Cell-wall Invertases from Rice are Differentially Expressed in Caryopsis during the Grain Filling Stage

作     者：Yong-Qin Wang Xiao-Li Wei Hong-Lin Xu Cheng-Lin Chai Kun Meng Hong-Li Zhai Ai-Jun Sun Yong-Gang Peng Bin Wu Gui-Fang Xiao Zhen Zhu 

作者机构：State Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biology the Chinese Academy of Sciences Beijing 100101 China Graduate School of the Chinese Academy of Sciences Beijing 100049 China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2008年第50卷第4期

页      面：466-474页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

基　　金：the State Key Basic Research and Development Plan ofChina (2004CB720406) the Innovation Foundation of the Chinese Academyof Sciences (KSCX2-SW-306 and KSCX1-SW-03) the Program for Strategic Scientific Alliances between China and the Netherlands (KNAW-PSA, 04-PSA-BD-04 for P.B.F.O., KNAW-CEP and 04CDP022 for Y.X.) 

主　　题：cell-wall invertase gene expression Oryza sativa RNA in situ hybridization seed development. 

摘      要：Cell-wall invertase plays an important role in sucrose partitioning between source and sink organs in higher plants. To investigate the role of cell-wall invertases for seed development in rice （Oryza sativa L.）, cDNAs of three putative cell- wall invertase genes OsCIN1, OsCIN2 and OsCIN3 were isolated. Semi-quantitative reverse transcription-polymerase chain reaction analysis revealed different expression patterns of the three genes in various rice tissues/organs. In developing caryopses, they exhibited similar temporal expression patterns, expressed highly at the early and middle grain filling stages and gradually declined to low levels afterward. However, the spatial expression patterns of them were very different, with OsCIN1 primarily expressed in the caryopsis coat, OsCIN2 in embryo and endosperm, and OsCIN3 in embryo. Further RNA in situ hybridization analysis revealed that a strong signal of OsCIN2 mRNA was detected in the vascular parenchyma surrounding the xylem of the chalazal vein and the aleurone layer, whereas OsCIN3 transcript was strongly detected in the vascular parenchyma surrounding the phloem of the chalazal vein, cross-cells, the aleurone layer and the nuceUar tissue. These data indicate that the three cell-wall invertase genes play complementary/synergetic roles in assimilate unloading during the grain filling stage. In addition, the cell type-specific expression patterns of OsCIN3 in source leaf blades and anthers were also investigated, and its corresponding physiological roles were discussed.