检索结果-南通市图书馆

LPA_(1) antagonist-derived LNPs deliver A20 mRNA and promote anti-fibrotic activities

Nano Research 2024年第10期17卷 9095-9102页

作者： Jingyue Yan Diana D.Kang Chang Wang Xucheng Hou Shi Du Siyu Wang Yonger Xue Zhengwei Liu Haoyuan Li Yichen Zhong Binbin Deng David W.McComb Yizhou Dong Division of Pharmaceutics&pharmacology College of PharmacyThe Ohio State UniversityColumbusOH 43210USA Icahn Genomics institute Precision Immunology InstituteDepartment of Immunology and ImmunotherapyDepartment of Oncological SciencesTisch Cancer InstituteBiomedical Engineering and Imaging InstituteFriedman Brain InstituteIcahn School of Medicine at Mount SinaiNew YorkNY 10029USA Center for Electron Microscopy and Analysis The Ohio State UniversityColumbusOH 43212USA department of Materials Science and engineering The Ohio State UniversityColumbusOH 43210USA

Activated fibroblasts are major mediators of pulmonary *** are generally found in the connective tissue but upon activation can generate excess extracellular matrix(ECM)in the lung interstitial ***,fibroblasts are one of the most targeted cells for treating idiopathic pulmonary fibrosis(IPF).Here,we develop an anti-fibrotic platform that can modulate both the lysophosphatidic acid receptor 1(LPA_(1))and the inflammatory pathway through tumor necrosis factorα-induced protein 3(TNFAIP3,also known as A20)in ***,we synthesized a series of LPA_(1) antagonists,AM095 and AM966,derived amino lipids(LA lipids)which were formulated into LA-lipid nanoparticles(LA-LNPs)encapsulating ***,LA5-LNPs,with AM966 head group and biodegradable acetal lipid tails,showed efficient A20 mRNA delivery to lung fibroblasts in vitro(80.2%±1.5%)and ex vivo(17.2%±0.4%).When treated to primary mouse lung fibroblasts(MLF),this formulation inhibited fibroblast migration and collagen production,thereby slowing the progression of ***,LA5-LNPs encapsulated with A20 mRNA is a novel platform offering a potential approach to regulate fibroblast activation for the treatment of IPF.

关键词： lysophosphatidic acid receptor 1(LPA_(1))antagonist tumor necrosis factorα-induced protein 3(A20) lung fibrosis lipid nanoparticles mRNA

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Macular carotenoid supplementation improves disability glare performance and dynamics of photostress recovery

引用

Eye and Vision 2016年第1期3卷 231-238页

作者： James M.Stringham Kevin J.O’Brien Nicole T.Stringham Nutritional Neuroscience Laboratory Department of Physiology and PharmacologyUniversity of GeorgiaAthensGA 30602USA Vision Sciences Laboratory Department of PsychologyUniversity of GeorgiaAthensGA 30602USA Interdisciplinary Neuroscience Program Biomedical Health Sciences InstituteUniversity of GeorgiaAthensGA 30602USA

Background:The so-called macular carotenoids(MC)lutein(L),zeaxanthin(Z),and meso-zeaxanthin(MZ)comprise the diet-derived macular pigment(MP).The purpose of this study was to determine effects of MC supplementation on the optical density of MP(MPOD),repeated-exposure photostress recovery(PSR),and disability glare(DG)***:This was a double-blind,placebo-controlled ***-nine young(mean age=21.7),healthy volunteers participated in this *** supplemented their daily diet with either 10 mg L+2 mg total Z(1 mg Z+1 mg MZ;n=24),20 mg L+4 mg total Z(2 mg Z+2 mg MZ;n=25),or placebo(n=10)for 12 *** primary outcome was a composite measure of visual performance in glare,defined by change in DG and *** outcomes included MPOD and visual *** primary endpoint for outcomes was 12 *** was assessed with customized heterochromatic flicker *** times for an 8 cycle/degree,15%contrast Gabor patch target were determined after each of five successive exposures to intense LED *** threshold was defined as the intensity of a ring of lights through which subjects were able to maintain visibility of the aforementioned *** of all parameters were conducted at baseline,6 months,and 12 ***-measures ANOVA,and Pearson product-moment correlations were used to determine statistically significant correlations,and changes within and between ***:MPOD for subjects in both supplementation groups increased significantly versus placebo at both 6-and 12-month visits(p<0.001 for all).Additionally,PSR times and DG thresholds improved significantly from baseline compared to placebo at 6-and 12-month visits(p<0.001 for all).At baseline,MPOD was significantly related to both DG thresholds(r=0.444;p=0.0021)and PSR times(r=-0.56;p<0.001).As a function of MPOD,the repeated-exposure PSR curves became more asymptotic,as opposed to *** change in subjects’DG thresholds were significantly related to chang

关键词： Lutein Zeaxanthin Mesozeaxanthin Visual performance Photostress recovery Disability glare Macular pigment Visual cycle

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Towards a Medium/High Load-Bearing Scaffold Fabrication System

引用

Tsinghua Science and Technology 2009年第S1期14卷 13-19页

作者： GIBSON Ian SAVALANI Monica M LAM Christopher X F OLKOWSKI Radoslow EKAPUTRA Andrew K TAN Kim Cheng HUTMACHER Dietmar W Faculty of engineering National University of Singapore department of Biophysics and Human physiology Medical University of Warsaw Warsaw 02-091 Poland Temasek engineering School Temasek Polytechnic institute of Health and biomedical Innovation Queensland University of Technology Brisbane QL-4000 Australia

This paper describes fabrication of scaffolds for load-bearing applications, with primary consideration from the manufacturing perspective. An extrusion device, inspired by the FDM process, was used to create scaffolds from a variety of different polymeric materials and mixtures. The effectiveness of these scaffolds to host cells for bone regeneration has been investigated. This ongoing work has generated significant insight into the future direction of research and the possibilities of developing scaffolds for medium/high load-bearing applications.

关键词： tissue engineering load-bearing bone engineering scaffolds

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Influence of Electromagnetic Fields on Bone Fracture in Rats: Role of CAPE

引用

biomedical and Environmental Sciences 2009年第2期22卷 157-160页

作者： EKREM CICEK OSMAN GOKALP REMZI VAROL GOKHAN CESUR department of Physics Faculty of Arts and ScienceMehmet Akif Ersoy UniversityBurdurTurkey Suleyman Demirel University department of pharmacology Suleyman Demirel University department of Mechanical engineering Faculty of EngineeringSuleyman Demirel University department of physiology Faculty of Medicine Suleyman Demirel University

Objective To study the effects of radiation emitted by mobile phones on bone strength and caffeic acid phenethyl ester （CAPE） on the changes induced by radiation. Methods Forty-eight Sprague-Dawley rats were divided into five groups. Rats in the control group （first group） were left within the experimental setup for 30 min/day for 28 days without radiation exposure. Nine hundred MHz radiation group was broke down into 2 subgroups （group 1/2）. Both subgroups were exposed to radiation for 28 days （30 min/day）. The next group was also divided into 2 subgroups （group 3/4）. Each was exposed to 1800 MHz of radiation for 28 days （30 mirdday）. The third and fifth groups were also treated with CAPE for 28 days. Treatment groups received ip caffeic acid phenethyl ester （10 ktmol/kg per day） before radiation session. Bone fracture was analyzed. Results Breaking force, bending strength, and total fracture energy decreased in the irradiated groups but increased in the treatment groups. Conclusion Radiation and CAPE can significantly improve bone.

关键词： Mobile phone Biomechanical study Caffeic acid phenethyl ester

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Safety evaluation of nanodiamond-doxorubicin complexes in a Naïve Beagle canine model using hematologic,histological,and urine analysis

引用

Nano Research 2022年第4期15卷 3356-3366页

作者： Liping Wang Wenqiong Su Khan Zara Ahmad Xin Wang Ting Zhang Youyi Yu Edward Kai-Hua Chow Dean Ho Xianting Ding State Key Laboratory of Oncogenes and Related Genes Institute for Personalized MedicineSchool of Biomedical EngineeringShanghai Jiao Tong UniversityShanghai 200030China Cancer Science institute of Singapore Yong Loo Lin School of MedicineNational University of SingaporeSingapore 117599Singapore The N.1 institute for Health(N.1) National University of SingaporeSingapore 117456Singapore The institute for Digital Medicine(WisDM) National University of SingaporeSingapore 117456Singapore department of pharmacology National University of SingaporeSingapore 117597Singapore department of biomedical engineering National University of SingaporeSingapore 117583Singapore

While doxorubicin(DOX)is one of the most common chemotherapeutic drugs for treating cancer,use of DOX must be managed carefully due to dose-related ***(ND)drug delivery system conjugated with DOX(NDX)has been reported to enhance treatment efficacy and attenuate toxicity in murine cancer *** addition,extensive biocompatibility studies indicate that NDs seem to be well tolerated in non-human *** the clinical translation of NDX,it is necessary to verify the safety of ND in large *** of nanomedicine drug safety for large animal are not commonly reported,and this work represents a key milestone in bridging earlier advances towards clinical ***,NDs’safety as a drug-delivery platform was evaluated in Naïve Beagle *** study is performed with DOX,ND,and NDX in a dual-gender animal model using intravenous(IV)injection and hepatic portal vein(HPV)injection *** dogs are monitored for their health phenotype changes in continuous 5 *** and urine obtained are for clinical pathology *** results indicate that ND drug delivery platform significantly relieves DOX toxicity for Naïve Beagle dog *** study provides guidance for the pre-clinical safety assessment of NDX therapy at large animal level.

关键词： nanomedicine doxorubicin nanodiamonds safety biodistribution

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Blockade of PD-L1/PD-1 signaling promotes osteo-/odontogenic differentiation through Ras activation

引用

International Journal of Oral Science 2022年第2期14卷 164-173页

作者： So Mi Jeon Je Sun Lim Su Hwan Park Hyung Joon Kim Hyung-Ryong Kim Jong-Ho Lee department of Health Sciences The Graduate School of Dong-A UniversityBusanRepublic of Korea department of Oral physiology Periodontal Diseases Signaling Network Research CenterDental and Life Science InstituteSchool of DentistryPusan National UniversityYangsanRepublic of Korea department of pharmacology College of DentistryJeonbuk National UniversityJeonjuRepublic of Korea department of biomedical Sciences Dong-A UniversityBusanRepublic of Korea

The programmed cell death ligand 1(PD-L1)and its receptor programmed cell death 1(PD-1)deliver inhibitory signals to regulate immunological tolerance during immune-mediated ***,the role of PD-1 signaling and its blockade effect on human dental pulp stem cells(h DPSCs)differentiation into the osteo-/odontogenic lineage remain *** show here that PD-L1 expression,but not PD-1,is downregulated during osteo-/odontogenic differentiation of h ***,PD-L1/PD-1 signaling has been shown to negatively regulate the osteo-/odontogenic differentiation of h ***,depletion of either PD-L1 or PD-1 expression increased ERK and AKT phosphorylation levels through the upregulation of Ras enzyme activity,which plays a pivotal role during h DPSCs osteo-/odontogenic *** with nivolumab(a human anti-PD-1 monoclonal antibody),which targets PD-1 to prevent PD-L1 binding,successfully enhanced osteo-/odontogenic differentiation of h DPSCs through enhanced Ras activity-mediated phosphorylation of ERK and *** findings underscore that downregulation of PD-L1 expression accompanies during osteo-/odontogenic differentiation,and h DPSCs-intrinsic PD-1 signaling inhibits osteo-/odontogenic *** findings provide a significant basis that PD-1 blockade could be effective immunotherapeutic strategies in h DPSCs-mediated dental pulp regeneration.

关键词： death finding monoclonal

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Therapeutic potential of neuromodulation for demyelinating diseases

引用

Neural Regeneration Research 2021年第2期16卷 214-217页

作者： Elliot H.Choi Chioma Nwakalor Nolan J.Brown Joonho Lee Michael Y.Oh In Hong Yang department of pharmacology School of MedicineCase Western Reserve UniversityClevelandOHUSA department of Ophthalmology Gavin Herbert Eye InstituteSchool of MedicineUniversity of CaliforniaIrvineCAUSA department of Neurological Surgery University of CaliforniaIrvineCAUSA department of Mechanical engineering and engineering Science Center for Biomedical Engineering and ScienceUniversity of North Carolina at CharlotteCharlotteNCUSA University of Rochester School of Medicine and Dentistry RochesterNYUSA

Neuromodulation represents a cutting edge class of both invasive and non-invasive therapeutic methods which alter the activity of ***,several different techniques have been developed-or are currently being investigated–to treat a wide variety of neurological and neuropsychiatric ***,in vivo and in vitro studies have revealed that neuromodulation can also induce myelination,meaning that it could hold potential as a therapy for various demyelinating diseases including multiple sclerosis and progressive multifocal *** findings come on the heels of a paradigm shift in the view of myelin's role within the nervous system from a static structure to an active co-regulator of central nervous system plasticity and participant in neuron-mediated *** the present review,we highlight several of the recent findings regarding the role of neural activity in altering myelination including several soluble and contact-dependent factors that seem to mediate neural activitydependent *** also highlight several considerations for neuromodulatory techniques,including the need for further research into spatiotemporal precision,dosage,and the safety and efficacy of transcranial focused ultrasound stimulation,an emerging neuromodulation *** the field of neuromodulation continues to evolve,it could potentially bring forth methods for the treatment of demyelinating diseases,and as such,further investigation into the mechanisms of neuron-dependent myelination as well as neuro-imaging modalities that can monitor myelination activity is warranted.

关键词： central nervous system deep brain stimulation myelination neural activity oligodendrocyte optogenetic stimulation transcranial electrical stimulation transcranial focused ultrasound stimulation transcranial magnetic stimulation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

p85S6K sustains synaptic GluA1 to ameliorate cognitive deficits in Alzheimer’s disease

引用

Translational Neurodegeneration 2023年第1期12卷 890-907页

作者： Jia-Bing Li Xiao-Yu Hu Mu-Wen Chen Cai-Hong Xiong Na Zhao Yan-Hui Ge Hao Wang Xiao-Ling Gao Nan-Jie Xu Lan-Xue Zhao Zhi-Hua Yu Hong-Zhuan Chen Yu Qiu department of pharmacology and Chemical Biology Shanghai Jiao Tong University School of MedicineShanghai 200025China institute of Interdisciplinary Integrative biomedical Research Shuguang HospitalShanghai University of Traditional Chinese MedicineShanghai 201210China Collaborative Innovation Center for Brain Science Department of Anatomy and PhysiologyShanghai Jiao Tong University School of MedicineShanghai 200025China

Background Ribosomal protein S6 kinase 1(S6K1)is a serine-threonine kinase that has two main isoforms:p70S6K(70-kDa isoform)and p85S6K(85-kDa isoform).p70S6K,with its upstream mammalian target of rapamycin(mTOR),has been shown to be involved in learning and memory and participate in the pathophysiology of Alzheimer’s dis-ease(AD).However,the function of p85S6K has long been neglected due to its high similarity to *** role of p85S6K in learning and memory is still largely *** We fractionated the postsynaptic densities to illustrate the differential distribution of p85S6K and *** was performed to unveil interactions between p85S6K and the GluA1 subunit of AMPA *** roles of p85S6K in synaptic targeting of GluA1 and learning and memory were evaluated by specific knockdown or overexpression of p85S6K followed by a broad range of methodologies including immunofluorescence,Western blot,in situ proximity ligation assay,morphological staining and behavioral ***,the expression level of p85S6K was measured in brains from AD patients and AD model *** p85S6K,but not p70S6K,was enriched in the postsynaptic ***,knockdown of p85S6K resulted in defective spatial and recognition *** addition,p85S6K could interact with the GluA1 subunit of AMPA receptor through synapse-associated protein 97 and A-kinase anchoring protein 79/*** studies demonstrated that p85S6K could directly phosphorylate GluA1 at Ser845 and increase the amount of GluA1 in syn-apses,thus sustaining synaptic function and spine ***,p85S6K was found to be specifically decreased in the synaptosomal compartment in the brains of AD patients and AD *** of p85S6K ameliorated the synaptic deficits and cognitive impairment in transgenic AD model *** These results strongly imply a significant role for p85S6K in maintaining synaptic and cognitive function by interacting with Gl

关键词： Alzheimer’s disease Cognition GluA1 subunit of AMPA receptors Ribosomal S6 protein kinase 1 85 kDa isoform

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

engineering LNPs with polysarcosine lipids for mRNA delivery

引用

Bioactive Materials 2024年第7期37卷 86-93页

作者： Diana D.Kang Xucheng Hou Leiming Wang Yonger Xue Haoyuan Li Yichen Zhong Siyu Wang Binbin Deng David W.McComb Yizhou Dong Icahn Genomics institute Precision Immunology InstituteDepartment of Immunology and ImmunotherapyDepartment of Oncological SciencesTisch Cancer InstituteFriedman Brain InstituteBiomedical Engineering and Imaging InstituteIcahn School of Medicine at Mount SinaiNew YorkNY10029USA Division of Pharmaceutics&pharmacology College of PharmacyThe Ohio State UniversityColumbusOH43210USA Center for Electron Microscopy and Analysis The Ohio State UniversityColumbusOH43212USA department of Materials Science and engineering The Ohio State UniversityColumbusOH43210USA

Since the approval of the lipid nanoparticles(LNP)-mRNA vaccines against the SARS-CoV-2 virus,there has been an increased interest in the delivery of mRNA through ***,current LNP formulations contain PEG lipids,which can stimulate the generation of anti-PEG *** presence of these antibodies can potentially cause adverse reactions and reduce therapeutic efficacy after *** the widespread deployment of the COVID-19 vaccines,the increased exposure to PEG may necessitate the evaluation of alternative LNP formulations without PEG *** this study,we investigated a series of polysarcosine(pSar)lipids as alternatives to the PEG lipids to determine whether pSar lipids could still provide the functionality of the PEG lipids in the ALC-0315 and SM-102 LNP *** found that complete replacement of the PEG lipid with a pSar lipid can increase or maintain mRNA delivery efficiency and exhibit similar safety profiles in vivo.

关键词： Lipid nanoparticles mRNA Polyethylene glycol(PEG) Polysarcosine(pSar)

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Optimization and comparison of two different 3D culture methods to prepare cell aggregates as a bioink for organ printing

引用

BIOCELL 2012年第1期36卷 37-45页

作者： RANA IMANI SHAHRIAR HOJJATI EMAMI HOSSEIN FAKHRZADEH NAFISEH BAHEIRAEI ALI M SHARIFI department of biomedical engineering Amirkabir University of TechnologyTehranIran Endocrinology and Metabolism Research institute Tehran University of Medical SciencesTehranIran Razi institute for Drug research Department of PharmacologySchool of MedicineTehran University of Medical SciencesTehranIran department of Tissue engineering and Cell therapy School of advanced sciences in MedicineTehran University of Medical Sciences.TehranIran

The ultimate goal of tissue engineering is to design and fabricate functional human tissues that are similar to natural cells and are capable of *** of cell aggregates is one of the important steps in 3D tissue engineering technology,particularly in organ *** simple methods,hanging drop(HD)and conical tube(CT)were utilized to prepare cell *** size and viability of the aggregates obtained at different initial cell densities and pre-culture duration were *** proliferative ability of the cell aggregates and their ability to spread in culture plates were also *** both methods,the optimum average size of the aggregates was less than 500μ*** aggregates were smaller than HD aggregates.5,000 cells per drop HD aggregates showed a marked ability to attach and spread on the culture *** proliferative ability reduced when the initial cell density was *** these methods,we found that the HD method having better size controlling ability as well as enhanced ability to maintain higher rates of viability,spreading,and *** conclusion,smaller HD aggregates might be a suitable choice as building blocks for making bioink particles in bioprinting technique.

关键词： tissue engineering hanging drop conical tube bioprinting

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：