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Effects of CeCl_3 and LaCl_3 on callus and root induction and the physical response of tobacco tissue culture

Journal of Rare Earths 2018年第4期36卷 440-448页

作者： Guicheng Song Pingping Zhang Gaoling Shi Huadun Wang Hongxiang Ma Provincial Key Laboratory of Agrobiology Institute of Food Crops Jiangsu Academy of Agricultural Sciences

La3＋ and Ce3＋ have positive effects on plant growth and production. Although it is well known that rare earth elements promote cell growth. The biological effects of La^（3＋） and Ce^（3＋） on callus, shoot and root induction in tobacco are still unclear. The relationships among callus induction, rooting, enzyme activities and stomatal characteristics in tobacco are unknown. The objectives of this study were to identify the relationships between the induction of calluses, shoots, roots, stomata and enzyme activities. The induction percentages of calluses, buds, roots were recorded at 5,10,15, 20 and 25 days after La^（3＋） and Ce^（3＋） treatments. Peroxidase isoenzyme activity was determined by electrophoresis. The characteristics of the stomata were observed under an optical microscope. Our results show that low concentrations of Ce^（3＋）（〈15 mg/L） result in increases in the induction percentages of calluses,buds and roots, but La^（3＋）（〉5 mg/L） inhibits the induction of calluses, buds and roots. There are more peroxidase isoenzyme bands in Ce^（3＋） treatments than in La^（3＋） treatments. This is consistent with the induction percentages of calluses,buds and roots in Ce^（3＋） and La^（3＋） treatments. High enzyme activities may promote the induction of calluses, buds and roots. The stomata area and stomata number of leaves are significantly different between La^（3＋） treatments and Ce^（3＋） treatments. La^（3＋） improves the stomata area and number. Based on these results, we speculate that La^（3＋） may promote the development of the photosynthetic system. Ce^（3＋）may promote tobacco growth and rooting by improving enzyme activities.

关键词： Cerium chloride Lanthanum chloride Callus induction Tissue culture POD expression Stomata

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A systematic review of highland barley:Ingredients,health functions and applications

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Grain & Oil Science and Technology 2022年第1期5卷 35-43页

作者： Yiming Lyu Sen Ma Jingke Liu Xiaoxi Wang College of food Science and Engineering Henan University of TechnologyZhengzhou 450001China institute of Millet crops Hebei Academy of Agriculture and Forestry SciencesNational FoxtailMillet Improvement CenterMinor Cereal Crops Laboratory of Hebei ProvinceShijiazhuang 050035China

Due to its high nutritional and dietotherapy values,highland barley has attracted the attention and favor of people all over the world in recent years.It has been demonstrated that the nutritional components of highland barley are comprehensive and unique,with the characteristics of high protein,high fiber,high vitamin,lowfat,low sugar and variety of bioactive components.As the most important component in highland barley,highland barley starch not only has low digestibility,but also has good freeze-thaw stability,high solubility,good emulsion stability and superior film forming performance,which makes it have great application value in food,medicine and industrial production.The content of highland barley protein,which is rich in 18 kinds of amino acids,is higher than that of most grains,and its derivatives play an important role in medical treatment.Unfortunately,highland barley protein cannot form gluten network structure,which limits the application in daily staple food to a certain extent.Highland barley also contains a large amount of dietary fiber,especiallyβ-glucan.Long-term consumption could significantly reduce the incidence of chronic diseases and metabolic syndromes such as heart disease and diabetes.However,the research on the application of highland barley is still in the laboratory stage,which failed to achieve large-scale application in the actual production.The value of highland barley has not been brought into full play,which leads to the waste of its resources and the reduction of its added value.This paper reviewed themacronutrients,health functions and applications of highland barley,aiming to provide some reference for the development of highland barley in food and health industry.

关键词： Highland barley Macronutrients Physicochemical property Application

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Rational Design of Grain Size to Improve Rice Yield and Quality

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Rice science 2023年第1期30卷 1-5页

作者： TAO Yajun WANG Jun XU Yang WANG Fangquan LI Wenqi JIANG Yanjie CHEN Zhihui FAN Fangjun ZHU Jianping LI Xia YANG Jie institute of food crops Jiangsu Academy of Agricultural Sciences/Nanjing Branch of Chinese National Center for Rice ImprovementNanjing 210014China Jiangsu Co-Innovation Center for Modern Production Technology of Grain crops Yangzhou UniversityYangzhou 225009China

Grain size, determined by grain length, grain width and grain thickness, is associated with grain yield and quality. Many genes controlling grain size were cloned and their related regulatory mechanisms were clearly clarified. However, whether these genes can be directly introduced into japonica rice for grain size improvement is unknown.

关键词： Yield Rational size

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Research Advances in Functional Constituents of Chickpea

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Asian Agricultural Research 2019年第4期11卷 59-61页

作者： Haitian YU Aiqing ZHENG Meiyuan LU Feng YANG Chaoqin HU Xin YANG Yuhua HE Liping WANG institute of food crops Yunnan Academy of Agricultural Sciences

Based on the generalization and summary of the research on the functional constituents of chickpea at home and abroad in recent years,the research advances in some rich functional constituents including isoflavones,proteins and peptides,carbohydrates,saponins and trace elements in chickpeas was reviewed in this paper. It provides a basis for the research,development and utilization of the functional constituents of chickpea in the future.

关键词： Chickpea Functional constituents Advances

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Induction of Tolerance to Fusarium Wilt and Defense-Related Mechanisms in the Plantlets of Susceptible Berangan Banana Pre-Inoculated with Pseudomonas sp.(UPMP3) and Burkholderia sp.(UPMB3)

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Agricultural Sciences in China 2010年第8期9卷 1140-1149页

作者： Elya Masya Mohd Fishal Sariah Meon Wong Mui Yun Laboratory of food crops and Floriculture Institute of Tropical AgricultureUniversiti Putra Malaysia

This study is aimed at assessing the ability of two endophytic bacteria originally isolated from healthy oil palm roots, Pseudomonas sp. （UPMP3） and Burkholderia sp. （UPMB3） to induce resistance in susceptible Berangan banana against Fusarium oxysporum race 4 （FocR4）. Increased accumulation of resistance-related enzymes such as peroxidase （PO）, phenylalanine ammonia lyase （PAL）, lignithioglycolic acid （LTGA）, and pathogenesis-related （PR） proteins （ehitinase and β-1,3-glucanase） has been observed in plantlets treated with endophytic bacteria UPMP3 and UPMB3 singly or as mixture under glasshouse conditions. Pre-inoculation of banana plantlets with UPMP3 showed a significant reduction in Fusarium wilt incidence 72 d after challenged inoculation with FocR4. UPMB3 was less effective in suppressing Fusarium wilt compared to UPMP3, whereas, the mixture of both endophytes showed an intermediate effect. Based on these results, it is concluded that UPMP3 could be a promising biological control agent that can trigger resistance against Fusarium wilt in susceptible Berangan banana.

关键词： banana Pseudomonas sp. Burkholderia sp. induce resistance Fusarium wilt

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Transcriptome analysis and resistance identification of highgeneration Bar and BPH9 transgenic rice

Transcriptome analysis and resistance identification of high...

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第二十届中国作物学会学术年会

作者： SanheLi ChangyanLi BianWu YanWu WenjunZha LeiZhou Aiqing You Hubei Key Laboratory of food Crop Germplasm and Genetic Improvement Food Crops Institute Hubei Academy of Agricultural Sciences

Insect pests and weeds are the two major biotic factors affecting crop yield in the modern agricultural system. In this study, a brown planthopper(BPH) resistance gene(BPH9) and glufosinate tolerance gene(bar) were stacked into a single T-DNA cassette and transformed into an indica rice(Oryza sativa L.) line Guangzhan 63-4S. The present study employed a gene stacking process that combines more than one gene/trait into an individual transgenic plant to meet the increasing cropping demands under complex conditions. The transgenic rice H23 coexpressing bar and BPH9 genes demonstrated both glufosinate tolerance and BPH resistance. We utilized transcriptome data to reveal the mechanism of BPH9 mediated brown planthopper resistance, and to analyze the impact of exogenous transgenic fragments on upstream and downstream genes at insertion sites. Evaluation of insect resistance and glufosinate tolerance confirmed H23 as an excellent double-resistant transgenic rice. These findings indicate that H23 can satisfy insect management and weed control in the modern rice agricultural system. However, a deregulation study will help for prospective commercial planting.

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Advances in the Identification of Genetically Modified Rice with Real-time PCR and Multiplex PCR

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Agricultural Biotechnology 2017年第3期6卷 23-25,29页

作者： Juan QIU institute of food crops Hubei Academy of Agricultural Sciences

In recent years, food security and safety have attracted increasing attention due to the worldwide research and development of genetically modified （GM） rice, and the controversy over the commercialization of GM rice. And the identification of GM rice is of great significance. Therefore, in the present study, the po- tential problems in the identification of GM rice with PCR were analyzed both at a technical level and from a theoretical perspective. In addition, PCR detection on the transgenic elements： promoter, terminator, internal reference gene and target gene was discussed, respectively. The possible solutions were proposed based on the principles of plant virology and genetic engineering.

关键词： Genetically modified （GM） rice Qualitative detection Promoter Terminator Bt gene Multiplex PCR Real-time PCR

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Photosynthetic properties of the mid-vein and leaf lamina of field-grown, high-yield hybrid rice during senescence

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Journal of Integrative Agriculture 2022年第7期21卷 1913-1926页

作者： GAO Zhi-ping XU Min-li ZHANG Hai-zi Lü Chuan-gen CHEN Guo-xiang College of Life Sciences Nanjing Normal UniversityNanjing 210023P.R.China Institute of Food and Crops Jiangsu Academy of Agricultural SciencesNanjing 210014P.R.China

Previous studies with rice(Oryza sativa L.) have shown that the different components of the photosynthetic apparatus are not uniformly synthesized or degraded during senescence. However, while most of those studies have focused on the leaf lamina, few have addressed senescence-associated chloroplast function or leaf physiology. Here, we investigated the photosynthetic properties of the mid-vein and leaf lamina in a high-yield hybrid rice cultivar(Liangyoupei 9, LYP9) during the senescence stage. Assimilation and transpiration decreased more slowly in the mid-vein than in the lamina during senescence, suggesting more sustained photosynthesis in the mid-vein, as well as stronger heat dissipation. Two-dimensional gel electrophoresis suggested that photosynthesis and energy metabolism were less affected by senescence in the mid-vein than in the leaf lamina. During late senescence, the excess energy dissipation in the midvein through the xanthophyll cycle had a higher active photosynthetic capacity than in the leaf lamina, and we inferred that the mid-vein and leaf lamina of LYP9 rice differed in terms of their maturation. Taken together, these results provide new insights into the underlying mechanisms of senescence of the rice mid-vein and associated physiology.

关键词： mid-vein rice photosynthetic properties senescence

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Seed Length Controlled by Same Locus in Four Different AA Genome Species of Genus Oryza

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Rice science 2014年第1期21卷 20-28页

作者： ZHANG Yu LI Jing ZHOU Jia-wu XU Peng DENG Xian-neng YANG Fei DENG Wei HU Feng-yi TAO Da-yun Institute of Food and Crops Research Yunnan Academy of Agricultural Sciences Life-Sciences School Yunnan University

To broaden the genetic basis and overcome the yield plateau in Asian cultivated rice, the exploitation and utilization of favorable alleles from rice species with the AA genome has become important and urgent in modern breeding programs. Four different interspecific populations were used to detect quantitative trait locus （QTL） for seed length, including a BC4F2 population derived from Oryza glumaepatula crossed with Dianjingyou 1 （a japonica cultivar）, a BC4F2 population derived from O. nivara crossed with Dianjingyou 1, a BCzF1 population derived from a cross between O. Iongistaminata and RD23 （an indica cultivar）, and a BCsF1 population derived from a cross between O. glaberrima and Dianjingyou 1. The QTLs for seed length in four different populations were termed as SL-3a, SL-3b, SL-3c and SL-3d, respectively. They had good collinearity and accounted for 49% to 60% of the phenotypic variations. Sequencing data indicated that four QTLs were different alleles of GS3 which were responsible for the seed length variation between O. sativa and its four AA genome relatives. These results will be valuable for confirming the evolution of GS3 and also be helpful for rice breeding.

关键词： seed length O. glumaepatula O. nivara O. Iongistaminata O. glaberrima quantitative trait locus

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Effect of Seed Bacterization on Plant Growth Response and Induction of Disease Resistance in Chilli

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Agricultural Sciences in China 2009年第8期8卷 963-971页

作者： Yasmeen Siddiqui Sariah Meon Laboratory of food crops and Floriculture Institute of Tropical AgricultureUniversiti Putra Malaysia (UPM)

This study aimed to examine the induction of disease resistance, and growth response in chilli plants elicited by plant growth promoting endophytic bacteria [Pseudomonas aeruginosa （UPMP3）, Burkholderia cepacia （UPMB3）, and Serratia marcescens （UPMS3）]. Seed bacterization with UPMP3 and UPMB3 significantly increased peroxidase （PO）, polyphenol oxidase （PPO）, and phenylalanine ammonia-lyase （PAL） activities. This increase corresponded to greater reduction in pre-and post-emergence damping-off caused by Sclerotium rolfsii. UPMS3 alone or as mixture with UPMP3 and UPMB3 did not show any significant reduction in disease incidence. However, all the isolates tested did not inhibit the seed germination and seedling establishment in chilli.

关键词： endophytic bacteria induced resistance PO PPO PAL Sclerotium rolfsii

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