Effect of dietary supplementation with yeast cell wall extracts on performance and gut response in broiler chickens

作     者：A.Pascual M.Pauletto M.Giantin G.Radaelli C.Ballarin M.Birolo C.Zomeño M.Dacasto M.Bortoletti M.Vascellari G.Xiccato A.Trocino A. Pascual;M. Pauletto;M. Giantin;G. Radaelli;C. Ballarin;M. Birolo;C. Zome?o;M. Dacasto;M. Bortoletti;M. Vascellari;G. Xiccato;A. Trocino

作者机构：Department of Comparative Biomedicine and Food Science(BCA)University of PadovaViale dell’Università16I-35020 LegnaroPadovaItaly department of agronomyfoodnatural resourcesanimaland environment(dafnae)university of padovaviale dell’università16i-35020 legnaropadovaitaly histopathology departmentistituto zooprofilattico sperimentale delle venezieviale dell’università10i-35020 legnaropadovaitaly 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2020年第11卷第3期

页      面：736-746页

核心收录：

学科分类：090502[农学-动物营养与饲料科学] 0905[农学-畜牧学] 09[农学] 

基　　金：The project was funded by Lesaffre(C26C18000320007) 

主　　题：Gut morphometry Immuno-histochemistry Saccharomyces cerevisiae Transcriptomic analysis 

摘      要：Background:The dietary supplementation of yeast cell wall extracts(YCW)has been found to reduce pathogenic bacteria load,promote immunoglobulin production,prevent diseases by pro-inflammatory responses,and alter gut microbiota *** study evaluated growth and slaughter results,health,gut morphology,immune status and gut transcriptome of 576 male chickens fed two diets,i.e.C(control)or Y(with 250–500 g/t of YCW fractions according to the growth period).At 21 and 42 d the jejunum of 12 chickens per diet were sampled and stained with hematoxylin/eosin for morphometric evaluation,with Alcian-PAS for goblet cells,and antibodies against CD3+intraepithelial T-cells and CD45+intraepithelial *** jejunum sampled at 42 d were also used for wholetranscriptome ***:Dietary YCW supplementation did not affect final live weight,whereas it decreased feed intake(114 to 111 g/d;P≤0.10)and improved feed conversion(1.74 to 1.70;P≤0.01).Regarding the gut,YCW supplementation tended to increase villi height(P=0.07);it also increased the number of goblet cells and reduced the density of CD45+cells compared to diet C(P0.001).In the gut transcriptome,four genes were expressed more in broilers fed diet Y compared to diet C,*** P450,family 2,subfamily C,polypeptide 23b(CYP2C23B),tetratricopeptide repeat domain 9(TTC9),basic helix-loop-helix family member e41(BHLHE41),and the metalloreductase *** one gene set(HES_PATHWAY)was significantly enriched among the transcripts more expressed in broilers fed diet ***,a total of 41 gene sets were significantly over-represented among genes up-regulated in control ***,several enriched gene sets are implicated in immune functions and related to NF-κB signaling,apoptosis,and interferon ***:The dietary YCW supplementation improved broiler growth performance,increased gut glycoconjugate secretion and reduced the inflammatory status together with differences in the gut tran