检索结果-南通市图书馆

A New Ecdysteroid from Rhaponticum uniflorum

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2001年第9期12卷 797-798页

作者： Yong Hong ZHANG Xin Pu LI Zhi Gang LU Han Qing WANG Lanzhou institute of Chemical Physics Chinese Academy of SciencesLanzhou 730000 Lanzhou institute of animal Science and animal Medicine Lanzhou 730050

A new phytoecdysteroid was isolated from the root of Rhaponticum uniflorum. Its structure has been shown to be 2, 3, 20, 22-diacetonide ajugasterone.

关键词： Rhaponticum uniflorum compositae 2, 3, 20, 22-diacetonide ajugasterone

Effect of dietary supplementation with mulberry(Morus alba L.) leaves on the growth performance,meat quality and antioxidative capacity of finishing pigs

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2019年第1期18卷 143-151页

作者： ZENG Zhu JIANG Jun-jie YU Jie MAO Xiang-bing YU Bing CHEN Dai-wen Key Laboratory for animal Disease-Resistant Nutrition Ministry of Education/Institute of Animal NutritionSichuan Agricultural University

The present study was conducted to evaluate the effect of dietary mulberry(Morus alba L.) leaves powder(MLP) supplementation on meat quality of finishing pigs.A total of 40 Duroc×Landrace×Yorkshire pigs(initial body weight of(40.5±0.63) kg) were randomly allotted into two treatments,fed either with control diet or 15% MLP diet for 85 *** results showed that MLP diet decreased(P≤0.05) average daily gain(ADG) and increased(P<0.05) feed/gain ratio(F/G) in the finishing and whole *** diet also decreased(P<0.05) carcass weight,dressing percentage,last rib and average backfat ***,MLP diet increased(P<0.05) intramuscular fat(IMF) content,decreased(P<0.05) shear force,cooking loss and drip *** addition,MLP diet increased(P<0.05) total antioxidative capacity,glutathione peroxidase and tended(P<0.10) to increase total superoxide dismutase in *** longissimus thoracis,myosin heavy chain(MyHC) I and IIa mRNA levels were increased(P≤0.05) for MLP *** conclusion,15% MLP supplementation reduced the growth performance and carcass traits,but improved meat quality of finishing pigs possibly through the change of myofiber characteristics,enhancement of antioxidative capacity and increase of IMF.

关键词： mulberry leaves powder meat quality myofibers antioxidation pigs

Reorganization of 3D genome architecture across wild boar and Bama pig adipose tissues

在线全文

学校读者我要写书评

暂无评论

Journal of animal Science and Biotechnology 2022年第4期13卷 1011-1027页

作者： Jiaman Zhang Pengliang Liu Mengnan He Yujie Wang Hua Kui Long Jin Diyan Li Mingzhou Li institute of animal Genetics and Breeding College of Animal Science and TechnologySichuan Agricultural UniversityChengdu 611130China

Background:A growing body of evidence has revealed that the mammalian genome is organized into hierarchical layers that are closely correlated with and may even be causally linked with variations in gene *** studies have characterized chromatin organization in various porcine tissues and cell types and compared them among species and during the early development of ***,how chromatin organization differs among pig breeds is poorly ***:In this study,we investigated the 3D genome organization and performed transcriptome characterization of two adipose depots(upper layer of backfat[ULB]and greater omentum[GOM])in wild boars and Bama pigs;the latter is a typical indigenous pig in *** found that over 95%of the A/B compartments and topologically associating domains(TADs)are stable between wild boars and Bama *** contrast,more than 70%of promoterenhancer interactions(PEIs)are dynamic and widespread,involving over a thousand *** in chromatin structure are associated with changes in the expression of genes that are involved in widespread biological functions such as basic cellular functions,endocrine function,energy metabolism and the immune *** 95%and 97%of the genes associated with reorganized A/B compartments and PEIs in the two pig breeds differed between GOM and ULB,***:We reported 3D genome organization in adipose depots from different pig *** a comparison of Bama pigs and wild boar,large-scale compartments and TADs were mostly conserved,while fine-scale PEIs were extensively *** chromatin architecture in these two pig breeds was reorganized in an adipose depotspecific *** results contribute to determining the regulatory mechanism of phenotypic differences between Bama pigs and wild boar.

关键词： 3D genome organization A/B compartments Adipose tissues PEI Pig breeds TAD

Genome-wide differences in DNA methylation changes in caprine ovaries between oestrous and dioestrous phases

在线全文

学校读者我要写书评

暂无评论

Journal of animal Science and Biotechnology 2019年第1期10卷 25-32页

作者： Xiaopeng An Haidong Ma Peng Han Chao Zhu Binyun Cao Yueyu Bai College of animal Science and Technology Northwest A&F University animal Health Supervision institute of Henan Province

Background: DNA methylation plays a vital role in *** genome DNA methylation changes during the oestrous phase(ES) and dioestrous phase(DS) in the ovaries of Guanzhong dairy goats were investigated using bisulphite sequencing to understand the molecular biological mechanisms of these goats' oestrous ***: We discovered distinct genome-wide DNA methylation patterns in ES and DS ovaries.A total of 26,910 differentially methylated regions were upregulated and 21,453 differentially methylated regions were downregulated in the ES samples compared with the DS samples(P-values ≤0.05 and fold change of methylation ratios ≥2).Differentially methylated region analysis showed hypomethylation in the gene body regions and hypermethylation in the joining region between upstream regions and gene *** methylation ratios of the STAR,FGF2,FGF12,BMP5 and SMAD6 genes in the ES samples were lower than those of the DS samples(P-values≤0.05 and fold change of methylation ratios ≥2).Conversely,the methylation ratios of the EGFR,TGFBR2,IGF2 BP1 and MMD2 genes increased in the ES samples compared with the DS *** addition,223 differentially methylated genes were found in the GnRH signalling pathway(KO04912),ovarian steroidogenesis pathway(KO04913),oestrogen signalling pathway(KO04915),oxytocin signalling pathway(KO04921),insulin secretion pathway(KO04911) and MAPK signalling pathway(KO04010).Conclusions: This study is the first large-scale comparison of the high-resolution DNA methylation landscapes of oestrous and dioestrous ovaries from dairy *** studies and our investigations have shown that the NR5 A2,STAR,FGF2 and BMP5 genes might have potential application value in regulating caprine oestrus.

关键词： Differentially methylated genes Gene ontology Oestrous cycle Pathway

Fat deposition and partitioning for meat production in cattle and sheep

在线全文

学校读者我要写书评

暂无评论

animal Nutrition 2024年第2期17卷 376-386页

作者： Shimin Liu Yanyan Yang Hailing Luo Wenjie Pang Graeme B.Martin The UWA institute of Agriculture The University of Western Australia institute of animal Husbandry of Inner Mongolia Academy of Agricultural and animal Husbandry Sciences College of animal Science and Technology of China Agricultural University Resonance Health Burswood

In markets for beef and sheep meat, an appropriate level of intramuscular fat(IMF) is highly desirable for meat-eating quality, but strategies to improve it usually lead to an undesirable excess in carcase fat,presenting a major challenge to livestock producers. To solve this problem, we need to understand the partitioning of fat among the major fat depots: IMF, subcutaneous fat(SCF) and visceral fat(VF). In most genotypes of cattle and sheep, the rate of accretion is lower for IMF than for SCF and VF, so genetic selection for a high level of IMF, or the use of an increased dietary energy supply to promote IMF deposition, will increase overall fatness and feed costs. On the other hand, feeding postnatal calves with excessive concentrates promotes IMF deposition, so a nutritional strategy is feasible. With genetic strategies, several problems arise: 1) positive genetic correlations between IMF, SCF and VF differ among genotypes in both cattle and sheep; 2) genotypes appear to have specific, characteristic rates of accretion of IMF during periods of growth and fattening; 3) most breeds of cattle and sheep naturally produce meat with relatively low levels of IMF, but IMF does vary substantially among individuals and breeds so progress is possible through accurate measurement of IMF. Therefore, an essential prerequisite for selection will be knowledge of the genetic correlations and fat accretion rates for each genotype. Currently,selection for IMF is based on existing technology that directly measures IMF in the progeny or siblings, or estimates IMF in live animals. New technology is needed to permit the simultaneous measurement of SCF and IMF in the field, thus opening up the possibility of accurate selection, particularly for fat partitioning in live animals. Specifically, there would be great value in detecting individuals with an IMF advantage at an early age so the generation interval could be shortened and genetic gain accelerated. Genetic gain would also be grea

关键词： Fat depot Fat partition Intramuscular fat Feed efficiency Bovine Ovine

Evaluation of energy values of high-fiber dietary ingredients with different solubility fed to growing pigs using the difference and regression methods

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

animal Nutrition 2021年第2期7卷 569-575页

作者： Zhengqun Liu Ruqing Zhong Kai Li Liang Chen Bifeng Zhang Lei Liu Hongfu Zhang State Key Laboratory of animal Nutrition Institute of Animal ScienceChinese Academy of Agricultural SciencesBeijing100193China institute of animal Science and Veterinary Tianjin Academy of Agricultural SciencesTianjin300381China

The objective of this study was to compare the energy values of high-fiber dietary ingredients with different solubility(sugar beet pulp[SBP]and defatted rice bran[DFRB])in growing pigs using the difference and the regression methods.A total of 21 barrows(initial BW,40.5±1.2 kg)were assigned to 3 blocks with BW as a blocking factor,and each block was assigned to a 7×2 incomplete Latin square design with 7 diets and two 13-d experimental *** 7 experimental diets consisted of a corn-soybean meal basal diet and 6 additional diets containing 10%,20%,or 30%SBP or DFRB in the basal diet,*** of the exper-imental periods lasted 12 d,with a 7 d dietary adaptation period followed by 5-d total fecal and urine *** showed that the digestible energy(DE)and metabolizable energy(ME)of the SBP determined by the difference method with different inclusion levels(10%,20%,or 30%)were 2,712 and 2,628 kcal/kg,2,683 and 2,580 kcal/kg,and 2,643 and 2,554 kcal/kg DM basis,*** DE and ME in the DFRB evaluated by the difference method with 3 different inclusion levels were 2,407 and 2,243 kcal/kg,2,687 and 2,598 kcal/kg,and 2,630 and 2,544 kcal/kg DM basis,*** inclusion levels had no effects on the energy values of each test ingredient estimated by the difference *** DE and ME of the SBP and the DFRB estimated by the regression method were 2,562 and 2,472 kcal/kg and 2,685 and 2,606 kcal/kg DM basis,*** energy values of each ingredient determined by the regression method were similar to the values estimated by the difference method with the 20%or 30%inclusion ***,the energy values of the SBP and DFRB estimated by the difference method with the 10%inclusion level were inconsistent with the values determined by the regression method(P<0.05).In conclusion,the regression method was a robust indirect method to evaluate the energy values for high-fiber ingredients with different solubility in growing pi

关键词： High-fiber ingredient Energy value Difference method Regression method Inclusion level Growing pigs

Hexokinase 1 and 2 mediates glucose utilization to regulate the synthesis of kappa casein via ribosome protein subunit 6 kinase 1 in bovine mammary epithelial cells

在线全文

学校读者我要写书评

暂无评论

animal Nutrition 2024年第1期16卷 338-349页

作者： Tianyu Yang Jia Guo Han Song Osmond Datsomor Yuhang Chen Maocheng Jiang Kang Zhan Guoqi Zhao institute of animal Culture Collection and Application College of Animal Science and TechnologyYangzhou UniversityYangzhou 225009China

Glucose plays a vital part in milk protein synthesis through the mTOR signaling pathway in bovine mammary epithelial cells(BMEC).The objectives of this study were to determine how glucose affects hexokinase(HK)activity in BMEC and investigate the regulatory effect of HK in kappa casein(CSN3)synthesis via the mechanistic target of rapamycin complex 1(mTORC1)signaling pathway in *** this,HK1 and HK2 were knocked out in BMEC using the CRISPR/Cas9 *** gene and protein expression,glucose uptake,and cell proliferation were *** found that glucose uptake,cell proliferation,CSN3 gene expression levels,and expression of HK1 and HK2 increased with increasing glucose ***,glucose uptake was significantly reduced in HK2 knockout(HK2KO)BMEC treated with 17.5 mM ***,under the same glucose treatment conditions,the proliferative ability and abundance of CSN3 were significantly diminished in both HK1 knockout(HK1KO)and HK2KO BMEC compared with that in wild-type *** further observed that the phosphorylation levels of ribosome protein subunit 6 kinase 1(S6K1)were reduced in HK1KO and HK2KO BMEC following treatment with 17.5 mM *** expected,the levels of glucose-6-phosphate and the m RNA expression levels of glycolysis-related genes were decreased in both HK1KO and HK2KO BMEC following glucose *** results indicated that the knockout of HK1 and HK2 inhibited cell proliferation and CSN3 expression in BMEC under glucose treatment,which may be associated with the inactivation of the S6K1 and inhibition of glycolysis.

关键词： Glucose Hexokinase Milk protein Mechanistic target of rapamycin complex 1 signaling pathway Bovine mammary epithelial cell Kappa casein

Effects of the dietary nonfiber carbohydrate content on lactation performance, rumen fermentation, and nitrogen utilization in mid-lactation dairy cows receiving corn stover

在线全文

学校读者我要写书评

暂无评论

Journal of animal Science and Biotechnology 2018年第3期9卷 699-705页

作者： Zihai Wei Baoxin Zhang Jianxin Liu institute of Dairy Science MoE Key Laboratory of Molecular Animal NutritionCollege of Animal Sciences Zhejiang University

Background: Corn stover(CS) is an abundant source of feed for livestock in China. However, it is low in nutritional value that we have been seeking technologies to improve. Previous studies show that non-fiber carbohydrate(NFC)might limit the utilization of a CS diet by lactating dairy cows. Thus, this study was conducted to investigate the lactation performance and rumen fermentation characteristics in lactating cows consuming CS with two contents of NFC compared to an alfalfa hay-containing diet. Twelve Holstein cows were used in a replicated 3 × 3 Latin square design with three dietary treatments:(1) low-NFC diet(NFC = 35.6%, L-NFC),(2) high-NFC diet(NFC = 40.1%,H-NFC), and(3) alfalfa hay diet(NFC = 38.9%, AH).Results: Intake of DM was lower for cows fed H-NFC compared to L-NFC and AH, while the milk yield was higher in AH than in H-NFC and L-NFC(P 0.11), but milk fat content was higher for cows fed H-NFC and L-NFC compared to AH(P < 0.01). The rumen ammonia nitrogen concentration and the concentrations of urea nitrogen in blood and milk were lower for cows fed H-NFC and AH compared to L-NFC(P < 0.05). The concentrations of rumen propionate and total volatile fatty acids were different among groups(P < 0.05) with higher concentration for cows fed AH compared to H-NFC and L-NFC, and acetate concentration tended to be different among groups(P = 0.06).Conclusions: From the results obtained in this study, it was inferred that the increased NFC content in a diet containing corn stover can improve the feed efficiency and benefit the nitrogen conversion.

关键词： Corn stover Dairy cows Lactation performance Nonfiber carbohydrate Rumen fermentation

The effect of dietary tryptophan levels on oxidative stress of liver induced by diquat in weaned piglets

在线全文

学校读者我要写书评

暂无评论

Journal of animal Science and Biotechnology 2015年第1期6卷 104-110页

作者： Xiangbing Mao Mei Lv Bing Yu Jun He Ping Zheng Jie Yu Quyuan Wang Daiwen Chen animal Nutrition institute Sichuan Agricultural University Key Laboratory of animal Disease-Resistance Nutrition Ministry of Education

Oxidative stress can induce abnormal tryptophan metabolism. The present study was mainly conducted to determine the effect of dietary tryptophan levels on oxidative stress in the liver of weaned pigs challenged by diquat. A total of 36 PIC piglets weaned at 21 days of age were randomly allotted to 1 of 3 diets containing dietary tryptophan levels of 0.18, 0.30, and 0A5% for 14 d. On day 8, the piglets were injected intraperitoneally with sterile 0.9% NaCI solution or diquat （10 mg/kg body weight）. During the first 7 d of trial, increasing dietary tryptophan levels enhanced average daily gain （P = 0.09） and average daily feed intake （P = 0.08）, and decreased the feed efficiency （P 〈 0.05） of piglets. The growth performance was decreased by diquat injection （P 〈 0.05）. Diquat injection also decreased the activities of the superoxide dismutase （SOD） and glutathione peroxidase （GPx） in the plasma and liver （P 〈 0.05）, increased plasma malondialdehyde （MDA）（P 〈 0.05） and urea nitrogen （P 〈 0.05） concentrations, and enhanced MDA concentration （P = 0.09） and tryptophan 2,3-dioxygenase （TDO） activity （P = 0.07） in liver of piglets. Increasing dietary tryptophan levels could attenuate the effects of diquat injection on the MDA （P = 0.06） concentration and the activities of SOD （P = 0.09） and GPx （P = 0.05） of the liver, and plasma urea nitrogen （P = 0.06） concentration in the piglet. There was a synergistic role for increasing TDO activity in the liver between dietary tryptophan levels and diquat injection （P 〈 0.05）. These results suggest that increasing dietary tryptophan levels could attenuate the oxidative stress of the liver in weaned piglets intraperitoneally injected with diquat via enhancing the antioxidant capacity.

关键词： Antioxidant capacity Diquat Oxidative stress of livers Tryptophan,Weaned piglets