Comparative analysis of metabolite changes in two contrasting rice genotypes in response to low-nitrogen stress

作     者：Xiuqin Zhao Wensheng Wang Ziyan Xie Yongming Gao Chunchao Wang Muhammed Mahbubur Rashid Mohammad Rafiqul Islam Binying Fu Zhikang Li 

作者机构：Institute of Crop Sciences National Key Facility for Crop Gene Resources and Genetic Improvement Chinese Academy of Agricultural Sciences Shenzhen Institute for Innovative Breeding Chinese Academy of Agricultural Sciences 

出 版 物：《The Crop Journal》 (作物学报（英文版）)

年 卷 期：2018年第6卷第5期

页      面：464-474页

核心收录：

学科分类：09[农学] 

基　　金：the National Natural Science Foundation of China (31471429) the National Key Technology R&D Program of China (2015BAD02B01-2-1,2016YFD0100904) Fundamental Research Funds for Central Non-Profit of CAAS (Y2017CG21) a Bill & Melinda Gates Foundation Project (OPP51587) the CAAS Innovative Team Award (2060302-2-18) to B.Y. Fu's team the Shenzhen Peacock Plan (20130415095710361) the National High Technology Research and Development Program of China (2014AA10A604-8,2014AA10A601) 

主　　题：Rice Low-nitrogen stress Metabolite Nitrogen metabolism 

摘      要：Identification of metabolites responsible for tolerance to low nitrogen availability(low-N)will aid in the genetic improvement of rice yield under nitrogen *** this study,a backcross introgression line(G9)and its recurrent parent Shuhui 527(SH527),which show differential responses to low-N stress,were used to identify metabolites associated with lowN tolerance in *** in metabolite contents in the leaves of G9 and SH527 at three growth stages under low-N stress were assessed by gas chromatography–mass *** metabolites,including amino acids and derivatives,were highly enriched in G9 compared with SH527 under the control condition,suggesting that the two genotypes had basal metabolite ***-N stress induced genotype-specific as well as growth stagedependent metabolite *** induced specifically in G9 that were involved in glycolysis and tricarboxylic acid metabolism were enriched at the tillering and grain filling stages,and metabolites involved in nitrogen and proline metabolism were enriched at the booting *** of pyroglutamate,glutamate,2-oxoglutarate,sorbose,glycerate-2-P,and phosphoenolpyruvic acid in G9 suggests that these metabolites could be involved in low-N stress *** results presented here provide valuable information for further elucidation of the molecular mechanisms of low-N tolerance in crops.