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Journal of Zhejiang University-science B(Biomedicine & Biotechnology) 2010年第10期11卷 792-800页

作者： Jun-mei WANG Jian-ming YANG Jing-huan ZHU Qiao-jun JIA Yue-zhi TAO institute of crop and nuclear technology utilization Zhejiang Academy of Agricultural Sciences Hangzhou 310021 China

The genetic diversity and relationship among 40 elite barley varieties were analyzed based on simple sequence repeat （SSR） genotyping data. The amplified fragments from SSR primers were highly polymorphic in the badey accessions investigated. A total of 85 alleles were detected at 35 SSR loci, and allelic variations existed at 29 SSR loci. The allele number per locus ranged from 1 to 5 with an average of 2.4 alleles per locus detected from the 40 badey accessions. A cluster analysis based on the genetic similarity coefficients was conducted and the 40 varieties were classified into two groups. Seven malting barley varieties from China fell into the same subgroup. It was found that the genetic diversity within the Chinese malting barley varieties was narrower than that in other barley germplasm sources, suggesting the importance and feasibility of introducing elite genotypes from different origins for malting barley breeding in China.

关键词： Barley (Hordeum vu/gare L.) Genetic similarity Simple sequence repeat (SSR) marker Cluster analysis Genetic diversity

Simulation Study of Quantitative X-Ray Fluorescence Analysis of Ore Slurry Using Partial Least-Squares Regression

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Plasma science and technology 2012年第5期14卷 427-430页

作者：林才寿毛莉黄宁安竹 Key Laboratory of Radiation Physics and technology of Ministry of Education Institute of Nuclear Science and TechnologySichuan University institute of Multipurpose utilization of Mineral Resources

X-ray fluorescence （XRF） in combination with partial least-squares （PLS） regression was employed to analyze the ore slurry grade. Using the Monte Carlo simulation code PENELOPE, X-ray fluorescence spectra of ore samples were obtained. Good accuracy was achieved when this method was used to analyze elements with concentrations of several percent or above. It was demonstrated that the more the number of X-ray fluorescence spectra used to calibrate, the better the obtained accuracy. In this method detector resolution was found to have little or no effect on the results of quantitative analysis. The effect of the concentration of water was investigated as well, and it was found to have little influence on the results.

关键词： X-ray fluorescence PLS ore slurry grade Monte Carlo simulation

Cytological Effects of Space Environment on Different Genotype of Rice

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Journal of Beijing institute of technology 2007年第2期16卷 220-225页

作者：魏力军钱宇杨谦徐建龙王俊敏孙野青 Department of Life science and Engineering Harbin Institute of Technology National Key Facility for crop Gene Resources & Genetic Improvement institute of crop and nuclear technology utilization Zhejiang Academy of Agricultural Sciences institute of Environmental Systems Biology Dalian Maritime University

For exploring the biological effect of space environment on different genotype of rice seeds, the cytological effects of M1 generation after space flight were studied. Twelve different genotypes of rice seeds which belong to different climate ecotype （early, medium and late） of indica and japonica were onboard ＂Shenzhou 4＂ spaceship for 162 h. After recovered the total number of mitosis cells and chromosomal aberration were observed. In all the lines the mitotic index （MIs） of space flight are much higher than control, which indicates the stimulate effect of space environment. The cell rate of chromosomal aberration （CRCA） of space flight is also much higher than control, but varies from line to line. It indicates that biological effect of space environment on rice seed dependents not only on flight duration but also on rice genotype. The radiosensitivities of different lines were also discussed according to CRCAs.

关键词： rice space flight cytological effects

OsVP1 activates Sdr4 expression to control rice seed dormancy via the ABA signaling pathway

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The crop Journal 2021年第1期9卷 68-78页

作者： Wenqiang Chen Wei Wang Yusong Lyu Yawen Wu Pingliang Huang Shikai Hu Xiangjin Wei Guiai Jiao Zhonghua Sheng Shaoqing Tang Gaoneng Shao Ju Luo State Key Laboratory of Rice Biology China National Rice Research InstituteHangzhou 310006ZhejiangChina institute of crop science and nuclear technology utilization Zhejiang Academy of Agricultural SciencesHangzhou 310021ZhejiangChina

Pre-harvest sprouting(PHS)is a disadvantageous trait in cereal production worldwide,causing large economic losses each *** regulation mechanism is still *** generated the Oryza sativa Viviparous1(OsVP1)mutant using gene editing technique,which shows increased PHS compared with that of the wild type ***1 is localized mainly in the nucleus and expressed in various tissues and *** of Seed dormancy 4(Sdr4),a key gene controlling PHS,was sharply reduced in OsVP1 ***1 bound to the specific motif CACCTG in the promoter of Sdr4 and activated its expression in rice *** of Sdr4 reduced the high seed germination rate of OsVP1 mutant cr-osvp1-1,showing that Sdr4 acts as a downstream target of *** OsVP1 and Sdr4 loss-of-function mutants were insensitive to exogenous ABA and employed the ABA signaling pathway in regulating seed *** findings shed light on the control of seed dormancy aimed at preventing PHS in rice.

关键词： Rice Pre-harvest sprouting Seed dormancy OsVP1 Sdr4 ABA

Quantitative Trait Locus Analysis for Rice Yield Traits under Two Nitrogen Levels

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Rice science 2015年第3期22卷 108-115页

作者： FENG Yue ZHAI Rong-rong LIN Ze-chuan CAO Li-yong WEI Xing-hua CHENG Shi-hua Chinese National Center for Rice Improvement / State Key Laboratory of Rice Biology China National Rice Research Institute institute of crop and nuclear technology utilization Zhejiang Academy of Agricultural Sciences

A recombinant inbred line population derived from a super hybrid rice Xieyou 9308（Xieqingzao B/Zhonghui 9308） and its genetic linkage map were used to detect quantitative trait loci（QTLs） for rice yield traits under the low and normal nitrogen（N） levels. A total of 52 QTLs for yield traits distributed in 27 regions on 9 chromosomes were detected, with each QTL explaining 4.93%–26.73% of the phenotypic variation. Eleven QTLs were simultaneously detected under the two levels, and 30 different QTLs were detected under the two N levels, thereby suggesting that the genetic bases controlling rice growth under the low and normal N levels were different. QTLs for number of panicles per plant, number of spikelets per panicle, number of filled grains per panicle, and grain density per panicle under the two N levels were detected in the RM135–RM168 interval on chromosome 3. QTLs for number of spikelets per panicle and number of filled grains per panicle under the two N levels, as well as number of panicles per plant and grain density per panicle, under the low N level, were detected in the RM5556–RM310 interval on chromosome 8. The above described QTLs shared similar regions with previously reported QTLs for rice N recycling.

关键词： nitrogen quantitative trait locus rice yield trait

Anatomical and Chemical Alterations but not Photosynthetic Dynamics and Apoplastic Transport Changes are Involved in the Brittleness Culm Mutation of Rice

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Journal of Integrative Plant Biology 2008年第12期50卷 1508-1517页

作者： Zhuang-Qin Duan Jun-Min Wang Lei Bai Zhi-Guang Zhao Kun-Ming Chen institute of crop sciences College of Agriculture and Biotechnology Zhejiang University Hangzhou 310029 China College of Life sciences Lanzhou University Lanzhou 730000 China institute of crop and nuclear technology utilization Zhejiang Academy of Agricultural Sciences Hangzhou 310029 China

Brittleness culm is an important agronomic trait that has a potential usefulness in agricultural activity as animal forage although the developmental mechanism is not clear yet. In the present study, the anatomical and chemical characteristics as well as some ecophysiological features in the brittleness culm mutation of rice （Oryza sativa L.） were investigated. Compared with the wild type （WT）, the brittleness culm mutant （bcm） exhibited higher culm vascular bundle distance and lower culm wall thickness, leaf interveinal distance and leaf thickness. Ratio of bundle sheath cell/whole bundle and areas of whole vascular bundles and bundle sheath of leaves were reduced while ratios of xylem and phloem to whole bundles were elevated in bcm. The Fourier transform infrared （FTIR） microspectroscopy analysis and further histochemical and physiological measurements revealed that the different contents and depositions of cell wall components such as pectins, lignin, suberin and cellulose all participated in the mutation of brittleness. However, the mutant presented no significant changes in leaf photosynthetic dynamics and apoplastic transport ability. These results strongly indicate that the alterations in anatomical and chemical characteristics, rather than changes in major ecophysiological features such as photosynthesis and apoplastic transport were involved in the brittleness mutation of rice.

关键词： anatomical and chemical characteristics apoplastic transport brittleness culm mutant photosynthetic dynamic rice.

Characterization of a PDR type ABC transporter gene from wheat (Triticum aestivum L.)

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Chinese science Bulletin 2009年第18期54卷 3249-3257页

作者： SHANG Yi XIAO Jin MA LuLin WANG HaiYan QI ZengJun CHEN PeiDu LIU DaJun WANG XiuE State Key Laboratory of crop Genetics and Germplasm Enhancement Cytogenetics Institute Nanjing Agricultural UniversityNanjing 210095 China institute of crop Research and nuclear Technique utilization Zhejiang Academy of Agricultural Science Hangzhou 310021 China

DON, as a virulence factor, plays an important role in the infection of Fusarium graminearum in wheat. The infection ability of F. graminearum depends on its capacity of producing DON. The production of DON by F. graminearum is significantly decreased in the wheat varieties with scab resistance. In this study, GeneChip analysis indicated that an EST encoding an ATP-binding cassette (ABC) transporter was up-regulated by 45 times in a wheat landrace Wangshuibai, which is resistant to DON accumulation. A pair of EST-derived primers were designed based on the EST sequence, and a clone was then isolated from a wheat genomic DNA TAC library. The TAC clone was sequenced using chromosome walking and gene prediction was conducted using Softberry. A cDNA clone of this gene was subsequently isolated from Wangshuibai induced by DON using gene-specific primers designed according to the untranslated sequence of the gene. The genome size of the gene is 7377 bp, consisting of 19 exons with coding sequences of 4308 bp. It encodes a protein with 1435 amino acid residues and the calculated molecular weight is about 161 kD. BLAST analysis indicated that the gene may belong to pleiotropic drug resistance (PDR) sub-family, and hence designated as TaPDR1 (Triticum aestivum pleiotropic drug resistance). TaPDR1 was located on chromosome 5A of wheat using nullisomic-tetrasomic lines of Chinese Spring. TaPDR1 was up-regulated by induction of both DON and F. graminearum. Expression patterns of TaPDR1 were different in wild-type Wangshuibai and the fast-neutron induced Wangshuibai mutant lacking FHB1, a major QTL of FHB resistance and DON resistance in chromosome arm 3BS. These results suggested that TaPDR1 might be a candidate gene responsible for DON ac-cumulation resistance. The expression profile showed that TaPDR1 expression was neither induced by hormones typically involved in biotic stress, such as JA and SA, nor by abiotic stresses, such as heat, cold, wounding and NaCl. However, TaPDR1

关键词：小麦多样性 PDR ABC

Characterization and molecular cloning of a serine hydroxymethyltransferase 1(OsSHM_1) in rice

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Journal of Integrative Plant Biology 2015年第9期57卷 745-756页

作者： Dekai Wang Heqin Liu Sujuan Li Guowei Zhai Jianfeng Shao Yuezhi Tao institute of crop and nuclear technology utilization Zhejiang Academy of Agricultural Sciences China State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control Zhejiang Academy of Agricultural Sciences

Serine hydroxymethyltransferase (SHMT) is important for one carbon metabolism and photorespiration in higher plants for its participation in plant growth and development, and resistance to biotic and abiotic stresses. A rice serine hydroxymethyltransferase gene, OsSHM1, an ortholog of Arabidopsis SHM1, was isolated using map-based cloning. The osshm1 mutant had chlorotic lesions and a considerably smaller, lethal phenotype under natural ambient CO2 concentrations, but could be restored to wild type with normal growth under elevated CO2 levels (0.5% CO2), showing a typical photorespiratory phenotype. The data from antioxidant enzymes activity measurement suggested that osshm1 was subjected to significant oxidative stress. Also, OsSHM1 was expressed in all organs tested (root, culm, leaf, and young panicle) but predominantly in leaves. OsSHM1 protein is localized to the mitochondria. Our study suggested that molecular function of the OsSHM1 gene is conserved in rice and Arabidopsis.

关键词： gene molecular cloning reactive oxygen species rice serine hydroxymethyltransferase

OspTAC2 encodes a pentatricopeptide repeat protein and regulates rice chloroplast development

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Journal of Genetics and Genomics 2016年第10期43卷 601-608页

作者： Dekai Wang Heqin Liu Guowei Zhai Liangsheng Wang Jianfeng Shao Yuezhi Tao institute of crop and nuclear technology utilization Zhejiang Academy of Agricultural Sciences State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control Zhejiang Academy of Agricultural Sciences Boyce Thompson institute for Plant Research

Functional chloroplast generation depends on the precise coordination of gene expression between the plastid and the nucleus and is essential for plant growth and development. In this study, a rice（Oryza sativa） mutant that exhibited albino and seedling-lethal phenotypes was isolated from a60Co-irradiated rice population. The mutant gene was identified as an ortholog of the Arabidopsis plastid transcriptionally active chromosome protein 2（p TAC2） gene, and the mutant strain was designated osptac2. Sequence and transcription analyses showed that Osp TAC2 encodes a putative chloroplast protein consisting of 10 pentratricopeptide repeat（PPR） domains and a C-terminal small Mut S-related（SMR） domain. Cytological observations via microscopy showed that the Osp TAC2-green fluorescent fusion protein is localized in the chloroplasts. Transmission electron microscopy revealed that the chloroplast of the osptac2 mutant lacks an organized thylakoid membrane. The transcript levels of all investigated PEP（plastid-encoded RNA polymerase）-dependent genes were dramatically reduced in the osptac2 mutant, whereas the transcript levels of NEP（nuclear-encoded polymerase）-dependent genes were increased. These results suggest that Osp TAC2 plays a critical role in chloroplast development and indicate that the molecular function of the Osp TAC2 gene is conserved in rice and Arabidopsis.

关键词： Oryza sativa Pentatricopeptide repeat protein Chloroplast development

Overexpression of a maize SNF-related protein kinase gene, ZmSnRK2.11, reduces salt and drought tolerance in Arabidopsis

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Journal of Integrative Agriculture 2015年第7期14卷 1229-1241页

作者： ZHANG Fan CHEN Xun-ji WANG Jian-hua ZHENG Jun College of Agriculture and Biotechnology China Agricultural University institute of crop science Chinese Academy of Agricultural Sciences institute of nuclear technology and Biotechnology Xinjiang Academy of Agricultural Sciences

Sucrose non-fermenting-1 related protein kinase 2 （SnRK2） is a unique family of protein kinases associated with abiotic stress signal transduction in plants. In this study, a maize SnRK2 gene ZmSnRK2.11 was cloned and characterized. The results showed that ZmSnRK2.11 is up-regulated by high-salinity and dehydration treatment, and it is expressed mainly in maize mature leaf. Atransient expression assay using onion epidermal cells revealed that ZmSnRK2.11-GFP fusion proteins are localized to both the nucleus and cytoplasm. Overexpressing-ZmSnRK2.11 in Arabidopsis resulted in salt and drought sensitivity phenotypes that exhibited an increased rate of water loss, reduced relative water content, delayed stoma closure, accumulated less free proline content and increased malondialdehyde （MDA） content relative to the phenotypes observed in wild-type （WT） control. Furthermore, overexpression of ZmSnRK2.11 up-regulated the expression of the genes ABI1 and ABI2 and decreased the expression of DREB2A and P5CSI. Taken together, our results suggest that ZmSnRK2.11 is a possible negative regulator involved in the salt and drought stress signal transduction pathways in plants.

关键词： maize salt and drought stresses ZmSnRK2.11 SnRK2