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Journal of Integrative plant Biology 2012年第6期54卷 412-421页

作者： Nai-Qin Zhong Li-Bo Han Xiao-Min Wu Li-Li Wang Fang Wang Yan-He Ma Gui-Xian Xia institute of microbiology Chinese Academy of SciencesBeijing 100101 State Key Laboratory of plant Genomics Beijing 100101

AaNhaD, a gene isolated from the soda lake alkaliphile Alkalimonas amylolytica, encodes a Na＋/H＋ antiporter crucial for the bacterium＇s resistance to salt/alkali stresses. However, it remains unknown whether this type of bacterial gene may be able to increase the tolerance of flowering plants to salt/alkali stresses. To investigate the use of extremophile genetic resources in higher plants, transgenic tobacco BY-2 cells and plants harboring AaNhaDwere generated and their stress tolerance was evaluated. Ectopic expression of AaNhaD enhanced the salt tolerance of the transgenic BY-2 cells in a pH-dependent manner. Compared to wild-type controls, the transgenic cells exhibited increased Na＋ concentrations and pH levels in the vacuoles. Subcellular localization analysis indicated that AaNhaD-GFP fusion proteins were primarily localized in the tonoplasts. Similar to the transgenic BY-2 cells, *** tobacco plants displayed enhanced stress tolerance when grown in saline-alkali soil. These results indicate that AaNhaD functions as a pH-dependent tonoplast Na＋/H＋ antiporter in plant cells, thus presenting a new avenue for the genetic improvement of salinity/alkalinity tolerance.

关键词： Alkaliphiles Alkalimonas amylolytica Na+/H+ antiporter tobacco BY-2 cells high salinity stress alkaline.

The RING Finger Protein Nt RCP1 Is Involved in the Floral Transition in Tobacco(Nicotiana tabacum)

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Journal of Genetics and Genomics 2015年第6期42卷 311-317页

作者： Hai-Yun Wang Yi Yu Yong-Duo Sun Li-Bo Han Xiao-Min Wu Jia-He Wu Gui-Xian Xia Guo-Qin Liu State Key Laboratory of plant Physiology and Biochemistry College of Biological SciencesChina Agricultural University State Key Laboratory of plant Genomics Institute of MicrobiologyChinese Academy of Sciences

The transition from the vegetative phase to the reproductive phase is a major developmental process in flowering *** underlying mechanism controlling this cellular process remains a research focus in the field of plant molecular *** the present work,we identified a gene encoding the C3H2C3-type RING finger protein Nt RCP1 from tobacco BY-2 *** analysis demonstrated that Nt RCP1 is a functional E3 ubiquitin *** tobacco plants,expression level of Nt RCP1 was higher in the reproductive shoot apices than in the vegetative *** RCP1-overexpressing plants underwent a more rapid transition from the vegetative to the reproductive phase and flowered markedly earlier than the wild-type *** analysis revealed that the shoot apical meristem of Nt RCP1-overexpressing plants initiated inflorescence primordia precociously compared to the wild-type plant due to accelerated cell *** of Nt RCP1 in BY-2 suspension cells promoted cell division,which was a consequence of the shortened G2 phase in the cell ***,our data suggest that Nt RCP1 may act as a regulator of the phase transition,possibly through its role in cell cycle regulation,during vegetative/reproductive development in tobacco plant.

关键词： RING finger protein Floral transition Cell division cycle Tobacco

Molecular Characterization of Banana streak virus Isolate from Musa Acuminata in China

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Virologica Sinica 2011年第6期26卷 393-402页

作者： Jun Zhuang Jian-hua Wang Xin Zhang Zhi-xin Liu Agricultural Ministry Key Laboratory of tropical Crop Biotechnology Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences Haikou 571101 China institute of plant Virology Fujian Agriculture and Forestry University Fuzhou 350002 China Environment and plant protection institute Chinese Academy of Tropical Agricultural Sciences Danzhou 571734 China

Banana streak virus （BSV）, a member of genus Badnavirus, is a causal agent of banana streak disease throughout the world. The genetic diversity of BSVs from different regions of banana plantations has previously been investigated, but there are relatively few reports of the genetic characteristic of episomal （non-integrated） BSV genomes isolated from China. Here, the complete genome, a total of 7722bp （GenBank accession number DQ092436）, of an isolate of Banana streak virus （BSV） on cultivar Cavendish （BSAcYNV） in Yunnan, China was determined. The genome organises in the typical manner of badnaviruses. The intergenic region of genomic DNA contains a large stem-loop, which may contribute to the ribosome shift into the following open reading frames （ORFs）. The coding region of BSAcYNV consists of three overlapping ORFs, ORF 1 with a non-AUG start codon and ORF2 encoding two small proteins are individually involved in viral movement and ORF3 encodes a polyprotein. Besides the complete genome, a defective genome lacking the whole RNA leader region and a majority of ORF1 and which encompasses 6525bp was also isolated and sequenced from this BSV DNA reservoir in infected banana plants. Sequence analyses showed that BSAcYNV has closest similarity in terms of genome organization and the coding assignments with an BSV isolate from Vietnam （BSAcVNV）. The corresponding coding regions shared identities of 88% and -95% at nucleotide and amino acid levels, respectively. Phylogenetic analysis also indicated BSAcYNV shared the closest geographical evolutionary relationship to BSAcVNV among sequenced banana streak badnaviruses.

关键词： BSAcYNV Complete genome Defective genome Identity Phylogenetic analysis

A New Degraded Sesquiterpene from Marine Actinomycete Streptomyces sp. 0616208

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Chinese Chemical Letters 2006年第11期17卷 1463-1465页

作者： Xiu Chao XIE Wen Li MEI You Xing ZHAO Kui HONG Hao Fu DAI State Key Laboratory. of tropical Crops Biotechnology Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agriculture Sciences Haikou 571101 State Key Laboratory of Phytochemistry and plant Resources in West China Kunming Institute of Botany Chinese Academy of Sciences Kunming 650204

A new degraded sesquiterpene was isolated from the marine actinomycete Streptomyces sp. 0616208. Its structure was elucidated as （1α; 4aα; 5α, 7β, 8aβ）-5, 8a-dimethyl-decahydrona- phthalene-1, 4a, 7-triol on th... 详细信息

关键词： Marine actinomycete, Streptomyces sp., degraded sesquiterpene, (1α 4aα 5α, 7β, 8aβ)-5, 8a-dimethyl-decahydronaphthalene- 1,4a,7-triol.

Loss-of-function mutation of rice SLAC7 decreases chloroplast stability and induces a photoprotection mechanism in rice

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Journal of Integrative plant Biology 2015年第12期57卷 1063-1077页

作者： Xiaolei Fan Jiemin Wu Taiyu Chen Weiwei Tie Hao Chen Fei Zhou Yongjun Lin National Key Laboratory of Crop Genetic Improvement and National Centre of plant Gene Research Huazhong Agricultural University institute of tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences

plants absorb sunlight to power the photochem- ical reactions of photosynthesis, which can potentially damage the photosynthetic machinery. However, the mech- anism that protects chloroplasts from the damage remains unclear. In this work, we demonstrated that rice （Oryza sativa L.） SLAC7 is a generally expressed membrane protein. Loss- of-function of SLAC7 caused continuous damage to the chloroplasts of mutant leaves under normal light conditions. Ion leakage indicators related to leaf damage such as H^O2 and abscisic acid levels were significantly higher in slac7-1 than in the wild type. Consistently, the photosynthesis efficiency and Fv/Fm ratio of slac7-1 were significantly decreased （similar to photoinhibition）. In response to chloroplast damage, slat7- 1 altered its leaf morphology （curled or fused leaf） by the synergy between plant hormones and transcriptional factors to decrease the absorption of light, suggesting that a photoprotection mechanism for chloroplast damage was activated in slac7-1. When grown in dark conditions, slac7-1 displayed a normal phenotype. 5LAC7 under the control of the AtSLAC1 promoter could partially complement thephenotypes of Arabidopsis slacl mutants, indicating a partial conservation of SLAC protein functions. These results suggest that SLAC7 is essential for maintaining the chloroplast stability in rice.

关键词： Anion transport chloroplast cytokinin Oryza sativa L photoinhibition SLAC1

Agrobacterium tumefaciens-mediated transformation of embryogenic callus and CRISPR/Cas9-mediated genome editing in‘Feizixiao'litchi

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Horticultural plant Journal 2023年第5期9卷 947-957页

作者： Shujun Wang Guo Wang Huanling Li Fang Li Jiabao Wang Environment and plant Protection institute Chinese Academy of Tropical Agricultural SciencesHaikouHainan 571101China National Key Laboratory for tropical Crop Breeding HaikouHainan 571101China

Litchi(Litchi chinensis Sonn.)is a type of commercially prevalent subtropical and tropical *** litchi has a highly heterozygous genetic background and a long reproductive cycle,conventional breeding methods(such as hybridization)have limited ability to nurture new litchi ***,an efficient and stable Agrobacterium tumefaciens-mediated genetic transformation of embryogenic callus was established in‘Feizixiao’*** materials were verified using polymerase chain reaction(PCR)analysis,β-glucuronidase(GUS)assay,and green fluorescent protein(GFP)*** implement the technology of the Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR)/associated protein 9(CRISPR/Cas9)technology in‘Feizixiao’litchi and verify the validity of these transformation systems,the litchi polyphenol oxidase gene(LcPPO,JF926153)was knocked *** categories of mutations,covering base insertions,deletions,and substitutions,were found in transgenic materials via sequence *** transformation system achieved high feasibility and efficiency,and the system of CRISPR/Cas9 was successfully employed to edit genes in‘Feizixiao’*** work provides an essential foundation for investigating the functions of genes and accelerating litchi genetic improvement.

关键词： Litchi Litchi chinensis Sonn Genetic transformation Gene editing Polyphenol oxidase(PPO)

A new cytotoxic cardenolide from the latex of Antiaris toxicaria

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Chinese Chemical Letters 2009年第4期20卷 450-452页

作者： Yu Juan Gan Wen Li Mei You Xing Zhao Hao Fu Dai institute of tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences Haikou 571101 China State Key Laboratory of Phytochemistry and Plant Resources in West China Kunming Institute of Botany Chinese Academy of Sciences Kunming 650204 China

A new cardenolide, toxicarioside E （1）, was isolated from the latex ofAntiaris toxicaria （Pers.） Lesch （Moraceae）. Its structure was elucidated on the basis of spectral data and chemical evidence. Compound 1 showed significant cytotoxicity against K562 and SGC-7901 cell lines in vitro by MTT method with the IC50 value of 0.026 and 0.027 μg/mL, respectively.

关键词： Antiaris toxicaria Moraceae Toxicarioside E Cardenolide Cytotoxicity

A new cytotoxic 2-(2-phenylethyl)chromone from Chinese eaglewood

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Chinese Chemical Letters 2008年第8期19卷 934-936页

作者： Jun Liu Jiao Wu You Xing Zhao Yuan Yuan Deng Wen Li Mei Hao Fu Dai institute of tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences Haikou 571101 China State Key Laboratory of Phytochemistry and plant Resources in West China Kunming Institute of Botany Chinese Academy of Sciences Kunming 650204 China

A new compound 8-chloro-5,6,7-trihydroxy-2-（3-hydroxy-4-methoxyphenethyl）-5,6,7,8-tetralaydro-4H-chromen-4-one （1） was isolated from the Chinese eaglewood [Aquilaria sinensis （Lour.） Gilg]. Its structure was elucidated on the basis of spectral data. Compound I showed cytotoxicity against human gastric cancer cell line （SGC-7901） in vitro by MTT method with the IC50 value of 14.6 μg/mL.

关键词： Chinese eaglewood Aquilaria sinensis Chromone Cytotoxicity

Two new epimeric pairs of iridoid from mangrove plant Scyphiphora hydrophyllacea

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Chinese Chemical Letters 2007年第12期18卷 1509-1511页

作者： Yan Bo Zeng Wen Li Mei You Xing Zhao Ling Zhuang Kui Hong Hao Fu Dai State Key Biotechnology Laboratory of tropical Crops institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences Haikou 571101 China State Key Laboratory of Phytochemistry and plant Resources in West China Kunming institute of Botany Chinese Academy of Sciences Kunming 650204 China

Two new epimeric pairs of iridoid scyphiphin A1 （la）, A2 （lb） and scyphiphin B 1 （2a）, B2 （2b） were isolated from Scyphiphora hydrophyllacea Gaertn. F. Their structures were elucidated by spectroscopic methods. The mixture of scyphiphin B 1 and scyphiphin B2 showed moderate cytotoxicity against human hepatoma SMMC-7721 cell line in vitro by MTT method.

关键词： Scyphiphora hydrophyllacea Gaertn. E Iridoid Cytotoxicity SMMC-7721

Down-regulation of MeMYB2 leads to anthocyanin accumulation and increases chilling tolerance in cassava(Manihot esculenta Crantz)

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The Crop Journal 2023年第4期11卷 1181-1191页

作者： Xin Guo Xiaohui Yu Chenyu Lin Pingjuan Zhao Bin Wang Liangping Zou Shuxia Li Xiaoling Yu Yinhua Chen Peng Zhang Ming Peng Mengbin Ruan College of plant Science&Technology Huazhong Agricultural UniversityWuhan 430070HubeiChina National Key Laboratory for tropical Crop Breeding Sanya 572025HainanChina Key Laboratory of Biology and Genetic Resources of tropical Crops Institute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agricultural SciencesHaikou 571101HainanChina Key Laboratory for Biology and Genetic Resources of tropical Crops of Hainan Province Hainan Institute for Tropical Agricultural ResourcesHaikou 571101HainanChina College of tropical Crops Hainan UniversityHaikou 570228HainanChina f Sanya Research institute Chinese Academy of Tropical Agricultural SciencesSanya 572025HainanChina National Key Laboratory of plant Molecular Genetics Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 200032China

Chilling-induced accumulation of reactive oxygen species(ROS) is harmful to plants,which usually produce anthocyanins to scavenge ROS as protection from chilling *** a tropical crop,cassava is hypersensitive to chilling,but the biochemical basis of this hypersensitivity remains *** previously generated Me MYB2-RNAi transgenic cassava with increased chilling *** we report that Me MYB2-RNAi transgenic cassava accumulated less ROS but more cyanidin-3-O-glucoside than the wild type under early chilling *** this stress,the anthocyanin biosynthesis pathway was more active in Me MYB2-RNAi lines than in the wild type,and several genes involved in the pathway,including Me TT8,were up-regulated by Me MYB2-RNAi in the transgenic *** MYB2 bound to the Me TT8 promoter and blocked its expression under both normal and chilling conditions,thereby inhibiting anthocyanin *** TT8 was shown to bind to the promoter of Dihydroflavonol 4-reductase(Me DFR-2)and increased Me DFR-2 *** MYB2 appears to act as an inhibitor of chilling-induced anthocyanin accumulation during the rapid response of cassava to chilling stress.

关键词： Cassava Early chilling stress Anthocyanins MeMYB2 bHLH transcription factor