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Serum Metabolomic Indicates Potential Biomarkers and Metabolic Pathways of Pediatric Kashin-Beck Disease

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Biomedical and Environmental Sciences 2020年第10期33卷 750-759页

作者： WANG Yu Meng WANG Wei Yi SUN Li Yan LI Qiang ZHAO Zhi Jun HU Jian ZHANG Rong Yu WANG Li Hua Center for endemic Disease Control Chinese Center for Disease Control and PreventionHarbin Medical University/Key Lab of Etiology and EpidemiologyEducation Bureau of Heilongjiang Province&Ministry of HealthHarbin 150081Heilongjiang China Qinghai institute for endemic Disease prevention and Control Xining 811602QinghaiChina

Objective To explore potential serum biomarkers of children with Kashin-Beck Disease(KBD)and the metabolic pathways to which the biomarkers *** A two-stage metabolomic study was *** discovery cohort included 56 patients,51 internal controls,and 50 external *** metabolites were determined by HPLC-(Q-TOF)-MS and confirmed by Human Metabolome Databases(HMDB)and Metlin *** 3.0 and the Kyoto Encyclopedia of Genes and Genomes(KEGG)database were used to analyze the metabolic pathways of the candidate *** use of HPLC-(Q-TRAP)-MS enabled quantitative detection of the target metabolites which were chosen using the discovery study and verified in another independent verification cohort of 31 patients,41 internal controls,and 50 external *** Eight candidate metabolites were identified out in the discovery study,namely kynurenic acid,N-α-acetylarginine,6-hydroxymelatonin,sphinganine,ceramide,sphingosine-1 P,spermidine,and *** metabolites exist in sphingolipid,glutathione,and tryptophan metabolic *** the second-stage study,five candidate metabolites were validated,including kynurenic acid,N-α-acetylarginine,sphinganine,spermidine,and sphingosine-1 *** for spermidine,all substances exhibited low expression in the case group compared with the external control group,and the difference in levels of sphinganine,spermidine,and sphingosine-1 P was statistically *** The direction of change of levels of sphinganine,spermidine,and sphingosine-1 P in the two-stage study cohorts was completely consistent,and the differences were statistically ***,these substances can be used as potential biomarkers of ***,these results raise the possibility that sphingolipid metabolic pathways may be closely related to KBD.

关键词： Kashin-Beck disease Potential biomarkers Metabolism pathways Serum

THE Se-RICH CARBONACEOUS SILICEOUS ROCK AND endemic SELENOSIS IN SOUTHWEST HUBEI,CHINA

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Chinese Science Bulletin 1992年第20期37卷 1725-1729页

作者：郑宝山洪业汤赵伟周怀阳夏卫平苏宏灿毛大钧严良荣 LAIN THORNTONA institute of Geochemistry Academia Siniea Guiyang 550002 PRC The Second institute of Oceanography NOB Hangzhou 310012 PRC endemic diseases Prevention Office of Exi Prefecture Enshi 445000 PRC Department of Geology Imperial College of Science Technology and Medicine London UK.

Se-rich carbonaceous siliceous rocks and carbonaceous shales (known locally as 'stone coal' ) are widespread in the southwest part of Hubei Province, China, where human and livestock selenosis is reported. It has been shown that the selenium contained in soil, drinking water, crops and vegetables all originated directly or indirectly from the seleniferous strata.

关键词： Se rock soil selenosis

Infection and Genomic Characteristics of Campylobacter jejuni from a Patient Without Diarrhea—China,2018

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China CDC weekly 2024年第33期6卷 846-851,I0001-I0005页

作者： Xiangdong Yang Wen Wang Chajin Cui Binbin Yu Qing Zhang Yanhua Wang Ecological Medicine Research Center National Key Laboratory of Intelligent Tracking and Forecasting for Infectious DiseasesNational Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijingChina Department of Zoonotic Disease Control and Prevention Yunnan Institute of Endemic Diseases Control&PreventionDali Bai Autonomous PrefectureYunnan ProvinceChina Medical Laboratory Department Yunnan Luxi County People’s HospitalHonghe Hani-Yi Autonomous PrefectureYunnan ProvinceChina

What is already known about this topic?A 20-month-old boy was admitted to the hospital with a maximum temperature of 40°C and a single ***,blood culture detected Francisella tularensis(***)using the VITEK 2 Compact *** is added by this report?After incubation of the patient’s blood for 48 hours,the cultured strain was identified as Campylobacter jejuni,named L8,excluding *** the genome sequence of L8,we found a novel Type VI Secretion System(T6SS),of which the conserved C-terminal VgrG domain from positions 561 to 884 showed significant *** are the implications for public health practice?It should be underscored that relying solely on automatic bacterial identification instruments for accurate strain identification is ***,our study suggests that the potential effect of T6SS should be considered when studying the genetic features of a patient’s clinical phenotypes.

关键词： Campylobacter admitted culture

Reducing Canine Echinococcus Infection with Smart Deworming Collars--Tibet,China,June-November,2020

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China CDC weekly 2020年第51期2卷 979-982页

作者： Shijie Yang Ning Xiao Jingzhong Li Gongsang Quzhen Junying Ma Qing Yu Zhaohui Luo Huasheng Pang Danzeng Quzhen Suolang Wangjie Xiao-nong Zhou National institute of Parasitic diseases Chinese Center for Disease Control and Prevention/Key Laboratory of Parasite and Vector BiologyMinistry of Health/National Center for International Research on Tropical Diseases/WHO Collaborating Centre for Tropical DiseasesShanghaiChina One Health Center School of Global HealthChinese Center for Tropical Diseases ResearchShanghai Jiao Tong University School of MedicineShanghaiChina Tibet Center for Disease Control and Prevention NHC Key Laboratory of Echinococcosis Prevention and ControlLhasaTibetChina Qinghai institute for endemic Disease Prevention and Control XiningQinghaiChina

What is already known about this topic?Existing manual deworming programs launched have made great progress in reducing the Echinococcus infection rate of domestic dogs,but significant challenges remain in scattered nomadic communities inhabiting the Tibetan *** low deworming frequency and low levels of coverage were responsible for the high infection rate of Echinococcus *** *** is added by this report?Smart deworming collars controlled by a remote management system(RMS)was found to increase the deworming frequency and coverage and subsequently reduce the canine infection rates with Echinococcus spp..What are the implications for public health practice?As an innovative tool,smart deworming collars may drive the paradigm shift from manual deworming to smart deworming and stop the transmission of echinococcosis.

关键词： Echinococcus Smart scattered

Hepatitis D infection:from initial discovery to current investigational therapies

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Gastroenterology Report 2019年第4期7卷 231-245,I0001页

作者： Ben L.Da Theo Heller Christopher Koh Digestive diseases Branch National Institute of Diabetes&Digestive&Kidney DiseasesNational Institutes of HealthBethesdaMDUSA Liver diseases Branch National Institute of Diabetes&Digestive&Kidney DiseasesNational Institutes of HealthBethesdaMDUSA

Hepatitis D is the most severe form of viral hepatitis associated with a more rapid progression to cirrhosis and an increased risk of hepatocellular carcinoma and mortality compared with hepatitis B *** once thought of as a disappearing disease,hepatitis D is now becoming recognized as a serious worldwide issue due to improvement in diagnostic testing and immigration from endemic *** these concerns,there is currently only one accepted medical therapy(pegylated-interferon-a)for the treatment of hepatitis D with less than desirable efficacy and significant side *** to these reasons,many patients never undergo ***,increasing knowledge about the virus and its life cycle has led to the clinical development of multiple promising new therapies that hope to alter the natural history of this disease and improve patient *** this article,we will review the literature from discovery to the current investigational therapies.

关键词： Hepatitis D hepatitis B epidemiology natural history outcome treatment novel therapies

Expression of Kaposi's Sarcoma-associated Herpesvirus ORFK8.1 and Its Preliminary Diagnostic Application

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Virologica Sinica 2009年第3期24卷 202-208页

作者： Bi-shi FU Bao-lin LI Xin-xing OUYANG Yan ZENG Fan-hong XU Lin-ding WANG State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences Wuhan 430071China Graduate School of the Chinese Academy of Sciences Beejing 100039 China Shanghai institute of Biological Products Shanghai 200052 China Laboratory of Xinjiang endemic and Ethnic diseases Shihezi University Shihezi 832002 China

The ORFK8.1 of Kaposi＇s sarcoma associated-herpesvirus （KSHV） was expressed in a prokaryotic expression system. The expression of recombinant *** containing pQE-80L-orf KS.1 was induced by isopropyl-b-D-thiogalactopyranoside （IPTG）. The fusion protein was purified by chromatyography. The expressed protein and its purified product were identified by sodium dodecyl sulfate-polyacrylamide gel eletrophoresis （SDS-PAGE）. SDS-PAGE showed that a protein of 26 kDa was visualized as expected. A western blot assay was established to analyze the immunogenicity of purified recombinant ORFKS. 1 protein. The optimal condition of the recombinant ORFKS. 1 ELISA assay was confirmed： the concentration of antigen was 5 μg/mL, the dilution of serum was 1：200. We used the ELISA method to investigate the recombinant ORF KS. 1 protein＇s specificity, the data showed that the specificity of ORF KS.1 to detect KSHV was 100%. At the same time, 560 sera samples from Hubei province were detected by using ORFKS. 1 ELISA to investigate KSHV seroprevalence in this region. The KSHV seroprevalence in Hubei province is shown to be 6.80%.

关键词： Kaposi's sarcoma-associated herpesvirus (KSHV) ORFKS. 1 Enzyme-linked immunosorbent assays (ELISA) Seroprevalence

Different Strategies for Preparation of Non-tagged rV270 Protein and Its Efficacy against Yersinia Pestis Challenge

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Biomedical and Environmental Sciences 2010年第5期23卷 333-340页

作者： WANG WANG ZHI-ZHEN QI QING-WEN ZHANG BEN-CHUAN WU ZI-WEN ZHU YONG-HAI YANG BAI-ZHONG CUI RUI-XIA DAI YE-FENG QIU ZU-YUN WANG ZHAO-BIAO GUO TAO-XING SHI HU WANG RUI-FU YANG XIAO-YI WANG Laboratory of Analytical Microbiology State Key Laboratory of Pathogen and BiosecurityBeijing Institute of icrobiology and EpidemiologyBeijing 100071China Qinghai institute for endemic Disease Prevention and Control of Qinghai Province Xining811602China Beijing institute of Bioengneering Beijing 100071China

Objective LcrV is an important component for the development of a subunit vaccine against *** reduce immunosuppressive activity of LcrV,a recombinant LcrV variant lacking amino acids 271 to 326 （rV270） was prepared by different methods in this *** A new strategy that produced non-tagged or authentic rV270 protein was designed by insertion of rV270-thrombin-hexahistidine fusion gene into the vector pET24a,or by insertion of hexahistidine-enterokinase-rV270 or hexahistitine-factor Xa-rV270 fusion gene into the vector *** Co2＋ affinity chromatography,a purification strategy was developed by cleavage of His tag on column,following Sephacryl S-200HR column filtration *** Removal of His tag by thrombin,enterokinase and factor Xa displayed a yield of 99.5%,32.4% and 15.3%,*** Sephacryl S-200HR column filtration chromatography,above 97% purity of rV270 protein was *** rV270 that was adsorbed to 25% （v/v） Al（OH）3 adjuvant in phosphate-buffered saline （PBS） induced very high titers of antibody to rV270 in BALB/c mice and protected them （100% survival） against subcutaneous challenge with 106 CFU of *** virulent strain *** The completely authentic rV270 protein can be prepared by using enterokinase or factor Xa,but they exhibited extremely low cleavage activity to the corresponding recognition *** cleavage is an efficient strategy to prepare non-tagged rV270 protein and can be easily operated in a large scale due to its relatively low cost and high cleavage *** recombinant rV270 can be used as a key component to develop a subunit vaccine of plague.

关键词： Yersinia pestis rV270 antigen Purification Protection Plague

Molecular phylogeny of trematodes in Family Heterophyidae based on mitochondrial cytochrome c oxidase subunit Ⅰ(mCO Ⅰ)

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Asian Pacific Journal of Tropical Medicine 2014年第6期7卷 446-450页

作者： Thapana Chontananarth Chalobol Wongsawad Siriwadee Chomdej Duangduen Krailas Jong Yil Chai Applied Parasitology Research Laboratory Department of BiologyFaculty of Science.Chiang Mai University Department of Biology Faculty of Science Silpakorn University Department of Parasitology and Tropical Medicine Seoul National University College of Medicine institute of endemic diseases Seoul National University Medical Research Center

Objective:To analyze a phylogenetic tree for understanding the molecular systematic of trematode in Pamily *** are highly distributed in ***:Based on thirteen sequences of mitochondrial cytochrome C oxidase subunit 1(mCO I) gene from six genera of heterophyid trernatodes,***,Stellantchasmus,Centrocestus,Metagonimus,Pygidopsis,and Haplnrchoides were aligned automatically using the Clustal × 2.0 program.A phylogenetic tree was constructed by maximum likeiughood(ML) and neighbor-joining(NJ)methods,with 1000 bootstrap using the 5.0 ***:The phylogenetic relationship from both methods was similar and separated into three groups consisting of Haplorchoides pumilio group,Haplorchoides taichui group and another heterophyid ***:The sequence data of mtCOl can he used to investigate the phylogenetic relationships of trernatodes at the genus *** clade of different genera of heterophyid trernatodes can be separated into sister groups that correlated with the morphological characteristic,kind of secondary intermediate host and geographic distribution.

关键词： Phylogenetic relationship mCOI Heterophyidae

Genetic diversity and prevalence of emerging Rickettsiales in Yunnan Province:a large-scale study

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Infectious diseases of Poverty 2024年第4期13卷 97-98页

作者： Chun-Hong Du Rong Xiang Shuang-Shuang Bie Xing Yang Ji-Hu Yang Ming-Guo Yao Yun Zhang Zhi-Hai He Zong-Ti Shao Chun-Feng Luo En-Nian Pu Yu-Qiong Li Fan Wang Zhi Luo Chao-Bo Du Jie Zhao Miao Li Wu-Chun Cao Yi Sun Jia-Fu Jiang Yunnan Key Laboratory for Zoonosis Control and Prevention Yunnan Institute for Endemic Diseases Control and Prevention Dali PR China State Key Laboratory of Pathogen and Biosecurity Academy of Military Medical Sciences Beijing PR China Department of Medical Microbiology and Immunology School of Basic Medicine Dali University Dali PR China

Background Rickettsia and related diseases have been identified as significant global public health *** study involved comprehensive field and systematic investigations of various rickettsial organisms in Yunnan *** Between May 18,2011 and November 23,2020,field investigations were conducted across 42 counties in Yunnan Province,China,encompassing small mammals,livestock,and *** screenings for Rickettsiales involved amplifying the 16S rRNA genes,along with additional genus-or species-specific genes,which were subse-quently confrmed through sequencing *** comparisons were carried out using the Basic Local Align-ment Search Tool(BLAST).Phylogenetic relationships were analyzed using the default parameters in the Molecular Evolutionary Genetics Analysis(MEGA)*** chi-squared test was used to assess the diversities and compo-nent ratios of rickettsial agents across various *** A total of 7964 samples were collected from small mammals,livestock,and ticks through Yunnan Province and submitted for screening for rickettsial *** rickettsial species from the genera Rickettsia,Anaplasma,Ehrlichia,Neoehrlichia,and Wolbachia were detected,with an overall prevalence of 14.72%.Among these,11 species were identifed as pathogens or potential pathogens to humans and ***,10 rickettsial organisms were widely found in 42.11%(24 out of 57)of small mammal *** prevalence was observed in Dremomys samples at 5.60%,in samples from regions with latitudes above 4000 m or alpine meadows,and in those obtained from Yuanmou *** phagocytophilum and Candidatus Neoehrlichia mikurensis were broadly infect-ing multiple genera of animal *** contrast,the small mammal genera Neodon,Dremomys,Ochotona,Anouro-sorex,and Mus were carrying individually specific rickettsial agents,indicating host *** were 13 rickettsial species detected in 57.14%(8 out of 14)of tick species,w

关键词： Rickettsiales Candidatus Rickettsia longicorni Tick Small mammal China