检索结果-南通市图书馆

Controlling self-assembling and tumor cell-targeting of protein-only nanoparticles through modular protein engineering

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China Materials 2020年第1期63卷 147-156页

作者： Eric Volta-Duran Olivia Cano-Garrido Naroa Serna Hector Lopez-laguna laura Sanchez-Garcia Mireia Pesarrodona Alejandro Sanchez-Chardi Ramon Mangues Antonio Villaverde Esther Vazquez ugutz unzueta institut de Biotecnologia i de Biomedicina Universitat Autònoma de BarcelonaBellaterra08193 BarcelonaSpain departament de Genetica i de Microbiologia Universitat Autònoma de BarcelonaBellaterra08193 BarcelonaSpain CIBER de Bioingeniería Biomateriales y Nanomedicina(CIBER-BBN)C/Monforte de Lemos 3-528029 MadridSpain Nanoligent SL Edifici EUREKAUniversitat Autonoma de BarcelonaBellaterra08193 BarcelonaSpain institut dInvestigacions Biomèdiques Sant Pau and Josep Carreras Research institute Hospital de la Santa Creu i Sant Pau08041 BarcelonaSpain departament de Biologia Evolutiva Ecologia i Ciencies AmbientalsFacultat de BiologiaUniversitat de BarcelonaAv.Diagonal 64308028 BarcelonaSpain institute for Research in Biomedicine(IRB Barcelona) The Barcelona Institute of Science and Technology08028 BarcelonaSpain

Modular protein engineering is suited to recruit complex and multiple functionalities in single-chain polypeptides. Although still unexplored in a systematic way, it is anticipated that the positioning of functional domains would impact and refine these activities, including the ability to organize as supramolecular entities and to generate multifunctional protein materials. To explore this concept, we have repositioned functional segments in the modular protein T22-GFP-H6 and characterized the resulting alternative fusions. In T22-GFP-H6, the combination of T22 and H6 promotes selfassembling as regular nanoparticles and selective binding and internalization of this material in CXCR4-overexpressing tumor cells, making them appealing as vehicles for selective drug delivery. The results show that the pleiotropic activities are dramatically affected in module-swapped constructs, proving the need of a carboxy terminal positioning of H6 for protein self-assembling, and the accommodation of T22 at the amino terminus as a requisite for CXCR4^+ cell binding and internalization. Furthermore, the failure of self-assembling as regular oligomers reduces cellular penetrability of the fusions while keeping the specificity of the T22-CXCR4 *** these data instruct how multifunctional nanoscale protein carriers can be designed for smart, protein-driven drug delivery, not only for the treatment of CXCR4^+ human neoplasias, but also for the development of anti-HIV drugs and other pathologies in which CXCR4 is a relevant homing marker.

关键词： nanoparticles protein materials recombinant proteins drug delivery self-assembling cancer cell targeting

Expert group syndrome at high altitude

在线全文

学校读者我要写书评

暂无评论

Sports Medicine and Health Science 2022年第3期4卷 215-218页

作者： Jean-Paul Richalet Marie-Anne Magnan Alice Gavet Pierre lafère universitéSorbonne Paris Nord Laboratoire Hypoxie et PoumonUMR INSERM U1272BobignyFrance institut national des Sports de l’Expertise et de la PerformancePôle médicalParisFrance Hôpitaux universitaires de Genève Département de médecine aiguëUnitéde médecine subaquatique et hyperbareGenevaSwitzerland Hôpitaux du Mont-Blanc Soins de suite et réadaptationChamonixFrance universitéde Bretagne Occidentale Laboratoire ORPHYEA 44324BrestFrance HauteÉcole Bruxelles-Brabant(HE2B) EnvironmentalOccupationalAgeing(Integrative)Physiology LaboratoryBrusselsBelgium

During a training session for the university diploma of Mountain medicine delivered by university Sorbonne Paris Nord for medical doctors,one of the participants developed signs of maladaptation to high altitude at 3600 m,the severity of which was incorrectly *** was sparingly given by the patient(an anesthetist)to several of his colleagues and no one was in charge to collect clinical data,take a history,and provide appropriate *** combination of the absence of designation of a supervising doctor and the difficulty of communicating with the patient led to a lack of coordinated management and to an evolution of the symptoms towards severe acute mountain ***,the very rapid management of the patient and a rapid helicopter evacuation,as soon as the symptoms worsened towards the onset of a suspected high altitude cerebral and/or pulmonary edema,allowed rapid resolution without ***,medical,psychological,and managerial factors led to this Expert Group Syndrome.

关键词： Acute mountain sickness High altitude cerebral edema Psychology Group management

Kernel principal component analysis network for image classification

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Southeast university(English Edition) 2015年第4期31卷 469-473页

作者：吴丹伍家松曾瑞姜龙玉 Lotfi Senhadji 舒华忠东南大学计算机网络和信息集成教育部重点实验室南京210096 中法生物医学信息研究中心南京210096 institut national de la Santé et de la recherche Médicale u 1099 laboratoire Traitement du Signal et de l'Image universitéde Rennes 1 laboratoire Traitement du Signal et de l'Image université de Rennes 1

In order to classify nonlinear features with a linear classifier and improve the classification accuracy, a deep learning network named kernel principal component analysis network（ KPCANet） is proposed. First, the data is mapped into a higher-dimensional space with kernel principal component analysis to make the data linearly separable. Then a two-layer KPCANet is built to obtain the principal components of the image. Finally, the principal components are classified with a linear classifier. Experimental results showthat the proposed KPCANet is effective in face recognition, object recognition and handwritten digit recognition. It also outperforms principal component analysis network（ PCANet） generally. Besides, KPCANet is invariant to illumination and stable to occlusion and slight deformation.

关键词： deep learning kernel principal component analysis net(KPCANet) principal component analysis net(PCANet) face recognition object recognition handwritten digit recognition

Site-directed cysteine coupling of disulfide-containing non-antibody carrier proteins(THIOCAPs)

在线全文

学校读者我要写书评

暂无评论

Science China Materials 2023年第10期66卷 4109-4120页

作者： Ariana Rueda Julian I.Mendoza Lorena Alba-Castellon Eloi Parladé Eric Voltà-Durán David Paez Anna Avino Ramon Eritja Esther Vázquez Antonio Villaverde Ramón Mangues ugutz unzueta institut d'Investigació Biomèdica Sant Pau(IIB Sant Pau) Sant Quintí 77-7908041 BarcelonaSpain Josep Carreras Leukaemia Research institute(IJC Campus Sant Pau) 08041 BarcelonaSpain CIBER de Bioingeniería Biomateriales y NanomedicinaInstituto de Salud Carlos Ⅲ28029 MadridSpain institut de Biotecnologia i de Biomedicina Universitat Autònoma de Barcelona08193 BellaterraSpain departament de Genètica i de Microbiologia Universitat Autònoma de Barcelona08193 BellaterraSpain department of Medical Oncology Hospital de la Santa Creu i Sant Pau.Barcelona08025 BarcelonaSpain CIBER de Enfermedades Raras(CIBERER) Instituto de Salud Carlos Ⅲ28029 MadridSpain institute for Advanced Chemistry of Catalonia(IQAC) CSIC08034 BarcelonaSpain

The development of a new generation of nonantibody protein drug delivery systems requires site-directed conjugation strategies to produce homogeneous,reproducible and scalable *** that,the genetic addition of cysteine residues into solvent-exposed positions allows the thiol-mediated cysteine coupling of therapeutic drugs into protein-based ***,the high reactivity of unpaired cysteine residues usually reduces protein stability,consequently imposing the use of more methodologically demanding purification *** is especially relevant for disulfide-containing nanocarriers,as previously observed in ***,although many protein scaffolds and targeting ligands are also rich in disulfide bridges,the use of these methodologies over emerging non-antibody carrier proteins has been completely ***,we report the development of a simple and straightforward procedure for a onestep production and site-directed cysteine conjugation of disulfide-containing non-antibody thiolated carrier proteins(THIOCAPs).This method is validated in a fluorescent C-X-C chemokine receptor 4(CXCR4)-targeted multivalent nanocarrier containing two intramolecular disulfide bridges and one reactive cysteine residue strategically placed into a solvent-exposed position(THIO-T22-GFP-H6)for drug conjugation and in a humanized alternative intended for clinical applications(T22-HSNBT-H6).Thus,we produce very stable,homogeneous and fully functional antitumoral nanoconjugates(THIO-T22-GFP-H6-MMAE and T22-HSNBT-H6-MMAE)that selectively eliminate target cancer cells via ***,the developed methodology appears as a powerful tool for the rational engineering of emerging non-antibody,cell-targeted protein nanocarriers that contain disulfide bridges together with a solvent-exposed reactive cysteine(THIOCAP).This should pave the way for the development of a new generation of stable,homogeneous and efficient nanomedicines.

关键词： disulfide directed bridges

Activation of dorsal raphe neurons produces strong reward and guides rapid learning

在线全文

学校读者我要写书评

暂无评论

Activation of dorsal raphe neurons produces strong reward an...

Stem cell transplantation in cerebrovascular accidents:A global bibliometric analysis(2000-2023)

中国神经科学学会第十届全国学术会议

作者： Zhi-Xiang LIu Jing-Feng ZHOu Yi LI Fei Hu Yao Lu Ming MA Qi-Ru FENG Ju-En ZHANG Jia-Wei ZENG Jun-Hong BAO Ji-Young KIM Zhou-Feng CHEN Salah El MESTIKAWY Minmin LuO national institute of Biological Sciences College of Life Sciences Beijing Normal University PTN Graduate Program School of Life SciencesPeking University Graduate School of Peking union Medical College Center for the Study of Itch and Departments of AnesthesiologyPsychiatryand Developmental BiologyWashington University School of Medicine institut national de la sante et de la recherche medicale(INSERM) School of Life Sciences Tsinghua University

Reward processing is a fundamental role of the *** midbrain dorsal raphe nucleus（DRN） composed of the main resource of central serotonin（5-hydroxytryptamine;5-HT） and provides extensive innervation to the *** it has been hypothesized that 5-HT neurons oppose dopamine system to inhibit reward and encode pumshment,the exact role of DRN in reward signaling is remaining *** we show that activation of DRN neurons produces strong reward and effectively guides learning by releasing glutamate and 5-HT to activate downstream target VTA and *** optogenetics,slice recording with multiple reward related behavior tests,we found that neuron-specific activation of DRN neurons could reinforce animal to explore the designated spatial area,efficiently direct odor discrimination learning,shift natural sucrose preference and furthermore intensively self-stimulate DRN in an oprant *** DRN neurons are traditionally thought to release 5-HT,DRN stimulation released glutamate *** analysis of two mutant mouse lines indicates that both of the neurotransmitters contributed to reward signahng and terminal stimulation in reward-related downstream targets elicits obvious conditioned place *** results suggest that DRN may represent a new reward center besides the traditional dopamine system and provide a different perspective of understanding the normal and pathological roles of the DRN in stress control and human depression.

关键词： reward DRN optogenetics serotonin and glutamate behavior

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

World Journal of Stem Cells 2024年第9期16卷 832-841页

作者： Jad El Masri Ahmad Afyouni Maya Ghazi Karim Hamideh Israe Moubayed Abdo Jurjus Hanine Haidar Ruzanna Petrosyan Pascale Salameh Hassan Hosseini École Doctorale Sciences de la Vie et de la Santé UniversitéParis-Est CréteilCréteil 94010France INSERM u955-E01 Institut Mondor de Recherche BiomédicaleUniversitéParis-Est CréteilCréteil 94000France Faculty of Medical Sciences Lebanese UniversityBeirut 1533Lebanon department of Anatomy Cell Biology and Physiological SciencesFaculty of MedicineAmerican University of BeirutBeirut 1107Lebanon department of Neurology Faculty of MedicineLebanese American UniversityBeirut 1102Lebanon department of Pathology Faculty of MedicineLebanese American UniversityBeirut 1102Lebanon Faculty of Pharmacy Lebanese UniversityBeirut 1102Lebanon Faculty of Medicine Lebanese American UniversityBeirut 1102Lebanon department of Primary Care and Population Health University of Nicosia Medical SchoolNicosia 2408Cyprus institut national de SantéPublique d'Épidémiologie Clinique et de Toxicologie-Liban Beirut 1103Lebanon department of Neurology Henri Mondor HospitalAP-HPCréteil 94000France

BACKGROuND Cerebrovascular accident(CVA)is a major global contributor to death and *** part of its medical management,researchers have recognized the importance of promising neuroprotective strategies,where stem cell transplantation(SCT)is thought to confer advantages via trophic and neuroprotective *** To evaluate the current state of research on SCT in patients with CVA,assess key trends and highlight literature *** PubMed was screened for SCT in CVA-related articles in October 2023,for each country during the period between 2000 and *** the World Bank data,total population and gross domestic product were collected for ***_1.6.19 was used to create the VOS figure using the results of the same *** and tables were obtained using Microsoft Office *** A total of 6923 studies were identified on SCT in CVA,making 0.03%of all published studies ***,68%were conducted in high-income countries,with a significant focus on mesenchymal stem *** journal“Stroke”featured the largest share of these articles,with mesenchymal SCT having the highest rate of inclusion,followed by hematopoietic *** time,there has been a noticeable shift from in vitro studies,which assess stem cell proliferation and neurogenesis,to in vivo studies aimed at evaluating efficacy and ***,the number of reviews increased along this *** This bibliometric analysis provides a comprehensive guide for physicians and researchers in the field through an objective overview of research activity,and highlights both current trends and *** a potential therapeutic role in CVA,more research is needed in the future to focus on different aspects of SCT,aiming to reach a better treatment strategy and improve life quality in patients.

关键词： Bibliometric analysis PubMed Stem cell transplantation Cerebrovascular accidents Stroke

Predominance of non-adiabatic effects in zero-point renormalization of the electronic band gap

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

npj Computational Materials 2020年第1期6卷 297-304页

作者： Anna Miglio Véronique Brousseau-Couture Emile Godbout Gabriel Antonius Yang-Hao Chan Steven G.Louie Michel Côté Matteo Giantomassi Xavier Gonze institute of Condensed Matter and Nanosciences UCLouvainB-1348 Louvain-la-NeuveBelgium Département de Physique et Regroupement Québécois sur les Matériaux de Pointe Universitéde MontrealC.P.6128Succursale Centre-VilleMontréal H3C 3J7Canada department of Physics University of California at BerkeleyBerkeleyCA 94720USA Materials Sciences Division Lawrence Berkeley National LaboratoryBerkeleyCA 94720USA Département de Chimie Biochimie et PhysiqueInstitut de recherche sur l’hydrogèneUniversitédu QuébecàTrois-RivièresTrois-Rivières G8Z 4M3Canada Skolkovo institute of Science and Technology Skolkovo Innovation CenterNobel St.3Moscow 143026Russia

Electronic and optical properties of materials are affected by atomic motion through the electron–phonon interaction:not only band gaps change with temperature,but even at absolute zero temperature,zero-point motion causes band-gap *** present a large-scale first-principles evaluation of the zero-point renormalization of band edges beyond the adiabatic *** materials with light elements,the band gap renormalization is often larger than 0.3 eV,and up to 0.7 *** effect cannot be ignored if accurate band gaps are *** infrared-active materials,global agreement with available experimental data is obtained only when non-adiabatic effects are taken into *** even dominate zero-point renormalization for many materials,as shown by a generalized Fröhlich model that includes multiple phonon branches,anisotropic and degenerate electronic extrema,whose range of validity is established by comparison with first-principles results.

关键词： adiabatic renormalization phonon

Modulation of the cutoff wavelength in the spectra for solar selective absorbing coating based on high-entropy films

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Minerals,Metallurgy and Materials 2020年第10期27卷 1371-1378页

作者： Ping Song Cong Wang Jie Ren Ying Sun Yong Zhang Angélique Bousquet Thierry Sauvage Eric Tomasella Center for Condensed Matter and Material Physics Beihang UniversityBeijing 100083China Key laboratory for Microstructural Material Physics of Hebei Province School of ScienceYanshan UniversityQinhuangdao 066004China Beijing Advanced Innovation Center of Materials Genome Engineering State Key Laboratory for Advanced Metals and MaterialsUniversity of Science and Technology BeijingBeijing 100083China universitéClermont Auvergne Centre national de la recherche scientifique(CNRS)SIGMA ClermontInstitut de Chimie de Clermont-Ferrand(ICCF)Clermont-Ferrand F-63000France Extreme Conditions and Materials:High Temperature and Irradiation(CEMHTI-uPR 3079 CNRS Orléans F-45100France

This paper demonstrates an intrinsic modulation of the cutoff wavelength in the spectra for solar selective absorbing coating based on high-entropy ***(NiCuCrFeSi)N((NCCFS)N)films were deposited by a magnetron sputtering *** backscattering spectroscopy analysis confirms the uniform composition and good homogeneity of these high-entropy *** real and imaginary parts of the permittivity for the(NCCFS)N material are calculated on the basis of the reflectance spectral fitting results.A redshift cutoff wavelength of the reflectance spectrum with increasing nitrogen gas flow rate exists because of the different levels of dispersion when changing nitrogen *** realize significant solar absorption,the film surface was reconstituted to match its impedance with air by designing a pyramid nanostructure *** with the absorptance of the as-deposited films,the designed metasurface obtains a significant improvement in solar absorption with the absorptance increasing from 0.74 to *** metasurfaces also show low mid-infrared emissions with thermal emittance that can be as low as *** results demonstrate a new idea in the design of solar selective absorbing surface with controllable absorptance and low infrared emission for high-efficiency photo-thermal conversion.

关键词： cutoff wavelength solar selective absorption infrared emission nanostructure high-entropy film

GABAB receptor activation protects neurons from apoptosis via IGF-I receptor transactivation

在线全文

学校读者我要写书评

暂无评论

GABAB receptor activation protects neurons from apoptosis vi...