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JINGHUA NO. 1——A WINTER WHEAT VARIETY DERIVED FROM POLLEN SPOROPHYTE

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Science China Chemistry 1986年第7期29卷 733-745页

作者：胡道芬袁振东汤云莲刘建平 Beijing Academy of Agricultural Sciences institute of crop breeding and cultivation

46 green plantlets were obtained by inoculating the anthers of Fhybrids of complicated combination (（Lovrin 18×5238-036） F×Hongliang No. 4) from wheat to the medium Nin 1977. Induced percentage of green plantlets was 11.50%. After chromosome doubling treatment, 28 plantlets set seeds. Diversity in morphological characters, growing periods and resistance to diseases were observed in Hgeneration, from which plantline for Jinghua No. 1 was selected for the first time this year. Variety comparative and regional adaptive experiments were performed in subsequent H, H,H, and *** area of planting Jinghua No. 1 amounted to 7000 ha in 1984. The evaluation of Jinghua No. 1 was passed by Beijing Variety Evaluation *** No. 1 is a high yield and high quality new wheat variety. It resists strongly leaf, stem and stripe rust and powdery mildew and possesses good *** development of Jinghua No. 1 took only 7 generations, among which 3 years were spent for hybridiz

关键词： A WINTER WHEAT VARIETY DERIVED FROM POLLEN SPOROPHYTE than

Mapping of a BYDV resistance gene from Thinopyrum intermedium in wheat background by molecular markers

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Science China(Life Sciences) 1999年第6期42卷 663-668,673页

作者：张增艳辛志勇马有志陈孝徐琼芳林志珊 institute of crop breeding and cultivation Chinese Academy of Agricultural Sciences Beijing China

The wheat line H960642 is a homozygous wheat-Thinopyrum intermedium translocation line with resistance to BYDV by genomie in situ hybridization (GISH) and RFLP analysis. The genomie DNA of Th. intermedium was used as a probe, and eonunon wheat genomie DNA as a blocking in GISH experiment. The results showed that the chromosome segments of Th. intermedium were transferred to the distal end of a pair of wheat chromosomes. RFLP analysis indicated that the transloeation line H960642 is a T7DS·7DL-7XL translocation by using 8 probes mapped on the homoeologous group 7 in wheat. The tranalocation breakpoint is located between Xpsr680 and Xpsr965 about 90—99 cM from the centromere. The RFLP markers psr680 and psr687 were closoly linked with the BYDV resistance gene. The gene is located on the distal end of 7XL around Xpsr680 and Xpsr687.

关键词： BYDV Thinopyrum intermedium translocation line GISH RFLP.

GENOME STUDY AND TELOSOMIC ANALYSIS OF THE SINGLE DOMINANT MALE-STERILE Tal GENE IN COMMON WHEAT

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Science in China,Ser.B 1986年第5期29卷 516-526页

作者：刘秉华邓景扬 institute of crop breeding and cultivation Chinese Academy of Agricultural Sciences Beijing

The single dominant male-sterile Tal gene in Taigu wheat (T. aestivum L.) is located on the short arm of 4D chromosome, and its position relative to centromere is calculated, being about 31.1 crossing-over units, by making use of the new process and technique of determinating the genome-located Tal gene, telosmie test and analysis.

关键词： gene Ta GENOME STUDY AND TELOSOMIC ANALYSIS OF THE SINGLE DOMINANT MALE-STERILE Tal GENE IN COMMON WHEAT

Genetic Variation of Inbred Lines of Maize Detected by SSR Markers

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Agricultural Sciences in China 2001年第1期 14-20页

作者： LI Xin-hai FU Jun-hua ZHANG Shi-huang YUAN Li-xing LI Ming-shun The institute of crop breeding and cultivation Chinese Academy of Agoriculturas Sciences Key Laboratory of Crop Breeding and Cultivation Ministry of Agriculture Beijing 100081 The Institute of Crop Breeding and Cultivation Chinese Academy of Agoriculturas Sciences Key Laboratory of Crop Breeding and Cultivation Ministry of Agriculture Beijing 100081 The Institute of Crop Breeding and Cultivation Chinese Academy of Agoriculturas Sciences Key Laboratory of Crop Breeding and Cultivation Ministry of Agriculture Beijing 100081 The Institute of Crop Breeding and Cultivation Chinese Academy of Agoriculturas Sciences Key Laboratory of Crop Breeding and Cultivation Ministry of Agriculture Beijing 100081 The Institute of Crop Breeding and Cultivation Chinese Academy of Agoriculturas Sciences Key Laboratory of Crop Breeding and Cultivation Ministry of Agriculture Beijing 100081

Simple sequence repeats (SSRs) were used to detect genetic variation among 21 maize(Zea mays L. ) inbred lines. Forty-three SSR primers selected from 69 primers gave stable amplification profiles, which could be clearly resolved on 3% Metaphor agarose gel, and produced 127 polymorphic amplified *** average number of alleles per SSR locus was 2.95 with a range from 2 to 7. The polymorphism information content (PIC) for the SSR loci varied from 0.172 to 0.753 with an average of 0.511. Genetic similarities among the 21 lines ranged from 0.480 between the combination of Zhongzi451 vs. K12 up to 0.768 between CA156 vs. Ye478. The cluster analysis showed that 21 inbred lines could be classified into two distinct clusters with several subclusters, which corresponded to the heterotic groups determined by their pedigree *** SSR primers, which had high level of polymorphism, could allow a rapid and efficient identification of 21 inbreds. Consequently, SSR markers could be used for measuring genetic variation of maize inbred lines and assigning them to heterotic groups.

关键词： Maize Simple sequence repeats Genetic variation Heterotic groups Polymorphism information content

A New PCR/LDR-Based Multiplex Functional Molecular Marker for Marker-Assisted breeding in Rice

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Rice science 2021年第1期28卷 6-10页

作者： Chu Huangwei Tu Rongjian Niu Fuan Zhou Jihua Sun Bin Luo Zhongyong Cheng Can Cao Liming institute of crop breeding and cultivation Shanghai Academy of Agricultural SciencesShanghai 201403China

Marker-assisted selection or marker-aided selection(MAS)provides an effective complementary approach for conventional rice breeding with precise and speedy mobilization of target genes into elite genetic *** targeted genes,however,may not be selected in the course of MAS due to the occasional recombination between the marker and the target gene/QTL during the many cycles of meiosis involved in breeding *** leads to failure in the selection of target traits(Gopalakrishnan et al,2008).

关键词： breeding elite Marker

FURTHER STUDY ON THE INHERITANCE OF THE GENIC MALE-STERILE WHEAT OF TAIGU AND THE PRODUCTION OF THE DOMINANT MALE-STERILE OCTOPLOID TRITICALE

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中国科学B辑(英文版) 1985年第6期28卷 608-617页

作者：纪凤高邓景扬 institute of crop breeding and cultivation Chinese Academy of Agricultural Sciences Beijing Beijing

According to the result of the studies of the Taigu genie male-sterile wheat of the predecessor, a series of inductive inference was made at first. We used the Taigu genic malesterile wheat to make cross with rye to produce polyhaploid plant. By chromosome doubling, the dominant male-sterile gene became homozygous under artificial condition. The result of the experiment obtained shows that the expression and segregation ratio of the stamens of the haploid, amphiploid and the hybrid progenies of amphiploid plants well correspond to the inductive inference, so it confirms once again that a single dominant gene determines the male-sterility of the Taigu genic male-sterile *** authors verify that the Tal gene not only can be expressed normally in common wheat’s genetic background, but also can be introduced into the octoploid triticale. The first dominant genic male-sterile octoploid triticale is produced and has been used in triticale breeding program.

关键词： sterile wheat sterility haploid breeding anther chromosome segregation fertility genie

DEVELOPMENT OF COMMON WHEAT GERM-PLASM RESISTANT TO BARLEY YELLOW DWARF VIRUS BY BIOTECHNOLOGY

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Science China Chemistry 1991年第9期34卷 1055-1062页

作者：辛志勇徐惠君陈孝林志珊周广和钱幼亭成卓敏 P. J. LARKIN P. BANKS R. APPELS B. GLARKE AND R. I. S. BRETTELL institute of crop breeding and cultivation GAAS institute of Plant Protection GAAS CSIRO Division of Plant IndustryAustralia

Barley yellow dwarf virus (BYDV) is one of the most serious wheat diseases in China. So far no resistance has been described in common wheat. A certain level of BYDV resistance was found in thirteen Triticeae species. Thinopyrum intermedium, two octoploids derived from TH. intermedium/wheat, Zhong 4 awnless and TAF46, and one disomic addition line, L1 derived from TAF46, showed good resistance to BYDV by enzyme linked immunosorbent assay (ELISA). Two wheat/TA. intermedium translocation lines, CPI 119880 and CPI 119899, showing good BYDV resistance were developed from L1 by using both CSph mutant and tissue culture. It is found that their BYDV resistance was controlled by a single dominant gene. Two cDNA probes pEleAcc3 and pPJN8 (E1-T1) were screened for detecting Th. intermedium DNA in wheat background. A specific band for the DNA of Th. intermedium and its derivatives was found in Southern hybridization. It is also possible to determine the size of the alien segment by comparing the relative density of the specific band. Therefore, this can be used as a marker to identify the BYDV resistance in wheat breeding program.

关键词： wheat yellow dwarf virus barley yellow dwarf virus Thinopyrum intermedium addition line translocation line cDNA probe.

Fine-tuning the spatial allocation of phytohormones for smart canopy, smart breeding, and smart agriculture

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Science China Life Sciences 2025年第1期68卷 309-310页

作者： Tao Zhou Lin Li National Key Laboratory of crop Genetic Improvement Huazhong Agricultural University HuBei HongShan Laboratory

The dual challenges of global climate change and population growth have increased demand for higher crop yields per unit area. The innovative technology of smart agriculture aims to synergize breeding, cultivation, and production through the application of biological and informatics technologies. Historically, the “Green Revolution” introduced semidwarf varieties of certain key crops by utilizing mutations affecting gibberellin pathways, which significantly increased planting densities and yields.

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Variation of wheatgrass chromosomes in wheat-wheatgrass alien addition line"TAI-27"revealed by fluorescence in situ hybridization(FISH)

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Science China(Life Sciences) 1998年第4期41卷 367-371,449页

作者： HAN Fangpu ZHANG Xiangqi BUXiuling HE Mengyuan HAO Shui MA Youzhi XIN Zhiyong institute of Genetics and Cytobgy Northeast Noma1 UniversityChangchun 130024China institute of crop breeding and cultivation Chinese Academy of Agricultural SciencesCrop Geneties and BreedingMinistry of Agriculture Key LaboratoryBeijing 100081China

The karyotype of the primary wheat wheatgrass alien addition line TAI 27 was 2 n=44 in which all of the chromosomes were metacentric and ***,in the progeny of TAI 27 a pair of chromosomes had become small chromosomes in the two morphologically different *** in situ hybridization(FISH)technique was used to analyze the two different *** observations indicate that a pair of small chromosomes in one variation line are from *** another variation line,a pair of small chromosomes are also from wheatgrass,while another pair of wheatgrass chromosomes have substituted the wheat *** 27 and its variant lines showed a high level of resistance to barley yellow dwarf virus(BYDV).The possible explanation for such a variation and the potential use of the variant lines were discussed briefly.

关键词： wheat wheatgrass alien addition line alien addition substitution line fluorescence in situ hybridization(FISH)