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Studies of Liposomal bcl-2 Antisense Oligode-oxynucleofide Induction of Apoptosis in Raji Cells

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Chinese Journal of Clinical Oncology 2004年第6期1卷 427-430页

作者： DongmeiHe HuanZhong instituteofhematology JinanUniversityMedicalCollegeGuangzhou510632China.

OBJECTIVE To explore the effect of liposomal G3139 and transfected antisense phosphorothioate oligodeoxynucleotides directed against the coding region of the bcl-2 messenger RNA and the translation site on apoptosis in Raji *** Cytotoxic effects were measured by use of the MTT method; The expression levels of Bcl-2 protein were assayed by immunofiuorescence using a fluoresce isothiocyanate label. Apoptosis was determined by morphological observation and flow cytometric *** The 2 antisense oligonucleotides and G3139 can reduce Bcl-2 protein levels and Raji cell viability (IC50=4.54, 4.72 and 4.26 μmol/L, respectively), and induce apoptosis. A scrambled sequence control oligonucleotide and empty liposomes did not alter cell viability, Bcl-2 protein expression or apoptosis rates. There was no difference in reducing Bcl-2 protein levels and apoptosis rates found among the 3 antisense *** The 2 antisense oligodeoxynucleotides of bcl-2 messenger RNA can effectively induce apoptosis of Raji cells. The 2 antisense sequences and G3139 have a similarity in their antisense effect.

关键词： bcl-2 抗过敏细胞氧化核酸感应现象细胞凋亡 Raji细胞 RNA 肿瘤

Extracorporeal regulation effect of cytokines combination on stem cell or progenitor of umbilical blood

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中国临床康复 2003年第6期7卷 1016-1017页

作者：郭荣何飞孙明陈波斌林果为周宏研 instituteofhematology theUnionHospitalAffiliatedtoTongjiMedicalCollegeHuazhongUniversityofScienceandTechnology DepartmentofCardiovascular AffiliatedXiangyaHospitalofXiangyaMedicalCollegeofMiddleSouthUniversityChangsha DepartmentofHematology HuashanHospitalShanghaiMedicalUniversity

AIM:To study the regulation effect of different cytokines combinations on stem cell or progenitor of umbilical blood when no blood serum and matrix exist. METHODS: Collect and analyze the 23 sample of umbilical blood. RESULTS:(1) The combination of SCF+Flk2/Flt3 ligand and(FL)±TPO can amplify CD34,+,CD34+Thy 1+,CD34+CD33+and LTC IC in umbilical blood effectively and rapidly.(2) After add solubility type (sIL 6R)of IL 6 and IL 6 receptor into this combination, the stem cell or progenitor proliferated prominently;When add IL 6 alone(without sIL 6R), the content of CD34+cells and LTC IC didn’t increased obviously in early stage, but the amplification of CFU Mix and BFU E was not prominent as CFU GM. (3)Detect apoptosis rate of cells by FITC Annexin Ⅴlabeled by membranous change in early stage of cellular apoptosis. After add Flt3L and /or IL 6+sIL 6R, the Annexin Ⅴpositive cells decreased from 15.2%～19.1%to 2.8%～3.5%. CONCLUSION: In suspending system composed with SCF+TPO+Flt3L+IL 6+sIL 6R without matrix and blood serum, the umbilical blood can not only produce large amount of committed progenitor but also keep certain quantitive hematopoietic cells of early stage.

关键词：细胞因子组合脐血干/祖细胞调控作用

ANTI-HUMAN PLATELET TETRASPANIN(CD9)MONOCLONAL ANTIBODIES INDUCE PLATELET INTEGRIN αbβ3 ACTIVATION IN A Fc RECEPTOR INDEPENDENT FASHION

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Chinese Medical Sciences Journal 2000年第3期15卷 145-149页

作者：武怀珠李家增彭林刘汉芝武文杰周玉玲侯庆明孔德洪 instituteofhematology CAMS＆PUMCTianjin300020 QufuHospital QufuShandong273100

This study characterized the activation of platelet integrin α bβ3 induced by two anti human platelet tetraspanin monoclonal antibodies(mAbs),HI117 and SJ9A4. *** 125 I labeled human fibrinogen(Fg),specific Fg binding to human platelets induced by HI117 and SJ9A4 was measured as indication of activation of platelet integrin αbβ3 by the two mAbs. ***117 and SJ9A4(10μg/ml and 20μg/ml) induced evident specific Fg binding to human platelets,suggesting that the two mAbs evoked activation of platelet integrin αbβ*** study indicated that HI117 and SJ9A4 induced integrin αⅡbβ3 activation independent of platelet Fc receptors, and that HI117 and SJ9A4 induced integrin αbβ3 activation was inhibited by sphingosing, aspirin, apyrase, and/or PGI2. *** anti platelet tetraspanin(CD9)mAbs,HI117 and SJ9A4, can induce platelet integrin αⅡbβ3 activation independent of Fc *** signaling pathways,***,secreted ADP, and cAMP pathways may be involved in the process,with protein kinase C activation presumably being the common step of the three pathways.

关键词： bβ3 活化单克隆抗体血小板纤维蛋白原蛋白激酶C

Clinical Analysis of 43 Patients with Ught Chain Multiple Myeloma

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Chinese Journal of Clinical Oncology 2004年第3期1卷 215-220页

作者： XiuliCong XiujuanSun LinshengQian ZhongchaoHan instituteofhematology＆HospitalofBloodDiseases ChineseAcademyofMedicalSciences＆PekingUnionofMedicalCollegeTianjin300020China.

OBJECTIVE To investigate the clinical characteristics and laboratory findings of patients with light-chain multiple myeloma. METHODS Fourty-three patients with light chain myeloma over a 13year period were analyzed retrospectively and 43 cases with IgG type myeloma in the same period were used as control. RESULTS Of the 43 patients, 28 were male, 15 were female, with an overall mean age of 57 years (range, 36-71). At the time of onset, the main symptoms were fatigue and dizziness (23 cases, 53.5%) and bone pain (25, 58.1%). The main signs were anemia (28, 65.1%) and bone pressure pain (23, 53.5%). Of 39 patients with determined staging, 38 were in stage Ⅲ and 1 stage Ⅰ. Renal function examinations were performed for 31 patients. Among them, 16 were in stage ⅢB and 15 in ⅡA. Hypercalcemia (≥3 mmol/L) occurred in 2 cases. Of 18 patients, 3 had proteinuria ≥12 g per 24 hours. Osteolytic lesions appeared in 27 of 31 cases. No abnormal globulin peaks were found in the serum protein electrophoretic bands. Serum and urine immunoelectrophoresis showed that 10 cases were kappa light chain, 29 were lambda light chain and 4 were both. Nineteen patients received chemotherapy, of which 8 cases obtained complete remission and 11 had no remission. CONCLUSION Because of poor differentiation, skeletal destruction and renal dysfunction, light chain multiple myeloma patients have meager therapeutical efficacy and poor prognosis.

关键词：临床作用多样骨髓癌肿瘤 IgG 变态反应

Interferon a： the salvage therapy for patients with unsatisfactory response to minimal residual disease-directed modified donor lymphocyte infusion

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Chinese Medical Journal 2014年第14期 2583-2587页

作者： MoXiaodong ZhaoXiangyu XuLanping LiuDaihong ZhangXiaohui ChenHuan WangYu HuangXiaojun BeijinnKeyLaboratoryofHematopoieticStemCellTransplantation InstituteofHematologyPeople'sHospitalPekingUniversityBeiiing100044China

Background Minimal residual disease （MRD）-directedmodified donor lymphocyte infusion （mDLI） is used to treat relapse after hematopoietic stem cell transplantation （HSCT）. For patients who experience an unsatisfactory response tomDLI relapse is usually inevitable. Therefore we sought to evaluate the efficacy ofinterferon a therapy in these patients. Methods Regular MRD monitoring was carried out after the HSCT. The patients who were MRD-positive underwent mDLI. Patients with an unsatisfactory response to mDLI received interferon a therapy （3 million units, twice weekly） with regular monitoring of MRD. To ensure the immunomodulatory effects of interferon a, immunosuppressant treatment would be stopped before interferon a treatment. Results Five patients with an unsatisfactory response to mDLI treatment received interferon a （3 had t（8;21） chromosomal translocation acute myeloid leukemia, and 2 had common acute leukemia）. They had significantly reduced or resolved MRD. Four patients developed chronic graft-versus-host disease. Two of the 5 patients reported transient fevers, and no significant bone marrow suppression was observed. All of them were in continuous complete remission after interferon a treatment. The median survival time was 469 days （range 368-948 days）. Conclusions In patients with an unsatisfactory response to MRD-directed mDLI, interferon a may directly or indirectly induce the graft-versus-leukemia effect to improve mDLI efficacy and clear MRD. Chin Med J 2014;127 （14）： 2583-2587

关键词： interferon a hematopoietic stem cell transplantation donor lymphocyte infusion

THE INHIBITORY EFFECT OF DEFEROXAMINE ON DNA SYNTHESIS IN HUMAN LYMPHOCYTES

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中华医学杂志(英文版) 1989年第12期102卷 905-905页

作者： Wang Shu-tong Jin Kun-lin Li Tian-hong instituteofhematology People'sHospital BeijingMedicalUniversity Beijing

As an iron-chelating agent, deferoxamine (DFO) is widely used in treating iron poisoning and disorders of iron overload. This study demonstrates that DFO is a potent S-phase inhibitor of DNA synthesis in human lymphocytes in vitro, and this inhibitory effect of DFO is reversible by adding appropnate amounts of ferric ion. As a nontoxic and selective-S-phase inhibitor, it may play a role in immunosuppression in experimental and therapeutic situations. It may even become an auxiliary therapy for leukemia or other malignant tumors.

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Clonal Expansion and Cytotoxicity of TCRVβ Subfamily T Cells Induced by CML and K562 Cells

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Chinese Journal of Clinical Oncology 2004年第1期1卷 47-52页

作者： YupingZHang YangqiuLi ShaohuaChen LijianYang GengxinLuo XueliZhang DepartmentofHematology FirstAffiliatedMunicipalHospitalGuangzhou510180China. instituteofhematology MedicalCollegeJinanUniversityGuangzhou510632China.

OBJECTIVE To investigate the anti-leukemia effect, the distribution and clonal expansion of TCRVβ subfamily T cells in T cells from cord blood and adult peripheral blood induced by CML cells and K562 cells in vitro. METHODS Peripheral blood T cells from one adult donor and 3 cases of cord blood were stimulated with CML cells and K562 cells and further amplified by a suspended T cell-bulk culture,in order to induce CML specific cytotoxic T lymphocytes. The induced T cells were further analyzed for the specific cytotoxicity in CML by LDH assay, the phenotype identification by indirect immunofiuorescence technique and the distribution and clonal expansion of TCRVβ subfamily by using reverse transcriptase-polymerase chain reaction (RT-PCR) and genescan analysis, respectively. RESULTS Oligoclonal and oligoclonal tendency T cells with higher specific cytotoxicity from cord blood and adult peripheral blood could be induced by stimulation with CML cells and K562 cells. CONCLUSIONS Specific cytotoxic T cells for an anti-CML effect could be induced by CML cells and K562 cells .The induced T cells which have the characteristic of specific cytotoxicity against CML cells may come from the clonal expansion of TCRVβ subfamily T cells.

关键词： TCRVβ T淋细胞亚群白血病治疗血液

Amplification of Surface Antigen P43 Gene and Its Application in Detection of Toxoplasma Gondii in Allogeneic Hematopoietic Stem Cell Transplantation

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The Chinese-German Journal of Clinical Oncology 2002年第4期1卷 238-240页

作者： ZHOUYongan YUXinbing instituteofhematology TheSecondAffiliatedHospitalofShanxiMedicalU－niversityTaiyuan030001China DepartmentofParasitology MedicalCollegeSunYat－senUniversityGuan－gzhou510089China

Objective:To establish a rapid,specific and sensitive diagnostic technique for the human Toxoplasma gondii infection in the recipi-ents with allogeneic hematopoietic stem cell transplantation and discuss its clinical ***:30 patients undergoing allogeneic hematopoietic stem cell transplantation were detected by using ELISA and ***:Among 30 recipients undergiong allogeneic hematopoietic stem cell transplantation,3 were positive for Toxoplasma gondiii antigen and 5 for surface antigen p43 gene with the positive rate being 13.3% and 16.67% respectively.20 healthy people(negative for anti-Tox antibody)were also tested by using ELISA and ***:PCR is an accurate,relatively rapid,sensitive and specific method for detecting P43 gene of Toxoplasma ***-canuse PCR can be applied to a variety of different clinical samples,it can be considered as a valuable additional tool for identification of Toxoplasma gondii infections.

关键词：同种异基因造血干细胞移植鼠弓形体检测表面抗原 P43 基因扩增

Inhibition of mouse hepatocyte apoptosis via anti-Fas ribozyme

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World Journal of Gastroenterology 2004年第17期10卷 2567-2570页

作者： MinZhang WeiHe FangLiu PingZou JuanXiao Zhao-DongZhong Zhong-BoHu instituteofhematology UnionHospitalTongjiMedicalCollegeHuazhongUniversityofScienceandTechnologyWuhan430022HubeiProvinceChina

AIM: TO investigate the effects of anti-Fas ribozyme on Fas expression and apoptosis in primary cultured mouse ***: Mouse hepatocytes were isolated by using collagenase irrigation. A hammerhead ribozyme targeting the Fas mRNA was constructed, and transfected into mouse hepatocytes via Effectene. Then Fas expression in mouse hepatocytes was detected by RT-PCR and western *** being treated with anti-Fas antibody (JO2), hepatocytes viability was measured with MTI- assay. Caspase-3 proteolytic activity was detected, and cell apoptosis was measured according to Annexin V-FITC apoptosis detection ***: Fas expressed in primary mouse *** expression in hepatocytes transfected with anti-Fas ribozyme was decreased remarkably and correlated with the resistance to Fas-mediated apoptosis as determined by flow cytometry and caspase-3 proteolytic ***: AnU-Fas ribozyme can remarkably decrease the Fas expression in mouse hepatocytes, thus inhibit Fasmediated apoptosis in hepatocytes, It is suggested that anti-Fas ribozyme could significantly increase the resistance of transplanted hepatocytes to apoptosis and improve the survival of transplanted hepatocytes,

关键词：抑制作用老鼠肝实质细胞细胞调亡抗-Fas 核糖酶消化系统