A feeder-free,human plasma-derived hydrogel for maintenance of a human embryonic stem cell phenotype in vitro

作     者：Fiona C Lewis Nicholas Bryan John A Hunt 

作者机构：Clinical EngineeringUKCTEInstitute of Ageing and Chronic DiseaseUniversity of LiverpoolLiverpool L693GAUK 

出 版 物：《Cell Regeneration》 (细胞再生（英文）)

年 卷 期：2012年第1卷第1期

页      面：38-46页

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100101[医学-人体解剖与组织胚胎学] 10[医学] 

基　　金：Funding from the EPSRC for this study is gratefully acknowledged 

主　　题：Embryonic stem cells Hydrogel Pluripotent stem cells Cell culture 

摘      要：Background:Human embryonic stem cells(hESCs)represent a tremendous resource for cell therapies and the study of human development;however to maintain their undifferentiated state in vitro they routinely require the use of mouse embryonic fibroblast(MEF)feeder-layers and exogenous protein media ***:These well established requirements can be overcome and in this study,it will be demonstrated that phenotypic stability of hESCs can be maintained using a novel,human plasma protein-based hydrogel as an extracellular culture matrix without the use of feeder cell *** were resuspended in human platelet poor plasma(PPP),which was gelled by the addition of calcium containing DMEM-based hESC culture *** and genomic expression of the pluripotency markers OCT4,NANOG and SOX2 were measured using immunohistochemistry and qRT-PCR *** hESC morphology was demonstrated throughout in vitro culture and both viability and phenotypic stability were maintained throughout extended culture,up to 25 ***:PPP-derived hydrogel has demonstrated to be an efficacious alternative to MEF co-culture with its hydrophilicity allowing for this substrate to be delivered via minimally invasive procedures in a liquid phase with polymerization ensuing in *** this provides a novel technique for the study of this unique group of stem cells in either 2D or 3D both in vitro and in vivo.