检索结果-南通市图书馆

Toward Understanding the Stem-Cell Origin and Molecular Regulation of Rice Tillering

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of genetics and genomics 2015年第2期42卷 47-48页

作者： Chuanyou Li State Key Laboratory of Plant genomics and National Center for Plant Gene Research (Beijing) Institute of Genetics and Developmental BiologyChinese Academy of Sciences

Rice tiller is a specialized grain-bearing branch that contrib- utes greatly to grain production. Therefore, rice tillering is an important agronomic trait and provides a model system for the study of branching in monocots. Owing its importance both to agriculture and to fundamental science, much attention has been given to understand the molecular mechanisms under- lying rice tillering.

关键词： MOC Toward Understanding the Stem-Cell Origin and Molecular Regulation of Rice Tillering

Understanding the pathophysiology of postpartum psychosis: Challenges and new approaches

在线全文

学校读者我要写书评

暂无评论

World Journal of Psychiatry 2017年第2期7卷 77-88页

作者： William Davies medical Research Council Centre for Neuro-psychiatric genetics and genomics and Division of Psychological Medicine and Clinical Neurosciences School of Medicine Cardiff University School of Psychology Cardiff University Neuroscience and Mental Health Research institute Cardiff University

Postpartum psychosis is a severe psychiatric condition which affects 1-2 of every 1000 mothers shortly after childbirth. Whilst there is convincing evidence that the condition is precipitated by a complex combination of biological and environmental factors, as yet the pathophysiological mechanisms remain extremely poorly defined. Here, I critically review approaches that have been, or are being, employed to identify and characterise such mechanisms; I also review a recent animal model approach, and describe a novel biological risk model that it suggests. Clarification of biological risk mechanisms underlying disorder risk should permit the identification of relevant predictive biomarkers which will ensure that "at risk" subjects receive prompt clinical intervention if required.

关键词： CCN3 Immune system Steroid sulfatase Nephroblastoma-overexpressed Mouse Animal model Risk factor

Putative Chitin Synthases from Branchiostoma floridae Show Extracellular Matrix-related Domains and Mosaic Structures

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

genomics, Proteomics & Bioinformatics 2012年第4期10卷 197-207页

作者： Gea Guerriero Fungal genetics and genomics Unit Department of Applied Genetics and Cell BiologyUniversity of Natural Resources and Life Science ViennaUniversity and Research Center Campus Tulln-TechnopolKonrad Lorenz Strasse 24A-3430 TullnAustria

The transition from unicellular to multicellular life forms requires the development of a specialized structural component, the extracel- lular matrix （ECM）. In Metazoans, there are two main supportive systems, which are based on chitin and collagen/hyaluronan, respec- tively. Chitin is the major constituent of fungal cell walls and arthropod exoskeleton. However, presence of chitin/chitooligosaccharides has been reported in lower chordates and during specific stages of vertebrate development. In this study, the occurrence of chitin syn- thases （CHSs） was investigated with a bioinformatics approach in the cephalochordate Branchiostomafloridae, in which the presence of chitin was initially reported in the skeletal rods of the pharyngeal gill basket. Twelve genes coding for proteins containing conserved amino acid residues of processive glycosyltransferases from GT2 family were found and 10 of them display mosaic structures with novel domains never reported previously in a chitin synthase. In particular, the presence of a discoidin （DS） and a sterile alpha motif （SAM） domain was found in nine identified proteins. Sequence analyses and homology modelling suggest that these domains might interact with the extracellular matrix and mediate protein-protein interaction. The multi-domain putative chitin synthases from B. floridae constitute an emblematic example of the explosion of domain innovation and shuffling which predate Metazoans.

关键词： Branchiostomafloridae Chitin synthase Discoidin domain Extracellular matrix

Praseodymium metal nanorods as a 5-fluorouracil carrier

在线全文

学校读者我要写书评

暂无评论

Journal of Rare Earths 2024年第7期42卷 1328-1336,I0005页

作者： Varnitha Manikantan Govindaraj Sri Varalakshmi Ujma Ashapak Tamboli Archana Sumohan Pillai Aleyamma Alexander Anushka Lucas Bose Allben Akash Israel V.M.V.Enoch Centre for Nanoscience and genomics Karunya Institute of Technology and Sciences(Deemed University)Coimbatore641114Tamil NaduIndia Department of applied Chemistry Karunya Institute of Technology and Sciences(Deemed University)Coimbatore641114Tamil NaduIndia

Interest in metal nanoparticles as nanoscale drug-carrying vehicles has been *** such an aspect,lanthanide metal nanoparticles are particularly ***,we report praseodymium nanoparticles as a novel anticancer therapeutic transporting *** material was made as a composite with the supramolecular host polymer,poly-β-*** praseodymium nanorods were characterized using standard analytical techniques,including X-ray diffraction(XRD),X-ray photoelectron spectroscopy(XPS),and thermogravimetric analysis(TGA).In addition,they were imaged using transmission electron *** nanostructures are a long rod-shaped material with 2.0±0.1μm in length and 230±10 nm in *** material shows a saturation magnetization value above 30 emu/g with a coercivity value of 130 *** nanocarrier is freighted with the drug 5-fluorouracil,and the encapsulation efficiency is 76.09%.The drug release is continuous from the nanocarrier(polymer-containing praseodymium nanoparticles)and proceeds for above 250 *** in vitro cytotoxicities of the blank nanocarrier and the 5-fluorouracil-loaded one are deduced by testing them on MCF-7 and Vero(non-cancerous)cell *** cytotoxicity on the cancerous cell lines is observed,whereas the non-cancerous cells are *** IC_(50)values on the cell lines are reported.

关键词： Metal nanoparticles Praseodymium Poly-cyclodextrin Drug delivery Anticancer activity Rare earths

Cell Polarity and Development

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2013年第9期55卷 786-788页

作者： Remko Offringa Jürgen Kleine‐Vehn Molecular and Developmental genetics institute Biology Leiden Leiden University Sylvius Laboratory Universitt für Bodenkultur Wien (BOKU) institute of applied genetics and Cell Biology (IAGZ)

All ceiis show some degree of poiarity, either by asymmetrically distributed membrane or cytosolic components. Even in bacterial cells that do not have the eukaryotic membrane compartmentalization of the cytoplasm, proteins can be localized at specific areas. In rod-shaped bacteria, many processes such as signaling, flagella formation, and DNA uptake occur at the cell poles. In addition,

关键词： Cell Polarity Membrane Curvature

Cell-wall Invertases from Rice are Differentially Expressed in Caryopsis during the Grain Filling Stage

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2008年第4期50卷 466-474页

作者： Yong-Qin Wang Xiao-Li Wei Hong-Lin Xu Cheng-Lin Chai Kun Meng Hong-Li Zhai Ai-Jun Sun Yong-Gang Peng Bin Wu Gui-Fang Xiao Zhen Zhu State Key Laboratory of Plant genomics Institute of Genetics and Developmental Biology the Chinese Academy of Sciences Beijing 100101 China Graduate School of the Chinese Academy of Sciences Beijing 100049 China

Cell-wall invertase plays an important role in sucrose partitioning between source and sink organs in higher plants. To investigate the role of cell-wall invertases for seed development in rice （Oryza sativa L.）, cDNAs of three putative cell- wall invertase genes OsCIN1, OsCIN2 and OsCIN3 were isolated. Semi-quantitative reverse transcription-polymerase chain reaction analysis revealed different expression patterns of the three genes in various rice tissues/organs. In developing caryopses, they exhibited similar temporal expression patterns, expressed highly at the early and middle grain filling stages and gradually declined to low levels afterward. However, the spatial expression patterns of them were very different, with OsCIN1 primarily expressed in the caryopsis coat, OsCIN2 in embryo and endosperm, and OsCIN3 in embryo. Further RNA in situ hybridization analysis revealed that a strong signal of OsCIN2 mRNA was detected in the vascular parenchyma surrounding the xylem of the chalazal vein and the aleurone layer, whereas OsCIN3 transcript was strongly detected in the vascular parenchyma surrounding the phloem of the chalazal vein, cross-cells, the aleurone layer and the nuceUar tissue. These data indicate that the three cell-wall invertase genes play complementary/synergetic roles in assimilate unloading during the grain filling stage. In addition, the cell type-specific expression patterns of OsCIN3 in source leaf blades and anthers were also investigated, and its corresponding physiological roles were discussed.

关键词： cell-wall invertase gene expression Oryza sativa RNA in situ hybridization seed development.

Cloning and Expression Analysis of Rice Sucrose Transporter Genes OsSUT2M and OsSUT5Z

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2008年第1期50卷 62-75页

作者： Ai-Jun Sun Hong-Lin Xu Wan-Kui Gong Hong-Li Zhai Kun Meng Yong-Qin Wang Xiao-Li Wei Gui-Fang Xiao Zhen Zhu State Key Laboratory of Plant genomics Institute of Genetics and Developmental Biology the Chinese Academy of Sciences Beijing 100101 China Graduate School of the Chinese Academy of Sciences Beijing 100049 China

Two sucrose transporter （SUT） cDNAs, OsSUT2M and OsSUT5Z, were isolated from rice （Oryza sativa L.） by reverse transcription polymerase chain reaction （RT-PCR）. Sequencing results indicate they are 1 531 bp and 1 635 bp in length including complete open reading frame 1 506bp and 1 608 bp, which encode 502 amino acids and 536 amino acids, respectively. The TopPred program suggested that both sucrose transporter proteins, OsSUT2M and OsSUT5Z, consist of potentially 12 transmembrane domains. Semi-quantitative RT-PCR was carried out to investigate the gene expression patterns of OsSUT2M and OsSUT5Z. In vegetative organs, transcripts of OsSUT2M were higher in source leaf blades than in other organs at the same development stage, whereas transcripts of OsSUT5Z were less traceable in all organs investigated. In reproductive organs, both transcripts of these two genes were high in panicles from the booting stage to 7 days after flowering （DAF） and then sharply declined. The potential physiology functions of these two sucrose transporters are discussed.

关键词： cDNA cloning expression pattern OsSUT2M OsSUT5Z rice sucrose transporter.

Ubiquitination in Abscisic Acid-Related Pathway

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2007年第1期49卷 87-93页

作者： Yi-Yue Zhang Qi Xie State Key Laboratory of Plant genomics Institute of Genetics and Developmental Biology the Chinese Academy of Sciences Beijing 100101 China Graduate School of the Chinese Academy of Sciences Beijing 100039 China

Ubiquitination is emerging as a tight regulatory mechanism that is necessary for all aspects of development and survival of all eukaryotes. Recent genomic and genetic analysis in Arabidopsis suggests that ubiquitination may also play important roles in plant response to the phytohormone abscisic acid （ABA）. Many components of the ubiquitination pathway, such as ubiquitin-conjugating enzyme E2, ubiquitin ligase E3 and components of the proteasome, have been identified or predicted to be essential in ABA biosynthesis, catabolism and signaling. In addition, the ubiquitination-related pathway, sumoylation, is also involved in ABA signaling. We summarize in this report recent developments to elucidate their roles in the ABA-related pathway.

关键词： ABA E3 ligase proteolysis SUMO ubiquitination ubiquitin-conjugating enzyme (E2)

Highly Fluorescent Dye-Doped Silica Nanoparticles Increase Flow Cytometry Sensitivity for Cancer Cell Monitoring

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nano Research 2009年第6期2卷 448-461页

作者： M.-Carmen Estévez Meghan B.OʼDonoghue Xiaolan Chen Weihong Tan Center for Research at the Bio/Nano Interface Departments of Chemistry and Department of Physiology and Functional GenomicsShands Cancer Center and UF Genetics InstituteMcKnight Brain InstituteUniversity of FloridaGainesvilleFlorida 32611-7200USA

Early and accurate diagnosis and treatment of cancer depend on rapid,sensitive,and selective detection of tumor *** diagnosis of cancers,especially leukemia,relies on histology and fl ow cytometry using single dye-labeled ***,this combination may not lead to high signal output,which can hinder detection,especially when the probes have relatively weak affi nities or when the receptor is expressed in a low concentration on the target cell *** solve these problems,we have developed a novel method for sensitive and rapid detection of cancer cells using dye-doped silica nanoparticles(NPs)which increases detection sensitivity in fl ow cytometry analyses between 10-and 100-fold compared to standard *** NPs are~60 nm in size and can encapsulate thousands of individual dye molecules within their *** have extensively investigated surface modifi cation strategies in order to make the NPs suitable for selective detection of cancer cells using fl ow *** NPs are functionalized with polyethylene glycol(PEG)to prevent nonspecifi c interactions and with neutravidin to allow universal binding with biotinylated *** virtue of their reliable and selective detection of target cancer cells,these NPs have demonstrated their promising usefulness in conventional fl ow ***,they have shown low background signal,high signal enhancement,and effi cient functionalization,either with antibody-or aptamer-targeting moieties.

关键词： Silica nanoparticle flow cytometry aptamer cell detection polyethylene glycol fl uorophore