检索结果-南通市图书馆

The Crop Journal 2022年第3期10卷 638-646页

作者： Wen Chu Jia Liu Hongtao Cheng Chao Li Li Fu Wenxiang Wang Hui Wang Mengyu Hao Desheng Mei Kede Liu Qiong Hu oil crops Research institute of Chinese Academy of Agricultural Sciences/Key Laboratory for Biology and Genetic Improvement of oil crops Ministry of Agriculture and Rural AffairsWuhan 430062HubeiChina National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070HubeiChina Graduate School of Chinese Academy of Agricultural Sciences Beijing 100081China

Pod shattering causes severe yield loss in rapeseed(Brassica napus L.)under modern agricultural *** of highly shatter-resistant germplasm is desirable for the development of rapeseed cultivars for mechanical *** the present study,an elite line OR88 with strong shatter resistance and a lignified-layer bridge(LLB)structure was *** LLB structure was unique to OR88 and co-segregated with high pod-shatter *** LLB structure is differentiated at stage 12 of gynoecium development without any gynoecium *** analysis showed that LLB is controlled by a single recessive *** BSA-Seq and map-based cloning,the resistance gene location was delimited to a0.688 Mb region on chromosome *** analysis suggested Bn TCP8.C09 as the gene responsible for *** expression of Bn TCP.C09 was strongly downregulated in OR88,suppressing cell proliferation in the pod valve *** markers linked to the candidate gene were *** pod shatter-resistant line could be used in rapeseed breeding programs by direct transfer of the gene with the assistance of the DNA markers.

关键词： Pod shatter Lignified-layer bridge BSA-Seq KASP marker Rapeseed

Editing of Rice Isoamylase Gene ISA1 Provides Insights into Its Function in Starch Formation

在线全文

学校读者我要写书评

暂无评论

Rice science 2019年第2期26卷 77-87页

作者： CHAO Shufen CAI Yicong FENG Baobing JIAO Guiai SHENG Zhonghua LUO Ju TANG Shaoqing WANG Jianlong HU Peisong WEI Xiangjin State Key Laboratory of Rice Biology China National Rice Research Institute Southern Regional Collaborative Innovation Center for Grain and oil crops College of Agronomy Hunan Agricultural University

Isoamylase 1(ISA1) is an isoamylase-type debranching enzyme which plays a predominant role in amylopectin synthesis. In this study, the clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated endonuclease 9(CRISPR/Cas9) system was used to edit ISA1 gene in rice via Agrobacterium-mediated transformation. We identified 36 genetic edited lines from 55 T_0 transgenic events, and classified the mutation forms into 7 types. Of those, two homozygous mutants, cr-isa1-1(type 1, with an adenine insertion) and cr-isa1-2(type 3, with a cytosine deletion) were selected for further analysis. Seed sizes of both cr-isa1-1 and cr-isa1-2 were affected, and the two mutants also displayed a shrunken endosperm with significantly lower grain weight. Electron microscopy analysis showed that abnormal starch granules and amyloplasts were found in cr-isa1-1 and cr-isa1-2 endosperm cells. The contents of total starch, amylose and amylopectin in the endosperm of the cr-isa1 mutants were significantly reduced, whereas sugar content and starch gel consistency were observably increased compared to the wild-type. The gelatinization temperature and starch chain length distributions of the cr-isa1 mutants were also altered. Moreover, transcript levels of most starch synthesis-related genes were significantly lower in cr-isa1 mutants. In conclusion, the results indicated that gene edition of ISA1 affected starch synthesis and endosperm development, and brought potential implications for rice quality breeding.

关键词： ISA1 rice starch biosynthesis starch granule physicochemical property CRISPR Cas9

Genome-Wide Identification of Zn_(2)Cys_(6 ) Class Fungal-Specific Transcription Factors(ZnFTFs)and Functional Analysis of UvZnFTFI in Ustilaginoidea virens

在线全文

学校读者我要写书评

暂无评论

Rice science 2021年第6期28卷 567-578,I0038,I0039页

作者： SONG Tianqiao ZHANG Xiong ZHANG You LIANG Dong YAN Jiaoling YU Junjie YU Mina CAO Huijuan YONG Mingli PAN Xiayan QI Zhongqiang DU Yan ZHANG Rongsheng LIU Yongfeng institute of Plant Protection Jiangsu Academy of Agricultural SciencesNanjing 210014China School of Life Sciences Jiangsu UniversityZhenjiang 212013China Key Laboratory of Biology and Genetic Improvement of oil crops Ministry of Agriculture and Rural Affairs of China/Oil Crops Research InstituteChinese Academy of Agricultural SciencesWuhan 430062China School of the Environment and Safety Engineering Jiangsu UniversityZhenjiang 212013China

Transcription factors(TFs)orchestrate the regulation of cellular gene expression and thereby determine cell *** this study,we analyzed the distribution of TFs containing domains,which named as ZnFTFs,both in ascomycete and basidiomycete *** found that ZnFTFs were widely distributed in these fungal species,but there was more expansion of the ZnFTF class in Ascomycota than *** identified 40 ZnFTFs in Ustilaginoidea virens,and demonstrated the involvement of UvZnFTF1 in vegetative growth,conidiation,pigment biosynthesis and ***-Seq analysis suggested that UvZnFTF1 may regulate different nutrient metabolism pathways,the production of secondary metabolites,and the expression of pathogen-host interaction genes and secreted protein-encodi ng *** of the distributi on of differe nt fungal TFs in *** further dem on strated that UvZnFTFs make up a large TF family and may play essential biological roles in ***.

关键词： Zn_(2)Cys_(6)-type fungal-specific transcription factor Ustilaginoidea virens rice pathogen gene silencing RNA-Seq metabolism pathway effector expression pathogen-host interaction

Characterization of Genomic Integration and Transgene Organization in Six Transgenic Rapeseed Events

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2014年第9期13卷 1865-1876页

作者： WU Yu-hua ZHANG Li WU Gang NIE Shu-jing LU Chang-ming oilcrops Research institute Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil CropsMinistry of Agriculture College of Life Science South-Central University for Nationalities

To characterize the DNA rearrangement of both the T-DNA region and the genomic insertion site during T-DNA insertion, the Genomewalker strategy was used to isolate the junctions between the inserted DNA and the plant genomic DNA in six rapeseed events as well as the genomic DNA at the sites before integration. During transformation in each of the six events, portions of both the right border（RB） and left border（LB） regions of the T-DNA were deleted, ranging from a 7 nucleotide deletion of the LB repeats in event RF1 to a 207 bp deletion of the LB region in event RF2. For the six events, T-DNA integration resulted in a deletion at the target site spanning less than 100 bp. Sequence analysis indicated that the T-DNA was integrated into the coding region of various native rapeseed genes in events RF1 and RF2. Duplications of the genomic DNA target site were observed in events RF2, RF3 and Topas 19/2. And multimerization of transgenes was found in event Topas 19/2, in which, the T-DNA was integrated as a head-to-head（RB-to-RB） concatemer into the recipient genome. In event MS1, chromosomal translocation or a large target-site deletion may have occurred during T-DNA integration, which was identified due to a failure to amplify the presumptive insertion site based on the flanking rapeseed DNA sequences. Our results provide comprehensive data concerning transgene organization and the genomic context of the T-DNA in six rapeseed events, which can aid in the developing of insert fingerprinting and the monitoring of long-term genetic stability and potential unintended effects of transgenic events.

关键词： transgenic rapeseed junction fragment pre-insertion site DNA rearrangement

Mechanical Harvesting Effects on Seed Yield Loss,Quality Traits and Profitability of Winter oilseed Rape (Brassica napus L.)

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2012年第8期11卷 1297-1304页

作者： MA Ni ZHANG Chun-lei LI Jun ZHANG Ming-hai CHENG Yu-gui LI Guang-ming ZHANG Shu-jie oil crops Research institute Chinese Academy of Agricultural Sciences/Key Laboratory of Oil Crop Biology Ministry of Agriculture/Key Laboratory of Crop Cultivation and Physiology Ministry of Agriculture Wuhan 430062 PR.China Agricultural institute of Yichang City Yichang 443004 P.R.China

China is one of the most important rapeseed producing countries in the world. Effective mechanical harvesting time for decreasing harvesting loss of winter oilseed rape has been becoming a critical factor. An elite cultivar Zhongshuang 11 （Brassica napus L.） was employed in two rounds of field experiments from 2009 to 2011. Seeds were sown with machine, three combine harvesting times namely combine harvesting A, B, and C （CHA, CHB, and CHC） were designed and manual harvesting （MH） as control was performed at maturity. The harvesting treatments were determined according to color of pod and seed in the field. Seed yield loss and quality in different treatments were evaluated. Results showed that both seed yields and harvesting losses in 2009-2010 were higher than that in 2010-2011, whereas seed oil contents in 2010-2011 were higher than that in 2009-2010. The highest yield appeared in CHB, which was significantly higher than that in MH. Furthermore, harvesting loss in CHB were 50% that in MH. Seed oil content and chlorophyll exhibited no obvious difference between CHB and MH. Economic profit analysis demonstrated that mechanical sowing/combine harvesting （MS/CH） showed an input/output ratio of 1：1.6, and it was 1：1.2 in mechanical sowing/manual harvesting （MS/MH）. Labor-cost accounted for more than 70% of the total cost in MS/MH, which led to low profitability to a great extent. Our results suggested that CHB was the optimum harvesting time for winter oilseed rape along the Yangtze River.

关键词： winter oilseed rape (Brassica napus L.) combine harvesting harvesting loss seed quality economic profit

Comparison of CWSI and T_(s)-T_(a)-VIs in moisture monitoring of dryland crops(sorghum and maize)based on UAV remote sensing

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2024年第7期23卷 2458-2475页

作者： Hui Chen Hongxing Chen Song Zhang Shengxi Chen Fulang Cen Quanzhi Zhao Xiaoyun Huang Tengbing He Zhenran Gao College of Agriculture Guizhou UniversityGuiyang 550025China institute of New Rural Development Guizhou UniversityGuiyang 550025China institute of Rice Industry Technology Research College of Agricultural SciencesGuizhou UniversityGuiyang 550025China

Monitoring agricultural drought using remote sensing data is crucial for precision irrigation in modern *** unmanned aerial vehicle(UAV)remote sensing,we explored the applicability of an empirical crop water stress index(CWSI)based on canopy temperature and three-dimensional drought indices(TDDI)constructed from surface temperature(T_(s)),air temperature(T_(a))and five vegetation indices(VIs)for monitoring the moisture status of dryland *** machine learning algorithms(random forest regression(RFR),support vector regression,and partial least squares regression)were used to compare the performance of the drought indices for vegetation moisture content(VMC)estimation in sorghum and *** main results of the study were as follows:(1)Comparative analysis of the drought indices revealed that T_(s)-T_(a)-normalized difference vegetation index(TDDIn)and T_(s)-T_(a)-enhanced vegetation index(TDDIe)were more strongly correlated with VMC compared with the other *** indices exhibited varying sensitivities to VMC under different irrigation regimes;the strongest correlation observed was for the TDDIe index with maize under the fully irrigated treatment(r=-0.93).(2)Regarding spatial and temporal characteristics,the TDDIn,TDDIe and CWSI indices showed minimal differences Over the experimental period,with coefficients of variation were 0.25,0.18 and 0.24,*** three indices were capable of effectively characterizing the moisture distribution in dryland maize and sorghum crops,but the TDDI indices more accurately monitored the spatial distribution of crop moisture after a rainfall or irrigation event.(3)For prediction of the moisture content of single crops,RFR models based on TDDIn and TDDIe estimated VMC most accurately(R^(2)>0.7),and the TDDIn-based model predicted VMC with the highest accuracy when considering multiple-crop samples,with R^(2)and RMSE of 0.62 and 14.26%,***,TDDI proved more effective than the CWSI in e

关键词： maize sorghum T_(s)-T_(a)-VIs CWSI UAV machine learning crop moisture monitoring

Combining Ability and Genetic Effects of Germination Traits of Brassica napus *** Waterlogging Stress Condition

在线全文

学校读者我要写书评

暂无评论

Agricultural Sciences in China 2010年第7期9卷 951-957页

作者： CHENG Yong GU Min CONG Ye ZOU Chong-shun ZHANG Xue-kun WANG Han-zhong oil crops Research institute Chinese Academy of Agriculture Sciences/Key Laboratory for Oil Crop Biology Ministry of Agriculture Wuhan 430062. P.R.China Agricultural College Yangtze University Jingzhou 434023 P.R. China

Cross combinations from six rapeseed cultivars and lines were evaluated under waterlogging stress condition in order to understand the genetic mechanism of waterlogging tolerance of Brassica napus *** provide reasonable improvement *** were six germination traits investigated on combining ability and heritability using complete diallel crossing method designed for 30 combinations from those six cultivars and *** traits included relative root length,stem length,fresh weight per plant,survival rate,electrical conductivity,and vigor *** flooding treatment,the six traits of parents and F1 were *** general combining ability（GCA） and special combining ability（SCA） of germination traits were analyzed using Griffing I *** 30 cross combinations,the GCA was significantly different among six waterlogging resistance *** SCA of these traits was significantly different except the SCA of electrical *** a representative trait of waterlogging tolerance in rapeseed,relative vigor index had the highest narrow heritability and relatively low broad *** cultivars Zhongshuang 9 and P79 had higher tolerance potential to waterlogging *** can be concluded that combining ability and genetic effects of relative vigor index during germination stage could be used to identify the waterlogging tolerance of rapeseed in breeding program.

关键词： Brassica napus L. relative vigor index waterlogging tolerance germination trait combining ability diallel crossing

Characterization of a new hexaploid triticale 6D(6A) substitution line with increased grain weight and decreased spikelet number

在线全文

学校读者我要写书评

暂无评论

The Crop Journal 2019年第5期7卷 598-607页

作者： Zhiyu Feng Zhongqi Qi Dejie Du Mingyi Zhang Aiju Zhao Zhaorong Hu Mingming Xin Yingyin Yao Huiru Peng Qixin Sun Zhongfu Ni Key Laboratory of Crop Heterosis and Utilization State Key Laboratory for AgrobiotechnologyBeijing Key Laboratory of Crop Genetic ImprovementCollege of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China institute of Wheat Research Shanxi Academy of Agricultural SciencesLinfen 041000ShanxiChina Hebei Crop Genetic Breeding Laboratory Institute of Cereal and Oil CropsHebei Academy of Agriculture and Forestry SciencesShijiazhuang 050035HebeiChina

Hexaploid triticale(×Triticosecale,AABBRR)is an important forage crop and a promising energy *** D-genome chromosomes or segments from common wheat(Triticum aestivum)into hexaploid triticale is attractive in improving its economically important ***,a hexaploid triticale 6D(6A)substitution line Lin 456 derived from the cross between the octoploid triticale line H400 and the hexaploid wheat Lin 56 was identified and analyzed by genomic in situ hybridization(GISH),fluorescence in situ hybridization(FISH),and molecular *** GISH analysis showed that Lin 456 is a hexaploid triticalewith 14 rye(Secale cereale)chromosomes and 28 wheat chromosomes,whereas non-denaturing fluorescence in situ hybridization(ND-FISH)and molecular marker analysis revealed that it is a 6D(6A)substitution *** contrast to previous studies,the signal of Oligo-pSc119.2 was observed at the distal end of 6DL in Lin *** wheat chromosome 6D was associatedwith increased grain weight and decreased spikelet number using the genotypic data combined with the phenotypes of the F2 population in the three *** thousand-grain weight and grain width in the substitution individuals were significantly higher than those in the non-substitution individuals in the F2 population across the three *** propose that the hexaploid triticale 6D(6A)substitution line Lin 456 can be a valuable and promising donor stock for genetic improvement during triticale breeding.

关键词： In situ hybridization Spikelet number Substitution line Thousand-grain weight Triticale

Progress on genetic and genomic research for enhanced oil content and quality in peanut

在线全文

学校读者我要写书评

暂无评论

oil Crop Science 2016年第1期1卷 1-8页

作者： Boshou LIAO Yong LEI Li HUANG Silong CHEN Xiaoping REN Liying YAN Yurong LI Dongxin HUAI Xiaojing ZHOU Liyun WAN Yuning CHEN Wei HUA Huifang JIANG oil crops Research institute of Chinese Academy of Agricultural Sciences Wuhan 430062 P. R. China cereal and oil crops Research institute of Hebei Academy of Agricultural and Forestry Sciences Shijiazhuang 050035 P. R. China

Peanut or groundnut （ Arachis hypogaea L. ） is an important source of vegetable oil in the world. Genetic enhancement for high yield and high oil content has greatly contributed to enhanced pro-ductivity of peanut and increased supply of peanut oil. Further improving oil content and quality of peanut is still crucial for increasing productivity of arable land and market competitiveness of peanut oil. Based on investigation among the peanut germplasm accessions including wild Arachis species, the oil content could be as high as 65%. Heterosis has been observed for oil content in hybrids derived from diverse crossing parents. Segregates with enhanced oil content have been obtained by pyramiding different genes or alleles with major and minor additive effects. Improved testing techniques for oil content in breeding lines with reduced cost have accelerated breeding progress for high oil content. SSR markers associated with oil content have been identified by association and linkage analysis. The stability of oil content in peanut across seasons and locations is highly associated with cold tolerance, high nutrition efficiency and drought tolerance. Recent progress on improving fatty acids in particular marker assisted backcrossing breeding has contributed to higher quality peanut oil and other products. High oil peanut lines with im-proved resistance to aflatoxin production have been developed. The wild Arachis species would be of great value for peanut breeding in increasing oil content.

关键词： s: Arachis hypogaea L. wild Arachis species oil content oleic acid fatty acid compo-nents resistance to aflatoxin production heterosis marker assisted breeding

A comprehensive review of bioactive compounds and processing technology of sesame seed

在线全文

学校读者我要写书评

暂无评论

oil Crop Science 2022年第2期7卷 88-94页

作者： Xuan Ma Zhi Wang Chang Zheng Changsheng Liu oil crops Research institute of the Chinese Academy of Agricultural Sciences Oil Crops and Lipids Process Technology National&Local Joint Engineering LaboratoryKey Laboratory of Oilseeds ProcessingMinistry of Agriculture and Rural AffairsHubei Key Laboratory of Lipid Chemistry and NutritionWuhan430062China

Sesame seeds are promulgated as traditional high-quality edible oil crops,rich in lipid(40–65%),protein(19–35%),and bioactive *** review starts with bioactive components(fatty acid,tocopherol,phytosterol,sesamin,sesamolin,and sesamol)of sesame *** considers processing techniques for extracting oil(aqueous extraction and pressing)from *** technologies,such as enzyme-assisted aqueous,supercritical CO_(2),and microwave-assisted solvent extraction,are also *** methods of utilization of sesame seed cake are also *** the future,the processing technology of sesame seed will be further developed in the direction of improving comprehensive utilization rate to meet new consumption demand.

关键词： Sesame seed Bioactive compound Nutritional properties Processing technology By-product