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Characterization of a β-1,3-glucan recognition protein from the beet armyworm, Spodoptera exigua （Insecta： Lepidoptera： Noctuidae）

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Insect Science 2013年第5期20卷 575-584页

作者： Kyeongrin Bang Sujin Park Saeyoull Cho Department of Applied biology College of Agriculture and Life Science Agriculture and Life Sciences Research Institute Kangwon National University Chuncheon CHA Cancer institute CHA University 605 Yeoksam-dong Gangnam-gu Seoul 135-081 Korea

The β-1,3-glucan recognition protein gene from Spodoptera exigua （SeβGRP） was cloned and characterized. The cDNA of this gene is 1？644 nucleotides in length and the predicted polypeptide is 491 amino acids （aa） in length, with a calculated molecular mass of 54.8 kDa. The first 22 aa encode a predicted secretion signal peptide. A BLAST search, multiple sequence alignment, and phylogenetic analysis of the aa sequence of SeβGRP revealed that this protein is most similar to the β-1,3-glucan recognition protein （βGRP） family of pattern recognition proteins. Using reverse-transcription polymerase chain reaction, we detected the presence of SeβGRP transcripts in the egg, larval, pupal, and adult stages of S. exigua. In addition, the SeβGRP transcript was expressed in all the tissues examined including the brain, hemocytes, fat body, intestine, and cuticle. There were no changes in SeβGRP mRNA levels in larvae infected with ultraviolet （UV）-killed Escherichia coli DH5α compared with the control larvae inoculated with the water; however, SeβGRP mRNA levels were markedly elevated 4–8 h after infection and slightly induced 12–24 h after infection in larvae injected with UV-killed Fusarium oxysporum. This may be because β-1,3-glucan is the main component of the cell wall of F. oxysporum, but not E. coli DH5α.

关键词： β-1,3-glucan recognition protein Fusarium oxysporum immune,Spodoptera exigua

A Copper(Ⅱ)Complex Based on N-(4-hydroxybenzyl)-L-serine:Synthesis,Crystal Structure and Inhibitory Activity on PTP1B and TCPTP

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Chinese Journal of Structural Chemistry 2016年第11期35卷 1686-1693页

作者：李艳红卢丽萍朱苗力袁彩霞冯思思高增强 institute of Molecular Science Key Laboratory of Chemical Biology and Molecular Engineering of the Education MinistryShanxi University Fenyang College Shanxi Medical University Fenyang Beijing Synchrotron Radiation Facility Institute of High Energy PhysicsChinese Academy of Sciences

A novel copper（II） complex with the reduced Schiff base, [Cu（L）2]·H2O （I, HL = N-（4-hydroxybenzyl）-L-serine）, was prepared in aqueous solution and characterized by elemental analysis, FT-IR, electrospray ionization mass spectrometry and single-crystal X-ray diffraction. Complex I crystallizes in the orthorhombic system, space group P212121, with a = 8.9040（18）, b = 9.1530（18）, c = 24.891（5）A^°, V = 2028.6（7） A^°3, Z = 4, C20H26CuN2O9, Mr = 501.97, Dc = 1.644g·cm^3, μ = 1.135 mm^-1, F（000） = 1044, GOOF = 1.194, the final R = 0.0484 and wR = 0.1420 for 6186 observed reflections （I 〉 2σ（I））. In I, two L^- anions are coordinated to the copper ion in tridentate and bidentate chelating modes, respectively, resulting in the coordinated geometry of copper ion to be a distorted square pyramid. The intermolecular hydrogen bonds between the complexes, complexes and lattice water molecules lead to a 2D supramolecular network. The bioactivity of the complex as a potential PTPs inhibitory agent in vitro was investigated, displaying potent inhibition against PTP1B （IC50, 0.27 μM） and TCPTP （IC50, 0.57 μM） with a moderate selectivity.

关键词： copper(Ⅱ) complex N-(4-hydroxybenzyl)-L-serine crystal structure inhibitor,PTP1B TCPTP

Increasing the heterologous production of spinosad in Streptomyces albus J1074 by regulating biosynthesis of its polyketide skeleton

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Synthetic and Systems Biotechnology 2021年第4期6卷 292-301页

作者： Ziheng An Hui Tao Yong Wang Bingqing Xia Yang Zou Shuai Fu Fang Fang Xiao Sun Renqiong Huang Yao Xia Zixin Deng Ran Liu Tiangang Liu Key Laboratory of Combinatorial Biosynthesis and Drug Discovery Ministry of Education and Wuhan University School of Pharmaceutical SciencesWuhan430071PR China CAS Key Laboratory of Quantitative Engineering biology Shenzhen Institute of Synthetic BiologyShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen518055PR China Hubei Engineering Laboratory for Synthetic Microbiology Wuhan Institute of BiotechnologyWuhan430075PR China

Spinosyns are natural broad-spectrum biological insecticides with a double glycosylated polyketide structure that are produced by aerobic fermentation of the actinomycete,Saccharopolyspora ***,their large-scale overproduction is hindered by poorly understood bottlenecks in optimizing the original strain,and poor adaptability of the heterologous strain to the production of *** this study,we genetically engineered heterologous spinosyn-producer Streptomyces albus J1074 and optimized the fermentation to improve the production of spinosad(spinosyn A and spinosyn D)based on our previous *** systematically investigated the result of overexpressing polyketide synthase genes(spnA,B,C,D,E)using a constitutive promoter on the spinosad titer in *** *** supply of polyketide synthase precursors was then increased to further improve spinosad ***,increasing or replacing the carbon source of the culture medium resulted in a final spinosad titer of~70 mg/L,which is the highest titer of spinosad achieved in heterologous Streptomyces *** research provides useful strategies for efficient heterologous production of natural products.

关键词： Spinosyn Spinosad Polyketide Polyketide synthase Heterologous production Streptomyces

Genome-wide identification of OSCA gene family and their potential function in the regulation of dehydration and salt stress in Gossypium hirsutum

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Journal of Cotton Research 2019年第2期2卷 87-104页

作者： YANG Xiu XU Yanchao YANG Fangfang MAGWANGA Richard Odongo CAI Xiaoyan WANG Xingxing WANG Yuhong HOU Yuqing WANG Kunbo LIU Fang ZHOU Zhongli institute of Cotton Research Chinese Academy of Agricultural Science (ICRCAAS)/Research Base in Anyang Institute of TechnologyState Key Laboratory of Cotton Biology

Background:Cotton(Gossypium hirsutum) provides the largest natural fiber for the textile manufacturing industries,but its production is on the decline due to the effects of *** salt-alkalization leads to damage in cotton growth and a decrease in ***-gated calcium-permeable channels(OSCA) have been found to be involved in the detection of extracellular changes which trigger an increase in cytosolic free calcium ***-induced calcium ion increases have been widely speculated to be playing a role in osmosensing in ***,the molecular nature of the corresponding calcium ion channels remains *** this research work,we describe the OSCA genes and their putative function in osmosensing in plants by carrying out genomewide identification,characterization and functional analysis of the significantly up-regulated OSCA gene,GhOSCA1.1 through reverse ***:A total of 35,21 and 22 OSCA genes were identified in ***,***,and *** genomes,respectively,and were classified into four different clades according to their gene structure and phylogenetic *** and protein structure analysis indicated that 35 GhOSCA genes contained a conserved RSN17 TM(PF02714) ***,the cis-regulatory element analysis indicated that the OSCA genes were involved in response to abiotic ***,the knockdown of one of the highly up-regulated genes,GhOSCA1.1 showed that the virus-induced gene silenced(VIGS) plants were highly sensitive to dehydration and salinity stresses compared with the none VIGS plants as evident with higher concentration levels of oxidant enzymes compared with the antioxidant enzymes on the leaves of the stressed ***:This study provides the first systematic analysis of the OSCA gene family and will be important for understanding the putative functions of the proteins encoded by the OSCA genes in *** results provide a new insight of de

关键词： OSCA gene family Gossypium hirsurum VIGS Salt and dehydration stress

Extracellular vesicles in the diagnosis and treatment of central nervous system diseases

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Neural Regeneration Research 2020年第4期15卷 586-596页

作者： Alisa A.Shaimardanova Valeriya V.Solovyeva Daria S.Chulpanova Victoria James Kristina V.Kitaeva Albert A.Rizvanov institute of Fundamental Medicine and biology Kazan Federal UniversityKazanRussia Shemyakin-Ovchinnikov institute of Bioorganic Chemistry The Russian Academy of SciencesMoscowRussia School of Veterinary Medicine and Science University of NottinghamSutton Bonington CampusLeicestershireUK

Extracellular vesicles,including exosomes and microvesicles,play a fundamental role in the activity of the nervous system,participating in signal transmission between neurons and providing the interaction of central nervous system with all body *** many neurodegenerative diseases,neurons pack toxic substances into vesicles and release them into the extracellular space,which leads to the spread of misfolded neurotoxic *** contents of neuron-derived extracellular vesicles may indicate pathological changes in the central nervous system,and the analysis of extracellular vesicle molecular content contributes to the development of non-invasive methods for the diagnosis of many central nervous system *** vesicles of neuronal origin can be isolated from various biological fluids due to their ability to cross the blood-brain ***,the diagnostic potential of almost all toxic proteins involved in nervous system disease pathogenesis,specificallyα-synuclein,tau protein,superoxide dismutase 1,FUS,leucine-rich repeat kinase 2,as well as some synaptic proteins,has been well *** attention is paid to extracellular RNAs mostly associated with extracellular vesicles,which are important in the onset and development of many neurodegenerative *** on parental cell type,extracellular vesicles may have different therapeutic properties,including neuroprotective,regenerative,and *** to nano size,biosafety,ability to cross the blood-brain barrier,possibility of targeted delivery and the lack of an immune response,extracellular vesicles are a promising vehicle for the delivery of therapeutic substances for the treatment of neurodegenerative diseases and drug delivery to the *** review describes modern approaches of diagnosis and treatment of central nervous system diseases using extracellular vesicles.

关键词： biomarkers cell-mediated therapy central nervous system diseases diagnosis exosomes extracellular RNAs extracellular vesicles microRNAs microvesicles neurodegenerative diseases

CO_2 Flux Estimation by Different Regression Methods from an Alpine Meadow on the Qinghai-Tibetan Plateau

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Advances in Atmospheric Sciences 2010年第6期27卷 1372-1379页

作者：姜春明于贵瑞曹广民李英年张世春方华军 Key Laboratory of Ecosystem Network Observation and Modeling Chinese Academy of SciencesInstitute of Geographical Sciences and Natural Resources ResearchChinese Academy of Sciences Graduate University of the Chinese Academy of Sciences Northwest Plateau institute of biology Chinese Academy of Sciences

CO2 efflux was estimated using different regression methods in static chamber observation from an alpine meadow on the Qinghai-Tibetan Plateau. The CO2 efflux showed a seasonal pattern, with the maximun flux occurring in the middle of July. The temperature sensitivity of CO2 efflux （Q10） was 3.9, which was at the high end of the range of global values. CO2 emissions calculated by linear and nonlinear regression were significantly different （p 〈0.05）. Compared with the linear regression, CO2 emissions calculated by exponential regression and quadratic regression were 12.7% and 11.2% larger, respectively. However, there were no significant differences in temperature sensitivity values estimated by the three methods. In the entire growing season, the CO2 efflux estimated by linear regression may be underestimated by up to 25% compared to the real CO2 efflux. Consequently, great caution should be taken when using published flux data obtained by linear regression of static chamber observations to estimate the regional CO2 flux in alpine meadows on the Qinghai-Tibetan Plateau.

关键词： CO2 emission static chamber technique nonlinearity underestimation Qinghai-Tibetan Plateau

Improving pathway prediction accuracy of constraints-based metabolic network models by treating enzymes as microcompartments

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Synthetic and Systems Biotechnology 2023年第4期8卷 597-605页

作者： Xue Yang Zhitao Mao Jianfeng Huang Ruoyu Wang Huaming Dong Yanfei Zhang Hongwu Ma Tianjin institute of Industrial Biotechnology Chinese Academy of SciencesTianjin300308China National Technology Innovation Center of Synthetic biology Tianjin300308China School of Environmental Ecology and Biological Engineering Wuhan Institute of TechnologyWuhan430205China

Metabolic network models have become increasingly precise and accurate as the most widespread and practical digital representations of living *** prediction functions were significantly expanded by integrating cellular resources and abiotic constraints in recent ***,if unreasonable modeling methods were adopted due to a lack of consideration of biological knowledge,the conflicts between stoichiometric and other constraints,such as thermodynamic feasibility and enzyme resource availability,would lead to distorted *** this work,we investigated a prediction anomaly of EcoETM,a constraints-based metabolic network model,and introduced the idea of enzyme compartmentalization into the analysis *** rational combination of reactions,we avoid the false prediction of pathway feasibility caused by the unrealistic assumption of free intermediate *** allowed us to correct the pathway structures of L-serine and L-tryptophan.A specific analysis explains the application method of the EcoETM-like model and demonstrates its potential and value in correcting the prediction results in pathway structure by resolving the conflict between different constraints and incorporating the evolved roles of enzymes as reaction ***,this work also reveals the trade-off between product yield and thermodynamic *** work is of great value for the structural improvement of constraints-based models.

关键词： Genome-scale metabolic network models (GEMs) Enzymatic and thermodynamic constraints Thermodynamic driving force(MDF) Compartmentalization Multifunctional enzymes Enzyme complexes

Promoter hypermethylation and loss of CD133 gene expression in colorectal cancers

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World Journal of Gastroenterology 2010年第25期16卷 3153-3160页

作者： You-Kyung Jeon Sung-Hee Kim Seung-Ho Choi Kyung-Hee Kim Byong-Chul Yoo Ja-Lok Ku Jae-Gahb Park Laboratory of Cell biology Cancer Research Institute Seoul National University College of Medicine Seoul 110-799 South Korea Colorectal Cancer Branch Research Institute National Cancer Center Goyang 411-764 Gyeonggi South Korea

AIM: To understand CD133 promoter hypermethyl-ation and expression in 32 colorectal cancer cell lines. METHODS: Nucleic acid was isolated from 32 colorectal cancer cell lines and CD133 expression levels were measured by reverse transcription-polymerase chain reaction (RT-PCR) and real-time PCR. Promoter methylation status of the CD133 gene was analyzed with a methylation-specific PCR after sodium-bisulfi te modification and by clonal sequencing analysis. The correlation between expression and promoter methylation of CD133 gene was confirmed with treatment of 5-aza-2’-deoxycytidine. RESULTS: We measured CD133 expression levels in 32 colorectal cancer cell lines. RT-PCR analysis showed undetectable or low levels of CD133 expression in 34.4%of cell lines. To verify the relation between CD133 expression and methylation status of the CD133 gene promoter in colorectal carcinogenesis, CD133 gene promoter hypermethylation was analyzed in 32 cancer cell lines. Promoter hypermethylation was detected in 13 (40.6%) of the cell lines using methylation specificPCR and confirmed by bisulfite sequencing analysis. Treatment of 11 of the cell lines with the demethylation agent 5-aza-2’-deoxycytidine recovered CD133 expression in most of them. CONCLUSION: Transcriptional repression of CD133 is caused by promoter hypermethylation of the CD133 CpG islands in some of colorectal cancer cell lines. The study may contribute to the understanding of the role of CD133 inactivation in the progression of colorectal cancers.

关键词： CD133 Promoter Hypermethylation Colorectal cancer Sodium bisulfite modification

Ranging behavior of the François’langur(Trachypithecus francoisi)in limestone habitats of Nonggang,China

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Integrative Zoology 2011年第2期6卷 157-164页

作者： Qihai ZHOU Chengming HUANG Ming LI Fuwen WEI Key Laboratory of Animal Ecology and Conservation biology Institute of ZoologyChinese Academy of SciencesBeijingChina College of Sciences Guangxi Normal UniversityGuilinChina National Zoological Museum Institute of ZoologyChinese Academy of SciencesBeijingChina

Ranging behavior is an important aspect of animal behavior that researchers use to investigate ecological influences on individual *** collected data on the ranging behavior of one group of François’langurs(Trachypithecus francoisi)between August 2003 and July 2004 in a limestone habitat within Nonggang Nature Reserve,*** evaluated the influences of food availability,water resources and sleeping sites on ranging *** the study period,the total home range size for the study group was 64.5 or(69.3 ha if the lacunae within the borders in which langurs were not observed were included),and the mean daily path length was 541 *** are well within the range of variation reported in Trachypithecus *** monthly range size was between 9.8 and 23.3 ha,and monthly range size correlated negatively with young leaf *** langurs tended to use a larger range size during young leaf-lean *** langurs did not use their home range uniformly,and 74%of their activities occurred within 35%of their home *** most heavily used quadrats in the home range were located near the most frequently used sleeping sites,suggesting that sleeping sites have a significant influence on the ranging behavior of François’langurs in limestone habitats.

关键词： François’langur limestone habitat ranging behavior Trachypithecus francoisi