检索结果-南通市图书馆

Mediator contributes to IgH locus VDJ rearrangements by promoting usage of most distal V segments

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2020年第4期17卷 407-409页

作者： Iman Dalloul Zeinab Dalloul Sandrine Le Noir François Boyer Bernardo Reina-San-Martin Jeanne Cook-Moreau Michel Cogné umr CNRS 7276 INSERM U1262Universite de LimogesLimoges 87025France inserm U1258 UMR CNRS 7104Institut de Genetique et de Biologie Moleculaire et CellulaireUniversitéde StrasbourgIllkirch 67400France

INTRODUCTION The immunoglobulin heavy(IgH)chain locus includes a large cluster of V,D and J segments spanning thousands of kilobases in mammals and its expression implies long-range 3D-regulation of various gene modifications.1 V(D)J recombination occurs during early B-cell ontogeny.2 It is regulated by multiple transcription factors and cis-regulatory elements,and most notably by the intronic 5′Eμelement located in the JH to Cμintron.3 The intergenic control region 1(IGCR1)located between the VH and D gene clusters inhibits rearrangement of the most DH-proximal VH gene segments,while promoting usage of distal VH gene segments.4 Other downstream elements of the locus,flanking its 3′end might have more subtle roles:while the large 3′regulatory region(3′RR)shows strong internal synergies2 and behaves as a superenhancer in mature B *** deletion showed no influence on the VDJ repertoire and rather increased transcription of unrearranged V segments.3–5 Insulators binding CTCF farther downstream of the 3′RR were shown,however,to promote usage of distal VH segments.6 Altogether,there are strong indications that elements involved in long-distance regulation of transcription might also influence VDJ ***-frame splicing of VDJ exons is also needed for full Ig expression.7 Given the functional importance of gene architecture and long-range interactions for the IgH locus physiology,we explored the dependence of the VDJ repertoire on Med1,a known actor of long-range enhancer interactions.

关键词： B cells mediator VDJ repertoire

Mettre en dialogue disciplines et personnes concernées par le cancer pour améliorer le parcours des soins en oncologie Dialogue between Disciplines and People Affected by Cancer to Improve the Path of Care in Oncology

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Psycho-Oncologie 2024年第2期18卷 95-100页

作者： Joëlle Kivits Frédérique Claudot Sandrine de Montgolfier Silvia Rossi UniversitéParis Cité ECEVEUMR 1123InsermParis75010France Universitéde Lorraine UMR 1319INSPIIREInsermNancy54500France Aix Marseille Univ InsermIRDSESSTIMChaire démocratie en santéet Engagement des personnes concernées par le CancerMarseille13284France

Interdisciplinarity and participatory research have become the guiding principles of cancer *** what are the practical forms and methods?Based on the experience of 3 successive research projects aimed at improving cancer care pathways-PARCA1,PARCA2 and 4P-we present the encounters between researchers on the one hand,and between these same researchers and patients and health professionals on the ***,these meetings have led to the co-construction of projects and the shared conduct of *** motivations behind the participatory approach are highlighted,as well as the‘step-by-step’approach needed to familiarize the people involved with the research process,and the limits of the approach:while everyone’s position tends to be adjusted as the projects progress,there is still an imbalance between the researchers and the people involved.

关键词： Interdisciplinarity transdisciplinarity participatory research healthcare pathways

Self-assembly of peptide-based nanostructures： Synthesis and biological activity

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nano Research 2018年第5期11卷 2315-2335页

作者： Lena Guyon Elise Lepeltier Catherine Passirani Micro & Nanomedecines Translationnelles-MINT UNIV Angers INSERM U 1066 CNRS UMR 6021 UBL Universite Bretagne Loire Angers F-49933 France

Peptide-based nanostructures have received much attention in the field of drug targeting. In fact, peptides have many advantages such as simplicity of the structure, biocompatibility, and chemical diversity. Moreover, some peptides, which are called cell-penetrating peptides, can cross cellular membranes and carry small molecules, small interfering RNA, or viruses inside live cells. These molecules are often covalently or noncovalently linked to cargoes, thus forming amphiphilic conjugates that can self-assemble. Supramolecular nanostructures formed from peptides are used in nanomedicine as a carrier to protect a drug and to target cancer cells. This review explores aliphatic-chain-conjugated peptides and drug-conjugated peptides that can self-assemble. Special emphasis is placed on the synthesis procedure, nanostructure formation, and biological activity.

关键词： self-assembly cell penetrating peptide nanoparticle peptide

Comparative effects of α2δ-1 ligands in mouse models of colonic hypersensitivity

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2016年第31期22卷 7111-7123页

作者： Mathieu Meleine Ludivine Boudieu Agathe Gelot Emilie Muller Amandine Lashermes Julien Matricon Celine Silberberg Vassilia Theodorou Alain Eschalier Denis Ardid Frederic A Carvalho Clermont Université Université d'Auvergne INSERM 1107 Neuro-Dol Université d’Auvergne INSERM 1107 Neuro-Dol INRA EI-Purpan UMR 1054 Neuro-Gastroenterology and Nutrition

AIM: To investigate anti-hypersensitive effects of α2δ-1 ligands in non-inflammatory and inflammationassociated colonic hypersensitivity(CHS) mouse ***: To induce an inflammation-associated CHS, 1% dextran sulfate sodium(DSS) was administered to C57Bl/6J male mice, in drinking water, for 14 d. Regarding the non-inflammatory neonatal maternal separation(NMS)-induced CHS model, wild-type C57BI/6J pups were isolated from their mother from day 2 to day 14(P2 to P14), three hours per day(from 9:00 a.m. to 12:00 p.m.). Colorectal distension was performed by inflating distension probe from 20 μL to 100 μL by 20 μL increment step every 10 s. After a first colorectal distension(CRD), drugs were administered subcutaneously, in a cumulative manner,(Gabapentin at 30 mg/kg and 100 mg/kg; Pregabalin at 10 mg/kg and 30 mg/kg; Carbamazepine at 10 mg/kg and 30 mg/kg) and a second CRD was performed one hour after each ***: The visceromotor response(VMR) to CRD was increased by our NMS paradigm protocol in comparison to non-handled(NH) mice, considering the highest distension volumes(80 μL: 0.783 ± 0.056 mV /s vs 0.531 ± 0.034 m V/s, P < 0.05 and 100 μL: 1.087 ± 0.056 m V/s vs 0.634 ± 0.038 m V/s, P < 0.05 for NMS and NH mice, respectively). In the inflammationassociated CHS, DSS-treated mice showed a dramatic and significant increase in VMR at 60 and 80 μL distension volumes when compared to control mice(60 μL: 0.920 ± 0.079 m V/s vs 0.426 ± 0.100 m V/s P < 0.05 and 80 μL: 1.193 ± 0.097 mV /s vs 0.681 ± 0.094 mV /s P < 0.05 for DSS- and Water-treated mice, respectively). Carbamazepine failed to significantly reduce CHS in both models. Gabapentin significantly reduced CHS in the DSS-induced model for both subcutaneous injections at 30 or 100 mg/kg. Pregabalin s i g n i f i c a n t l y r e d u c e d V M R t o C R D i n t h e n o n-inflammatory NMS-induced CHS model for the acute subcutaneous administration of the highest cumulative dose(30 mg/kg) and significan

关键词： Neonatal maternal separation Dextran sulfate sodium Colonic hypersensitivity mouse models Colorectal distension α2δ-1 ligands

Recent advances in cytokines:Therapeutic implications for inflammatory bowel diseases

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2011年第5期17卷 547-556页

作者： Guillaume Bouguen Jean-Baptiste Chevaux Laurent Peyrin-Biroulet inserm umr991 Department of HepatoGastroenterologyUniversity Hospital of RennesPontchaillou35000 RennesFrance inserm U954 Department of Hepato-GastroenterologyUniversity Hospital of Nancy54511 Vandoeuvre-les-NancyFrance

Inflammatory bowel diseases （IBDs） are complex and chronic disabling conditions resulting from a dysregulated dialogue between intestinal microbiota and components of both the innate and adaptive immune systems. Cytokines are essential mediators between activated immune and non-immune cells, including epithelial and mes- enchymal cells. They are immunomodulatory peptides released by numerous cells and these have significant effects on immune function leading to the differentiation and survival of T cells. The physiology of IBD is becom- ing a very attractive field of research for development of new therapeutic agents. These include cytokines involved in intestinal immune inflammation. This review will focus on mechanisms of action of oytokines involved in IBD and new therapeutic opportunities for these diseases.

关键词： Inflammatory bowel disease Ulcerative coli-tis Crohn's disease Cytokine Pathophysiology Biologi-cal therapy

The RNA binding proteins TIA1 and TIAL1 promote Mcl1 mRNA translation to protect germinal center responses from apoptosis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2023年第9期20卷 1063-1076页

作者： Ines COsma-Garcia Mailys Mouysset Dunja Capitan-Sobrino Yann Aubert Martin Turner Manuel D.Diaz-Muñoz Institute for Infectious and Inflammatory Diseases(INFINITy) Inserm UMR1291CNRS UMR5051University Paul SabatierCHU PurpanToulouse 31024France Immunology Program The Babraham InstituteCambridgeUK

Germinal centers(GCs)are essential for the establishment of long-lasting antibody *** B cells rely on post-transcriptional RNA mechanisms to translate activation-associated transcriptional programs into functional changes in the cell ***,the critical proteins driving these key mechanisms are still ***,we show that the RNA binding proteins TIA1 and TIAL1 are required for the generation of long-lasting GC ***1-and TIAL1-deficient GC B cells fail to undergo antigen-mediated positive selection,expansion and differentiation into B-cell clones producing high-affinity ***,TIA1 and TIAL1 control the transcriptional identity of dark-and light-zone GC B cells and enable timely expression of the prosurvival molecule ***,we demonstrate here that TIA1 and TIAL1 are key players in the post-transcriptional program that selects high-affinity antigen-specific GC B cells.

关键词： Adaptive immunity Germinal centers Post-transcriptional gene regulation RNA binding proteins Cell identity Apoptosis/10.1038/s41423-023-01063-4

Trans-silencing effect of the 3′RR immunoglobulin heavy chain enhancer on Igκtranscription at the pro-B cell stage

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2019年第7期16卷 668-670页

作者： Nour Ghazzaui Hussein Issaoui Ophélie Alyssa Martin Alexis Saintamand Jeanne Cook-Moreau Yves Denizot François Boyer CNRS umr 7276 Inserm U1262Universitéde LimogesLimogesFrance Present address:inserm U1236 UniversitéRennes 1RennesFrance

Due to their impact on nuclear organization,enhancers are master regulators of cell fate.1,2 The immunoglobulin heavy chain(IgH)locus undergoes numerous changes(such as transcription,accessibility,DNA breaks,and mutations)throughout B-cell *** of these events are controlled by the IgH 3′regulatory region(3′RR).The 3′RR is the master control element of mature B-cell IgH transcription,3 somatic hypermutation(SHM),4,5 conventional class switch recombination(CSR),4,6–10 and locus suicide recombination(LSR).11 In contrast,the 3′RR is expected to be dispensable for V(D)J recombination.12,13 During Bcell development,the heavy and light chain loci are poised for their VDJ and VJ rearrangements,*** IgH locus rearranges first,with D-J joining at the pro-B-cell stages,followed by V-DJ joining at the pre-B-cell *** Igk locus is poised for VJ rearrangements at the pre-B cell stage.

关键词： effect joining recombination

Neuroprotective and anti-inflammatory effects of a therapy combining agonists of nicotinic α7 and σ1 receptors in a rat model of Parkinson’s disease

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2021年第6期16卷 1099-1104页

作者： Steven Vetel Laura Foucault-Fruchard Claire Tronel Frédéric Buron Jackie Vergote Sylvie Bodard Sylvain Routier Sophie Sérrière Sylvie Chalon umr 1253 iBrainUniversitéde ToursInsermToursFrance CHU Tours Service pharmacieToursFrance Institut de Chimie Organique et Analytique ICOAUMR CNRS 7311Universitéd’OrléansOrléansFrance

To date there is no treatment able to stop or slow down the loss of dopaminergic neurons that characterizes Parkinson’s *** was recently observed in a rodent model of Alzheimer’s disease that the interaction between the α7 subtype of nicotinic acetylcholine receptor(α7-nAChR)and sigma-1 receptor(σ1-R)could exert neuroprotective effects through the modulation of neuroinflammation which is one of the key components of the pathophysiology of Parkinson’s *** this context,the aim of the present study was to assess the effects of the concomitant administration of N-(3R)-1-azabicyclo[2.2.2]oct-3-yl-furo[2,3-c]pyridine-5-carboxamide(PHA)543613 as an α7-nAChR agonist and 2-(4-morpholinethyl)1-phenylcyclohexanecarboxylate(PRE)-084 as aσ1-R agonist in a well-characterized 6-hydroxydopamine rat model of Parkinson’s *** animals received either vehicle separately or the dual therapy PHA/PRE once a day until day 14 *** no effect was noticed in the amphetamine-induced rotation test,our data has shown that the PHA/PRE treatment induced partial protection of the dopaminergic neurons(15-20%),assessed by the dopamine transporter density in the striatum and immunoreactive tyrosine hydroxylase in the substantia ***,this dual therapy reduced the degree of glial activation consecutive to the 6-hydroxydopamine lesion,i.e,the 18 kDa translocation protein density and glial fibrillary acidic protein staining in the striatum,and the CD11b and glial fibrillary acidic protein staining in the substantia ***,this study reports for the first time that concomitant activation of α7-nAChR andσ1-R can provide a partial recovery of the nigro-striatal dopaminergic neurons through the modulation of microglial *** study was approved by the Regional Ethics Committee(CEEA Val de Loire n°19)validated this protocol(Authorization N°00434.02)on May 15,2014.

关键词： 6-hydroxydopamine astrocytes microglial activation neurodegeneration neuroinflammation nicotinicα7 receptor Parkinson’s disease PHA 543613 PRE-084 sigma-1 receptor

IgH locus suicide recombination does not depend on NHEJ in contrast to CSR in B cells

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2019年第2期16卷 201-202页

作者： Hend Boutouil François Boyer Jeanne Cook-Moreau Michel Cogné Sophie Péron CRIBL UMR CNRS 7276 INSERM U126287000 LimogesFrance Institut Universitaire de France 75000 ParisFrance

IgH locus suicide recombination(LSR)occurs in activated Blymphocytes and may result in the death of the concerned B cell,justifying the name“locus suicide recombination”.1 LSR,as for immunoglobulin(Ig)class switch recombination(CSR),is an intrachromosomal recombination in the Ig heavy chain(IgH)***,LSR somehow appears to be the detrimental counterpart of CSR in activated B cells,since CSR intends for cells to actively participate in immune responses while LSR eliminates B ***,an important question to address is the regulation and the exact molecular mechanism of LSR.

关键词： CSR eliminate concerned