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Seasonal variations in dissolved organic matter concentration and composition in an outdoor system for bank filtration simulation

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Journal of Environmental Sciences 2024年第1期135卷 252-261页

作者： Muhammad Zeeshan Omamah Ali Shamas Tabraiz Aki Sebastian Ruhl German Environment Agency Section II 3.3Schichauweg 5812307BerlinGermany Technische universitat berlin Water TreatmentKF4Str.des 17.Juni 13510623BerlinGermany Natural and Applied Sciences Section School of Psychology and Life SciencesCanterbury Christ Church UniversityCanterburyCT11QUUK

Dissolved organic matter(DOM)in surface waters can vary markedly in character depending on seasonal variations such as rainfall intensity,UV radiations and *** in DOM as well as temperature and rainfall intensity over the year can affect the biochemical processes occurring in bank filtration(BF).Identification and characterization of DOM in the surface water could help to optimize the water treatment and provide stable and safe drinking *** study investigated year-long variations of DOM concentrations and compositions in a surface water of a circulated outdoor pond(research facility)connected to a BF *** was dominated by humic substances and a changing pattern of DOM in surface water was observed throughout the year.A significant increase of DOM(~38%)in surface water was noted in August compared to *** fluorescent DOM showed that DOM in summer was enriched with the degradable fraction whilst non-degradable fraction was dominated in winter.A constant(1.7±0.1 mg/L)effluent DOM was recirculated in the system throughout the *** removal through BF varied between 4%to 39%and was achieved within a few meters after infiltration and significantly correlated with influent DOM concentration(R^(2)=0.82,p0.05)change in the removal of DOM was observed in two subsurface layers(upper and lower).This study highlights the presence of a constant non-degradable DOM in the bank filtrate,which was not affected by temperature,redox conditions and UV radiations.

关键词： Bank filtration Dissolved organic matter Dissolved oxygen Fluorescence spectroscopy Excitation-emission matrix

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Orbit and clock products for quad-system satellites with undifferenced ambiguity fixing approach

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Satellite Navigation 2024年第1期5卷 125-139页

作者： Jiaqi Wu Xingxing Li Yongqiang Yuan Keke Zhang Xin Li Jiaqing Lou Yun Xiong School of Geodesy and Geomatics Wuhan University129 Luoyu RoadWuhan430079China Hubei Luojia Laboratory 129 Luoyu RoadWuhan430079China German Research Centre for Geosciences GFZ Telegrafenberg14473PotsdamGermany Technische universitat berlin(TUB) Straße Des 17.Juni10623BerlinGermany

Integer Ambiguity Resolution(IAR)can significantly improve the accuracy of GNSS Precise Orbit Determination(POD).Traditionally,the IAR in POD is achieved at the Double Differenced(DD)*** this contribution,we develop an Un-Differenced(UD)IAR method for Global Positioning System(GPS)+BeiDou Navigation Satellite System(BDS)+Galileo navigation satellite system(Galileo)+Global'naya Navigatsionnaya Sputnikovaya Sistema(GLONASS)quad-system POD by calibrating UD ambiguities in the raw carrier phase and generating the so-called carrier *** on this method,we generate the UD ambiguity-fixed orbit and clock products for the Wuhan Innovation Application Center(IAC)of the International GNSS Monitoring and Assessment System(iGMAS).One-year observations in 2020 from 150 stations are employed to investigate performance of orbit and clock ***,the UD Ambiguity Resolution(AR)yields more resolved integer ambiguities than the traditional DD AR,scaling up to 9%,attributable to its avoidance of station baseline *** from the removal of ambiguity parameters,the computational efficiency of parameter estimation undergoes a substantial 70%*** with the float solution,the orbit consistencies of UD AR solution achieve the accuracy of 1.9,5.2,2.8,2.1,and 2.7 cm for GPS,BeiDou-2 Navigation Satellite System(BDS-2),BeiDou-3 Navigation Satellite System(BDS-3),Galileo,and GLONASS satellites respectively,reflecting enhancements of 40%,24%,54%,34%,and 42%.Moreover,the standard deviations of Satellite Laser Ranging(SLR)residuals are spanning 2.5–3.5 cm,underscoring a comparable accuracy to the DD AR solution,with discrepancies below 5%.A notable advantage of UD AR lies in its capability to produce the Integer Recovered Clock(IRC),facilitating Precise Point Positioning(PPP)AR without requiring additional Uncalibrated Phase Delay(UPD)*** assess the performance of quad-system kinematic PPP based on IRC,a network comprising 120 stations is uti

关键词： Multi-GNSS Precise orbit determination Integer recover clock Undifferenced ambiguity resolution iGMAS innovation application center

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Deep learning-based detection of indicator species for monitoring biodiversity in semi-natural grasslands

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Environmental Science and Ecotechnology 2024年第5期21卷 238-247页

作者： Deepak H.Basavegowda Inga Schleip Paul Mosebach Cornelia Weltzien Technische universitat berlin Straße des 17.Juni 13510623BerlinGermany Leibniz-Institut für Agrartechnik und Biookonomie e.V.(ATB) Max-Eyth-Allee 10014469PotsdamGermany Hochschule für nachhaltige Entwicklung Eberswalde Schicklerstraße 516225EberswaldeGermany

Deep learning(DL)has huge potential to provide valuable insights into biodiversity changes in speciesrich agricultural ecosystems such as semi-natural grasslands,helping to prioritize and plan conservation ***,DL has been underexplored in grassland conservation efforts,hindered by data scarcity,intricate ecosystem interactions,and limited economic ***,we developed a DL-based object-detection model to identify indicator species,a group of vascular plant species that serve as surrogates for biodiversity assessment in high nature value(HNV)*** selected indicator species Armeria maritima,Campanula patula,Cirsium oleraceum,and Daucus *** overcome the hurdle of limited data,we grew indicator plants under controlled greenhouse conditions,generating a sufficient dataset for DL model *** model was initially trained on this greenhouse ***,smaller datasets derived from an experimental grassland plot and natural grasslands were added to the training to facilitate the transition from greenhouse to field *** optimized model achieved remarkable average precision(AP)on test datasets,with 98.6 AP50 on greenhouse data,98.2 AP50 on experimental grassland data,and 96.5 AP50 on semi-natural grassland *** findings highlight the innovative application of greenhouse-grown specimens for the in-situ identification of plants,bolstering biodiversity monitoring in grassland ***,the study illuminates the promising role of DL techniques in conservation programs,particularly as a monitoring tool to support result-based agrienvironment schemes.

关键词： Species-rich grasslands Biodiversity monitoring Object detection Cross-domain knowledge transfer

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Dissecting the molecular basis of spike traits by integrating gene regulatory networks and genetic variation in wheat

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Plant Communications 2024年第5期5卷 57-74页

作者： Guo Ai Chao He Siteng Bi Ziru Zhou Ankui Liu Xin Hu Yanyan Liu Liujie Jin JiaCheng Zhou Heping Zhang Dengxiang Du Hao Chen Xin Gong Sulaiman Saeed Handong Su Caixia Lan Wei Chen Qiang Li Hailiang Mao Lin Li Hao Liu Dijun Chen Kerstin Kaufmann Khaled FAlazab Wenhao Yan National Key Laboratory of Crop Genetic Improvement Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan 430070China College of Plant Science and Technology Huazhong Agricultural UniversityWuhan 430070China Shenzhen Institute of Nutrition and Health Huazhong Agricultural UniversityWuhan 430070China Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureGenomeAnalysis Laboratory of the Ministry of AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120China State Key Laboratory of Pharmaceutical Biotechnology School of Life SciencesNanjing UniversityNanjing 210023China Department for Plant Cell and Molecular Biology Institute for BiologyHumboldt-Universitat Zu Berlin10115 BerlinGermany Plant Research Department Nuclear Research CenterEgyptian Atomic Energy AuthorityCairo 13759Egypt

Spike architecture inﬂuences both grain weight and grain number per spike,which are the two major components of grain yield in bread wheat(Triticum aestivum L.).However,the complex wheat genome and the inﬂuence of various environmental factors pose challenges in mapping the causal genes that affect spike ***,we systematically identified genes involved in spike trait formation by integrating information on genomic variation and gene regulatory networks controlling young spike development in *** identified 170 loci that are responsible for variations in spike length,spikelet number per spike,and grain number per spike through genome-wide association study and meta-QTL *** constructed gene regulatory networks for young inﬂorescences at the double ridge stage and theﬂoret primordium stage,in which the spikelet meristem and theﬂoret meristem are predominant,respec-tively,by integrating transcriptome,histone modification,chromatin accessibility,eQTL,and protein–pro-tein interactome *** these networks,we identified 169 hub genes located in 76 of the 170 QTL regions whose polymorphisms are significantly associated with variation in spike *** functions of TaZF-B1,VRT-B2,and TaSPL15-A/D in establishment of wheat spike architecture were *** study provides valuable molecular resources for understanding spike traits and demonstrates that combining genetic analysis and developmental regulatory networks is a robust approach for dissection of complex traits.

关键词： bread wheat spike traits genetic variation protein–protein interaction gene regulatory network

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Promoting the production of challenging proteins via induced expression in CHO cells and modified cell-free lysates harboring T7 RNA polymerase and mutant eIF2α

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Synthetic and Systems Biotechnology 2024年第3期9卷 416-424页

作者： Jeffrey L.Schloßhauer Lena Tholen Alexander Korner Stefan Kubick Sofia Chatzopoulou Anja Honow Anne Zemella Fraunhofer Project Group PZ-Syn of the Fraunhofer Institute for Cell Therapy and Immunology-IZI Branch Bioanalytics and Bioprocesses-IZI-BBAm MühlenbergPotsdamGermany Fraunhofer Institute for Cell Therapy and Immunology-IZI Branch Bioanalytics and Bioprocesses-IZI-BBAm MühlenbergPotsdamGermany Faculty of Health Sciences Joint Faculty of the Brandenburg University of Technology Cottbus-SenftenbergThe Brandenburg Medical School Theodor FontaneUniversity of PotsdamPotsdamGermany Institute for Chemistry and Biochemistry Laboratory of Protein BiochemistryFreie Universitat BerlinThielallee 6314195BerlinGermany Institute of Biotechnology Technische Universit¨at BerlinStraße des 17.Juni 13510623BerlinGermany New/era/mabs GmbH August-Bebel-Str.8914482PotsdamGermany

Chinese hamster ovary(CHO)cells are crucial in biopharmaceutical production due to their scalability and capacity for human-like post-translational ***,toxic proteins and membrane proteins are often difficult-to-express in living ***,cell-free protein synthesis can be *** study explores innovative strategies for enhancing the production of challenging proteins through the modification of CHO cells by investigating both,cell-based and cell-free approaches.A major result in our study involves the integration of a mutant eIF2 translation initiation factor and T7 RNA polymerase into CHO cell lysates for cell-free protein *** resulted in elevated yields,while eliminating the necessity for exogenous additions during cell-free production,thereby substantially enhancing ***,we explore the potential of the Rosa26 genomic site for the integration of T7 RNA polymerase and cell-based tetracycline-controlled protein *** findings provide promising advancements in bioproduction technologies,offering flexibility to switch between cell-free and cell-based protein production as needed.

关键词： Inducible expression CHO cells Cell-free protein synthesis CRISPR T7 RNA polymerase eIF2 Rosa26

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Monte Carlo Simulations of Elastic Scattering with Applications to DC and High Power Pulsed Magnetron Sputtering for Ti3_(S)iC_(2)

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Communications in Computational Physics 2012年第5期11卷 1618-1642页

作者： Jurgen Geiser Sven Blankenburg Department of Mathematics Humboldt-Universitat zu BerlinD-10099 BerlinGermany Department of Physics Humboldt-Universitat zu BerlinD-10099 BerlinGermany.

We simulate the particle transport in a thin film deposition process made by PVD(physical vapor deposition)and present several models for projectile and target collisions in order to compute the mean free path and the differential cross section(angular distribution of scattered projectiles)of the scattering process.A detailed description of collision models is of the highest importance in Monte Carlo simulations of high power impulse magnetron sputtering and DC *** derive an equation for the mean free path for arbitrary interactions(cross sections)that includes the relative velocity between the *** apply our results to two major interaction models:hard sphere interaction&screened Coulomb *** types of interaction separate DC sputtering from HIPIMS.

关键词： DC sputtering MAX-phases mean free path particle in cell Monte Carlo collisions Monte Carlo Markov chain

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HOW TO PROVE THE DISCRETE RELIABILITY FOR NONCONFORMING FINITE ELEMENT METHODS

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Journal of Computational Mathematics 2020年第1期38卷 142-175页

作者： Carsten Carstensen Sophie Puttkammer Department of Mathematics Humboldt-Universitat zu BerlinUnter den Linden 610099 BerlinGermany

Optimal convergence rates of adaptive finite element methods are well understood in terms of the axioms of *** key ingredient is the discrete reliability of a residualbased a posteriori error estimator,which controls the error of two discrete finite element solutions based on two nested *** the error analysis of nonconforming finite element methods,like the Crouzeix-Raviart or Morley finite element schemes,the difference of the piecewise derivatives of discontinuous approximations to the distributional gradients of global Sobolev functions plays a dominant role and is the object of this *** nonconforming interpolation operator,which comes natural with the definition of the aforementioned nonconforming finite element in the sense of Ciarlet,allows for stability and approximation properties that enable direct proofs of the reliability for the residual that monitors the equilibrium *** novel approach of this paper is the suggestion of a right-inverse of this interpolation operator in conforming piecewise polynomials to design a nonconforming approximation of a given coarse-grid approximation on a refined *** results of this paper allow for simple proofs of the discrete reliability in any space dimension and multiply connected domains on general shape-regular triangulations beyond newest-vertex bisection of *** attention is on optimal constants in some standard discrete estimates listed in the appendices.

关键词： Discrete reliability Nonconforming finite element method Conforming companion Morley Crouzeix-Raviart Explicit constants Axioms of adaptivity

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Real-world evidence for improved outcomes with histamine antagonists and aspirin in 22,560 COVID-19 patients

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Signal Transduction and Targeted Therapy 2021年第8期6卷 2297-2299页

作者： Cameron Mura Saskia Preissner Susanne Nahles Max Heiland Philip E.Bourne Robert Preissner School of Data Science and Deportment of Biomedical Engineering University of VirginiaCharlottesvilleVAUSA Department Oral and Maxillofacial Surgery Charite-Universitatsmedizin Berlincorporate member of Freie Universitat BerlinHumboldt-Universitat zu Berlinand Berlin Institute of HealthBerlinGermany Institute of Physiology and Science-IT Charite-Universitatsmedizin Berlincorporate member of Freie Universitat BerlinHumboldt-Universitat zu Berlinand Berlin Institute of HealthBerlinGermany

Dear Editor,The COVID-19 pandemic has driven great interest in the therapeutic potential of repurposed drugs with well-established benefits and safety profiles(toxicity,bioavailability,etc.),many of which act via sign... 详细信息

关键词： drugs category COV

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A Numerical Investigation for a Model of the Solid-Gas Phase in a Crystal Growth Apparatus

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Communications in Computational Physics 2008年第4期3卷 913-934页

作者： Jurgen Geiser humboldt-universitat zu berlin Department of Mathematics Unter den Linden 6D-10099 BerlinGermany.

We present discretization and solver methods for a model of the solid-gas phase in a crystal growth *** model equations are coupled Eulerian and heat-transfer equations with flux boundary *** a more detailed discussion we consider simpler equations and present time-and space-decomposition methods as solver methods to decouple the multi-physics *** present the error analysis for the discretization and solver *** experiments are performed for the Eulerian and heat-transfer equation using decomposition *** present a real-life application of a crystal growth apparatus,based on underlying stationary heat *** we discuss further error analysis and application to a more complex model of crystal growth.

关键词： Decomposition methods finite-volume discretization error analysis heat equation crystal growth.

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TRANSFORMATIONS FOR THE PRIZE-COLLECTING STEINER TREE PROBLEM AND THE MAXIMUM-WEIGHT CONNECTED SUBGRAPH PROBLEM TO SAP

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Journal of Computational Mathematics 2018年第3期36卷 459-468页

作者： Daniel Rehfeldt Thorsten Koch Department of Mathematics Technische Universitat Berlin Germany Zuse Institute berlin and Technische universitat berlin Germany

Transformations of Steiner tree problem variants have been frequently discussed in the literature. Besides allowing to easily transfer complexity results, they constitute a central pillar of exact state-of-the-art solvers for well-known variants such as the Steiner tree problem in graphs. In this article transformations for both the prize-collecting Steiner tree problem and the maximum-weight connected subgraph problem to the Steiner arborescence problem are introduced for the first time. Furthermore, the considerable implications for practical solving approaches will be demonstrated, including the computation of strong upper and lower bounds.

关键词： Prize-collecting Steiner tree problem Maximum-weight connected subgraphproblem Graph transformations Dual-ascent heuristics.

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