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Fluorescence color diversity of great barrier reef corals

Journal of Innovative Optical Health Sciences 2015年第4期8卷 58-68页

作者： Grigory Lapshin Anya Salih Peter Kolosov Maria Golovkina Yuri Zavorotnyi Tatyana Ivashina Leonid Vinokurov Victor Bagratashvili Alexander Savitsky Physical Biochemistry Lab INBI RASLeninsky Prospekt 33Build.2 Moscow 119071Russia Faculty of Bioengineering and Bioinformatics Moscow State University Leninskiye Gory 1-73 MSU GSP-1 Moscow 119991Russia Confocal Bio-Imaging Facility(CBIF) Division Deputy Vice-Chancellor(Research&Development)University of Western SydneyLocked Bag 1797 Penrith South DCNSW 1797Australia institute of higher nervous activity and neurophysiology The Russian Academy of Sciences 5A Butlerova St.Moscow 117485Russia Skobeltsyn institute of Nuclear Physics Moscow State University 1(2)Leninskie Gory GSP-1Moscow 119991Russia Molecular Microbiology Lab Skryabin Institute of Biochemistry and Physiology of Micro-organisms RAS Prospect Nauki 5 Pushchino142290 Moscow RegionRussia Group of Bioengineering of Reporter Proteins Branch of the Shemyakin-Ovchinnikov institute of Bioorganic Chemistry of the Russian Academy of Sciences Prospekt Nauki 65 Pushchino 142290 Moscow RegionRussia institute of Laser and Information Technologies RAS 2 Pionerskaya TroitskMoscow 142190Russia

A group of variously colored proteins belonging to the green fAuorescent protein(GFP)family are responsible for coloring coral *** of the Great Barrier Reef were studied with the custom-built fiber laser fluorescence *** analysis showed that most of the excarmined corals contained multiple fuorescent peaks ranging from 470 to *** obser-vation was attributed to the presence of multiple genes of GFP-like proteins in a single coral,as well as by the photo-induced post-translational modifcations of certain GFP-like *** isolated a novel photo-convertible fuorescent protein(FP)from one of the tested ***:propose that two processes may explain the observed diversity of the fuorescent spectra in corals:(1)dark post-translational modifcation(maturation),and(2)color photo-conversion of certain maturated proteins in response to sunlight.

关键词： Coral fuorescence GFP like proteins fuorophores Kaede

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The Effect of Ethanol on the Neuronal Subserving of Behavior in the Hippocampus

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Journal of Behavioral and Brain Science 2013年第1期3卷 107-130页

作者： Yuri I. Alexandrov Yuri V. Grinchenko Diana G. Shevchenko Robert G. Averkin Valentina N. Matz Seppo Laukka Mikko Sams Brain and Mind Laboratory Department of Biomedical Engineering and Computational Science (BECS) Aalto University School of Science Helsinki Finland Department of Psychophysiology Faculty of Psychology State Academical University of Humanitarian Sciences Moscow Russia Laboratory of Morphology of the Central nervous System Institute of Higher Nervous Activity and Neurophysiology Russian Academy of Sciences Moscow Russia Learning Research Laboratory (LearnLab) University of Oulu Oulu Finland V. B. Svyrkov Laboratory of Neural Bases of Mind Institute of Psychology Russian Academy of Sciences Moscow Russia V. B. Svyrkov Laboratory of Neural Bases of Mind Institute of Psychology Russian Academy of Sciences Moscow Russia

We have previously shown that both acute and chronic ethanol treatment depresses neural activity, specifically in the cingulate cortex. Minor influences were found in the motor cortex. The acute effect of ethanol in the hippocampus was intermediate to those in the cingulate and motor cortices. In the present study, we concentrate on the chronic effects of ethanol on the hippocampus. We demonstrate how the neuronal activity underlying food-acquisition behavior is modified after chronic ethanol treatment, and how the hippocampus subserves formation of newly-formed alcohol-acquisition behavior. Neuronal activity in CA1 was more sensitive to chronic ethanol than the Dg area. Acute administration of ethanol had a normalizing effect on the chronically-treated animals: their performance and the hippocampal neural activity approached a normal range. The sets of neurons involved in food-acquisition behavior formed before chronic ethanol treatment, and those involved in alcohol-acquisition behavior formed after treatment significantly overlapped supporting the view that the neuronal mechanisms of pre-existing behavior provide the basis for the formation of new behavior. Additionally, we also discovered alcohol-acquisition selective neurons. Assuming that the formation of new neuronal specializations underlies learning, we believe that alcohol-selective neurons are specialized during the formation of alcohol-acquisition behavior. Our data demonstrate several new findings on the effect of acute and chronic ethanol on hippocampus activity, and how the neuronal activity relates to behavior before and after ethanol treatment.

关键词： Neuronal activity Learning Memory Place Cells CA1 Dg Alcohol Morphology

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Antisense MMP-9 RNA inhibits malignant glioma cell growth in vitro and in vivo

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Neuroscience Bulletin 2013年第1期29卷 83-93页

作者： Cuiyun Sun Qian Wang Hongxu Zhou Shizhu Yu Alain R. Simard Chunsheng Kang Yanyan Li Yanling Kong Tongling An Yanjun Wen Fudong Shi Junwei Hao Department of Neuropathology Tianjin Neurological Institute Tianjin Medical University General Hospital Key Laboratory of Post-trauma Neuro-repair and Regeneration in the Central nervous System Ministry of Education Tianjin Key Laboratory of Injuries Variations and Regeneration of the Nervous System Département de Chimie et Biochimie Université de Moncton Moncton New Brunswick E1A 3E9 Canada Department of Neurology Tianjin Neurological Institute Tianjin Medical University General Hospital

The matrix-degrading metalloproteinases （MMPs）, particularly MMP-9, play important roles in the pathogenesis and development of malignant gliomas. In the present study, the oncogenic role of MMP-9 in malignant glioma cells was investigated via antisense RNA blockade in vitro and in vivo. TJ905 malignant glioma cells were transfected with pcDNA3.0 vector expressing antisense MMP-9 RNA （pcDNA-AS-MMP9）, which significantly decreased MMP-9 expression, and cell proliferation was assessed. For in vivo studies, U251 cells, a human malignant glioma cell line, were implanted subcutaneously into 4-to 6-week-old BALB/c nude mice. The mice bearing well-established U251 gliomas were treated with intratumoral pcDNA-AS-MMP9-Lipofectamine complex （AS-MMP-9-treated group）, subcutaneous injection of endostatin （endostatin-treated group）, or both （combined therapy group）. Mice treated with pcDNA （empty vector）-Lipofectamine served as the control group. Four or eight weeks later, the volume and weight of tumor, MMP-9 expression, microvessel density and proliferative activity were assayed. We demonstrate that pcDNA-AS-MMP9 significantly decreased MMP-9 expression and inhibited glioma cell proliferation. Volume and weight of tumor, MMP-9 expression, microvessel density and proliferative activity in the antisense-MMP-9-treated and therapeutic alliance groups were significantly lower than those in the control group. The results suggest that MMP-9 not only promotes malignant glioma cell invasiveness, but also affects tumor cell proliferation. Blocking the expression of MMP-9 with antisense RNA substantially suppresses the malignant phenotype of glioma cells, and thus can be used as an effective therapeutic strategy for malignant gliomas.

关键词： matrix-degrading metalloproteinase 9 antisense MMP-9 RNA cell proliferation malignant glioma cells

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Endogenous level of TIGAR in brain is associated with vulnerability of neurons to ischemic injury

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Neuroscience Bulletin 2015年第5期31卷 527-540页

作者： Lijuan Cao Jieyu Chen Mei Li Yuan-Yuan Qin Meiling Sun Rui Sheng Feng Han Guanghui Wang Zheng-Hong Qin Department of Pharmacology and Laboratory of Aging and nervous Diseases Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases College of Pharmaceutical Science Soochow University institute of Pharmacology Toxicology and Biochemical Pharmaceutics Zhejiang University Department of Pharmacology and Laboratory of Molecular Neuropathology Soochow University School of Pharmaceutical Science

In previous studies,we showed that TP53-induced glycolysis and apoptosis regulator（TIGAR） protects neurons against ischemic brain *** the present study,we investigated the developmental changes of TIGAR level in mouse brain and the correlation of TIGAR expression with the vulnerability of neurons to ischemic *** found that the TIGAR level was high in the embryonic stage,dropped at birth,partially recovered in the early postnatal period,and then continued to decline to a lower level in early adult and aged *** TIGAR expression was higher after ischemia/reperfusion in mouse brain 8and 12 weeks after ***-week-old mice had smaller infarct volumes,lower neurological scores,and lower mortality rates after ischemia than 8- and12-week-old *** expression also increased in response to oxygen glucose deprivation（OGD）/reoxygenation insult or H_2O_2 treatment in cultured primary neurons from different embryonic stages（E16 and E20）.The neurons cultured from the early embryonic period had a greater resistance to OGD and oxidative *** TIGAR levels correlated with higher pentose phosphate pathway activity and less oxidative *** mice and more mature neurons had more severe DNA and mitochondrial damage than younger mice and less mature neurons in response to ischemia/reperfusion or OGD/reoxygenation *** of cultured neurons with nicotinamide adenine dinuclectide phosphate（NADPH） significantly reduced ischemic *** results suggest that TIGAR expression changes during development and its expression level may be correlated with the vulnerability of neurons to ischemic injury.

关键词： TIGAR NADPH ischemia OGD H2O2

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Longitudinal quantitative electroencephalographic study in mono-hemispheric stroke patients

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Neural Regeneration Research 2019年第7期14卷 1237-1246页

作者： Filippo Zappasodi Franca Tecchio Laura Marzetti Vittorio Pizzella Vincenzo Di Lazzaro Giovanni Assenza Department of Neuroscience Imaging and Clinical Sciences and Institute for Advanced Biomedical Imaging"G.D'Annunzio" University Laboratory of Electrophysiology for Translational NeuroScience(LET’S) ISTC-CNRand Fondazione Policlinico Gemelli IRCCS Unit of Neurology NeurophysiologyNeurobiologyDepartment of MedicineUniversità Campus Bio-Medico di Roma

The identification of individual factors modulating clinical recovery after a stroke is fundamental to personalize the therapeutic intervention to enhance the final clinical *** this framework,electrophysiological factors are promising since are more directly related to neuroplasticity,which supports recovery in stroke patients,than neurovascular *** this retrospective observational study,we investigated brain neuronal activity assessed via spectral features and Higuchi's fractal dimension(HFD) of electroencephalographic signals in acute phase(2–10 days from symptom onset,T0) and sub-acute phase(2.5 months,T1) in 24 patients affected by unilateral middle cerebral artery *** assessment of the clinical deficits was performed using the National institutes of Health Stroke Scale(NIHSS),together with the effective recovery calculated as the ratio between difference of NIHSS at T0 and T1 over the NIHSS value at *** observed that delta and alpha band electroencephalographic signal power changed between the two phases in both the hemispheres ipsilateral(ILH) and contralateral(CHL) to the ***,at T0,bilateral higher delta band power correlated with worse clinical conditions(Spearman's rs = 0.460,P = 0.027 for ILH and rs = 0.508,P = 0.013 for CLH),whereas at T1 this occurred only for delta power in ILH(rs = 0.411,P = 0.046) and not for ***-hemispheric difference(ILH ***) of alpha power in patients was lower at T0 than at T1(P = 0.020).HFD at T0 was lower than at T1(P = 0.005),and at both phases,ILH HFD was lower than CLH HFD(P = 0.020).These data suggest that inter-hemispheric low band asymmetry and fractal dimension changes from the acute to the sub-acute phase are sensitive to neuroplasticity processes which subtend clinical *** study protocol was approved by the Bioethical Committee of Ospedale San Giovanni Calibita Fatebenefretelli(No.40/2011) on July 14,2011.

关键词： mono-hemispheric stroke delta band fractal dimension inter-hemispheric asymmetries EEG plasticity

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Effects of Neonatal Undernutrition on Development of the Dorsolateral Prefrontal Cortex Pyramidal Cells in the Rat

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Journal of Behavioral and Brain Science 2014年第1期4卷 49-57页

作者： Carmen Torrero Mirelta Regalado Lorena Rubio Manuel Salas Department of Developmental Neurobiology and neurophysiology Institute of Neurobiology National Autonomous University of Mexico Campus UNAM Juriquilla Queretaro Mexico

The dorsolateral prefrontal cortex (dlPFC) of the rat plays a role in the encoding of neuronal signals involved in conflict-induced behavioral adjustment, working memory, planning and executive abilities, attentional control and other cognitive responses. In altricial species, early perinatal undernutrition interferes with the morphofunctional organization of a number of central nervous system (CNS) structures including the prefrontal cortex. The effects of neonatal undernutrition on dendritic arbor density, perikaryon measurements, and the number of spines (detected by rapid-Golgi) of basilar dendritic segments in layer III pyramidal neurons of the dlPFC were examined in male Wistar rats on postnatal (PDs) 12, 20, and 30. In the underfed (U) subjects the distal portions of the dendritic arbors had a consistent hipoplasia, mainly on PD 30, with reduced cross sectional area, perimeter, and spine densities on the basilar dendrites on all days studied. Thus, the alterations of the dlPFC pyramidal neurons may interfere with the plastic synaptic activity and cognitive performance of rats subjected to the stress of early underfeeding. Characterizing these anatomical alterations may help to understand the disrupted cognitive processes associated with neonatal undernutrition.

关键词： Neonatal Undernutrition Prefrontal Cortex Development Stressed Rats

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Networks in motion - from ion channels to behaviour

Networks in motion - from ion channels to behaviour

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中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Sten Grillner Nobel institute for neurophysiology Department of NeuroscienceStockholm Brain InstituteKarolinska InstitutetSE-171 77 Stockholm

＜正＞In all vertebrates from primates including man to fish and lamprey,the basic neuronal organization controlling patterns of motor behaviour such as locomotion,breathing,and posture are organized in a similar or identical *** networks form a motor infrastructure used to initiate all the movements that each species can *** networks in turn can be selected through subpopulations of cells in the basal ganglia,which play an important role for the selection of motor *** networks generating the locomotor movements are located in the spinal *** are responsible for the sequential activation of the different limb muscle in each locomotor *** activity level is controlled by locomotor command centres in the brainstem,conserved throughout the vertebrate *** elucidate the intrinsic function of these pattern generating networks and command systems we have developed the lamprey nervous system as an experimental *** now understand the cellular bases of the brainstem spinal cord networks generating goal- directed locomotion and control of body *** detailed synaptic interaction has been identified,as well as the network contribution of subtypes of ion channels.A major tool has been biologically realistic large scale computing.

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A conserved region in the 3' untranslated region of the human LIMK1 gene is critical for proper expression of LIMK1 at the post-transcriptional level

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Neuroscience Bulletin 2013年第3期29卷 348-354页

作者： Guang-Fei Deng Shu-Jing Liu Xun-Sha Sun Wei-Wen Sun Qi-Hua Zhao Wei-Ping Liao Yong-Hong Yi Yue-Sheng Long Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China Institute of Neuroscience and the Second Affiliated Hospital of Guangzhou Medical University Key Laboratory for Major Obstetric Diseases of Guangdong Province and Key Laboratory of Reproduction and Genetics of Guangdong higher Education institutes Guangzhou Medical University

LIM kinase 1 （LIMK1）, a cytosolic serine/threonine kinase, regulates actin filament dynamics and reorganization and is involved in neuronal development and brain function. Abnormal expression of LIMK1 is associated with several neurological disorders. In this study, we performed a conservation analysis using Vector NTI （8.0） software. The dualluciferase reporter assay and real-time quantitative RT-PCR were used to assess the protein and mRNA levels of the reporter gene, respectively. We found that a region ranging from nt ＋884 to ＋966 in the human LIMK1 3＇ untranslated region （UTR） was highly conserved in the mouse Limkl 3＇ UTR and formed a structure containing several loops and stems. Luciferase assay showed that the relative luciferase activity of the mutated construct with the conserved region deleted, pGL4-hLIMK1-3U-M, in SH-SY5Y and HEK-293 cells was only -60% of that of the wild-type construct pGL4-hLIMK1-3U, indicating that the conserved region is critical for the reporter gene expression. Real-time quantitative RT-PCR analysis demonstrated that the relative Luc2 mRNA levels in SH-SY5Y and HEK293 cells transfected with pGL4-hLIMK1-3U-M decreased to50% of that in cells transfected with pGL4-hLIMK1- 3U, suggesting an important role of the conserved region in maintaining Luc2 mRNA stability. Our study suggests that the conserved region in the LIMK1 3＇ UTR is involved in regulating LIMK1 expression at the post-transcriptional level, which may help reveal the mechanism underlying the regulation of LIMK1 expression in the central nervous system and explore the relationship between the 3＇-UTR mutant and neuroloqical disorders.

关键词： LIM kinase 1 region post-transcriptsecondary structurethree-prime untranslatedonal regulation RNA

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Molecular Docking Studies of Alpinia galanga Phytoconstituents for Psychostimulant activity

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Advances in Biological Chemistry 2018年第4期8卷 69-80页

作者： Santhy Sivanandan Surekha Pimple Avinashilingam institute for Home Science and higher Education for Women CoimbatoreIndia Enovate Biolife WilmingtonDEUSA

Dopamine is a neurotransmitter responsible for sending signals from the central nervous system. It allows human beings to stay attentive and focused. Caffeine, the most widely consumed psychoactive substance in the world, is known to improve alertness by enhancing dopamine signaling in the brain. EnXtra®, an Alpinia galanga extract has been clinically proven to promote alertness however the mechanism for such action required elucidation. The current study was designed to explore the mechanism for the neurocognitive enhancing property of EnXtra®by the in-silico interaction of its potential compounds with various targets involved in such process namely Dopamine and Acetylcholinesterase (AchE). As evident by the outcomes of the study, active compounds of EnXtra®can block the dopamine reuptake thereby increasing the dopamine levels which further can enhance the visuospatial performance and mental clarity, leading to improved mental alertness. At the same time, its strong effect on Acetylcholinesterase receptors is indicative of its nootropic potential.

关键词： EnXtra^■ Caffeine Dopamine Enovate Biolife Energy Drink Cognitive In-Silico

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Serotonylation: A novel histone H3 marker

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Signal Transduction and Targeted Therapy 2019年第1期4卷 548-549页

作者： Lin Fu Lingqiang Zhang institute of Chronic Disease Qingdao Municipal HospitalQingdao University266000 QingdaoChina State Key Laboratory of Proteomics National Center of Protein Sciences(Beijing)Beijing Institute of Lifeomics100850 BeijingChina

A recent study by Farrelly,*** *** in Nature showed that the serotonylation of glutamine at the Q5 site in histone H3 promotes the recruitment of TFIID in collaboration with adjacent H3K4 trimethylation *** posttranslational modifications(PTMs)of histones are associated with diverse biological processes through dynamically modulating chromatin structure and the transcriptional activity of genes *** regulations are an integral part of brain functions ranging from the development of the nervous system beyond basic neurological functions to higher-order cognitive processes 3.

关键词： roton system trimethyl

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