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Anti-depression effect of Kai Xin San and research of its compatibility

Chinese Medical Journal 2005年第14期118卷 1214-1216页

作者： CHENYi-hong WANGDong-xiao LIUPing WANGJin-liang DAIChang CHENGGui-fang instituteofmatefiamedica ChineseAcademyofMedicalSciencesandPekingUnionMedicalCollegeBeijing100050China ResearchInstituteforClinicalPharmacology. GeneralHospitalofPLABeijing100853China

Kai Xin San (KXS) is a category of traditional complex prescription that wasfirst recorded in Bei Ji Qian Jin Yao Fang by SUN Si-miao in the Tang Dynasty and then also recordedin Tai Ping Hui Min He Ji Ju Fang, Qian Jin Yao Fang and Gu Jin Lu Yan in later generations. It wasmainly used by herbalist doctors to treat symptoms including desolation, moodiness, forgetfulnessetc. , which are similar to the neurosis such as depression, anxiety and difficulty in studying andmemory in the West medicine. The former recorded formulas were all composed of ginseng, polygala,hoelen and acori graminei with different proportion. But there are no systematic studies on theclinical adaptive symptom corresponding to different matching and the best anti-depressive matchingso far. In this study, the classical anti-depressive animal model, namely, ' force swimming model'and 'suspend tail model' were adopted to appraise the anti-depressive effect of the aforementionedformulas and the pharmacodynamical distinction between the whole formula decoction and the activecombination made up with each effective component from ginseng, polygala, hoelen and acori graminewere also investigated, which would provide scientific evidence for properly using KXS in clinic.

关键词： kai xin san anti-depression force swimming suspend tail

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Astilbic Acid Induced COLO 205 cell Apoptosis by Regulating Bcl-2 and Bax Expression and Activating Caspase-3

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浙江省医学科学院学报 2005年第2期16卷 16-21页

作者： ZhengXiao-liang SunHong-xiang LiuXue-li ChenYun-xiang QianBo-chu instituteofmatefiamedica ZhejiangAcademyofMedicalSciencesHangzhou310013 CollegeofAnimalSciences ZhejiangUniversity StateKeyLaboratoryforNewDrugSatetyEvaluation，Zhejiang

To investigate the effect of astilbic acid (3β,6β-dihydroxyolean-12-en-27-oic acid, AA) on human colorectal carcinoma COLO 205 cell proliferation and *** Proliferation of COLO 205 cells was measued by MTT assay. Content of DNA in COLO 205 cell was measued by modified diphenylamine assay. AA-induced morphological changes was observed with fluorescence microscope and transmission electron *** fragmentation was visualized by agarose gel *** rate and cell cycle distribution were deter-mined by flow cytometric *** of Bcl-2 and Bax proteins were visioned by immunohistochemical *** change of relative mitochondral transmembrane potential (MTP) in COLO 205 cell was analyzed with FCM after rhodamine 123 staining. Results The IC50 (96h) of AA for inhibiting COLO 205 cell proliferation was 61.56±0.34 μmol/*** induced a marked concentration- and time-dependent inhibition of COLO 205 cell proliferation and reduced the DNA content in COLO 205 cell. Cells treated with AA 64 μmol/L showed typical morphological changes of apoptosis and DNA “ladder” pattern. The cell cycle was arrested in G0/G1 phase, and the apoptosis rate was 28.25% for COLO 205 cells treated with AA 64 μmol/L for 48h. Meanwhile the expression of Bcl-2 protein was decreased while that of Bax was increased and relative MTP was decreased as well. DEVD-CHO 1μmol/L could increase the viability of COLO 205 cells treated with AA for *** AA showed potent inhibitory activity on COLO 205 cells proliferation,and could induce COLO 205 cells apoptosis through disturbing DNA replication, down-regulating Bcl-2 expression, and up-regulating Bax expression, lowering relative MTP, and activating caspase-3 pathway.

关键词： COLO 205细胞酸化诱导细胞凋亡 Bcl-2 基因表达

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