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Terpenoid Indole Alkaloids Biosynthesis and Metabolic engineering in Catharanthus roseus

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Journal of Integrative Plant Biology 2007年第7期49卷 961-974页

作者： Dong-Hui Liu Hong-Bin Jin Yu-Hui Chen Li-Jie Cui Wei-Wei Ren Yi-Fu Gong Ke-Xuan Tang Shanghai Key Laboratory of Agrobiotechnology Plant Biotechnology Research Center Fudan-Shanghai Jiaotong University ( SJTU)-Nottingham Plant Biotechnology R & D Center School of Agriculture and Biology Shanghai Jiaotong University Shanghai 200030 China Department of Biochemistry and Molecular Biology Shenyang Medical College Shenyang 110034 China Faculty of Life Sciences and biotechnology Ningbo University Ningbo 315211 China State Key Laboratory of genetic engineering School of Life Sciences Fudan-SJTU-Nottingham Plant Biotechnology R & D Center Morgan-Tan International Center for Life Sciences Fudan University Shanghai 200433 China

Catharanthus roseus L. （Madagascar periwinkle） biosynthesizes a diverse array of secondary metabolites including anticancer dimeric alkaloids （vinblastine and vincristine） and antihypertensive alkaloids （ajmalicine and serpentine）. The multi-step terpenoid indole alkaloids （TIAs） biosynthetic pathway in C. roseus is complex and is under strict molecular regulation. Many enzymes and genes involved in the TIAs biosynthesis have been studied in recent decades. Moreover, some regulatory proteins were found recently to control the production of TIAs in C. roseus. Based on mastering the rough scheme of the pathway and cloning the related genes, metabolic engineering of TIAs biosynthesis has been studied in C. roseus aiming at increasing the desired secondary metabolites in the past few years. The present article summarizes recent advances in isolation and characterization of TIAs biosynthesis genes and transcriptional regulators involved in the second metabolic control in C. roseus. Metabolic engineering applications in TIAs pathway via overexpression of these genes and regulators in C. roseus are also discussed.

关键词： biosynthesis pathway Catharanthus roseus metabolic engineering terpenoid indole alkaloids.

Introgression of Drought Tolerance into Elite Basmati Rice Variety through Marker-Assisted Backcrossing

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Phyton-international Journal of Experimental Botany 2023年第5期92卷 1421-1438页

作者： Muhammad Sabar Shahzad Amir Naveed Shahid Masood Shah Abdul Rehman Khan Muhammad Musaddiq Shah Tahir Awan Muhammad Ramzan Khan Zaheer Abbas Muhammad Arif National Institute for biotechnology and genetic engineering(NIBGE) Faisalabad38000Pakistan Rice Research Institute Kala Shah Kaku39020Pakistan National Institute for Genomics and Advanced biotechnology(NIGAB) Islamabad44000Pakistan Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing100081China Department of biotechnology COMSATS University IslamabadAbbottabad22020Pakistan Department of biotechnology University of SialkotSialkot51310Pakistan Pakistan Institute of engineering and Applied Sciences Islamabad44000Pakistan

Drought is one of the major abiotic threat to rice production in the context of climate *** Basmati is an elite,fine grain basmati rice variety grown in Punjab,*** to drought sensitive in nature,its yield has been facing an alarming situation in production because of gradual decrease in irrigated water for a couple of *** reported novel QTLs for drought tolerance were selected for incorporation into Super Basmati by employing marker assisted selection ***55419-04 with novel QTLs was used as a donor *** selection was performed by applying PCR based QTL linked SSR markers followed by recombinant selection by using 2-4 flanking *** selection was exercised by using polymorphic SSR markers for maximum genome recovery of the Super *** individuals homozygous at the target QTLs and with maximum background of Super Basmati at the rest of the non-target genome was selected for evaluation of drought *** drought stress conditions,the yields of all introgressed lines(ILs)were 44.2%-125.7%higher than recurrent *** superior ILs that are drought tolerant and very similar to Super Basmati in terms of agronomic and grain quality traits are marked for release as drought-tolerant varieties in arid regions or for use in breeding programs of high grain quality and drought-tolerant parents.

关键词： Drought QTL SSR markers basmati rice drought marker assisted breeding

Production of γ-linolenic acid and stearidonic acid by Synechococcus ***7002 containing cyanobacterial fatty acid desaturase genes

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Chinese Journal of Oceanology and Limnology 2016年第4期34卷 772-780页

作者：董学卫何庆芳彭振英于金慧边斐李有志毕玉平 College of Life Science and Technology State Key Laboratory for Conservation and Utilization of Subtropical Agro- Bioresources Guangxi University biotechnology Research Center Shandong Academy of Agricultural Science Key Laboratory for Genetic Improvement of Crop Animal and Poultry of Shandong Province Key Laboratory of Crop Genetic Improvement and Biotechnology Huanghuaihai Ministry of Agriculture Department of Applied Science University of Arkansas

genetic modifi cation is useful for improving the nutritional qualities of cyanobacteria. To increase the total unsaturated fatty acid content, along with the ratio of ω-3/ω-6 fatty acids, genetic engineering can be used to modify fatty acid metabolism. S ynechococcus sp. PCC7002, a fast-growing cyanobacterium, does not contain a Δ6 desaturase gene and is therefore unable to synthesize γ-linolenic acid(GLA) and stearidonic acid(SDA), which are important in human health. In this work, we constructed recombinant vectors Syd6 D, Syd15 D and Syd6Dd15 D to express the Δ15 desaturase and Δ6 desaturase genes from Synechocystis PCC6803 in Synechococcus sp. PCC7002, with the aim of expressing polyunsaturated fatty acids. Overexpression of the Δ15 desaturase gene in S ynechococcus resulted in 5.4 times greater accumulation of α-linolenic acid compared with the wild-type while Δ6 desaturase gene expression produced both GLA and SDA. Co-expression of the two genes resulted in low-level accumulation of GLA but much larger amounts of SDA, accounting for as much to 11.64% of the total fatty acid content.

关键词： Synechococcus sp.PCC7002 Synechocystis sp.PCC6803 Δ15 fatty acid desaturase Δ6 fatty acid desaturase polyunsaturated fatty acids

Biological and Functional Properties of Wedelolactone in Human Chronic Diseases

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Phyton-international Journal of Experimental Botany 2021年第1期90卷 1-15页

作者： Ramachandran Vinyagam Pradeep Kumar Kyung Eun Lee Baojun Xu Muhammad Nurul Matin Sang Gu Kang Department of biotechnology Institute of BiotechnologyCollege of Life and Applied ScienceYeungnam UniversityGyeongsanGyeongbuk38541Korea Department of Forestry North Eastern Regional Institute of Science and TechnologyNirjuli791109India Stemforce 313 Institute of Industrial TechnologyYeungnam UniversityGyeongsanGyeongbuk38541Korea Food Science and Technology Program Beijing Normal University-Hong Kong Baptist UniversityUnited International CollegeZhuhai519000China Department of genetic engineering and biotechnology University of RajshahiRajshahi6205Bangladesh

Medicinal herbs are well known and studied over the past millennia in most of the developing countries as a rational means of treatment against various diseases and ***(WDL),a major bioactive compound in Eclipta prostrata L(Eclipta alba L),has been reported with potential benefits in human health against chronic ***,a comprehensive study on WDL pharmacological benefits in various ailments,to the best of our knowledge,is not yet ***,the present review provides the recent therapeutic applications in reference to biological and functional activities against major human chronic diseases,including cardiovascular,cancer,diabetes mellitus,liver disease,Alzheimer’s disease,and androgenetic *** this study,we collected all the relevant experimental information on WDL from Scientific databases such as PubMed,Web of Science,Science Direct,and Google ***,WDL is recognized as a key anti-oxidant with both specific regulator and inhibitor of major drug targetable proteins in human chronic diseases and ***,WDL as a novel therapeutic bioactive molecule is advised to explore further for relevant pharmacological activities.

关键词： Wedelolactone Eclipta alba L Ecliptaprostrata L bioactivity bioavailability

Prevalence and Resistance Pattern of <i>Pseudomonas aeruginosa</i>Isolated from Surface Water

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Advances in Microbiology 2015年第1期5卷 74-81页

作者： Marufa Nasreen Animesh Sarker M. A. Malek Md. Ansaruzzaman Mahububur Rahman Department of biotechnology and genetic engineering Mawlana Bhashani Science and Technology University Santosh Tangail Bangladesh Department of Microbiology University of Dhaka Dhaka Bangladesh international centre for Diarrheal Diseases Research Dhaka Bangladesh

Pseudomonas aeruginosa is one of the most common pathogenic bacteria, frequently found in different environmental samples. The prevalence of multidrug resistant isolates has become an alarming concern for both patients and their surroundings. The present study was carried out to record prevalence of P. aeruginosa in surface water of Dhaka city and to screen their antibiotic resistance pattern. The study was also extended to typing of resistant isolates according to extended spectrum beta lactamase production. Hereby, Kirby-Bauer method was applied to test antibiotic sensitivity according to Clinical and Laboratory Standards Institute. Then, the Ampicillin resistant isolates were screened for ESBL production by Double Disk Synergy Test (DDST). In these prospects, 52 water samples were tested, of which 32 were found positive for P. aeruginosa isolates. Hundred percent of the positive isolates were found to Ampicillin (AMP) resistant followed by 93.7% to both Tetracycline and Gentamycin and 71.8% to Co-triimoxazole. P. aeruginosa is completely susceptible to third generation antibiotics ciprofloxacin, Imipenem and Aztreonam followed by moderately susceptible to Polymyxin-B (78.2%) and Colistin (87.5%). According to DDST, all of the susceptible isolates were found positive for AMC type beta-lactamase production. It is evident from this study that the surface water is contaminated with antibiotic resistant P. aeruginosa and that through the water systems antibiotic resistance can be transferred to humans and animals. So, appropriate and rationale use of antibiotic should be applied to minimize the emergence of multidrug isolates to environment.

关键词： P. aeruginosa Beta-Lactamase DDST Multidrug Resistant

Glycoproteomics analysis of plasma proteins associated with Opisthorchis viverrini infection-induced cholangiocarcinoma in hamster model

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Asian Pacific Journal of Tropical Medicine 2016年第12期9卷 1142-1147页

作者： Pramote Sriwanitchrak Atchara Paemanee Sittiruk Roytrakul Vithoon Viyanant Kesara Na-Bangchang Graduate Program in Biomedical Sciences Faculty of Allied Health SciencesThammasat University Proteomics Research Laboratory National Center for Genetic Engineering and Biotechnology Graduate Program in Bioclinical Sciences Center of Excellence in Pharmacology and Molecular Biology of Malaria and CholangiocarcinomaChulabhorn International College of Medicine Center of Excellence in Pharmacology and Molecular Biology of Malaria and Cholangiocarcinoma Chulabhorn International College of Medicine Excellence Center in Pharmacology and Molecular Biology of Malaria and Cholangiocarcinoma Thammasat University

Objective: To apply lectin affinity chromatography and glycoproteomics-based LC-MS/MS to preliminarily investigate the possible potential plasma biomarkers of Opisthorchis viverrini(OV)-associated CCA in OV/dimethylnitrosamine(DMN)-induced CCA hamster model. Methods: Nine Syrian hamsters were divided into 3 groups as follows(n=3 each): normal(healthy control group); OV group; and OV/DMN group(CCA group). Pooled plasma samples collected from animals in each group at the 6th month post-infection with OV metacercarae were subjected to glycoproteomics analysis. Glycoproteins in the pooled sample from each group were initially isolated by concanavilin A(Con A)-based affinity chromatography. The expression of glycoproteins isolated by both enrichment methods were determined using LCMS/MS. Results: Among the 24 Con A-binding glycoproteins isolated, two proteins, N-myc downstream regulated gene 1(NDRG1) and fetuin-B(FETUB) were found up-regulated only in the samples from the OV and control groups, but not in the OV/DMN(CCA) groups. On the other hand, one protein, i.e., NSFL1 cofactor p47 isoform x3(NSFL1C) was found only in the samples from OV/DMN(CCA) and control groups, but not in the OV group. The remaining 21 proteins were upregulated in the samples from all groups. Conclusions: NDRG1, FETUB and NSFL1 C glycoproteins isolated by Con A-based affinity chromatography could be potential biomarkers for CCA. Plasma samples with negative for NDRG1 and FETUB proteins but positive for NSFL1 C are likely to be OV-associated CCA. Nevertheless, this conclusion remains to be confirmed whether this battery test can discriminate OV-associated CCA from other risk factors.

关键词： Cholangiocarcinoma Opisthorchis viverrini Glycoprotein Hamster model ConA binding protein LC-MS/MS

Clinical Significance of Apurinic/Apyrimidinic Endodeoxyribonuclease 1 and MicroRNA Axis in Hepatocellular Carcinoma

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Journal of Clinical and Translational Hepatology 2023年第6期11卷 1291-1307页

作者： Giovanna Mangiapane Devis Pascut Emiliano Dalla Giulia Antoniali Monica Degrassi Lory Saveria Crocè Veronica De Sanctis Silvano Piazza Giulia Canarutto Claudio Tiribelli Gianluca Tell Laboratory of Molecular Biology and DNA Repair Department of MedicineUniversity of UdineUdineItaly Fondazione Italiana Fegato-ONLUS Liver Cancer UnitTriesteItaly Department of Medical Sciences University of TriesteTriesteItaly Clinica Patologie Fegato Azienda Sanitaria Universitaria Giuliano Isontina(ASUGI)TriesteItaly NGS Core Facility Department CIBIOUniversity of TrentoTrentoItaly Computational Biology International Centre for Genetic Engineering and BiotechnologyICGEBTriesteItaly

Background and Aims:Identification of prognostic factors for hepatocellular carcinoma(HCC)opens new perspectives for *** and cellular onco-miRNAs are noncoding RNAs which can control the expression of genes involved in oncogenesis through post-transcriptional *** microRNAs(miRNAs)are considered novel prognostic and predictive factors in *** apurinic/apyrimidinic endodeoxyribonuclease 1(APE1)contributes to the quality control and processing of specific onco-miRNAs and is a negative prognostic factor in several *** present work aims to:a)define APE1 prognostic value in HCC;b)identify miRNAs regulated by APE1 and their relative target genes and c)study their prognostic ***:We used The Cancer Genome Atlas(commonly known as TCGA)data analysis to evaluate the expression of APE1 in *** identify differentially-expressed miRNAs(DEmiRNAs)upon APE1 depletion through specific small interfering RNA,we used NGS and nanostring approaches in the JHH-6 HCC tumor cell *** analyses were performed to identify signaling pathways involving APE1-regulated *** analysis was performed to identify miRNAs correlating with serum APE1 ***:APE1 is considerably overexpressed in HCC tissues compared to normal liver,according to the TCGA-liver HCC(known as LIHC)*** analyses showed that APE1-regulated miRNAs are implicated in signaling and metabolic pathways linked to cell proliferation,transformation,and angiogenesis,identifying Cyclin Dependent Kinase 6 and Lysosomal Associated Membrane Protein 2 as ***-33a-5p,miR-769,and miR-877 are related to lower overall survival in HCC *** array profiling,we identified eight circulating DE-miRNAs associated with APE1 overexpression.A training phase identified positive association between sAPE1 and miR-3180-3p and ***:APE1 regulates specific miRNAs having prognostic value in HCC.

关键词： APE1 miRNAs Prognostic factors Hepatocellular carcinoma

genetic Diversity,Population Structure,and Genome-Wide Association Study of Seven Agronomic Traits in 273 Diverse Upload Cotton Accessions

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Phyton-international Journal of Experimental Botany 2023年第12期92卷 3345-3357页

作者： Yajun Liang Juyun Zheng Junduo Wang Zhaolong Gong Zhiqiang Li Ling Min Zeliang Zhang Zhiwei Sang Yanying Qu Xueyuan Li Quanjia Chen Cash Crops Research Institute of Xinjiang Academy of Agricultural Science(XAAS) UrumqiChina engineering Research centre of Cotton of Ministry of Education Xinjiang Agricultural UniversityUrumqiChina R&D Department Adsen Biotechnology CorporationUrumqiChina National Key Laboratory of Crop genetic Improvement Huazhong Agricultural UniversityWuhanChina

Upland cotton(Gossypium hirsutum)is the most important plant producing natural fibers for the textile *** this study,we first investigated the phenotypic variation of seven agronomic traits of 273 diverse cotton accessions in the years 2017 and 2018,which were from 18 geographical *** found large variations among the traits in different geographical regions and only half of the traits in either years 2017 or 2018 followed a normal *** then genotyped the collection with 81,612 high quality *** tree and population structure revealed a diverse genetic structure of the core collection,and geographical diversification was an important factor,but account for part of the variances of genetic *** then performed genome-wide association study for the seven traits in the years 2017 and 2018,and the average values of each trait in the two years,*** identified a total of 19 significant marker-trait associations and found that Pollen Ole e 1 allergen/extension could be the candidate gene associated with the fall-off cotton bolls from the last three *** addition,large variations were observed for the heritability of traits in the years 2017 and *** results provide new potential candidate genes for further functional validation,which could be useful for genetic improvement and breeding of new cotton cultivars with better agronomic performances.

关键词： Upland cotton agronomic trait genetic diversity population structure genome-wide association study

Expression of HIV-1 env Glycoprotein gp120 and gp160 in Vaccinia Virus System and Their Antigenicity Analysis

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Chinese Medical Sciences Journal 1997年第2期12卷 132-132页

作者：陈元鼎 Rodolfo Garcia Sergio Tisminetzky 郭仁 Francisco E.Baralle Institute of Medical Biology CAMS & PUMC Kunming 650107 International Centre for Genetic Engineering and Biotechnology UNIDO Trieste 34012 Italy international centre for genetic engineering and biotechnology UNIDOTrieste 34012 Italy Institute of Medical Biology CAMS & PUMC Kunming 650107

Human immunodeficiency virus type 1 (HIV-1)is the principal causative agent of AIDS which is threatening human health. The most important antigen of HIV1 is the viral envelope glycoprotein, gp160. The mature form of the HIV-1 gp160 is made up of two major regions:the extracellular (gq120) and the transmembrane (gp41) domains. The gp120 binds with high affinity to CD4. he cellular receptor for HIV-1, and antibodies that prevent the CD4-gq120 interaction are able to block HIV-1 infection. A principal neutralizing deter-

关键词： HIV-1 抗原 gp160 牛痘病毒糖蛋白基因表达艾滋病