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Neural Regeneration Research 2025年第1期20卷 199-200页

作者： Srishti Chawla Mikael Molin Thomas Nystrom institute for Biomedicine Sahlgrenska AcademyCentre for Ageing and Health-AgeCapUniversity of GothenburgGothenburgSweden Department of Life Sciences Chalmers University of TechnologyGothenburgSweden

Calcineurin(CN)is a calcium-and calmodulindependent serine/threonine that has been studied in many model organisms including yeast,filamentous fungi,plants,and *** biological functions range from ion homeostasis and virulence in lower eukaryotes to T-cell activation in humans by human nuclear factors of activated *** is a heterodimeric protein consisting of a catalytic subunit,calcineurin A(Cna1p),which contains an active site with a dinuclear metal center,and a regulatory Ca^(2+) binding subunit called calcineurin B(Cnb1p)required to activate *** calcineurin B subunit has been highly conserved through evolution:For example,the mammalian calcineurin B shows 54%identity with calcineurin B from Saccharomyces cerevisiae.

关键词： activation dinuclear conserved

Increased excitatory amino acid transporter 2 levels in basolateral amygdala astrocytes mediate chronic stress–induced anxiety-like behavior

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Neural Regeneration Research 2025年第6期20卷 1721-1734页

作者： Xirong Xu Shoumin Xuan Shuai Chen Dan Liu Qian Xiao Jie Tu Shenzhen Key Laboratory of Neuroimmunomodulation for Neurological diseases Shenzhen-Hong Kong Institute of Brain ScienceShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina CAS Key Laboratory of Brain Connectome and Manipulation Brain Cognition and Brain Disease Institute(BCBDI)Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina Guangdong Provincial Key Laboratory of Brain Connectome and Behavior the Brain Cognition and Brain Disease Institute(BCBDI)Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina University of Chinese of Academy of Sciences BeijingChina Faculty of Life and Health Sciences Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina

The conventional perception of astrocytes as mere supportive cells within the brain has recently been called into question by empirical evidence, which has revealed their active involvement in regulating brain function and encoding behaviors associated with ***, astrocytes in the basolateral amygdala have been found to play a role in the modulation of anxiety-like behaviors triggered by chronic stress. Nevertheless, the precise molecular mechanisms by which basolateral amygdala astrocytes regulate chronic stress–induced anxiety-like behaviors remain to be fully elucidated. In this study, we found that in a mouse model of anxiety triggered by unpredictable chronic mild stress, the expression of excitatory amino acid transporter 2 was upregulated in the basolateral amygdala. Interestingly, our findings indicate that the targeted knockdown of excitatory amino acid transporter 2 specifically within the basolateral amygdala astrocytes was able to rescue the anxiety-like behavior in mice subjected to stress. Furthermore, we found that the overexpression of excitatory amino acid transporter 2 in the basolateral amygdala, whether achieved through intracranial administration of excitatory amino acid transporter 2agonists or through injection of excitatory amino acid transporter 2-overexpressing viruses with GfaABC1D promoters, evoked anxiety-like behavior in mice. Our single-nucleus RNA sequencing analysis further confirmed that chronic stress induced an upregulation of excitatory amino acid transporter 2 specifically in astrocytes in the basolateral amygdala. Moreover, through in vivo calcium signal recordings, we found that the frequency of calcium activity in the basolateral amygdala of mice subjected to chronic stress was higher compared with normal *** knocking down the expression of excitatory amino acid transporter 2 in the basolateral amygdala, the frequency of calcium activity was not significantly increased, and anxiety-like behavior was ob

关键词： anxiety astrocytes basolateral amygdala behavior dihydrokainic acid excitatory amino acid transporter 2 fiber photometry glutamate LDN-212320 transporter

A feeder-free,human plasma-derived hydrogel for maintenance of a human embryonic stem cell phenotype in vitro

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Cell Regeneration 2012年第1期1卷 38-46页

作者： Fiona C Lewis Nicholas Bryan John A Hunt Clinical Engineering UKCTEInstitute of Ageing and Chronic DiseaseUniversity of LiverpoolLiverpool L693GAUK

Background:Human embryonic stem cells(hESCs)represent a tremendous resource for cell therapies and the study of human development;however to maintain their undifferentiated state in vitro they routinely require the use of mouse embryonic fibroblast(MEF)feeder-layers and exogenous protein media ***:These well established requirements can be overcome and in this study,it will be demonstrated that phenotypic stability of hESCs can be maintained using a novel,human plasma protein-based hydrogel as an extracellular culture matrix without the use of feeder cell *** were resuspended in human platelet poor plasma(PPP),which was gelled by the addition of calcium containing DMEM-based hESC culture *** and genomic expression of the pluripotency markers OCT4,NANOG and SOX2 were measured using immunohistochemistry and qRT-PCR *** hESC morphology was demonstrated throughout in vitro culture and both viability and phenotypic stability were maintained throughout extended culture,up to 25 ***:PPP-derived hydrogel has demonstrated to be an efficacious alternative to MEF co-culture with its hydrophilicity allowing for this substrate to be delivered via minimally invasive procedures in a liquid phase with polymerization ensuing in *** this provides a novel technique for the study of this unique group of stem cells in either 2D or 3D both in vitro and in vivo.

关键词： Embryonic stem cells Hydrogel Pluripotent stem cells Cell culture

Biofilms and Helicobacter pylori: Dissemination and persistence within the environment and host

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World Journal of Gastrointestinal Pathophysiology 2014年第3期5卷 122-132页

作者： Steven L Percival Louise Suleman Surface Science Research Centre and institute of ageing and chronic disease University of Liverpool Musculoskeletal Biology Institute of Ageing and Chronic Disease University of Liverpool

The presence of viable Helicobacter pylori(H. pylori) in the environment is considered to contribute to the levels of H. pylori found in the human population, which also aids to increase its genetic variability and its environment adaptability and persistence. H. pylori form biofilms both within the in vitro and in vivo envi-ronment. This represents an important attribute that assists the survival of this bacterium within environ-ments that are both hostile and adverse to prolifera-tion. It is the aim of this paper to review the ability of H. pylori to form biofilms in vivo and in vitro and to address the inherent mechanisms considered to sig-nificantly enhance its persistence within the host and in external environments. Furthermore, the dissemi-nation of H. pylori in the external environment and within in the human body and its impact upon infec-tion control shall be discussed.

关键词： Helicobacter pylori Biofilm Coccoid forms Virulence Water

Standardization of choroidal thickness measurements using enhanced depth imaging optical coherence tomography

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International Journal of Ophthalmology(English edition) 2015年第3期8卷 484-491页

作者： Nattapon Boonarpha Yalin Zheng Alexandros N.Stangos Huiqi Lu Ankur Raj Gabriela Czanner Simon P.Harding Jayashree Nair-Sahni Department of Eye and Vision Science Institute of Ageing and Chronic Disease University of Liverpool St.Paul's Eye Unit Royal Liverpool University Hospital Department of Biostatistics University of Liverpool

AIM: To describe and evaluate a standardized protocol for measuring the choroidal thickness(Ch T) using enhanced depth imaging optical coherence tomography(EDI OCT).METHODS: Single 9 mm EDI OCT line scans across the fovea were used for this study. The protocol used in this study classified the EDI OCT images into four groups based on the appearance of the choroidal-scleral interface and suprachoroidal space. Two evaluation iterations of experiments were performed: first, the protocol was validated in a pilot study of 12 healthy eyes. Afterwards, the applicability of the protocol was tested in 82 eyes of patients with diabetes. Inter-observer and intra-observer agreements on image classifications were performed using Cohen’s kappa coefficient(κ). Intraclass correlation coefficient(ICC) and Bland-Altman’s methodology were used for the measurement of the Ch ***: There was a moderate(κ=0.42) and perfect(κ =1) inter- and intra-observer agreements on image classifications from healthy eyes images and substantial(κ =0.66) and almost perfect(κ =0.86) agreements from diabetic eyes images. The proposed protocol showed excellent inter- and intra-observer agreements for the Ch T measurements on both, healthy eyes and diabetic eyes(ICC >0.90 in all image categories). The Bland-Altman plot showed a relatively large Ch T measurement agreement in the scans that contained less visible choroidal outer boundary. CONCLUSION: A protocol to standardize Ch T measurements in EDI OCT images has been developed;the results obtained using this protocol show that the technique is accurate and reliable for routine clinical practice and research.

关键词： choroid enhanced depth imaging choroidal thickness optical coherence tomography diabetes

Characteristics of Poisoning Cases Admitted to Outpatient and Emergency Department—China,2019

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China CDC weekly 2023年第47期5卷 1052-1057页

作者： Xiao Deng Ye Jin Yuan Wang Pengpeng Ye Yuan Yuan Chengye Sun Leilei Duan National institute for Occupational Health and Poison Control Chinese Center for Disease Control and PreventionBeijingChina National Center for chronic and Noncommunicable disease Control and Prevention Chinese Center for Disease Control and PreventionBeijingChina

Summary What is already known about this topic?Poisoning constitutes a significant cause of mortality among individuals aged 1 to 44 in ***,the epidemiological understanding of poisoning incidents within emergency dep... 详细信息

关键词： Poisoning mortality Cases

Breast Cancer Screening Coverage—China,2018-2019

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China CDC weekly 2023年第15期5卷 321-326页

作者： Mei Zhang Heling Bao Xiao Zhang Zhengjing Huang Zhenping Zhao Chun Li Maigeng Zhou Jing Wu Limin Wang Linhong Wang Division of NCD and Risk Factor Surveillance National Center for Chronic and Noncommunicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijingChina institute of Medical Information Chinese Academy of Medical Sciences&Peking Union Medical CollegeBeijingChina National Center for chronic and Noncommunicable disease Control and Prevention China CDCBeijingChina

Summary What is already known about this topic?In 2015,only 18.9%of adult women underwent breast cancer screening in *** is added by this report?Breast cancer screening coverage for women aged 20 years and above in China reached 22.3%during *** with lower socioeconomic status had lower screening *** were significant variations across the provincial-level administrative divisions.

关键词： Breast Cancer cancer

Cervical Cancer Screening Rates Among Chinese Women—China,2015

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China CDC weekly 2020年第26期2卷 481-486页

作者： Mei Zhang Yijing Zhong Zhenping Zhao Zhengjing Huang Xiao Zhang Chun Li Maigeng Zhou Jing Wu Limin Wang Xiaoying Zheng Linhong Wang National Center for chronic and Non-Communicable disease Control and Prevention Chinese Center for Disease Control and PreventionBeijingChina institute of Population Research Peking UniversityBeijingChina

What is already known about this topic?Cervical cancer is the fourth most common cancer of women around the ***-adjusted incidence and mortality rates of cervical cancer were 11.78 and 3.29 per 100,000,respectively,in China in *** is added by this report?Cervical cancer screening rates were 25.7%for women aged 20–64 years old and 31.4%for women aged 35–64 years old in China in *** rates were lower in rural areas than in urban areas and varied across *** are the implications for public health practice?Efforts should be made to continue to strengthen national and local policy initiatives,financial support,health education,and accessibility to women in rural areas for cervical cancer screening coverage.

关键词： Cervical education policy

Interconnections between diabetic corneal neuropathy and diabetic retinopathy:diagnostic and therapeutic implications

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Neural Regeneration Research 2025年第8期20卷 2169-2180页

作者： Mingyi Yu Faith Teo En Ning Chang Liu Yu-Chi Liu Tissue Engineering and Stem Cell Group Singapore Eye Research InstituteSingapore Yong Loo Lin School of Medicine National University of SingaporeSingapore Department of Cornea and External Eye disease Singapore National Eye CenterSingapore Ophthalmology and Visual Sciences Academic Clinical Program Duke-NUS Medical SchoolSingapore

Diabetic corneal neuropathy and diabetic retinopathy are ocular complications occurring in the context of diabetes *** corneal neuropathy refers to the progressive damage of corneal *** retinopathy has traditionally been considered as damage to the retinal ***,growing evidence suggests that diabetic retinopathy is a complex neurovascular disorder resulting from dysfunction of the neurovascular unit,which includes both the retinal vascular structures and neural *** retinopathy is one of the leading causes of blindness and is frequently screened for as part of diabetic ocular ***,diabetic corneal neuropathy is commonly overlooked and underdiagnosed,leading to severe ocular surface *** studies have found that these two conditions tend to occur together,and they share similarities in their pathogenesis pathways,being triggered by a status of chronic *** review aims to discuss the interconnection between diabetic corneal neuropathy and diabetic retinopathy,whether diabetic corneal neuropathy precedes diabetic retinopathy,as well as the relation between the stage of diabetic retinopathy and the severity of corneal *** also endeavor to explore the relevance of a corneal screening in diabetic eyes and the possibility of using corneal nerve measurements to monitor the progression of diabetic retinopathy.

关键词： cornea corneal nerves diabetes diabetic corneal neuropathy diabetic retinopathy retina

Trends and Regional Distribution Pattern of Female Lung Cancer--China,1992-2016

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China CDC weekly 2020年第32期2卷 614-618页

作者： Xue Ni Bei Zhang Ning Xu Xinying Zeng Qiang Wang National institute of Environmental Health Chinese Center for Disease Control and PreventionBeijingChina The National Center for chronic and Noncommunicable disease Control and Prevention Chinese Center for Disease Control and PreventionBeijingChina

What is already known about this topic?The incidence of female lung cancer in China has been rising,whereas the incidence ratio among men and women is *** rising trend of female lung cancer is a prominent public health concern for *** is added by this report?The joint secular trends distribution of the incidence,mortality,and years of life lost(YLLs)due to premature death of lung cancer in Chinese women demonstrated a certain regionality,which implied the risk factors for female lung cancer in China might be different by *** determinant risk factors may exist in regions sharing the same joint secular *** are the implications for public health practice?The secular trends and the regional pattern of the trends of female lung cancer in China suggested that the prevention and control of female lung cancer should be implemented with targeted regional interventions.

关键词： lung prevention cancer