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the 20GD Superfamily： Emerging Functions in Plant Epigenetics and Hormone Metabolism

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Molecular Plant 2018年第10期11卷 1222-1224页

作者： Riad Nadi Eduardo Mateo-Bonmati Lucia Juan-Vicente Jose Luis Micol instituto de bioingenieria Universidad Miguel Hern&ndez Campus de Elche 03202 Elche Spain

The 2-oxoglutarate and Fe （ll）-dependent dioxygenase （2OGD） superfamily includes oxidative enzymes with an active site con- taining two histidines and （in most cases） one aspartic or glutamic acid residue. This conserved motif is termed the 2-His-1-carboxylate facial triad, chelates iron, and is housed within a double-stranded β-helix fold, also known as the DSBH, jelly-roll, cupin, or Jumonji C fold （Martinez and Hausinger, 2015）. The reactions catalyzed by 2OGDs （also called 2ODDs, 2ODQs, and 2OGXs） include but are not limited to demethylation, demethylenation, hydroxytation, halogenation, desaturation, ring cleavage, ring closure, and epimerization （Farrow and Facchini, 2014）.

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Emotional evaluation of lighting in university classrooms:A preliminary study

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Frontiers of Architectural Research 2018年第4期7卷 600-609页

作者： Nuria Castilla Carmen Llinares Fabio Bisegna Vicente Blanca-Gimenez Dpto.Construcciones Arquitectonicas Universitat Politecnica de ValenciaCamino de Veras/nValencia 46022Spain instituto de Investigacion e Innovacion en bioingenieria i3BUniversitat Politecnica de ValenciaCamina de Veras/nValencia 46022Spain dept.of Astronautical Electrical and Energetic Engineering (DIAEE)SAPIENZA University of RomeVia Eudossiana 18Rome 00184Italy

In educational environments an improvement in the quality of interior lighting has a direct benefit in increasing productivity and alertness of students and teachers, as well as very important implications for the energy efficiency of the education facilities but when a replacement with different lighting is analyzed, research may be tarnished by users’ pre-formed opinions, influenced by manufacturers and advertising. Consequently, it is necessary to understand the users’ point of view, even before of being subjected to any change in the lighting *** on the Kansei Engineering framework, the general objective of this paper is to evaluate and compare the subjective evaluation of students’ pre-formed opinions to lighting provided by two types of lamps (fluorescent and LED). The subjective assessment of 427 university students has been compared over four years. The results show significant differences in students’ subjective evaluation. This finding highlights the existence of symbolic or functional attributes of the usefulness perceived by the student that could influence on investigations in which different types of lighting are compared.

关键词： Kansei engineering Lighting Fluorescent LED Subjective assessment University classroom

Tailoring RGD local surface density at the nanoscale toward adult stem cell chondrogenic commitment

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Nano Research 2017年第6期10卷 1959-1971页

作者： Anna Lagunas Iro Tsintzou Yolanda Vida Daniel Collado Ezequiel Perez-Inestrosa Cristina Rodriguez Pereira Joana Magalhaes Jose A. Andrades Josep Samitier Networking Biomedical Research Center (CIBER) C/Monforte de Lemos 3-5 Pabellon 11 Planta 0 Madrid 28029 Spain Institute for Bioengineering of Catalonia (IBEC) Baldiri-Reixac 10-12 Barcelona 08028 Spain instituto de Investigacion Biomedica de Malaga (IBIMA) Department of Organic Chemistry Universidad de Malaga (UMA) Malaga 29071 Spain Andalusian Centre for Nanomedicine and Biotechnology-BlONAND Parque Tecnoldgico de Andalucia Mdlaga 29590 Spain Unidad de bioingenieria Tisulary Terapia Celular (GBTTC-CHUAC) Grupo de Reumatologia Instituto de Investigacion Biomedica de A Coru6a (INIBIC) Complexo Hospitalario Universitario de A Coruf~a (CHUAC) Sergas Universidade da Coruna (UDC) As Xubias 84 Coruna 15006 A Spain Cell Biology Genetics and Physiology Department University de Malaga (UMA) Campus Teatinos Malaga 29071 Spain department of Engineering Electronics University of Barcelona (UB) Marti i Franques 1-11 Barcelona 08028 Spain

Arginine-glycine-aspartic acid （RGD） dendrimer-based nanopatterns on poly（L- lactic acid） were used as bioactive substrates to evaluate the impact of the RGD local surface density on the chondrogenic induction of adult human mesenchymal stem cells. During chondrogenic commitment, active extracellular matrix （ECM） remodeling takes place, playing an instructive role in the differentiation process. Although three-dimensional environments such as pellet or micromass cultures are commonly used for in vitro chondrogenic differentiation, these cultures are rather limited with respect to their ability to interrogate cells in celI-ECM interactions. In the present study, the nanopatterns of the tunable RGD surface density were obtained as a function of the initial dendrimer concentration. The local RGD surface density was quantified through probability contour plots for the minimum interparticle distance, constructed from the corresponding atomic force microscopy images, and correlated with the cell adhesion and differentiation response. The results revealed that the local RGD surface density at the nanoscale acts as a regulator of chondrogenic commitment, and that intermediate adhesiveness of cells to the substrates favors mesenchymal cell condensation and early chondrogenic differentiation.

关键词： dendrimer arginine-glycine-asparticacid (RGD) nanopattem human mesenchymalstem cells (hMSCs) chondrogenesis

Endosomal escape of protein nanoparticles engineered through humanized histidine-rich peptides

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Science China Materials 2020年第4期63卷 644-653页

作者： Hector Lopez-Laguna Rafael Cubarsi Ugutz Unzueta Ramon Mangues Esther Vazquez Antonio Villaverde Institut de Biotecnologia i de Biomedicina Universitat Aut6noma de BarcelonaBellaterra08193 BarcelonaSpain departament de Genetica i de Microbiologia Universitat Aut6noma de BarcelonaBellaterra08193 BarcelonaSpain CIBER de bioingenieria Biomateriales y Nanomedicina(CIBER-BBN)C/Monforte de Lemos 3-528029 MadridSpain departament de Matematiques Universitat Politecnica de Catalunya08034BarcelonaSpain Institut d'Investigacions Biomediques Sant Pau Hospital de la Santa Creu i Sant Pau08025 BarcelonaSpain

Poly-histidine peptides such as H6(HHHHHH)are used in protein biotechnologies as purification tags,protein-assembling agents and endosomal-escape *** pleiotropic properties of such peptides make them appealing to design protein-based smart materials or nanoparticles for imaging or drug delivery to be produced in form of recombinant ***,the clinical applicability of H6-tagged proteins is restricted by the potential immunogenicity of these *** this study,we have explored several humanized histidine-rich peptides in tumor-targeted modular proteins,which can specifically bind and be internalized by the target cells through the tumoral marker *** were particularly interested in exploring how protein purification,self-assembling and endosomal escape perform in proteins containing the variant histidine-rich *** the tested candidates,the peptide H5 E(HEHEHEHEH)is promising as a good promoter of endosomal escape of the associated fulllength protein upon endosomal *** numerical modelling of cell penetration and endosomal escape of the tested proteins has revealed a negative relationship between the amount of protein internalized into target cells and the efficiency of cytoplasmic *** fact demonstrates that the His-mediated,proton sponge-based endosomal escape saturates at moderate amounts of internalized protein,a fact that might be critical for the design of protein materials for cytosolic molecular delivery.

关键词： protein materials nanoparticles genetic design endosomal escape poly-histidines

The Effect of Brain Gym on Global Cognitive Function of Institutionalized Older People

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International Journal of Mental Health Promotion 2022年第3期24卷 375-383页

作者： Araceli Cano-Estrada Araceli Jimenez-Mendoza denny Contreras-Guerrero Mario Hernandes-Alejandro Andres Salas-Casas Escuela Superior de Tlahuelilpan Universidad Autónoma del Estado de HidalgoTlahuelilpanHidalgoMéxico Escuela Nacional de Enfermería y Obstetricia.Universidad Nacional Autónoma de México Mexico CityMéxico departomento de bioingenieria Unidad Profesional Interdisciplinaria de Biotecnología del Instituto Politecnico Nacional(UPIBIIPN)Mexico CityMexico instituto de Ciencias de la Salud.Universidad Autónoma del Estado de Hidalgo.Pachuca Hidalgo.México.Scuela Superior de TlahuelilpanUniversidad Autónoma del Estado de HidalgoTlahuelilpanHidalgoMéxico

Cognitive function of older adults is influenced by several factors such as age,sex,education,and physical activity so that it can impact the decrease in progressive cognitive *** is necessary to implement cognitive training in old people’s care to prevent or delay the onset of age-related cognitive *** gym is a series of simple motion exercises and is an alternative therapy that stimulates the *** study aims to evaluate the effect of brain gym as a cognitive training intervention on global cognitive functioning of institutionalized older *** study is a non-randomized quasi-experimental one using pre-and post-test control group *** participants of the study were 65 years old or older who had been part of gerontological centers from a local *** were divided into two groups:control and intervention,each one with 15 ***-Mental State Examination was applied to the sample before and after the cognitive *** brain gym training consists of bi-weekly group meeting for 12 weeks,lasting 50 min *** treated group had a significantly better global cognitive ***(memory),orientation and language skills were the domains with the most significant changes(pder people lease type your abstract *** of a research paper is typically 200 to 400 words in length,and 150 to 300 words for a review *** shall be running continuously(not structured)and shall not include reference *** that appear only once in the abstract should be defined in *** abbreviations appear more than once,the full definitions should be provided first before they can be used elsewhere.

关键词： Cognitive function brain gymnastic older people gerontological center physical activity

Optimization of Zeaxanthin Production by Immobilized <i>Flavobacterium</i>sp. Cells in Fluidized Bed Bioreactor

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Advances in Microbiology 2012年第4期2卷 598-604页

作者： Ma del Carmen Chavez-Parga Alejandro Munguia-Franco Mayanin Aguilar-Torres Eleazar M. Escamilla-Silva Division de Estudios de Posgrado Facultad de Ingeniería Química Universidad Michoacana de San Nicolas de Hidalgo Morelia Mexico Laboratorio de Biotecnologia y bioingenieria Departamento de Ingenieria Quimica Instituto Tecnologico de Celaya Celaya Mexico

From time immemorial, human beings have used pigments made from vegetables, fruits, superior plants, animal tissues and cereals. One of the greatest sources of pigments is the bacterium that, with the use of the modern technology, has increased the production of metabolites of interest. The microbiological production of carotenoids has not been optimized to obtain pigment production quantities of pigments and carotenoids recovery that lower production costs. The aim of this work was to design a Zeaxanthin production process with Flavobacterium sp. immobilized cells in a fluidized bed bioreactor. An optimum culture medium for Zeaxanthin production in stirred flasks (2.46 g·L–1) was obtained. Furthermore, optimum process conditions for a maximum yield of Zeaxanthin production, by fluidized bed bioreactor, were established. A statistical analysis showed that the most significant factors were air flow, pH and NaCl concentration (4.5 g·L–1). In this study a maximum Zeaxanthin production of 3.8 g·L–1 was reached. The highest reported yield to date was 0.329 g·L–1.

关键词： Fluidized Bed Bioreactor Orthogonal design Flavobacterium sp. Zeaxanthin

Trehalose and Sucrose Osmolytes Accumulated by Algae as Potential Raw Material for Bioethanol

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Natural Resources 2011年第3期2卷 173-179页

作者： Ma.del Pilar Bremauntz Luis G.Torres-Bustillos Rosa-Olivia Canizares-Villanueva Enrique Duran-Paramo Luis Fernández-Linares Laboratory of Bioprocesses Department of BioprocessesUnidad Profesional Interdisciplinaria de Biotecnologia UPIBI-Instituto Politecnico NacionalMexico CityMexico departamento de Biotecnologia y bioingenieria Centro de Investigacion y de Estudios Avanzados-IPNMexico CityMexico

Currently, obtaining sustainable fuels, such as biodiesel and bioethanol, from cheap and renewable materials is a challenge. In recent years, a new approach being developed consists of producing, sugars from algae by photosynthesis. Sugar accumulation can be increased under osmotic stress (osmoregulation). The aim of this study is to show the pro-duction of sugars from algae, isolated from natural sources, and the effect of osmotic stress on fermentable sugars ac-cumulation. Strain isolation, production of sugars from each alga and the effect of osmotic stress on growth and sugar production are described. Twelve algal strains were isolated, showing growths between 0.6 and 1.8 g of biomass dry weight /L, all with production of intracellular and extracellular sugars. The strain identified as Chlorella sp. showed an increase in sugar production from 23.64 to 421 mg of sugars/g of biomass dry weight after 24 h of osmotic stress with 0.4 M NaCl. Sucrose and trehalose, both fermentable sugars, were the compatible osmolytes accumulated in response to the osmotic stress. The isolated strains are potential producers of fermentable sugars, using the photosynthetic pathway and osmotic stress.

关键词： Algae Biofuels Osmoregulators Carbohydrates Bioethanol

Osmotic Stress Effect over Carbohydrate Production in a Native Starin of Scenedesmus sp.

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Natural Resources 2014年第1期5卷 5-9页

作者： Pilar Bremauntz Luis C.Fernandez-Linares Rosa O.Canizares-Villanueva Laboratory of Bioprocesses Department of BioprocessesUnidad Profesional Interdisciplinaria de Biotecnologia(UPIBI)Instituto Politecnico NacionalMexicoD.F.Mexico departamento de Biotecnologia y bioingenieria Centro de Investigacion y de Estudios AvanzadosInstituto Politecnico NacionalMexicoD.F.Mexico

The production of biofuels is currently presented as a possible answer in the search for sustainable alternatives for the total or partial substitution of fossil fuels. One of the most successful biofuels that have been developed is bioethanol. However, bioethanol production has been limited since it relies on the use of sugar cane or cereals. These materials are important sources of food and their demand as both a biofuel and a foodstuff has led to the price increase and may lead to possible shortages. Our group has focused on searching for native microalgae as sources of carbohydrates and bioethanol, with the goal of finding a sustainable source of bioethanol. Currently, twelve different strains which reach growth rates between 0.7 - 1.8 g/L and present carbohydrate production under osmotic shock conditions have been isolated. In this work, we demonstrate the results obtained with the Chlorella sp. [1] strain and the results obtained with the Scenedesmus sp. strain. The Scenedesmus sp. strain showed an increase in the production from 22 to 650 mg/sugar/g of biomass (dry weight), after 24 hours of osmotic shock with 0.1 M NaCl. The osmolytes which were produced after osmotic shock were identified as sucrose and trehalose, both of which are fermentable. These results demonstrate that this strain, through the photosynthetic pathway and osmotic shock, is a potential source of fermentable sugars.

关键词： Biofuels Bioethanol Microalgae Scenedesmus sp. Carbohydrates

Protective Effect of Phenolic-Rich Extracts from Different Parts of <i>Opuntia joconostle</i>Fruit against Carbon Tetrachloride-Induced Oxidative Stress in Mice

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Journal of Biomaterials and Nanobiotechnology 2013年第3期4卷 35-42页

作者： Obed Osorio-Esquivel Alicia Ortiz-Moreno Julieta Herrera-Martínez María Dolores Hernández-Navarro departamento de bioingenieria y Biotecnología Escuela Nacional de Ciencias Biológicas Instituto Politécnico Nacional México City México departamento de Farmacia Facultad de Química Universidad Autónoma del Estado de México Toluca México departamento de Morfología Escuela Nacional de Ciencias Biológicas Instituto Politécnico Nacional México City México

Opuntia joconostle fruit is a rich source of biocompounds such as polyphenols including gallic, vanilic, 4-hidroxybenzoic, cafeic, and syringic acids, catechin, epicatechin, rutin, and vanillin, besides betalains. The objective of this research was to evaluate the effect of supplementation polyphenols-rich extracts from different parts of Opuntia joconostle against carbon tetrachloride-induced oxidative stress in a mouse model. The animals were treated orally with polyphenols-rich extracts at 50, 100 and 200 mg/kg BW for 30 consecutive days. On day 30th the mice received carbon tetrachloride (CCl4) as hepatoxic agent. Biochemical evaluations were carried out 24 h after induction of the oxidative stress. Data showed that methanolic extracts from different parts of Opuntia joconostle exerting protective effect against the CCl4-induced oxidative stress in mice. Histology examination revealed that the damage decreased in groups treated with polyphenols-rich extracts compared to the group that did not receive any treatment. Opuntia joconostle fruit contains many phenolic compounds, flavonoids and betalains. The protective effect of extracts may be related to the phenolic composition and also by a counteraction with other compounds, such as betalains and flavonoids that increase their antioxidant effect.

关键词： Opuntia joconostle Oxidative Stress Carbon Tetrachloride Phenolic Compounds