Production of γ-linolenic acid and stearidonic acid by Synechococcus ***7002 containing cyanobacterial fatty acid desaturase genes

作     者：董学卫 何庆芳 彭振英 于金慧 边斐 李有志 毕玉平 

作者机构：College of Life Science and Technology State Key Laboratory for Conservation and Utilization of Subtropical Agro- Bioresources Guangxi University Biotechnology Research Center Shandong Academy of Agricultural Science Key Laboratory for Genetic Improvement of Crop Animal and Poultry of Shandong Province Key Laboratory of Crop Genetic Improvement and Biotechnology Huanghuaihai Ministry of Agriculture Department of Applied Science University of Arkansas 

出 版 物：《Chinese Journal of Oceanology and Limnology》 (中国海洋湖沼学报（英文版）)

年 卷 期：2016年第34卷第4期

页      面：772-780页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：Supported by the International S&T Cooperation Program of China(No.2012DFA30450) the National Natural Science Foundation of China(No.30871541) the Taishan Scholar Foundation of Shandong Province(No.tshw20091014) the Innovation Program of the University Institutes of Jinan,Shandong Province(No.201004044) 

主　　题：Synechococcus sp.PCC7002 Synechocystis sp.PCC6803 Δ15 fatty acid desaturase Δ6 fatty acid desaturase polyunsaturated fatty acids 

摘      要：Genetic modifi cation is useful for improving the nutritional qualities of cyanobacteria. To increase the total unsaturated fatty acid content, along with the ratio of ω-3/ω-6 fatty acids, genetic engineering can be used to modify fatty acid metabolism. S ynechococcus sp. PCC7002, a fast-growing cyanobacterium, does not contain a Δ6 desaturase gene and is therefore unable to synthesize γ-linolenic acid(GLA) and stearidonic acid(SDA), which are important in human health. In this work, we constructed recombinant vectors Syd6 D, Syd15 D and Syd6Dd15 D to express the Δ15 desaturase and Δ6 desaturase genes from Synechocystis PCC6803 in Synechococcus sp. PCC7002, with the aim of expressing polyunsaturated fatty acids. Overexpression of the Δ15 desaturase gene in S ynechococcus resulted in 5.4 times greater accumulation of α-linolenic acid compared with the wild-type while Δ6 desaturase gene expression produced both GLA and SDA. Co-expression of the two genes resulted in low-level accumulation of GLA but much larger amounts of SDA, accounting for as much to 11.64% of the total fatty acid content.