检索结果-南通市图书馆

Paternal physical exercise modulates global DNA methylation status in the hippocampus of male rat offspring

Neural Regeneration Research 2019年第3期14卷 491-500页

作者： Christiano Spindler ethiane Segabinazi Andre Luis Ferreira de Meireles Francele Valente Piazza Filipe Mega Gabriela dos Santos Salvalaggio Matilde Achaval Viviane Rostirola elsner Simone Marcuzzo Programa de Pos-Graduacao em Neurociencias Instituto de Ciencias Basicas da Saude Universidade Federal do Rio Grande do Sul Laboratorio de Histofisiologia Comparada Departamento de Ciencias Morfologicas Instituto de Ciencias Basicas da Saude Universidade Federal do Rio Grande do Sul Programa de Pos-Graduacao em Biociências e Reabilitacao Centro Universitario Metodista-IPA

It is widely known that maternal physical exercise is able to induce beneficial improvements in offspring cognition; however, the effects of paternal exercise have not been explored in detail. The present study was designed to evaluate the impact of paternal physical exercise on memory and learning, neuroplasticity and DNA methylation levels in the hippocampus of male offspring. Adult male Wistar rats were divided into two groups: sedentary or exercised fathers. The paternal preconception exercise protocol consisted of treadmill running, 20 minutes daily, 5 consecutive days per week for 22 days, while the mothers were not trained. After mating, paternal sperm was collected for global DNA methylation analysis. At postnatal day 53, the offspring were euthanized, and the hippocampus was dissected to measure cell survival by 5-bromo-2′-deoxiuridine and to determine the expression of synaptophysin, reelin, brain-derived neurotrophic factor and global DNA methylation levels. To measure spatial memory and learning changes in offspring, the Morris water maze paradigm was used. There was an improvement in spatial learning, as well as a significant decrease in hippocampal global DNA methylation levels in the offspring from exercised fathers compared with those from sedentary ones; however, no changes were observed in neuroplasticity biomarkers brain-derived neurotrophic factor, reelin and 5-bromo-2′-deoxiuridine. Finally, the global DNA methylation of paternal sperm was not significantly changed by physical exercise. These results suggest a link between paternal preconception physical activity and cognitive benefit, which may be associated with hippocampal epigenetic programming in male offspring. However, the biological mechanisms of this modulation remain unclear.

关键词： epigenetics preconception methylation learning treadmill running fetal programming inheritance sperm nerve regeneration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Role of sex hormones in gastrointestinal motility in pregnant and non-pregnant rats

引用

World Journal of Gastroenterology 2016年第25期22卷 5761-5768页

作者： Juliana Fernandes Matos Madileine Francely Americo Yuri Karen Sinzato Gustavo Tadeu Volpato Luciana Aparecida Corá Marcos Felipe Freitas Calabresi Ricardo Brandt Oliveira debora Cristina damasceno Jose Ricardo Arruda Miranda Sao Paulo State University BotucatuSP 18618-970Brazil instituto de ciencias biologicas e da saude Federal University of Mato GrossoBarra do GarcasMT 78600-000Brazil Universidade estadual de ciencias da saude de Alagoas MaceioAL 57010-300Brazil Sao Paulo University Ribeirao PretoSP 14049-900Brazil

AIM: To correlate gastric contractility, gastrointestinal transit, and hormone levels in non-pregnant(estrous cycle) and pregnant rats using noninvasive techniques. MeTHODS: Female rats(n = 23) were randomly divided into(1) non-pregnant,(contractility, n =6; transit, n = 6); and(2) pregnant(contractility, n = 5; transit, n = 6). In each estrous cycle phase or at 0, 7, 14, and 20 d after the confirmation of pregnancy, gastrointestinal transit was recorded by AC biosusceptometry(ACB), and gastric contractility was recorded by ACB and electromyography. After each recording, blood samples were obtained for progesterone and estradiol determination. ReSULTS: In the estrous cycle, despite fluctuations of sex hormone levels, no significant changes in gastrointestinal motility were observed. days 7 and 14 of pregnancy were characterized by significant changes in the frequency of contractions(3.90 ± 0.42 cpm and 3.60 ± 0.36 cpm vs 4.33 ± 0.25 cpm) and gastric emptying(168 ± 17 min and 165 ± 15 min vs 113 ± 15 min) compared with day 0. On these same days, progesterone levels significantly increased compared with control(54.23 ± 15.14 ng/m L and 129.96 ± 30.52 ng/mL vs 13.25 ± 6.31 ng/mL). On day 14, we observed the highest level of progesterone and the lowest level of estradiol compared with day 0(44.3 ± 15.18 pg/mL vs 24.96 ± 5.96 pg/mL). CONCLUSION: Gastrointestinal motility was unaffected by the estrous cycle. In our data, high progesterone and low estradiol levels can be associated with decreased contraction frequency and slow gastric emptying.

关键词： Reproductive physiological process Sex hormones Gastrointestinal motility Magnetic fields Gastric emptying

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Candida albicans isolated from urine:Phenotypic and molecular identification,virulence factors and antifungal susceptibility

引用

Asian Pacific Journal of Tropical Biomedicine 2017年第7期7卷 624-628页

作者： Laura Wiebusch Adriana Araújo de Almeida-Apolonio Luana Mireli Carbonera Rodrigues Bruna de Paula Bicudo danielly Beraldo dos Santos Silva danielle Ferreira Lonchiati Renata Pires de Araujo Alexéia Barufatti Grisolia Kelly Mari Pires de Oliveira Faculdade de ciencias biologicas e Ambientais Universidade Federal da Grande DouradosDouradosMSBrazil Faculdade de Medicina Universidade Federal de Mato Grosso do SulCampo GrandeMSBrazil Faculdade de ciencias da saude Universidade Federal da Grande DouradosDouradosMSBrazil Faculdade de ciencias Agrarias e Veterinarias Universidade Estadual Paulista“Julio de Mesquita Filho”JaboticabalSPBrazil

Objective: To isolate Candida albicans(C. albicans) from the urine of hospitalized patients and assess the virulence factors and antifungal susceptibility profiles of the ***: Yeasts were identified using the chromogenic medium CHROMagar?, the VITeK~?2 system, hypertonic Sabouraud broth, tobacco agar, polymerase chain reaction,and DNA sequencing. The evaluated virulence factors were proteinase production,phospholipase production, and biofilm production on polystyrene. The broth microdilution technique was used to determine the minimum inhibitory ***: All yeasts isolated from urine were identified as C. albicans using both classical and molecular methods. Although 91.3% of the isolates showed no phospholipase activity, 56.5% showed strong proteinase activity and 91.7% produced biofilm. All microorganisms were sensitive to fluconazole, voriconazole and amphotericin B, but 56.5% of the yeasts showed resistance to ***: C. albicans isolates from urine have a high capacity for virulence and can be associated with infectious processes. Furthermore, the high percentage of isolates resistant to itraconazole is important because this antifungal agent is commonly used to treat fungal infections in the hospital environment.

关键词： Candidiasis Urinary tract Infection Virulence Antifungal susceptibility

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Protein profile of leprosy patients with plantar ulcers from the eastern Amazon region

引用

Infectious Diseases of Poverty 2017年第1期6卷 947-954页

作者： Marineia Porto de Oliveira Jorge Rodrigues de Sousa Rafael Silva de Araujo Tinara Leila de Sousa Aarao Juarez Antonio Simoes Quaresma Nucleo de Medicina Tropical Universidade Federal do ParaAv.Generalissimo Deodoro 92UmarizalBelemPara 66055240Brazil Centro de ciencias biologicas e da saude Universidade do Estado do ParaBelemParaBrazil

Background:Studies investigating the nutritional status of patients with leprosy and plantar ulcers are ***,the objective of this study was to describe the protein profile of leprosy patients with plantar ulcers from the eastern Amazon ***:A case record form was created for 75 patients with leprosy(31 with plantar ulcers and 44 without plantar ulcers)with the following data:sociodemographic characteristics,clinical form of leprosy,presence or absence of plantar ulcers,and nutritional assessment using anthropometry consisting of the measurement of body mass index,arm circumference,arm muscle circumference,and triceps *** of blood albumin,transferrin,and C-reactive protein(CRP)were also *** regarding protein intake were obtained using a Food Frequency ***:Plantar ulcers occurred more frequently in male patients(67.7%),patients aged 40-49 years(mean±SD:47.3±8.0 years),and patients receiving 300 or 600 USD(71.0%).The mean weight and height of patients were 71.6±11.4 kg and 1.62±0.1 m,*** levels of CRP were detected in 51.6%of leprosy patients with plantar ulcers and only 9.1%of patients without plantar ulcers(Pdepletion of transferrin was observed in 14.3%of patients with paucibacillary leprosy and 44.3%of patients with multibacillary leprosy(P=0.0447).Most patients had normal levels of serum albumin(74.2%with plantar ulcers and 77.3%without plantar ulcers).Conclusions:Most leprosy patients with plantar ulcers have normal levels of serum albumin and transferrin and high CRP levels,which indicates the presence of an inflammatory *** findings suggest the need to monitor patients with leprosy to prevent the occurrence of plantar ulcers and to provide adequate treatment for patients with existing plantar ulcers.

关键词： Leprosy Plantar ulcer Nutritional status Anthropometric assessment Albumin Transferrin C-reactive protein

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Rat Cerebrospinal Fluid Treatment Method through Cisterna Cerebellomedullaris Injection

引用

Neuroscience Bulletin 2018年第5期34卷 827-832页

作者： Thaina Garbino dos Santos Mery Stefani Leivas Pereira Diogo Losch Oliveira Laboratory of Cellular Neurochemistry Departamento de Bioquimica Instituto de Ciencias Basicas da Saude Universidade Federal do Rio Grande do Sul Porto Alegre RS 90035-003 Brazil

Drugs that lack the ability to cross the blood- brain barrier （BBB） need to be placed directly into the central nervous system. Our laboratory studies the involve- ment of the glutamatergic system in the aggressiveness of glioma, and some ligands of glutamate receptors cannot permeate the BBB. Here, glioma-implanted rats were treated by a technique that delivers ligands directly into the cerebrospinal fluid by puncture into the cisterna cerebel- lomedullaris. Rats were anesthetized and fixed in a rodent stereotactic device. The head was gently tilted downwards at an angle that allowed exposure of the cisterna. Injection into the cisterna was done freehand using a gingival needle coupled to a microsyringe. The efficiency of intracisternal injection was demonstrated using a methylene blue solu- tion. This type of injection is adaptable for any rodent model using small volumes of a variety of other drugs, and is an interesting method for neuroscience studies.

关键词： Neurobiology Neuroscience Drug administration Intracisternal injection Cisterna magna Cerebrospinal fluid treatment Central nervous system Surgical technique Rodent

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The neuroprotective effect of traditional Chinese medicinal plants--A critical review

引用

Acta Pharmaceutica Sinica B 2023年第8期13卷 3208-3237页

作者： Joao Moreira Mariana Machado Monica Dias-Teixeira Ricardo Ferraz Cristina delerue-Matos Clara Grosso ReQUIMTe/LAQV Instituto Superior de Engenharia do PortoInstituto Politecnico do PortoPorto 4249-015Portugal ciencias Químicas e das Biomoleculas/CISA Escola Superior de SaudedInstituto Politecnico do PortoPorto 4200-072Portugal NICiTeS-Nucleo de Investigacao em ciencias e Tecnologias da saude Escola Superior de Saude Ribeiro SanchesLisboa 1950-396Portugal ReQUIMTe/LAQV Departamento de Química e Bioquímica Faculdade de CienciasUniversidade do PortoPorto 4169-007Portugal

Neurodegenerative and neuropsychiatric diseases are increasingly affecting individuals’quality of life,thus increasing their cost to social and health *** diseases have overlapping mechanisms,such as oxidative stress,protein aggregation,neuroinflammation,neurotransmission impairment,mitochondrial dysfunction,and ***,there is no cure for neurodegenerative diseases,and the available therapies have adverse effects and low *** neuropsychiatric disorders,such as depression,the current therapies are not adequate to one-third of the patients,the so-called treatmentresistant ***,searching for new treatments is *** plants appear as a strong alternative and complement towards new treatment protocols,as they have been used for health purposes for thousands of ***,the main goal of this review is to revisit the neuroprotective potential of some of the most predominant medicinal plants(and one fungus)used in traditional Chinese medicine(TCM),focusing on their proven mechanisms of action and their chemical compositions,to give clues on how they can be useful against neurodegeneration progression.

关键词： Neurodegeneration Alzheimer's disease Parkinson's disease depression Medicinal plants Oxidative stress Toxicology Clinicaltrials

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A prospective cancer chemo-immunotherapy approach mediated by synergistic CD326 targeted porous silicon nanovectors

引用

Nano Research 2015年第5期8卷 1505-1521页

作者： Mohammad-Ali Shahbazi Neha Shrestha ermei Makila Francisca Araujo Alexandra Correia Tomas Ramos Bruno Sarmento Jarno Salonen Jouni Hirvonen Helder A. Santos Division of Pharmaceutical Chemistry and Technology Faculty of Pharmacy University ofHelsinki FI-00014 Helsinki Finland Laboratory of Industrial Physics Departmen t of Physics and Astronomy University of Turku FI-20014 Turku Finland INeB - instituto de engenharia Biomedica NewTherapies Group Universidade do Porto Rua do Campo Alegre 823 4150-180 Porto Portuga ICBAS - instituto ciencias Biomedicas Abel Salazar University of Porto Rua de Jorge Viterbo Ferreira 4050-313 Porto Portugal Faculty of Pharmacy University of Coimbra 3000-548 Coimbra Portugal IINFACTS - instituto de Investigacao e Formacao Avancada em ciencias e Tecnologias da saude Instituto Superior de Ciencias da Saude-Norte Department of Pharmaceutical Sciences CESPU Rua Central de Gandra 4585-116 Gandra Portugal

Combination therapy via nanoparticulate systems has already been proposed as a synergistic approach for cancer treatment. Herein, undecylenic acid modified thermally hydrocarbonized porous silicon nanoparticles （UnTHCPSi NPs） loaded with sorafenib and surface-biofunctionalized with anti-CD326 antibody （Ab） were developed for cancer chemo-immunotherapy in MCF-7 and Mda-MB-231 breast cancer cells. The cytocompatibility study showed no significant toxicity for the bare and antibody-conjugated UnTHCPSi （Un-Ab） NPs at concentrations lower than 200 μg·mL^-1. Compared to the bare UnTHCPSi, Un-Ab NPs loaded with sorafenib reduced the premature drug release in plasma, increasing the probability of proper drug targeting. In addition, high cellular interaction and subsequent internalization of the Un-Ab NPs into the cells expressing CD326 antigen demonstrated the possibility of improving antigen-mediated endocytosis via CD326 targeting. While an in vitro antitumor study revealed a higher inhibitory effect of the sorafenib-loaded Un-Ab NPs compared to the drug-loaded UnTHCPSi NPs in the CD326 positive MCF-7 cells, there was no difference in the anti-proliferation impact of both the abovementioned NPs in the CD326 negative Mda-MB-231 cells, suggesting CD326 as an appropriate receptor for Ab-mediated drug delivery. It was also shown that the anti-CD326 Ab can act as an immunotherapeutic agent by inducing antibody dependent cellular cytotoxicity and enhancing the interaction of effector immune and cancer cells for subsequent phagocytosis and cytokine secretion. Hence, the developed nanovectors can be applied for simultaneous tumor-selective drug targeting and immunotherapy.

关键词： CD326 antibody porous silicon nanopartides chemo-immunotherapy breast cancer drug targeting

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

All-electron basis sets for H to Xe specific for ZORA calculations:Applications in atoms and molecules

引用

Chinese Physics B 2024年第8期33卷 238-246页

作者： C.S.Gomes F.e.Jorge A.Canal Neto departamento de Fisica Universidade Federal do Espirito Santo29075-910 VitoriaESBrazil departamento de Fisica Instituto de Ciencias Exatas e BiologicasUniversidade Federal de Ouro Preto Campus Universitario Morro do Cruzeiro35402-136 Ouro PretoMGBrazil

A segmented basis set of quadruple zeta valence quality plus polarization functions(QZP)for H through Xe was developed to be used in conjunction with the ZORA *** set was augmented with diffuse functions to describe electrons farther away from the nuclei *** the ZORA-CCSD(T)/QZP-ZORA theoretical model,atomic ionization energies and bond lengths,harmonic vibrational frequencies,and atomization energies of some molecules were *** addition of core-valence corrections has been shown to improve the agreement between theoretical and experimental results for molecular *** atomization energies,a similar observation emerges when considering spin-orbit *** the augmented QZP-ZORA set,static mean dipole polarizabilities of a set of atoms were calculated and compared with previously published recommended and experimental *** evaluations of the ZORA and Douglas–Kroll–Hess Hamiltonians were made for each property studied.

关键词： QZP-ZORA and AQZP-ZORA basis sets elements from H to Xe CCSD(T)method atomic and molecular properties

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Queens remate despite traumatic mating in stingless bees

引用

Current Zoology 2022年第1期68卷 81-92页

作者： Jamille Costa VeIGA Gustavo Rodrigo Sanches RUIZ Gislene Almeida CARVALHO-ZILSe Cristiano MeNeZeS Felipe Andres Leon CONTReRA Bee Biology and ecology Lab Instituto de Ciencias BiologicasUniversidade Federal do ParaBelem66075-110 ParaBrazil Biodiversity and ecosystem Services Research Group Instituto Tecnologico Vale-Desenvolvimento SustentavelBelem66055-090 ParaBrazil ecology and Zoology of Invertebrates Lab Instituto de Ciencias BiologicasUniversidade Federal do ParaBelem66075-110 ParaBrazil Bee Research Group Instituto Nacional de Pesquisas da AmazoniaManaus69067-375 AmazonasBrazil Research and development Embrapa Meio AmbienteJaguariuna13820-000 Sao PauloBrazil

Males can control female reproduction using genital plugs to impede access by *** social bees,ants,and wasps,plugging may involve traumatic mating,with females being *** stingless bees,chances are that plugs may promote ovarian activan,and are thought to ensure single mating—a general tendency among the social ***,understanding on relationships between mating plugs,traumatic mating,and mating systems in stingless bees remains *** address this,we(1)compared mated queens of 7 Neotropical species to understand the patterns of copulatory marks in females and(2)compared pre-and post-mating genitalia of males and females in Melipona fasciculata to depict plug functional *** revealed an unprecedented consequence of mating in stingless bees:the characteristic marks left by mating plugs on female abdomens and the inferences that can be made from *** our surprise,in 1 species *** we found that queens retain the plug long after mating,and may carry it for the rest of their *** the other 6 species retained the plug for only a short *** queens were only found in ***,whose multiple copulatory marks match previous findings of polyandry in this *** study shows that queens can remate,and suggests that male genital morphology may determine in part the time persistence of *** conclude that traumatic mating plugs do not fully prevent remating in stingless bees and that mating systems are not uniform in this ***,exceptional cases of facultative polyandry in social insects—for example,when mating plugs fail—may confirm a general tendency for single mating in close link with efficient mating plugs.

关键词： copulatory marks facultative polyandry genital morphology male harm sexual conflict

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Lower marine productivity increases agonistic interactions between sea lions and fur seals in Northern Pacific Patagonia

引用

Current Zoology 2022年第6期68卷 657-666页

作者： Felipe MONTALVA Hector PAVeS Diego PeReZ-VeNeGAS Karin G BARRIeNTOS e Carola VALeNCIA Diego MIRANda-URBINA Mauricio SeGUeL Facultad de ciencias biologicas Pontificia Universidad Catolica de ChileSantiagoChile departamento de ciencias Basicas Facultad de CienciasUniversidad Santo TomasOsornoChile Centro de Investigacion y Gestion de Recursos Naturales(CIGReN) Instituto de BiologıaFacultad de CienciasUniversidad de ValparaısoValparaısoChile Programa de Doctorado en ciencias Agrarias Escuela de Graduados de laFacultad de Ciencias Agrarias y AlimentariasUniversidad Austral de ChileValdiviaChile Facultad de Medicina Veterinaria Sede PatagoniaUniversidad San SebastianPuerto MonttChile instituto de ciencias biologicas Universidad de TalcaTalcaChile department of Pathobiology Ontario Veterinary CollegeUniversity of GuelphGuelphCanada

Interspecific interactions are key drivers of individual and population-level fitness in a wide range of ***,in marine ecosystems,it is relatively unknown which biotic and abiotic factors impact behavioral interactions between competing *** assessed the impact of weather,marine productivity,and population structure on the behavioral agonistic interactions between South American fur seals(SAFSs),Arctocephalus australis,and South American sea lions(SASLs),Otaria byronia,in a breeding colony of *** hypothesized that agonistic interactions between SAFSs and SASLs respond to biotic and abiotic factors such as SAFS population structure,marine productivity,and *** found that SASL and SAFS interactions almost always resulted in negative impacts on the social structure or reproductive success of the SAFS *** adult males initiated stampedes of SAFS and/or abducted and predated SAFS *** SAFS males abundance and severe weather events were negatively correlated with agonistic interactions between ***,proxies for lower marine productivity such as higher sea surface temperature and lower catches of demerso-pelagic fish were the most important predictors of more frequent agonistic interactions between SAFS and *** the current scenario of decline in marine biomass due to global climate change and overfishing,agonistic interactions between competing marine predators could increase and exacerbate the negative impacts of environmental change in these species.

关键词： interspecific interactions climate change Guafo Island sea surface temperature South American fur seal South American sea lion

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：