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Chinese Science Bulletin 2013年第16期58卷 1919-1930页

作者： MAKSUP Sarunyaporn SUPAIBULWATANA Kanyaratt SELVARAJ Gopalan Department of biotechnology Faculty of Science Mahidol University plant biotechnology institute National Research Council of Canada

Rice (Oryza sativa L.) is important to food security and is also an excellent model plant for numerous cereal crops. A functional genomics study in rice includes characterization of the expression dynamics of genes by quantitative real-time PCR (qPCR) analysis; this is a significant key for developing rice varieties that perform well in the face of adverse climate change. The qPCR analysis requires the use of appropriate reference genes in order to make any quantitative interpretations meaningful. Here, the new potential reference genes were selected from a huge public database of rice microarray experiments. The expression stability of 14 candidates and 4 conventional reference genes was validated by geNorm PLUS and NormFinder software. Seven candidates are superior to the conventionally used reference genes in qPCR and three genes can be used reliably for quantitating the expression of genes involved in abiotic stress responses. These high-quality references EP (LOC_Os05g08980), HNR (LOC_Os01g71770), and TBC (LOC_Os09g34040) worked very well in three indica genotypes and one japonica genotype. One of indica genotypes including the Jasmine rice, KDML105 developed in Thailand for which no reference genes have been reported until now.

关键词：功能基因组学胁迫反应非生物水稻胁迫条件籼稻品质实时定量PCR

Maximizing biomass utilization: An integrated strategy for coproducing multiple chemicals

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Journal of Energy Chemistry 2025年第1期100卷 180-191页

作者： Byeongchan Ahn Sua Jo Jonggeol Na J.Jay Liu Young-Ju Kim Wangyun Won Department of Chemical and Biological Engineering Korea University Department of Chemical Engineering (Integrated process) Kyung Hee University Department of Chemical Engineering and Materials Science Ewha Womans University Department of Chemical Engineering Pukyong National University Resources Engineering plant Research Department Korea Institute of Geoscience and Mineral Resources

Lignocellulosic biomass is one of the viable solutions to alleviate the global warming. However, the limited utilization of biomass majorly focused on cellulose and hemicellulose restricts the economic and environmental feasibilities. To cope with this issue, we proposed an integrated process of co-producing 1,6-hexanediol（1,6-HDO） with tetrahydrofuran and adipic acid from biomass, referred to as Strategy A. To compare the impacts of lignin upgrading and feedstock, Strategy B, which co-produces tetrahydrofuran alone, and Strategy C, which is the traditional route to produce 1,6-HDO from fossil fuels, were used. Heat networks are also designed to reduce operating costs and indirect carbon emissions due to energy consumption, saving 87% and 83% of the heat and cooling requirements, respectively, in Strategy A. The market competitiveness of Strategy A was evaluated by determining the minimum selling price through techno-economic analysis, and sustainability was thoroughly investigated by quantifying the environmental impacts through both midpoint and endpoint life-cycle assessments（LCAs）.Strategy A was found to be the most favorable both economically（USRDSCHARDOLLAR3,402/ton） and environmentally（-26.9 kg CO2eq.）. This indicates that lignin valorization is not only economically but also environmentally preferred. Finally, changes in economic and environmental feasibilities depending on economic, process, and environmental parameters were investigated using sensitivity and uncertainty analyses. The results of these analyses provide valuable insight into bio-based chemical production.

关键词： Biorefinery Carbon mitigation Climate change Global warming Lignin utilization Bioeconomy

Big Roles of Small Kinases:The Complex Functions of Receptor-Like Cytoplasmic Kinases in plant Immunity and Development

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Journal of Integrative plant Biology 2013年第12期55卷 1188-1197页

作者： Wenwei Lin Xiyu Ma Libo Shan Ping He Department of plant Pathology and Microbiology and Institute for Plant Genomics and BiotechnologyTexas A&M UniversityCollege Station Department of Biochemistry and Biophysics and Institute for Plant Genomics and BiotechnologyTexas A&M UniversityCollege Station

plants have evolved a large number of receptor-like cytoplasmic kinases （RLCKs） that often functionally and physically associate with receptor-like kinases （RLKs） to modulate plant growth, development and immune responses. Without any apparent extracellular domain, RLCKs relay intracellular signaling often via RLK complex-mediated transphosphorylation events. Recent advances have suggested essential roles of diverse RLCKs in concert with RLKs in regulating various cellular and physiological responses. We summarize here the complex roles of RLCKs in mediating plant immune responses and growth regulation, and discuss specific and overlapping functions of RLCKs in transducing diverse signaling pathways.

关键词： Phosphorylation plant development plant immunity receptor-like cytoplasmic kinases.

Recombination with coat protein transgene in a complementation system based on Cucumber mosaic virus (CMV)

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Science China(Life Sciences) 2001年第3期44卷 263-273页

作者：雷万里方荣祥张国华陈晓英张晓勤 Laboratory of plant biotechnology Institute of Microbiology Chinese Academy of Sciences Beijing China

In order to study the feasibility of Cucumber mosaic virus (CMV) as an expression vector, the full-length cDNA of RNA 3 from strain SD was cloned and the sequence around the start codon of the coat protein (CP) gene was modified to create an Nsi I site for insertion of foreign genes. The CP gene was replaced by the green fluorescent protein (GFP) gene. The cDNAs of Fny RNAs 1 and 2 and the chimeric SD RNA 3 were cloned between the modified 35S promoter and terminator. Tobacco protoplasts were transfected with a mixture of the viral cDNAs containing 35S promoter and terminator as a replacement vector and expressed GFP. A complementation system was established when the replacement vector was inoculated onto the transgenic tobacco plants expressing SD-CMV CP. GFP was detected in the inoculated leaves in 5 of 18 tested plants and in the first upper systemic leaf of one of the 5 plants ten days after inoculation. However, no GFP could be detected in all the plants one month after inoculation. Recombination between the CMV vector and the CP transgene was proved by retro-transcriptional polymerase chain reaction (RT-PCR) and verified by DNA sequencing. Our results argue against the feasibility of the CMV-based replacement vector trans-complemented by the CP transgene, and at the same time, enlighten ways to improve the CMV-based expression vector and the biosafety of CMV CP-mediated virus resistant transgenic plants.

关键词： cucumber mosaic virus complementation vector CP transgene recombination biosafety.

Characterization of an ethylene receptor homolog gene from rice

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Science China(Life Sciences) 2003年第4期46卷 370-378页

作者：曹宛虹董亿张劲松陈受宜 plant biotechnology Lab Institute of Genetics and Developmental BiologyChinese Academy of Sciences

Ethylene plays important roles in plant growth, development and stress responses. Its receptor genes have been studied in dicots such as Arabidopsis, tobacco and tomato. However, no research has been reported for the ethylene receptors from monocots currently. In the present study, we cloned an ethylene receptor gene OSPK2 from rice and found that its encoded protein was divergent from the ethylene receptors from dicots. OSPK2 had a long extension in its N- terminal, followed by three transmembrane segments, a GAF domain, a putative kinase domain and a putative receiver domain. Although most of the domains were conserved, the expected phosphorylation site His and the phosphate receiver Asp have been replaced by Gln and Asn, respectively. This fact indicates that OSPK2 may not function as a histidine kinase in a phosphorelay manner, but rather play roles by other mechanism, probably through Ser/Thr kinase activity. The expression of the OSPK2 gene was investigated by RT-PCR method under different conditions. We found that this gene was apparently induced by wounding and PEG treatment, but not significantly affected by salt and ABA treatments. The differential expression of the OSPK2 gene may reflect its roles in mediating different abiotic stress responses, consistent with our previous studies on tobacco ethylene receptors.

关键词： ethylene receptor, rice, stress, expression.

Fine-mapping of an Arabidopsis cell death mutation locus

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Science China(Life Sciences) 2000年第2期43卷 138-145页

作者：牟中林戴亚李家洋 plant biotechnology Laboratory Institute of Genetics Chinese Academy of Sciences Beijing China

An Arabidopsis cell death mutation locus was mapped to chromosome 2 between lGS1 and mi421. The YAC clone ends, CIC9A3R, CIC11C7L, CIC2G5R and RFLP marker CDs3 within this interval, were used to probe TAMU BAC library and 31 BAC clones were obtained. A BAC contig encompassing the mutation locus, which consists of T6P5, T7M23, T12A21, T8L6 and T18A18, was identified by Southern hybridization with the BAC ends as probes. 11 CAPS and 12 STS markers were developed in this region. These results will facilitate map-based cloning of the genes and sequencing of the genomic DNA in this region.

关键词： Arabidopsis cell death mod1 mutant physical map CAPS marker.

Isolation and characterization of a new defense gene from soybean

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Science China(Life Sciences) 2001年第4期44卷 409-420页

作者：贺超英吴晓雷东方阳杜保兴张劲松陈受宜 plant biotechnology Laboratory Institute of Genetics Chinese Academy of Sciences Beijing China

A cDNA clone of a single-copy gene designated SbPRP was isolated and characterized from 2-week-old soybean seedlings. It putatively encodes a bimodular protein similar to develop-mentally regulated proteins in other plant species. The deduced amino acid sequence consists of 126 amino acids with a distinct proline-rich domain (17 amino acids) and a long hydrophobically cysteine-rich domain (84 amino acids), plus a signal peptide of 25 amino acids in N terminal. SbPRP mRNA transcripts accumulated in an organ specific manner. It can be detected in leaves and epicotyls of soybean seedlings, whereas virtually expression signal of SbPRP was not detected in cotyledons, hypocotyls and roots. Further Northern hybridization suggested that SbPRP steady-state mRNA level accumulated differentially not only in response to salicylic acid, but to the inoculation of soybean mosaic virus Sa strain. Also it was responsive to drought treatment and salt (NaCI) stress. Therefore it is likely that SbPRP functions as a defense gene in soybean.

关键词： soybean (Glycine max), defense gene, salicylic acid, water deficit, bimodular protein.

CRISPR-based genetic control strategies for insect pests

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Journal of Integrative Agriculture 2023年第3期22卷 651-668页

作者： Ying YAN Roswitha A.AUMANN Irina HÄCKER Marc F.SCHETELIG Department of Insect biotechnology in plant Protection Institute for Insect BiotechnologyJustus-Liebig-University GiessenGiessen 35394Germany

Genetic control strategies such as the sterile insect technique have successfully fought insect pests worldwide. The CRISPR(clustered regularly interspaced short palindromic repeats) technology, together with high-quality genomic resources obtained in more and more species, greatly facilitates the development of novel genetic control insect strains that can be used in area-wide and species-specific pest control programs. Here, we review the research progress towards state-of-art CRISPR-based genetic control strategies, including gene drive, sex ratio distortion, CRISPRengineered genetic sexing strains, and precision-guided sterile insect technique. These strategies’ working mechanisms,potential resistance development mechanisms, and regulations are illustrated and discussed. In addition, recent developments such as stacked and conditional systems are introduced. We envision that the advances in genetic technology will continue to be one of the driving forces for developing the next generation of pest control strategies.

关键词： insect pest genetic control sterile insect technique gene drive genetic sexing strains CRISPR-Cas9

Expression of a High Mobility Group Protein Isolated from Cucumis sativus Affects the Germination of Arabidopsis thaliana under Abiotic Stress Conditions

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Journal of Integrative plant Biology 2008年第5期50卷 593-600页

作者： Ji Young Jang Kyung Jin Kwak Hunseung Kang Department of plant biotechnology Agricultural Plant Stress Research Center and Biotechnology Research InstituteCollege of Agriculture and Life SciencesChonnam National UniversityGwangju 500-757Korea

Although high mobility group B （HMGB） proteins have been identified from a variety of plant species, their importance and functional roles in plant responses to changing environmental conditions are largely unknown. Here, we investigated the functional roles of a CsHMGB isolated from cucumber （Cucumis sativus L.） in plant responses to environmental stimuli. Under normal growth conditions or when subjected to cold stress, no differences in plant growth were found between the wild-type and transgenic Arabidopsis thaliana overexpressing CsHMGB. By contrast, the transgenic Arabidopsis plants displayed retarded germination compared with the wild-type plants when grown under high salt or dehydration stress conditions. Germination of the transgenic plants was delayed by the addition of abscisic acid （ABA）, implying that CsHMGB affects germination through an ABA-dependent way. The expression of CsHMGB had affected only the germination stage, and CsHMGB did not affect the seedling growth of the transgenic plants under the stress conditions. The transcript levels of several germination-responsive genes were modulated by the expression of CsHMGB in Arabidopsis. Taken together, these results suggest that ectopic expression of a CsHMGB in Arabidopsis modulates the expression of several germinationresponsive genes, and thereby affects the germination of Arabidopsis plants under different stress conditions.

关键词： abiotic stress cucumber Cucumis sativus high mobility group protein transgenic Arabidopsis plant.

Genetic mapping of telomere-associated sequences in soybean (Glycine max)

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Progress in Natural Science:Materials International 2001年第5期11卷 33-39页

作者：刘峰张德水张劲松陈受宜 Beijing 100101 China Chinese Academy of Sciences institute of Genetics plant biotechnology Laboratory

Two telomere-associated sequences (TAS), named STAS8 and STAS10, were cloned from soybean genomic DNA using polymerase chain reaction (PCR) amplification. Southern analysis showed that they were sequences with moderate copy number in soybean genome. Sequence analysis demonstrated that STAS10 had tandemly arrayed consensus sequences of TTTAGGG and TTAGGG . The mapping of these two TAS was performed with a population of F8 re-combinant inbred line using restriction fragment length polymorphisms( RFLP). Seven out of nine polymorphic fragments were mapped to the most distal position of five linkage groups, Dla, F, G2, H and Q of soybean, and the other two loci were closely linked and mapped to two interstitial positions within linkage group Dla. The mapping of TAS in soybean is essential for completeness of a molecular genetic map of soybean.

关键词： soybean (Glycine max) telomere-associated sequence (TAS) genetic mapping.