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A stage-specific cell-manipulation platform for inducing endothelialization on demand

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National Science Review 2020年第3期7卷 629-643页

作者： Qilong Zhao Juan Wang Yunlong Wang Huanqing Cui Xuemin Du institute of biomedical& health engineering Shenzhen Institutes of Advanced TechnologyChinese Academy of Sciences

Endothelialization is of great significance for vascular remodeling, as well as for the success of implanted vascular grafts/stents in cardiovascular disease treatment. However, desirable endothelialization on synthetic biomaterials remains greatly challenging owing to extreme difficulty in offering dynamic guidance on endothelial cell(EC) functions resembling the native extracellular matrix-mediated effects. Here, we demonstrate a bilayer platform with near-infrared-triggered transformable topographies, which can alter the geometries and functions of human ECs by tunable topographical cues in a remote-controlled manner, yet cause no damage to the cell viability. The migration and the adhesion/spreading of human ECs are respectively promoted by the temporary anisotropic and permanent isotropic topographies of the platform in turn, which appropriately meet the requirements of stage-specific EC manipulation for endothelialization. In addition to the potential of promoting the development of a new generation of vascular grafts/stents enabling rapid endothelialization, this stage-specific cell-manipulation platform also holds promise in various biomedical fields, since the needs for stepwise control over different cell functions are common in wound healing and various tissue-regeneration processes.

关键词： biomaterials tissue engineering shape-memory polymer topographical conversion endothelialization

Highly efficient labelling of extracellular vesicles for enhanced detection on a microfluidic platform

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Chinese Chemical Letters 2024年第2期35卷 441-445页

作者： Shi Hu Rui Hao Zitong Yu Huitao Zhang Hui Yang Bionic Sensing and Intelligence Center Institute of Biomedical and Health EngineeringShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China

Developing precise extracellular vesicles(EVs)labelling techniques with minimal disturbance is of great importance to the follow-up EVs detection and analysis.However,currently available methods such as using probes to conjugate phospholipids or membrane proteins have certain limitations due to EV steric hindrance,dye aggregation,etc.Here,we present a microfluidic platform to enhance EVs’labelling efficiency and improve their detection.This platform provides excellent sample throughput and high-efficiency EV labelling at lower label concentrations with an optimized flowing rate.Flow cytometry analysis(FCM)and cellular uptake results show that EV labelling by utilizing this platform possesses the merits of a higher labelling efficiency with 64.1%relative improvement than conventional co-incubation method and a lower background noise.Moreover,this technique maintains EVs’size,morphology and biological activities.After the recipient cells uptake the EVs treated by the microfluidic platform,the spatial and temporal distribution of EVs in the cells are clearly observed.These results demonstrate that our method holds great potential in efficient labelling of EVs,which is essential to subsequent EV quantification and analysis.

关键词： Microfluidic platform Extracellular vesicles Labelling PKH dye Flow cytometry

A magnetically enabled simulation of microgravity represses the auxin response during early seed germination on a microfluidic platform

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Microsystems & Nanoengineering 2022年第1期8卷 97-109页

作者： Jing Du Lin Zeng Zitong Yu Sihui Chen Xi Chen Yi Zhang Hui Yang Laboratory of biomedical Microsystems and Nano Devices Center for Bionic Sensing and IntelligenceInstitute of Biomedical and Health EngineeringShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China Center for Medical AI Institute of Biomedical and Health EngineeringShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China

For plants on Earth,the phytohormone auxin is essential for gravitropism-regulated seedling establishment and plant growth.However,little is known about auxin responses under microgravity conditions due to the lack of a tool that can provide an alteration of gravity.In this paper,a microfluidic negative magnetophoretic platform is developed to levitate Arabidopsis seeds in an equilibrium plane where the applied magnetic force compensates for gravitational acceleration.With the benefit of the microfluidic platform to simulate a microgravity environment on-chip,it is found that the auxin response is significantly repressed in levitated seeds.Simulated microgravity statistically interrupts auxin responses in embryos,even after chemical-mediated auxin alterations,illustrating that auxin is a critical factor that mediates the plant response to gravity alteration.Furthermore,pretreatment with an auxin transportation inhibitor(N-1-naphthylphthalamic acid)enables a decrease in the auxin response,which is no longer affected by simulated microgravity,demonstrating that polar auxin transportation plays a vital role in gravity-regulated auxin responses.The presented microfluidic platform provides simulated microgravity conditions in an easy-to-implement manner,helping to study and elucidate how plants correspond to diverse gravity conditions;in the future,this may be developed into a versatile tool for biological study on a variety of samples.

关键词： magnetic auxin microgravity

Multiphasic scaffolds for the repair of osteochondral defects:Outcomes of preclinical studies

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Bioactive Materials 2023年第9期27卷 505-545页

作者： Rouyan Chen Jasmine Sarah Pye Jiarong Li Christopher B.Little Jiao Jiao Li Kolling institute Faculty of Medicine and HealthThe University of SydneyNSW2065Australia School of Electrical and Mechanical engineering Faculty of SciencesEngineering and TechnologyThe University of AdelaideSA5005Australia School of biomedical engineering Faculty of Engineering and ITUniversity of Technology SydneyNSW2007Australia

Osteochondral defects are caused by injury to both the articular cartilage and subchondral bone within skeletal joints. They can lead to irreversible joint damage and increase the risk of progression to osteoarthritis. Current treatments for osteochondral injuries are not curative and only target symptoms, highlighting the need for a tissue engineering solution. Scaffold-based approaches can be used to assist osteochondral tissue regeneration, where biomaterials tailored to the properties of cartilage and bone are used to restore the defect and minimise the risk of further joint degeneration. This review captures original research studies published since 2015, on multiphasic scaffolds used to treat osteochondral defects in animal models. These studies used an extensive range of biomaterials for scaffold fabrication, consisting mainly of natural and synthetic polymers. Different methods were used to create multiphasic scaffold designs, including by integrating or fabricating multiple layers, creating gradients, or through the addition of factors such as minerals, growth factors, and cells. The studies used a variety of animals to model osteochondral defects, where rabbits were the most commonly chosen and the vast majority of studies reported small rather than large animal models. The few available clinical studies reporting cell-free scaffolds have shown promising early-stage results in osteochondral repair, but long-term follow-up is necessary to demonstrate consistency in defect restoration. Overall, preclinical studies of multiphasic scaffolds show favourable results in simultaneously regenerating cartilage and bone in animal models of osteochondral defects, suggesting that biomaterials-based tissue engineering strategies may be a promising solution.

关键词： Osteochondral defects Tissue engineering Multiphasic scaffolds Biomaterials Animal models

A cadaveric breast cancer tissue phantom for phase-contrast X-ray imaging applications

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Animal Models and Experimental Medicine 2023年第5期6卷 427-432页

作者： Cody C.Rounds Chengyue Li Wei Zhou Kenneth M.Tichauer Jovan G.Brankov biomedical engineering Illinois Institute of TechnologyChicagoIllinoisUSA Medical Imaging Research Center Illinois Institute of TechnologyChicagoIllinoisUSA Electrical and Computer engineering Illinois Institute of TechnologyChicagoIllinoisUSA

Background:As mammography X-ray imaging technologies advance and provide elevated contrast in soft tissues,a need has developed for reliable imaging phantoms for use in system design and component calibration.In advanced imaging modalities such as refraction-based methods,it is critical that developed phantoms capture the biological details seen in clinical precancerous and cancerous cases while minimizing artifacts that may be caused due to phantom production.This work presents the fabrication of a breast tissue imaging phantom from cadaveric breast tissue suitable for use in both transmission and refraction-enhanced imaging systems.Methods:Human cancer cell tumors were grown orthotopically in nude athymic mice and implanted into the fixed tissue while maintaining the native tumor/adipose tissue interface.Results:The resulting human–murine tissue hybrid phantom was mounted on a clear acrylic housing for absorption and refraction X-ray imaging.Digital breast tomosynthesis was also performed.Conclusion:Both attenuation-based imaging and refraction-based imaging of the phantom are presented to confirm the suitability of this phantom's use in both imaging modalities.

关键词： breast tumors digital mammography imaging phantoms orthotopic animal models phasecontrast X-ray imaging

Numerical study of cavitating flow in orifices and its effect on spray characteristics

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Journal of Hydrodynamics 2018年第5期30卷 908-919页

作者： Morteza Ghorbani Mechatronics engineering Program Faculty of Engineering and Natural Science Sabanci University Department of biomedical engineering and health Systems KTH Royal Institute of Technology

The bubbly flow regime inside orifices has significant effects on several applications, and studying its trend along an orifice could be helpful in identifying the flow mechanism in various situations. The flow regime inside an orifice depends on the situation which has been specified for the orifice. Orifice geometry has a considerable effect on bubbly flow in injectors. Meanwhile, spray characteristics are influenced by the fuel flow inside an orifice, which has strong effects on the mixture of fuel-air. In this study, spray characteristics are studied for different values of the orifice angle. The cavitation phenomenon which occurs inside an orifice varies in intensity and patterns at different angles of the orifice and consequently has diverse effects on spray characteristics. The governing equations are solved by the SIMPLE algorithm. The spray flow is modeled by the discrete droplet method（DDM）, the droplet breakup is modeled by the WAVE model, and the primary breakup is modeled by the DIESEL BREAK UP model. In order to generate cavitation phenomenon inside orifices and investigate its effect on spray characteristics, the angle of orifice with respect to the injector body is varied and the problem is studied for different angles of orifice.

关键词： Cavitation iinjector angle microchannel spray

Astrocyte-Mediated Myelin Phagocytosis in Ischemia

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Neuroscience Bulletin 2023年第1期39卷 167-169页

作者： Luodan Yang Dongyu Zhang Quanguang Zhang Department of Neurology Louisiana State University Health Sciences CenterShreveportLA71103USA Department of biomedical engineering Georgia Institute of TechnologyAtlantaGA30332USA

The myelin sheath-supporting cells,oligodendrocytes and Schwann cells,are susceptible toischemia.Therefore,ischemic stroke is usually characterized by evident demyelination^([l]).Although oligodendrocytes are the major player in myelination,glial cells,including microglia and astrocytes,also contribute to myelination and demyelination^([2]).In the early stages of ischemic stroke,microgliamediated myelin debris removal through phagocytosis has been widely studied^([3]).

关键词： myelin cytes debris

Versatile Ti_(3)C_(2)T_(x)MXene for free-radical scavenging

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Nano Research 2022年第3期15卷 2558-2566页

作者： Jiang Liu Wei Lu Xifeng Lu Lu Zhang Haifeng Dong Yingchun Li Flexible Printed Electronics technology Center and School of Science Harbin Institute of Technology(Shenzhen)Shenzhen 518055China Department of Physiology Health Science CenterShenzhen UniversityShenzhen 518055China Marshall Laboratory of biomedical engineering Research Center for Biosensor and NanotheranosticSchool of Biomedical EngineeringShenzhen UniversityShenzhen 518055China

MXene,as an emerging two-dimensional(2D)material with ultrathin structure and fascinating physiochemical properties,has been widely explored in broad applications.Versatile functions of MXenes are continuously explored.This work presents distinctive feature of MXene-Ti_(3)C_(2)T_(x)nanosheets for free-radical(FRs)scavenging that never reported before.We demonstrated the mechanism and equation in regard to the reaction between Ti_(3)C_(2)T_(x)and H_(2)O_(2),which was applied to design colorimetric H_(2)O_(2)strip assay with good performance.The good FRs scavenging capability of Ti_(3)C_(2)T_(x),including a series of reactive oxygen species(ROS)and reactive nitrogen species(RNS),was systemically confirmed.The antioxidation capability of Ti_(3)C_(2)T_(x)for protecting cells from oxidative damage was demonstrated using the oxidative damage model of alpha mouse liver 12(AML-12)cells.This original work provides huge opportunities for MXenes in FR-related biomedical applications.

关键词： MXene hydrogen peroxide colorimetric sensor reactive oxygen species anti-oxidative damage

近红外二区荧光可视化超声联合吲哚菁绿微泡开放血脑屏障增强脑胶质瘤光热治疗

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Science Bulletin 2022年第22期67卷 2316-2326页

作者：梁思敏胡德红黎国锋高笃阳李飞郑海荣潘敏盛宗海 Department of Ultrasonography Shenzhen Hospital(Futian)of Guangzhou University of Chinese MedicineShenzhen 518034China Paul C.Lauterbur Research Center for biomedical Imaging CAS Key Laboratory of Health InformaticsShenzhen Key Laboratory of Ultrasound Imaging and TherapyInstitute of Biomedical and Health EngineeringShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China School of biomedical engineering Guangdong Medical UniversityDongguan 523808China

聚焦超声开放血脑屏障是增强胶质母细胞瘤治疗的关键,然而,缺乏一种高时空分辨率的体内成像方法用于原位同步监测血脑屏障开放过程.本文报道了使用吲哚菁绿掺杂的微泡(MBs-ICG)可视化聚焦超声开放血脑屏障和增强对抗胶质母细胞瘤的光热... 详细信息

聚焦超声开放血脑屏障是增强胶质母细胞瘤治疗的关键,然而,缺乏一种高时空分辨率的体内成像方法用于原位同步监测血脑屏障开放过程.本文报道了使用吲哚菁绿掺杂的微泡(MBs-ICG)可视化聚焦超声开放血脑屏障和增强对抗胶质母细胞瘤的光热治疗方法.结果显示, MBs-ICG具有明亮的近红外二区荧光,超声造影成像和超声响应特性.利用其互补的成像特性, MBs-ICG可实现脑血管的高分辨率(~168.9μm)近红外二区荧光成像、大穿透深度(~7 mm)超声造影成像.通过近红外二区荧光成像, MBs-ICG联合聚焦超声可原位同步可视化血脑屏障开放,信噪比为6.2±1.2.此外,在聚焦超声辐照下, MBs-ICG转化为脂质ICG纳米颗粒,并在10 min内迅速进入肿瘤组织,成功用于增强原位胶质母细胞瘤小鼠的光热治疗.多功能MBs-ICG为监测血脑屏障开放和增强胶质母细胞瘤治疗提供了一种新的范式.

关键词：吲哚菁绿光热治疗胶质母细胞瘤血脑屏障聚焦超声荧光成像近红外脑胶质瘤