinfectants against H5N1 highl...

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The 15th World Veterinary Poultry Congress

作者： C.Terregino P.De Benedictis e.Bertoli M.S.Beato I.Capua OIe FAO and National Reference Laboratory for Avian Influenza and Newcastle DiseaseIstituto Zooprofilattico Sperimentale delle VenezieViale dell’Università 1035020LegnaroPadovaItaly OIe FAO and National Reference Laboratory for Avian Influenza and Newcastle DiseaseIstituto Zooprofilattico Sperimentale delle VenezieViale dell’Università 1035020LegnaroPadovaItaly OIe FAO and National Reference Laboratory for Avian Influenza and Newcastle DiseaseIstituto Zooprofilattico Sperimentale delle VenezieViale dell’Università 1035020LegnaroPadovaItaly OIe FAO and National Reference Laboratory for Avian Influenza and Newcastle DiseaseIstituto Zooprofilattico Sperimentale delle VenezieViale dell’Università 1035020LegnaroPadovaItaly OIe FAO and National Reference Laboratory for Avian Influenza and Newcastle DiseaseIstituto Zooprofilattico Sperimentale delle VenezieViale dell’Università 1035020LegnaroPadovaItaly

＜正＞Introduction Avian Influenza（AI）represents one of the greatest concerns for public health that has emerged from the animal reservoir in recent *** ongoing H5N1 epidemics have led to devastating consequences fo...

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