The effect of dietary tryptophan levels on oxidative stress of liver induced by diquat in weaned piglets

作     者：Xiangbing Mao Mei Lv Bing Yu Jun He Ping Zheng Jie Yu Quyuan Wang Daiwen Chen 

作者机构：Animal Nutrition InstituteSichuan Agricultural University Key Laboratory of Animal Disease-Resistance NutritionMinistry of Education 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2015年第6卷第1期

页      面：104-110页

核心收录：

学科分类：0710[理学-生物学] 090502[农学-动物营养与饲料科学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 0905[农学-畜牧学] 08[工学] 0817[工学-化学工程与技术] 09[农学] 

基　　金：financially supported by the earmarked fund for the China Agriculture Research System(CARS-36) the grant from the Science and Technology Support Program of Sichuan Province(13ZC2237) 

主　　题：Antioxidant capacity Diquat Oxidative stress of livers Tryptophan,Weaned piglets 

摘      要：Oxidative stress can induce abnormal tryptophan metabolism. The present study was mainly conducted to determine the effect of dietary tryptophan levels on oxidative stress in the liver of weaned pigs challenged by diquat. A total of 36 PIC piglets weaned at 21 days of age were randomly allotted to 1 of 3 diets containing dietary tryptophan levels of 0.18, 0.30, and 0A5% for 14 d. On day 8, the piglets were injected intraperitoneally with sterile 0.9% NaCI solution or diquat （10 mg/kg body weight）. During the first 7 d of trial, increasing dietary tryptophan levels enhanced average daily gain （P = 0.09） and average daily feed intake （P = 0.08）, and decreased the feed efficiency （P 〈 0.05） of piglets. The growth performance was decreased by diquat injection （P 〈 0.05）. Diquat injection also decreased the activities of the superoxide dismutase （SOD） and glutathione peroxidase （GPx） in the plasma and liver （P 〈 0.05）, increased plasma malondialdehyde （MDA） （P 〈 0.05） and urea nitrogen （P 〈 0.05） concentrations, and enhanced MDA concentration （P = 0.09） and tryptophan 2,3-dioxygenase （TDO） activity （P = 0.07） in liver of piglets. Increasing dietary tryptophan levels could attenuate the effects of diquat injection on the MDA （P = 0.06） concentration and the activities of SOD （P = 0.09） and GPx （P = 0.05） of the liver, and plasma urea nitrogen （P = 0.06） concentration in the piglet. There was a synergistic role for increasing TDO activity in the liver between dietary tryptophan levels and diquat injection （P 〈 0.05）. These results suggest that increasing dietary tryptophan levels could attenuate the oxidative stress of the liver in weaned piglets intraperitoneally injected with diquat via enhancing the antioxidant capacity.