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Proline and Oxidative Metabolism in young Pecan Trees Associated with Sulphate Accumulation

Phyton-International Journal of Experimental Botany 2022年第6期91卷 1141-1152页

作者： Dalila Jacqueline Escudero-Almanza Oscar Cruz-Alvarez Ofelia Adriana Hernández-Rodríguez Juan Luis Jacobo-Cuellar Esteban Sánchez-Chávez Pablo Preciado-Rángel Dámaris Leopoldina Ojeda-Barrios Facultad de ciencias Agrotecnológicas Universidad Autónoma de ChihuahuaChihuahua31350México Centro de investigación en Alimentación y desarrollo A.C.Delicias33088México institutotecnologico nacional de México-instituto Tecnológico de Torreón Coahuila27170México

Pecan[Carya illinoinensis(Wangenh.)***.]is a deciduous tree whose fruits(nuts)are of high economic value and offer excellent nutritional ***,soils high in sulphates can limit its growth and *** with 5-year-old trees of‘Western Schley’pecan grown in soils high in sulphates,the levels of proline and oxidative metabolism were recorded in the *** showed that different levels of visible leaflet damage(‘sufficiency’,‘low’,‘moderate’or‘severe’)were associated with different levels of leaflet sulphates(mg kg^(−1)):‘sufficiency’(≤40),‘low’(41–60),‘moderate’(61–80)and‘severe’(80–100).‘Severe’sulphate damage was associated with significant reductions in chlorophyll(TChl)(17.04μg g^(−1)),relative water content(RWC)(50%)and leaf area(LA),and with increases in the concentrations of total carotenoids(TC)and proline(Prl).Increases were also observed in the activities of the oxidative metabolism enzymes:superoxide dismutase(SOD)(1.82 units min^(−1) g^(−1)),catalase(CAT)(2.86μmol H_(2)O_(2) min^(−1) g^(−1))and antioxidant capacity(AC)(87%DPPH inhibition).However,guaiacol peroxidase(GP)showed a reduction(2.97 nmol GSH min^(−1) g^(−1)).An inverse relationship was found between the sulphate concentration in the leaflets with respect to the evaluated parameters of TChl,TC,RWC,LA,AC,and *** synthesis and antioxidant enzymatic activity indicate salt stress in pecan leaflets in orchards irrigated with deep-well water high in sulphates.

关键词： Carya illinoinensis catalase guaiacol peroxidase salt stress photosynthetic pigments superoxide dismutase

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Dietary carbohydrate-to-protein ratio influences growth performance,hepatic health and dynamic of gut microbiota in atlantic salmon(Salmo salar)

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Animal nutrition 2022年第3期10卷 261-279页

作者： Alejandro Villasante Carolina Ramírez Hector Rodríguez Patricio Dantagnan Adrian Hernandez Elías Figueroa Jaime Romero Facultad de Medicina Veterinaria y Agronomía Universidad de Las AmericasSantiagoChile Laboratorio de Biotecnología de Alimentos Unidad de AlimentosInstituto de Nutricion y Tecnología de Los Alimentos(INTA)Universidad de ChileSantiagoChile Programa de Anatomía y Biología del desarrollo Facultad de MedicinaUniversidad de ChileSantiagoChile núcleo de investigacion en Produccion Alimentaria Facultad de Recursos NaturalesUniversidad Catolica de TemucoTemucoChile

Atlantic salmon(Salmo salar)fed a carbohydrate-rich diet exhibit suboptimal growth performance,along with other metabolic *** is well known that gut microbes play a pivotal role in influencing metabolism of the host,and these microbes can be modified by the *** main goal of the present study was to determine the effect of feeding graded levels of digestible carbohydrates to Atlantic salmon on the distal intestine digesta microbiota at 3 sampling times(i.e.,weeks 4,8 and 12),during a 12-week trial.A low carbohydrate-to-high protein diet(LC/HP,0%wheat starch),a medium carbohydrate-tomedium protein diet(MC/MP,15%wheat starch)or a high carbohydrate-to-low protein diet(HC/LP,30%wheat starch)was fed to triplicate fish tanks(27 to 28 fish per tank).We performed an in-depth characterization of the distal intestine digesta ***,growth parameters,liver histology and the expression of genes involved in hepatic neolipogenesis in fish were *** fed a HC/LP diet showed greater hepatosomatic and viscerosomatic indexes(P=0.026 and P=0.018,respectively),lower final weight(P=0.005),weight gain(P=0.003),feed efficiency(P=0.033)and growth rate(P=0.003)compared with fish fed the LC/HP ***,feeding salmon a high digestible carbohydrate diet caused greater lipid vacuolization,steatosis index(P=0.007)and expression of fatty acid synthase(fas)and delta-6 fatty acyl desaturase(d6fad)(P=0.001 and P=0.001,respectively)in the liver compared with fish fed the LC/HP ***,the major impact of feeding a carbohydrate-rich diet to Atlantic salmon in beta diversity of distal intestine digesta microbiota was observed at week 4(HC/LP vs MC/MP and HC/LP vs LC/HP;P=0.007 and P=0.008,respectively)and week 8(HC/LP vs MC/MP;P=0.04),no differences between experimental groups were detected after 12 weeks of ***,at the end of the trial,there was a negative correlation between lactic acid bacteria(LAB)members,including Leuconostoc and Lactobacil

关键词： Microbiota Microbiome Atlantic salmon Steatosis Lactic acid bacteria Probiotics

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Soil organic carbon stocks in native forest of Argentina:a useful surrogate for mitigation and conservation planning under climate variability

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Ecological Processes 2024年第1期13卷 3-24页

作者： Pablo L.Peri Juan Gaitán Matías Mastrangelo Marcelo nosetto Pablo E.Villagra Ezequiel Balducci Martín Pinazo Roxana P.Eclesia Alejandra Von Wallis Sebastián Villarino Francisco Alaggia Marina González Polo Silvina Manrique Pablo A.Meglioli Julián Rodríguez-Souilla Martín Mónaco Jimena E.Chaves Ariel Medina Ignacio Gasparri Eugenio Alvarez Arnesi María Paula Barral Axel von Müller norberto M.Pahr Josefina Uribe Echevarria Pedro Fernández Marina Morsucci Dardo López Juan Manuel Cellini Leandro Alvarez Ignacio Barberis Hernán Colomb Ludmila La Manna Sebastián Barbaro Cecilia Blundo Ximena Sirimarco Laura Cavallero Gualberto Zalazar Guillermo Martínez Pastur instituto nacional de tecnología Agropecuaria (InTA) Universidad nacional de la Patagonia Austral (UnPA) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Universidad nacional de Luján Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Grupo de Estudio de Agroecosistemas y Paisajes Rurales (GEAP) Universidad Nacional de Mar del Plata Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET ) CCT San Luis Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) instituto Argentino de nivología Glaciología y Ciencias Ambientales (IANIGLA) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Universidad Nacional de Cuyo instituto de investigaciones en Biodiversidad y Medioambiente (InIBIOMA) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) instituto de investigaciones en energía no Convencional CCT Salta-JujuyConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Laboratorio de Recursos Agroforestales Centro Austral de Investigaciones Científicas (CADIC) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Dirección nacional de Bosques Ministerio de Ambiente y Desarrollo Sostenible de la Nación instituto de Ecología Regional Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET )Universidad Nacional de Tucumán Universidad nacional de Rosario Instituto de Investigaciones en Ciencias Agrarias de Rosario Centro Científico Tecnológico CONICET Grupo de Estudio de Agroecosistemas y Paisajes Rurales (GEAP) Universidad Nacional de Mar del Plata Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Laboratorio de investigaciones en Maderas (LIMAD) Universidad Nacional de La Plata (UNLP) Administración de Parques nacionales (APn) Parque Nacional Los Alerces Centro de Estudios Ambientales Integrados (CEAI) Universidad Nacional de la Patagonia San Juan Bosco (UNPASJB) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET )

Background The nationally determined contribution（nDC） presented by Argentina within the framework of the Paris Agreement is aligned with the decisions made in the context of the United nations Framework Conven-tion on Climate Change（UnFCCC） on the reduction of emissions derived from deforestation and forest degradation, as well as forest carbon conservation（REDD+）. In addition, climate change constitutes one of the greatest threats to forest biodiversity and ecosystem services. However, the soil organic carbon（SOC） stocks of native forests have not been incorporated into the Forest Reference Emission Levels calculations and for conservation planning under cli-mate variability due to a lack of information. The objectives of this study were:（i） to model SOC stocks to 30 cm of native forests at a national scale using climatic, topographic and vegetation as predictor variables, and（ii） to relate SOC stocks with spatial–temporal remotely sensed indices to determine biodiversity conservation concerns due to threats from high inter-annual climate *** We used 1040 forest soil samples（0–30 cm） to generate spatially explicit estimates of SOC native forests in Argentina at a spatial resolution of approximately 200 m. We selected 52 potential predictive environmental covari-ates, which represent key factors for the spatial distribution of SOC. All covariate maps were uploaded to the Google Earth engine cloud-based computing platform for subsequent modelling. To determine the biodiversity threats from high inter-annual climate variability, we employed the spatial–temporal satellite-derived indices based on enhanced Vegetation Index（EVI） and land surface temperature（LST） images from Landsat *** SOC model（0–30 cm depth） prediction accounted for 69% of the variation of this soil property across the whole native forest coverage in Argentina. Total mean SOC stock reached 2.81 Pg C（2.71–2.84 Pg C with a probability of 90%） for a total area of 460,790 km2,

关键词： Soil organic carbon Biodiversity native forest Climate change mitigation Modelling Resilience

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neuroprotective effect of immunomodulatory peptides in rats with traumatic spinal cord injury

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neural Regeneration Research 2021年第7期16卷 1273-1280页

作者： Dulce Parra-Villamar Liliana Blancas-Espinoza Elisa Garcia-Vences Juan Herrera-García Adrian Flores-Romero Alberto Toscano-Zapien Jonathan Vilchis Villa Rodríguez Barrera-Roxana Soria Zavala Karla Antonio Ibarra Raúl Silva-García Unidad de investigación Médica en Inmunología Hospital de PediatríaCentro Médico Nacional Siglo XXI/Instituto Mexicano del Seguro Social/Ciudad de MéxicoMéxico Centro de investigación en ciencias de la Salud(CICSA) Facultad de Ciencias de la SaludUniversidad Anáhuac MéxicoCampus NorteHuixquilucanEdo de MéxicoMéxico Centro de investigación del Proyecto Camina A.C Ciudad de MéxicoMéxico

Several therapies have shown obvious effects on structural conservation contributing to motor functional recovery after spinal cord injury(SCI).nevertheless, neither strategy has achieved a convincing *** purposed a combined therapy of immunomodulatory peptides that individually have shown significant effects on motor functional recovery in rats with *** objective of this study was to investigate the effects of the combined therapy of monocyte locomotion inhibitor factor(MLIF), A91 peptide, and glutathione monoethyl ester(GSH-MEE) on chronic-stage spinal cord *** Sprague-Dawley rats underwent a laminectomy of the T9 vertebra and a moderate *** groups were included: sham, PBS, MLIF + A91, MLIF + GSH-MEE, A91 + GSH-MEE, and MLIF + A91 + *** months after injury, motor functional recovery was evaluated using the open field *** and white matter preservation was evaluated using hematoxylin & eosin staining and Luxol Fast Blue staining, *** number of motoneurons in the ventral horn and the number of axonal fibers were determined using hematoxylin & eosin staining and immunohistochemistry, *** deposition was evaluated using Masson's trichrome *** combined therapy of MLIF, A91, and GSH-MEE greatly contributed to motor functional recovery and preservation of the medullary parenchyma, white matter, motoneurons, and axonal fibres, and reduced the deposition of collagen in the lesioned *** combined therapy of MLIF, A91, and GSH-MEE preserved spinal cord tissue integrity and promoted motor functional recovery of rats after *** study was approved by the national Commission for Scientific Research on Bioethics and Biosafety of the instituto Mexicano del Seguro Social under registration number R-2015-785-116(approval date november 30, 2015) and R-2017-3603-33(approval date June 5, 2017).

关键词： glutathione monoethyl ester monocyte locomotion inhibitor factor motor functional recovery neuroprotection neurorestoration peptides protective autoimmunity spinal cord injury

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Wound healing activity of Ullucus tuberosus,an Andean tuber crop

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Asian Pacific Journal of Tropical Biomedicine 2017年第6期7卷 538-543页

作者： nathalie Heil Karent Bravo Andrés Montoya Sara Robledo Edison Osorio Grupo de investigación en Sustancias Bioactivas Facultad de Ciencias Farmacéuticas y AlimentariasUniversidad de Antioquia PECET–Medical Research Institute School of MedicineUniversity of Antioquia

Objective:This study was designed to investigate the wound healing activity of aqueous extracts of Ullucus tuberosus(***) using in vitro ***:Lyophilized pulp and acetone extracts of *** were produced using ultrasound *** capacity for collagenase activation was evaluated using fluorescence detection of the enzymatic ***,the influence of *** extracts on cell proliferation,cell migration and synthesis of the extracellular matrix(ECM)proteins,metalloproteinase(MMP-1) and pro-collagen was analyzed using human dermal fibroblasts in ***:An increase in collagenase activity of 12% supports the utility of *** as an agent for scar *** addition,the extracts showed an increase in the proliferation and migration of human dermal fibroblasts and the production of pro-collagen and MMP-1 after treatment with *** *** increase in proliferation,migration and pro-collagen levels positively influenced the regeneration of scarless tissue during the proliferation phase,whereas the increase in MMP-1 may have favored the wound healing process during the remodeling and cellular differentiation ***:The results of this study show for first time that *** is a promising candidate to support scarless tissue regeneration.

关键词： Wound healing Collagenase Pro-collagen MMP-1 Ullucus tuberosus

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Polyurethane Composites Synthesized Using natural Oil-Based Polyols and Sisal Fibe

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Journal of Renewable Materials 2018年第4期6卷 426-437页

作者： S.Michalowski M.A.Mosiewicki M.Kuran´ska M.I.Aranguren A.Prociak department of Chemistry and Technology of Polymers Faculty of Chemical Engineering and TechnologyCracow University of TechnologyWarszawska 2431-155CracowPoland instituto de investigaciones en ciencia y tecnología de Materiales-Universidad nacional de Mar del Plata Consejo Nacional de Investigaciones Científicas y TécnicasAv.J.B.Justo 43027600Mar del PlataArgentina

Elastomeric polyurethanes were prepared from a reference polyurethane system modified with biobased polyols synthesized using rapeseed or palm *** reference material was modified by replacement of the commercial polyol by 10%of biopolyols and also by addition of sisal fibers up to 5 wt%.The higher functionality of the biopolyols increased the crosslinking density of the networks and this was reflected by an increase in hardness and a decrease in water *** effect of the sisal fibers mainly improved the mechanical and thermomechanical properties of the system with rapeseed oil because of good dispersion and strong fiber-matrix *** system containing palm oil polyol corresponded to an initial formulation of high viscosity and,consequently,the resulting composites presented lower densities than the theoretically expected *** results are associated with fiber dispersion problems in the initial reactive mixture.

关键词： Biopolyols palm oil rapeseed oil polyurethane composites

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Leishmaniasis in the Argentine Republic: Temporal and geographical distribution from 2013 to 2017

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Asian Pacific Journal of Tropical Medicine 2019年第7期12卷 300-305页

作者： GermanóMaría J Salomón María C neira Gisela Lozano Esteban Mackern-Oberti Juan P Cargnelutti Diego E Consejo nacional de investigaciones Científicas y Técnicas Centro Científico y Tecnológico de MendozaInstituto de Medicina y Biología Experimental de CuyoMendozaArgentina Universidad nacional de Cuyo Facultad de Ciencias Médicasárea de ParasitologíaMendozaArgentina Universidad Juan Agustín Maza Facultad de Ciencias Veterinarias y AmbientalesCentro de Investigación en Parasitología RegionalMendozaArgentina Universidad nacional de Cuyo Facultad de Ciencias MédicasInstituto de FisiologíaMendozaArgentina

Objective: To assess the temporal and geographical distribution of confirmed cases of cutaneous, mucocutaneous and visceral leishmaniasis in the Argentine Republic from 2013 to 2017. Methods: A retrospective study was carried out using data collected from the Integrated Surveillance Bulletin database of the national System of Health Surveillance. Confirmed cases of cutaneous, mucocutaneous and visceral leishmaniasis up to the 52 nd epidemiological week of each year was included. Results: In the 5 years period, 1 295 confirmed leishmaniasis cases were reported in the Argentine Republic. One thousand twenty-eight(1 028) cases corresponded to cutaneous leishmaniasis(87.10%), being the most common type of leishmaniasis. Mucocutaneous leishmaniasis was in the second place in the country with 115 cases reported, mostly in the northwest and northeast regions. A total of 52 individuals with visceral leishmaniasis were identified and Misiones Province was the most affected. Conclusions: It is important to analyze the temporal and geographical distribution of leishmaniasis in order to provide an adequate management and surveillance.

关键词： Leishmaniasis Epidemiology Argentine Republic Temporal distribution Geographical distribution

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Digestibility,Antioxidant and Anti-Inflammatory Activities of Pecan nutshell(Carya illioinensis)Extracts

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Journal of Renewable Materials 2022年第10期10卷 2569-2580页

作者： María Janeth Rodríguez-Roque Carmen Lizette del-Toro-Sánchez Janet Madeline Chávez-Ayala Ricardo Iván González-Vega Liliana Maribel Pérez-Pérez Esteban Sánchez-Chávez nora AideéSalas-Salazar Juan Manuel Soto-Parra Rey David Iturralde-García María Antonia Flores-Córdova Facultad de ciencias Agrotecnológicas Universidad Autónoma de ChihuahuaChihuahuaMéxico Universidad de Sonora Rosales y Niños Héroes S/NHermosilloSonMéxico Centro de investigación en Alimentación y desarrollo A.C.Unidad DeliciasDeliciasChihuahuaMéxico

Phenolic compounds are related to high biological activity,avoiding oxidation in food and human *** are by-products derived from the pecan nut processing that contain important amounts of phenols which biological activity must be *** research aimed to evaluate the antioxidant(DPPH,ABTS,FRAP and hemolysis)and anti-inflammatory activities of shell extracts from pecan nuts harvested during the crop production cycle 2018 and 2019,as well as the in vitro digestibility of their phenolic compounds,including *** showed that extracts from the crop production cycle 2018 obtained the highest yield,while those from 2019 contained the highest concentration of phenolic compounds,flavonoids and antioxidant capacity determined by DPPH(22.96 mmol ET),ABTS(91.55 mmol ET)and inhibition of hemolysis(92.12%).The anti-inflammatory activity exhibited an inhibition of the elastase enzyme up to 50 min and the bioaccessibility of phenolic compounds reached up to 32%.These results showed that pecan nutshell extracts are an important source of biologically active compounds,thus,they are suitable to be used as commodities in different fields such as agricultural,food and pharmaceutical *** studies must be carried out in order to elucidate the activity of nutshell extracts within in vivo systems.

关键词： Anti-inflammatory activity antioxidants digestibility shell phenolic compounds

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Multi-temporal nDVI analysis using UAV images of tree crowns in a northern Mexican pine-oak forest

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Journal of Forestry Research 2023年第6期34卷 1855-1867页

作者： JoséLuis Gallardo-Salazar Marcela Rosas-Chavoya Marín Pompa-García Pablito Marcelo López-Serrano Emily García-Montiel Arnulfo Meléndez-Soto Sergio Iván Jiménez-Jiménez instituto de investigaciones Agropecuarias y Forestales Universidad Michoacana de San Nicolás de Hidalgo(UMSNH)58330 MoreliaMichoacánMexico Programa Institucional de Doctorado en ciencias Agropecuarias y Forestales Universidad Juárez del Estado de DurangoRío Papaloapan y Blvd.DurangoValle del Sur s/n34120 DurangoDurangoMéxico Facultad de ciencias Forestales Universidad Juárez del Estado de DurangoRío Papaloapan y Blvd.DurangoValle del Sur s/n34120 DurangoDurangoMéxico instituto de Silvicultura e Industria de la Madera Universidad Juárez del Estado de DurangoBoulevard del Guadiana 501Ciudad UniversitariaTorre de Investigación34120 DurangoMéxico InIFAP-CenID RASPA Centro nacional de investigación Disciplinaria en Relación Agua-Suelo-Planta-Atmósfera Margen derecha canal Sacramento km 6.5Zona industrial35140 Gómez PalacioDurangoMexico

The use of unmanned aerial vehicles(UAV)for forest monitoring has grown significantly in recent years,providing information with high spatial resolution and temporal *** with multispectral sensors allow the use of indexes such as the normalized difference vegetation index(nDVI),which determines the vigor,physiological stress and photo synthetic activity of *** study aimed to analyze the spectral responses and variations of nDVI in tree crowns,as well as their correlation with climatic factors over the course of one *** study area encompassed a 1.6-ha site in Durango,Mexico,where Pinus cembroides,Pinus engelmannii,and Quercus grisea *** images were acquired with UAV and information on meteorological variables was obtained from nASA/POWER *** AnOVA explored possible differences in nDVI among the three *** correlation was performed to identify the linear relationship between nDVI and meteorological *** differences in nDVI values were found at the genus level(Pinus and Quercus),possibly related to the physiological features of the species and their *** grisea had the lowest nDVI values throughout the year which may be attributed to its sensitivity to relative humidity and *** the use of UAV with a multispectral sensor for nDVI monitoring allowed genera differentiation,in more complex forest analyses hyperspectral and LiDAR sensors should be integrated,as well other vegetation indexes be considered.

关键词： Multispectral images normalized diff erence Vegetation index Phenology Unmanned aerial vehicles Multitemporal analysis

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Explicit Relations of Physical Potentials Through Generalized Hypervirial and Kramers' Recurrence Relations

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Communications in Theoretical Physics 2015年第6期63卷 682-686页

作者：孙国华董世海 Catedra COnACyT Centro de Investigacion en ComputacionInstituto Politecnico Nacional departamento de Fisica Escuela Superior de Ffsica y MatematicasInstituto Politecnico Nacional

Based on a Hamfltonian identity, we study one-dimensional generalized hypervirial theorem, Blanchardlike （non-diagonal case） and Kramers＇（diagonal case） recurrence relations for arbitrary x^k which is independent of the central potential V（x）. Some significant results in diagonal case are obtained for special k in xk （k ≥2）. In particular, we find the orthogonal relation 〈n1｜n2〉 = δh1,n2 （k = 0）, 〈n1[V＇（x）｜n2〉 = （en1-en2）^2〈n1｜x｜n2〉（k = 1）, en = （n｜V＇（x）x/2｜n〉＋（n｜V（x）｜n〉（k = 2） and -4en（n｜x｜n） ~ 〈n｜V＇（x）x^2｜n〉＋ 4〈n｜V（x）x｜n〉 =0 （k=3）. The latter two formulas can be used directly to calculate the energy levels. We present useyul explicit relations for some well known physical potentials without requiring the energy spectra of quantum system.

关键词： Hamiltonian identity hypervirial relations Kramers' recurrence relation physical potentials

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