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Journal of Genetics and Genomics 2008年第10期35卷 625-629页

作者： Amir Mohammad Naji Mohammad Moghaddam Mohammad Reza Ghaffari Hashem Pour Irandoost Laleh Karimi Farsad Seyed Mostafa Pirseyedi Seyed Abolghasem Mohammadi Behzad Ghareyazie Mohsen Mardi Department of Agronomy and crop breeding Shahed University of Tehran Department of Agronomy and Plant breeding University of Tabriz Department of Genomics Agricultural Biotechnology Research Institute of Iran

A few EST-derived STS markers localized on ***-3BS, a major QTL for resistance to Fusarium head blight （FHB） in wheat, have been previously identified in the ＇Sumai 3＇/＇Stoa＇ population. In this study, we used a ＇Wangshuibai＇（resistant）/＇Seri82＇（susceptible） derived population, linkage group, QTL, and quantitative gene expression analysis to assess the genetic background dependence and stability of the EST-derived STS markers for use in marker aided selection to improve FHB resistance in wheat. Based on our results, a QTL in the map interval of Xsts3B-138_1-Xgwrn493 on chromosome 3BS was detected for FHB resistance, which accounted for up to 16% of the phenotypic variation. BLASTN analysis indicated that Xsts3B-138_1 sequence had significant similarity with the resistance gene analogue. Real-time quantitative PCR showed that the relative expression of Xsts3B-138_1 in ＇Wangshuibai＇ at 96 h after inoculation was 2.6 times higher than ＇Seri82＇. Our results underlined that EST-derived STS3B-138 markers could be predominantly used in marker aided selection to improve FHB resistance in wheat.

关键词： EST-derived STS markers Qfhs.ndsu-3BS Fusarium head blight wheat

GSK3 regulates VRN1 to control flowering time in wheat

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Journal of Integrative Plant Biology 2023年第7期65卷 1605-1608页

作者： Guoqing Cui Danping Li Lichao Zhang Chuan Xia Xiuying Kong Xu Liu State Key Laboratory of crop Gene Resources and breeding Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China

The precise control of flowering time is important for the regional adaptability and productivity of many *** categories of genes related to flowering have been isolated and characterized functionally in wheat(Triticum aestivum) in response to vernalization to adjust flowering initiation. Before vernalization, the inhibitory histone modification H3K27me3 is enriched in the promoter and the first intron of the vernalization gene VRN1.

关键词： aestivum precise flowering

Distribution of Eight Blast-resistant Genes in Parents of Three-line Japonica Hybrid Rice

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Plant Diseases and Pests 2018年第2期9卷 1-5,15页

作者： Chu Huangwei Cheng Can Niu Fu’an Zhou Jihua Tu Rongjian Luo Zhongyong Wang Xinqi Cao Liming crop breeding and cultivation Research institute Shanghai Academy of Agricultural Sciences

The distribution of rice blast-resistant genes Pi2, Pi5, Pi9, Pi54, Pia, Pib, Pit and Pita in 36 restoring lines and 42 sterile lines were detected using functional molecular markers. The results indicated that those eight blast-resistant genes distributed in the 78 tested lines at various frequencies. Six rice blast-resistant genes were detected in the sterile line Shen 11 A, containing the most rice blast-resistant genes among the 78 lines, whereas no rice blast-resistant gene was detected in the lines 8527 A, Xu1 A and 016 A. The genetic distance-based clustering analysis showed that those 78 lines could be divided into five groups.

关键词： Hybrid japonica rice Rice blast Resistance gene

Identification of novel QTL associated with soybean isoflavone content

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The crop Journal 2018年第3期6卷 244-252页

作者： Ruili Pei Jingying Zhang Ling Tian Shengrui Zhang Fenxia Han Shurong Yan Lianzheng Wang Bin Li Junming Sun The National Engineering Laboratory for crop Molecular breeding MOA Key Laboratory of Soybean Biology (Beijing)Institute of Crop SciencesChinese Academy of Agricultural Sciences

Soybean isoflavones are essential secondary metabolites synthesized in the phenylpropanoid pathway and benefit human health. In the present study, highresolution QTL mapping for isoflavone components was performed using specific-locus amplified fragment sequencing(SLAF-seq) with a recombinant inbred line(RIL) population(F5:7) derived from a cross between two cultivated soybean varieties, Luheidou 2(LHD2) and Nanhuizao(NHZ). Using a high-density genetic map comprising 3541 SLAF markers and the isoflavone contents of soybean seeds in the 200 lines in four environments, 24 stable QTL were identified for isoflavone components, explaining 4.2%–21.2% of phenotypic *** these QTL, four novel stable QTL(qG8, qMD19, qMG18, and qTIF19) were identified for genistin, malonyldaidzin, malonylgenistin, and total isoflavones, respectively. Gene annotation revealed three genes involved in isoflavone biosynthesis(Gm4CL, GmIFR, and GmCHR) and 13 MYB-like genes within genomic regions corresponding to stable QTL intervals, suggesting candidate genes underlying these loci. Nine epistatic QTL were identified for isoflavone components, explaining 4.7%–15.6% of phenotypic variation. These results will facilitate understanding the genetic basis of isoflavone accumulation in soybean seeds. The stable QTL and tightly linked SLAF markers may be used for markerassisted selection in soybean breeding programs.

关键词： Soybean(Glycine max L.Merrill) QTL mapping Isoflavones Specific-locus amplified fragment sequencing(SLAF-seq)

Determination of Amylose Content and Its Relationship with RVA Profile Within Genetically Similar Cultivars of Rice(Oryza sativa ***)

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Agricultural Sciences in China 2010年第8期9卷 1101-1107页

作者： WANG Xin-qi YIN Lin-qing SHEN Ge-zhi XU Li LIU Qiao-quan crop breeding and cultivation Research institute Shanghai Academy of Agricultural Sciences Shanghai 201106 P..R. China Key Laboratory of Plant Functional Genomics Ministry of Education/Yangzhou Agricultural College Yangzhou 225009 P.R.China

Anther culture pure lines with antisense Wx gene were generated by Agrobacterium tumefaciens-mediated co-transformation followed by anther culture in transgenic rice （Oryza sativa L. ssp. japonica）. The antisense Wx transgenic pure lines were used to analyze the differences and the relationship between RVA eigenvalues among the lines with different amylose contents. 77 pure lines of anther culture having antisense Wx gene were studied for the amylose content of its offspring cultivar, Wuyunjing 7. It was found that levels ofamylose content were similar to the control in 19 lines （16.0%）; 1-5% lower than control in 50 lines, of which, within 11.0-13.9% in 40 lines; and 3.1-4.0% in 8 lines. The effect of the amylose content on RVA eigenvalue was analyzed through the RVA profile graphic comparison and test of significance for RVA eigenvalues. The analysis of RVA profiles with different amylose contents indicated that there were two kinds of RVA profiles within genetically similar cultivars. The lines with 3.1-4.0% of amylose content were distinctly different from other lines and conventional glutinous rice. The comparison of the similarity curve of RVA profile of the lines with different amylose contents showed that the lines with lower amylose content had a distinguished RVA profile with the curve increasing gradually, but not exceeding the first apex. The test of significance for eigenvalues of the RVA profile in the lines with different amylose contents indicated that there were five eigenvalues remarkably different among the lines with 3.1-4.0% of amylose content compared with other two groups. Further, there were three eigenvalues remarkably different among the lines with 11.0-13.5% of amylose content compared with control group. An optimized mathematical model, which characterizes the relationships between the amylose content and RVA eigenvalue, has been established in this study. From this model, it was clearly indicated that the parameters PeT and SBV were more

关键词： antisense Wx gene amylose content RVA profile rice

Sap flow characteristics of three afforestation species during the wet and dry seasons in a dry-hot valley in Southwest China

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Journal of Forestry Research 2017年第1期28卷 51-62页

作者： Xiaofei Wang Jianfeng Liu Yongyu Sun Kun Li Chunhua Zhang Research institute of Resources Insects Chinese Academy of Forestry Key Laboratory of Tree breeding and cultivation of State Forestry Administration Research Institute of ForestryChinese Academy of Forestry

Assessing and using tree species （exotic or native） with superior tolerance to environmental stresses （such as drought and high temperature） play an important role in afforestation practices. In the present study, stem sap flow characteristics and responses to ambient meteo- rological factors of three tree species, Albizzia kalkora （native）, Azadirachta indica （exotic）, and Acacia auriculaeformis （exotic）, in a dry-hot valley （Yuanmou, Yunnan Province, China） were investigated using thermal dissipation probes. The diurnal dynamics of sap flow in three studied species displayed an obvious circadian rhythm during the wet and dry seasons, with the exception of A. indica during the dry season. The sap flow velocity （SFV） in A. kalkora and A. auriculaeformis was significantly positively correlated with photosynthetically active radiation （PAR）, air temperature, vapour pressure deficit （VPD） and wind speed, but negatively correlated with atmospheric relative humidity over the two seasons. The cross-corre- lation analysis also revealed that the SFV of the three species was significantly correlated with PAR and VPD （P 〈 0.001）. Additionally, stem sap flow lagged behind PAR but ahead of VPD, and the diurnal sap flow was more dependent on PAR than on VPD. However, we found that the dominant climatic factor influencing the stem sap flow differed between daytime and nighttime. PAR was more influential than other meteorological factors during the daytime, while VPD or other factors were more influential overnight. When the nighttime refilling ability of the three tree species was compared, our results suggest that A. indica has higher drought resistance and better for afforestation of the studied region.

关键词： Sap flow Thermal dissipation probes Meteorological factors Refill Dry-hot valley

Transcriptome-based identification and expression analysis of the glutathione S-transferase(GST)family in tree peony reveals a likely role in anthocyanin transport

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Horticultural Plant Journal 2022年第6期8卷 787-802页

作者： Lulu Han Hongzhu Zou Lin Zhou Yan Wang Research institute of Forestry Key Laboratory of Tree Breeding and Cultivation of National Forestry and Grassland AdministrationChinese Academy of ForestryBeijing 100091China

For red-flowered cultivars of tree peony(Paeonia suffruticosa),anthocyanin content is a critical factor determining the different petal *** S-transferases(GSTs)are ubiquitous and multifunctional conjugating proteins that may be responsible for the transport of anthocyanin pigments from the cytoplasm to *** underlying function of the GST family in tree peony,however,remains *** this study,we systematically isolated and identified a total of 54 putative full-length Ps GST genes through a combination of bioinformatics approaches from transcriptome *** phylogenetic analyses revealed extensive differentiation in their coding sequences and divided them into 10 of the 14 known classes of plant *** phylogenetic relationships,evolutionary characteristics,protein domain,and motif organization were clearly conserved among the different phylogenetic *** results of the RNA-seq and quantitative real-time polymerase chain reaction experiments exhibited extensive variation in gene expression profiles among different developmental stages and ***,the phylogenetic relationships,expression profiles,protein interactions,weighted gene co-expression network analysis,and correlation analysis results suggested that PsGSTF3(Unigene 0064200)is a candidate participant in anthocyanin transport and the promotion of pigment accumulation,exhibiting a strong positive correlation with anthocyanin content among different tissues(r=0.908**)and an increasing rate of anthocyanin content during the flower developmental process(r=0.961*).These results furthered our understanding of the transport and accumulation functions of the GST family as well as the enhancement of tree peony breeding through molecular biology techniques.

关键词： GST Paeonia suffruticosa Functional prediction Expression analysis Anthocyanin transportation

Comparative analysis of metabolite changes in two contrasting rice genotypes in response to low-nitrogen stress

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The crop Journal 2018年第5期6卷 464-474页

作者： Xiuqin Zhao Wensheng Wang Ziyan Xie Yongming Gao Chunchao Wang Muhammed Mahbubur Rashid Mohammad Rafiqul Islam Binying Fu Zhikang Li institute of crop Sciences National Key Facility for Crop Gene Resources and Genetic Improvement Chinese Academy of Agricultural Sciences Shenzhen institute for Innovative breeding Chinese Academy of Agricultural Sciences

Identification of metabolites responsible for tolerance to low nitrogen availability(low-N)will aid in the genetic improvement of rice yield under nitrogen *** this study,a backcross introgression line(G9)and its recurrent parent Shuhui 527(SH527),which show differential responses to low-N stress,were used to identify metabolites associated with lowN tolerance in *** in metabolite contents in the leaves of G9 and SH527 at three growth stages under low-N stress were assessed by gas chromatography–mass *** metabolites,including amino acids and derivatives,were highly enriched in G9 compared with SH527 under the control condition,suggesting that the two genotypes had basal metabolite ***-N stress induced genotype-specific as well as growth stagedependent metabolite *** induced specifically in G9 that were involved in glycolysis and tricarboxylic acid metabolism were enriched at the tillering and grain filling stages,and metabolites involved in nitrogen and proline metabolism were enriched at the booting *** of pyroglutamate,glutamate,2-oxoglutarate,sorbose,glycerate-2-P,and phosphoenolpyruvic acid in G9 suggests that these metabolites could be involved in low-N stress *** results presented here provide valuable information for further elucidation of the molecular mechanisms of low-N tolerance in crops.

关键词： Rice Low-nitrogen stress Metabolite Nitrogen metabolism

Assessing bacterial communities in the rhizosphere of 8-year-old genetically modified poplar(Populus spp.)

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Journal of Forestry Research 2016年第4期27卷 939-947页

作者： Wenxu Zhu Yanguang Chu Changjun Ding Qinjun Huang Bingyu Zhang Weixi Zhang Xiaohua Su State Key Laboratory of Tree Genetics and breeding Research Institute of Forestry Chinese Academy of ForestryBeijing 100091 China Key Laboratory of Tree breeding and cultivation of State Forestry Administration Beijing 100091 China

Microbe communities in rhizosphere ecosystems are important for plant health but there is limited knowledge of them in the rhizospheres of genetically modified（GM） plants, especial for tree species. We used the amplitude sequencing method to analyze the V4 regions of the 16 S r RNA gene to identify changes in bacterial diversity and community structure in two GM lines（D520 and D521）, one non-genetically modified（nonGM） line and in uncultivated soil. After chimera filtering,468.133 sequences in the domain Bacteria remained. There were ten dominant taxonomic groups（with [1 % of all sequences） across the samples. 241 of 551 genera（representing a ratio of 97.33 %） were common to all samples.A Venn diagram showed that 1.926 operational taxonomic units（OTUs） were shared by all samples. We found a specific change, a reduction in Chloroflexi, in the microorganisms in the rhizosphere soil planted with poplars. Taken together, the results showed few statistical differences in the bacterial diversity and community structure between the GM line and non-GM line, this suggests that there was no or very limited impact of this genetic modification on the bacterial communities in the rhizosphere.

关键词： rhizosphere communities Bacteria sequencing poplar planted taxonomic representing genera microbial

A cDNA microarray analysis of the molecular control of poplar wood properties

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Journal of Forestry Research 2017年第1期28卷 71-82页

作者： Qinjun Huang Changjun Ding Weixi Zhang Bingyu Zhang Yanguang Chu Dahai Wang Xiaohua Su State Key Laboratory of Tree Genetics and breeding Research Institute of ForestryChinese Academy of ForestryBeijing 100091China Key Laboratory of Tree breeding and cultivation of State Forestry Administration Beijing 100091China

Molecular biological research into wood development and formation has been the focus in recent years, but the pace of discovery of related genes and their functions in the control of wood properties has been slow. The microarray technique--with its advantages of high throughput capacity, sensitivity, and reliability over other tools developed for investigating genes expression patterns-is capable of rapidly assaying thousands of genes. In this study, a cDNA microarray prepared from two cDNA libraries of developing poplar xylem tissues was used to assay gene expression patterns in immature xylem tissues at different heights from the main stem of Populus deltoides （15 years old）, which was confirmed to have distinct wood properties （microfibrillar angle, woody density） by X-ray. Two hundred seventy-four transcripts with differ- ential expression profles between the chips were screened out, and the individual clones were subjected to 5＇ sequencing. Using bioinformatic analysis, we identified candidate genes that may influence poplar wood properties, many of which belong to various regulatory and signal transduction gene families, such as zinc finger protein transcription factor, DNA-binding transcription factor, ethylene response factors, and so on. The results suggest that these genes may regulate enzymes involved in wood formation. Further work will be performed to clone these genes and determine how they influence poplar wood properties.

关键词： Populus deltoides cDNA microarray Expression profile Wood density Microfibfillar angle