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Two MADS-box proteins,AGL9 and AGL15,recruit the FIS-PRC2 complex to trigger the phase transition from endosperm proliferation to embryo development in Arabidopsis

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Molecular plant 2024年第7期17卷 1110-1128页

作者： Shen Zhang Devasantosh Mohanty Adnan Muzaffar Min Ni Department of plant and microbial biology University of Minnesota at Twin CitiesSaint PaulMinnesota MN 55108United States of AmericaUSA

Spatiotemporal regulation of gene expression by polycomb repressive complex 2(PRc2)is critical for animal and plant *** Arabidopsis fertilization independent seed(FIS)-PRC2 complex functions specifically during plant reproduction from gametogenesis to seed *** a double fertilization event,triploid endosperm proliferates early,followed by the growth of a diploid embryo,which replaces the endosperm in Arabidopsis and many *** genes critical for endosperm proliferation such as IKkU2 and MINI3 are activated after *** we report that two MADS-box AGAMOUS-LIKE(AGL)proteins associate with the key endosperm proliferation loci and recruit the FIS-PRC2 repressive complex at 4-5 days after pollination(DAP).Interestingly,AGL9 and AGL15 only accumulate toward the end of endosperm proliferation at 4-5 DAP and promote the deposition of H3K27me3 marks at key endosperm proliferation *** of AGL9 and AGL15 or overexpression of AGL9 or AGL15 significantly influence endosperm proliferation and ***-wide analysis with cleavage Under Targets and tagmentation(CUT&Tag)sequencing and RNA sequencing revealed the landscape of endosperm H3K27me3 marks and gene expression profiles in Col-0 and agl9 ***&Tag qPCR also demonstrated the occupancy of the two MADS-box proteins and FIS-PRC2 on a few representative target *** studies suggest that MADS-box proteins could potentially recruit PRc2 to regulate many other developmental processes in plants or even in fungi and animals.

关键词： AGL9 AGL15 embryo endosperm IKU2 MEDEA MINI3

Camellia sinensis CsMYB4a participates in regulation of stamen growth by interaction with auxin signaling transduction repressor CsAUX/IAA4

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The Crop Journal 2024年第1期12卷 188-201页

作者： Guoliang Ma Mingzhuo Li Yingling Wu Changjuan Jiang Yifan Chen Dawei Xing Yue Zhao Yajun Liu Xiaolan Jiang Tao Xia Liping Gao School of Life Science Anhui Agricultural UniversityHefei 230036AnhuiChina plant for Human Health institute Department of Plant and Microbial BiologyNorth Carolina State UniversityKannapolisNC 28081USA State Key Laboratory of Tea plant biology and Utilization/Key Laboratory of Tea biology and Tea Processing of Ministry of Agriculture/Anhui Provincial Laboratory of Tea plant biology and Utilization Anhui Agricultural UniversityHefei 230036AnhuiChina

Subgroup 4(Sg4)members of the R2R3-MYB are generally known as negative regulators of the phenylpropanoid pathway in *** previous research showed that a R2R3-MYB Sg4 member from Camellia sinensis(CsMYB4a)inhibits expression of some genes in the phenylpropanoid pathway,but its physiological function in the tea plant remained ***,CsMYB4a was found to be highly expressed in anther and filaments,and participated in regulating filament *** analysis and exogenous auxin treatment showed that the target of CsMYB4a might be the auxin signal ***/indole-3-acetic acid 4(AUX/IAA4),a repressor in auxin signal transduction,was detected from a yeast two-hybrid screen using CsMYB4a as *** silencing assays showed that both CsIAA4 and CsMYB4a regulate filament *** plants overexpressing CsIAA4 were insensitive to exogenous a-NAA,consistent with overexpression of ***-protein interaction experiments revealed that CsMYB4a interacts with N-terminal of CsIAA4 to prevent CsIAA4 *** out of the endogenous NtIAA4 gene,a CsIAA4 homolog,in tobacco alleviated filament growth inhibition and a-NAA insensitivity in plants overexpressing *** results strongly suggest that CsMYB4a works synergistically with CsIAA4 and participates in regulation of the auxin pathway in stamen.

关键词： AUX/IAA4 Auxin signaling CsMYB4a Subgroup 4 R2R3-MYB

Rational design of unrestricted pRN1 derivatives and their application in the construction of a dual plasmid vector system for Saccharolobus islandicus

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mLife 2024年第1期3卷 119-128页

作者： Pengpeng Zhao Xiaonan Bi Xiaoning Wang Xu Feng Yulong Shen Guanhua Yuan Qunxin She CRISPR and Archaea biology Research Center State Key Laboratory of Microbial Technology and Microbial Technology InstituteShandong UniversityQingdaoChina

Saccharolobus islandicus REY15A represents one of the very few archaeal models with versatile genetic tools,which include efficient genome editing,gene silencing,and robust protein expression ***,plasmid vectors constructed for this crenarchaeon thus far are based solely on the pRN2 cryptic *** this plasmid coexists with pRN1 in its original host,early attempts to test pRN1-based vectors consistently failed to yield any stable host-vector system for *** hypothesized that this failure could be due to the occurrence of CRISPR immunity against pRN1 in this *** identified a putative target sequence in orf904 encoding a putative replicase on pRN1(target N1).Mutated targets(N1a,N1b,and N1c)were then designed and tested for their capability to escape the host CRISPR immunity by using a plasmid inter-ference *** results revealed that the original target triggered CRISPR immunity in this archaeon,whereas all three mutated targets did not,indicating that all the designed target mutations evaded host *** mutated targets were then incorporated into orf904 individually,yielding corresponding mutated pRN1 backbones with which shuttle plasmids were constructed(pN1aSD,pN1bSD,and pN1cSD).*** transformation revealed that pN1aSD and pN1bSD were functional shuttle vectors,but pN1cSD lost the capability for *** results indicate that the missense mutations in the conserved helicase domain in pN1c inactivated the *** further showed that pRN1-based and pRN2-based vectors were stably maintained in the archaeal cells either alone or in combination,and this yielded a dual plasmid system for genetic study with this important archaeal model.

关键词： archaeal genetics CRISPR-Cas CRISPR escape mutations dual plasmid vectors Saccharolobus-E.coli shuttle vectors

m^(6)A modification of plant virus enables host recognition by NMD factors in plants

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Science China(Life Sciences) 2024年第1期67卷 161-174页

作者： Hao He Linhao Ge Yalin Chen Siwen Zhao Zhaolei Li Xueping Zhou Fangfang Li State Key Laboratory for biology of plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China State Key Laboratory of Rice biology Institute of BiotechnologyZhejiang UniversityHangzhou 310029China

N^(6)-methyladenosine(m^(6)A)is the most abundant eukaryotic mRNA modification and is involved in various biological *** evidence has implicated that m^(6)Amodification is an important anti-viral defense mechanism in mammals and plants,but it is largely unknown how m^(6)Aregulates viral infection in *** we report the dynamic changes and functional anatomy of m^(6)Ain Nicotiana benthamiana and Solanum lycopersicum during Pepino mosaic virus(PepMV)infection.m^(6)Amodification in the PepMV RNA genome is conserved in these two *** of the m^(6)Awriters,mRNA adenosine methylase A(MTA),and HAKAI inhibit the PepMV RNA accumulation accompanied by increased viral m^(6)Amodifications,whereas deficiency of these writers decreases the viral RNA m^(6)Alevels but enhances virus *** study reveals that the cytoplasmic YTH-domain family protein NbECT2A/2B/2C as m^(6)Areaders are involved in anti-viral ***-protein interactions indicate that NbECT2A/2B/2C interact with nonsense-mediated mRNA decay(NMD)-related proteins,including NbUPF3 and NbSMG7,but not with NbUPF1.m^(6)Amodification-mediated restriction to PepMV infection is dependent on NMD-related *** findings provide new insights into the functionality of m^(6)Aanti-viral activity and reveal a distinct immune response that NMD factors recognize the m^(6)Areaders-viral m^(6)ARNA complex for viral RNA degradation to limit virus infection in plants.

关键词： m^(6)A Pepino mosaic virus plant defense m^(6)A readers NMD factors

Two diversities meet in the rhizosphere:root specialized metabolites and microbiome

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Journal of Genetics and Genomics 2024年第5期51卷 467-478页

作者： Xiaochen Wang Jingying Zhang Xinjun Lu Yang Bai Guodong Wang State Key Laboratory of plant Genomics Institute of Genetics and Developmental BiologyThe Innovative Academy of Seed DesignChinese Academy of SciencesBeijing 100101China CAS-JIC Centre of Excellence for plant and microbial Science Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China Hainan Yazhou Bay Seed Laboratory SanyaHainan 572025China College of Advanced Agricultural Sciences Chinese Academy of SciencesBeijing 100049China

plants serve as rich repositories of diverse chemical compounds collectively referred to as specialized *** compounds are of importance for adaptive processes,including interactions with various microbes both beneficial and *** microbes as bioreactors,the chemical diversity undergoes dynamic changes when root-derived specialized metabolites(RSMs)and microbes encounter each other in the *** advancements in sequencing techniques and molecular biology tools have not only accelerated the elucidation of biosynthetic pathways of RSMs but also unveiled the significance of RSMs in plant-microbe *** this review,we provide a comprehensive description of the effects of RSMs on microbe assembly in the rhizosphere and the influence of corresponding microbial changes on plant health,incorporating the most up-to-date information ***,we highlight open questions that remain for a deeper understanding of and harnessing the potential of RSM-microbe interactions to enhance plant adaptation to the ***,we propose a pipeline for investigating the intricate associations between root exometabolites and the rhizomicrobiome.

关键词： Root specialized metabolites Microbiome plant-microbe interactions Rhizosphere Diversity

Chloroplast immunity: A cornerstone of plant defense

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Molecular plant 2024年第5期17卷 686-688页

作者： Jie Liu Pan Gong Ruobin Lu Rosa Lozano-Duran Xueping Zhou Fangfang Li State Key Laboratory for biology of plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100081China State Key Laboratory of Rice biology Institute of BiotechnologyZhejiang UniversityHangzhouZhejiang 310058China Department of plant Biochemistry Centre for Plant Molecular Biology(ZMBP)Eberhard Karls UniversityTubingen 72076Germany

Increasing evidence supports the notion that pattern-recognition-receptor-mediated immunity goes beyond plasma membrane-to-nucleus *** the findings of the pathways linking plasma membrane and chloroplasts and the functions of stromules and perinuclear chloroplast clustering(PCC)in plant defense,chloroplast immunity has emerged as a cornerstone of plant defense and a target of plant pathogens.

关键词： plant immunity corners

The competition between Bidens pilosa and Setaria viridis alters soil microbial composition and soil ecological function

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Journal of Integrative Agriculture 2024年第1期23卷 267-282页

作者： Qiao Li Jianying Guo Han Zhang Mengxin Zhao State Key Laboratory for biology of plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China College of Life Science Yangtze UniversityJingzhou 434025China

Bidens pilosa is recognized as one of the major invasive plants in *** invasion has been associated with significant losses in agriculture,forestry,husbandry,and *** ecosystems play an important role in alien plant *** within the soil act as intermediaries between plants and soil ecological functions,playing a role in regulating soil enzyme activities and nutrient *** the interactions between invasive plants,soil microorganisms,and soil ecological processes is vital for managing and mitigating the impacts of invasive species on the *** this study,we conducted a systematic analysis focusing on *** and Setaria viridis,a common native companion plant in the invaded *** simulate the invasion process of ***,we constructed homogeneous plots consisting of *** and *** grown separately as monocultures,as well as in *** rhizosphere and bulk soils were collected from the alien plant *** and the native plant *** order to focus on the soil ecological functional mechanisms that contribute to the successful invasion of ***,we analyzed the effects of *** on the composition of soil microbial communities and soil ecological *** results showed that the biomass of *** increased by 27.51% and that of *** was significantly reduced by 66.56%.The organic matter contents in the bulk and rhizosphere soils of *** were approximately 1.30 times those in the native plant *** TN and NO_(3)^(-)contents in the rhizosphere soil of *** were 1.30 to 2.71 times those in the native plant *** activities of acid phosphatase,alkaline phosphatase,and urease in the rhizosphere soil of *** were 1.98-2.25 times higher than in the native plant *** high-throughput sequencing of the16S rRNA gene,we found that *** altered the composition of the soil microbial ***,many genera in Actinobacteria and Proteobacteri

关键词： plant invasion Bidens pilosa soil microbial composition soil properties soil enzyme activities

The wheat sucrose synthase gene TaSus1 is a determinant of grain number per spike

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The Crop Journal 2024年第1期12卷 295-300页

作者： Liping Shen Lili Zhang Changbin Yin Xiaowan Xu Yangyang Liu Kuocheng Shen He Wu Zhiwen Sun Ke Wang Zhonghu He Xueyong Zhang Chenyang Hao Jian Hou Aoyue Bi Xuebo Zhao Daxing Xu Botao Ye Xuchang Yu Ziying Wang Danni Liu Yuanfeng Hao Fei Lu Zifeng Guo Key Laboratory of plant Molecular Physiology Institute of BotanyChinese Academy of SciencesBeijing 100093China China National Botanical Garden Beijing 100093China State Key Laboratory of plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyInnovative Academy of Seed DesignChinese Academy of SciencesBeijing 100101China institute of Crop Sciences Chinese Academy of Agricultural Sciences(CAAS)Beijing 100081China University of Chinese Academy of Sciences Beijing 100049China International Maize and Wheat Improvement Center(CIMMYT)China Office c/o CAASBeijing 100081China CAS-JIC Centre of Excellence for plant and microbial Science(CEPAMS) Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China

Some haplotypes of the sucrose synthase gene TaSus1 are associated with thousand-grain weight(TGW)in wheat(Triticum aestivum L.).However,no mutations have been identified within the gene to test this *** effects of TaSus1 on grain number per spike(GNS)also are largely *** previous genome-wide association study identified TaSus-A1 as a candidate gene controlling fertile spikelet number per spike(FSN).In the present study,we generated two independent mutants for the three TaSus1 homoeologs by CRISPR/Cas9-mediated genome *** triple mutants displayed lower FSN,GNS,grain number per spikelet(GNST),and TGW than wild-type *** 306 hexaploid wheat accessions,two single-nucleotide polymorphisms in TaSus-A1 contributed differently to *** of the two alleles into a wheat genetic background confirmed their *** alleles differed in geographical distribution among the accessions.

关键词： Domestication selection Fertile spikelet number per spike(FSN) Geographical differentiation Grain number per spike(GNS) TaSus1

RACK1A promotes hypocotyl elongation by scaffolding light signaling components in Arabidopsis

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Journal of Integrative plant biology 2024年第5期66卷 956-972页

作者： Yajuan Fu Wei Zhu Yeling Zhou Yujing Su Zhiyong Li Dayan Zhang Dong Zhang Jinyu Shen Jiansheng Liang Key Laboratory of Molecular Design for plant Cell Factory of Guangdong Higher Education institutes Department of BiologySchool of Life SciencesInstitute of Plant and Food SciencesSouthern University of Science and TechnologyShenzhen 518055China

plants deploy versatile scaffold proteins to intricately modulate complex cell *** these,RACK1A(Receptors for Activated C Kinase 1A)stands out as a multifaceted scaffold protein functioning as a central integrative hub for diverse signaling ***,the precise mechanisms by which RACK1A orchestrates signal transduction to optimize seedling development remain largely ***,we demonstrate that RACK1A facilitates hypocotyl elongation by functioning as a flexible platform that connects multiple key components of light signaling ***1A interacts with PHYTOCHROME INTERACTING FACTOR(PIF)3,enhances PIF3 binding to the promoter of BBX11 and down-regulates its ***,RACK1A associates with ELONGATED HYPOCOTYL 5(HY5)to repress HY5 biochemical activity toward target genes,ultimately contributing to hypocotyl *** darkness,RACK1A is targeted by CONSTITUTIVELY PHOTOMORPHOGENIC(COP)1 upon phosphorylation and subjected to COP1-mediated degradation via the 26 S proteasome *** findings provide new insights into how plants utilize scaffold proteins to regulate hypocotyl elongation,ensuring proper skoto-and photo-morphogenic development.

关键词： Arabidopsis hypocotyl development light signaling components RACK1 scaffoldprotein