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Ray Wu,as remembered by a former student

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Science China(Life Sciences) 2009年第2期52卷 108-110页

作者： Jack Szostak an Investigator of the howard Hughes Medical institute

Some 35 years ago, I had the good fortune to be ac-cepted into Ray Wu’s lab as a graduate student. Ray was already well known for his pioneering studies in DNA se-quencing, and his lab was quietly humming with the ex-citement of the new approaches being developed for DNA labeling, sequencing and synthesis. Looking back, I am somewhat surprised that Ray accepted me into his lab-I

关键词： Ray Wu,as remembered by a former student DNA

Transcriptome analysis of ankylosed primary molars with infraocclusion

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International Journal of Oral Science 2020年第1期12卷 68-76页

作者： Annie Tong Yuh-Lit Chow Katie Xu Rita Hardiman Paul Schneider Seong-Seng Tan Melbourne Dental School The University of MelbourneMelbourneAustralia florey institute of Neuroscience The University of MelbourneMelbourneAustralia

Primary molar ankylosis with infraocclusion can retard dental arch development and cause dental asymmetry. Despite its widespread prevalence, little is known about its molecular etiology and pathogenesis. To address this, RNA sequencing was used to generate transcriptomes of furcal bone from infraoccluded(n = 7) and non-infraoccluded(n = 9) primary second molars, all without succeeding biscuspids. Of the 18 529 expressed genes, 432(2.3%) genes were differentially expressed between the two groups(false discovery rate < 0.05). Hierarchical clustering and principal component analysis showed clear separation in gene expression between infraoccluded and non-infraoccluded samples. Pathway analyses indicated that molar ankylosis is associated with the expression of genes consistent with the cellular inflammatory response and epithelial cell turnover. Independent validation using six expressed genes by immunohistochemical analysis demonstrated that the corresponding proteins are strongly expressed in the developing molar tooth germ, in particular the dental follicle and inner enamel epithelium. The descendants of these structures include the periodontal ligament, cementum, bone and epithelial rests of Malassez;tissues that are central to the ankylotic *** therefore propose that ankylosis involves an increased inflammatory response associated with disruptions to the developmental remnants of the dental follicle and epithelial rests of Malassez.

关键词： occlusion infra molar

Powering brain power: GLUT1 and the era of structure based human transporter biology

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National Science Review 2015年第1期2卷 3-4页

作者： Douglas C.Rees Chemistry and Chemical Engineering Howard Hughes Medical InstituteCalifornia Institute of Technology

Every student of biochemistry quickly appreciates the central role of glycolysis in cellular *** is not usually addressed in an introductory course is how glucose gets inside a cell in the irst *** integral membrane p... 详细信息

关键词： GLUT1 and the era of structure based human transporter biology Powering brain power

Decoding the transcriptome and DNA methylome of human primordial germ cells

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Science China(Life Sciences) 2015年第7期58卷 729-730页

作者： HE Chuan Department of Chemistry and institute for Biophysical Dynamics The University of Chicago howard Hughes Medical institute

Primordial germ cells （PGCs） are the embryonic founder cells of the gametes--the oocytes and sperms that are vital for transmitting genetic information faithfully and efficiently from one generation to the next and for maintaining the continuation of a species [1]. It is therefore critical to understand the crucial epigenetic processes during the development of this most fascinating lineage. The develop- ment of PGCs in mouse as a model organism has been well understood, including their specification, migration, localization to genital ridges, global epigenetic reprogramming, and sex differentiation. However, the mechanistic understanding of gene expression pattern and epigenetic reprogramming in the human PGCs is still largely unknown due to the limited materials and suitable technology available for analysis. By using a single-cell RNA sequencing method and a modified whole-genome bisulfite sequencing technique for limited numbers of cells pioneered by Professor Fuchou Tang and others, Chinese scientists at Peking Uni- versity have analyzed the transcriptome of human PGCs at migrating and gonadal stages across 15 weeks at the single-cell level and single-base resolution in a recent Cell publication [2]. Their work provides the first comprehensive view of the complex relationship of DNA methylation and gene expression during the global epigenetic reprogramming process of human PGCs.

关键词：原始生殖细胞 DNA甲基化人类解码录音表观遗传测序技术全基因组

Computationally designed protein activation

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National Science Review 2019年第4期6卷 609-610页

作者： David Baker Department of Biochemistry Institute for Protein Design University of Washington howard Hughes Medical institute

Time-resolved tools for activating proteins of interest in living systems can allow monitoring of the dynamics of biological processes such as signal transduction. Various approaches have been developed to engineer both constitutive and inducible protein activation (e.g. genetic fusion of light switchable proteins).Active-site decaging is another strategy,but the limited number of genetically encoded unnatural amino acids and associ-

关键词： pro Rosetta Computationally designed protein activation

Protein synthesis inhibitors disrupt the induction of behavioral sensitization to a single morphine exposure and regulate Hsp70 expression in the mouse nucleus accumbens

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Protein synthesis inhibitors disrupt the induction of behavi...

An unexpected role for ergothioneine

中国药理学会第十次全国学术会议

作者： Andrew J.Lawrence howard florey institute and Centre for Neuroscience ParkvilleVictoria 3010 Australia

De novo protein synthesis has been implicated as necessary for long-lasting changes in neuronal *** sensitization to a single exposure to addictive drugs is a form of neuroplasticity,but little is known about the impo...

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National Science Review 2015年第4期2卷 382-383页

作者： Zedu Huang Wilfred A.van der Donk institute for Genomic Biology Department of Chemistry Howard Hughes Medical InstituteUniversity of Illinois at Urbana-Champaign

Ever since the appearance of atmospheric oxygen,organisms have needed ways to protect themselves from damage caused by oxidative ***(GSH,Fig.1A)is the dominant organothiol in eukarya to detoxify products formed from o... 详细信息

关键词： EGT MSH An unexpected role for ergothioneine

Rolipram,a selective phosphodiesterase-4 inhibitor,decreases ethanol seeking and consumption in alcohol-preferring Fawn Hooded rats

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Rolipram,a selective phosphodiesterase-4 inhibitor,decreases...

Regulation of sister chromatid cohesion during the mitotic cell cycle

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Andrew J.Lawrence howard florey institute and Centre for Neuroscience ParkvilleVictoria 3010Australia

＜正＞Alcohol dependence is a complex psychiatric disorder demanding development of novel pharmacotherapy. Since the cyclic AMP（cAMP） signaling cascade has been implicated in mediating the behavioral response to alcohol, it would be of interest to determine whether phosphodiesterase-4（PDE4）,an enzyme that selectively hydrolyzes intracellular cAMP,is involved in the regulation of alcohol ***,the selective PDE4 inhibitor rolipram was used to measure its anti-drinking properties in alcohol-preferring Fawn-Hooded（FH/Wjd） *** showed that acute administration of rolipram（0.05,0.1,and 0.2 mg/kg） selectively and dose-dependently reduced 5%ethanol, but not 10%sucrose,operant self-administration;it also decreased voluntary 5%ethanol intake,but not water consumption,for the initial 30 min in rats showing high stable preference for *** course assessment revealed the significant anti-drinking effect of rolipram（0.1,0.2 mg/kg） lasted for 5 h in both continuous- and intermittentaccess to 5%or 10%***,chronic rolipram treatment decreased 5%ethanol consumption and preference during the drug treatment *** effect lasted even after withdrawal of drug *** locomotor activity was inhibited by rolipram at 0.1 and 0.2 mg/kg,the effect was present only in the initial about 20 min,which did not seem to alter long-term ethanol *** data suggest that PDE4 may play a promising role in ethanol drinking and dependent *** may be a potential treatment target for further development of anti-drinking drugs.

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Science China(Life Sciences) 2015年第11期58卷 1089-1098页

作者： ZHENG Ge YU HongTao howard Hughes Medical institute Department of Pharmacology University of Texas Southwestern Medical Center

Orderly execution of two critical events during the cell cycle––DNA replication and chromosome segregation––ensures the stable transmission of genetic materials. The cohesin complex physically connects sister chromatids during DNA replication in a process termed sister chromatid cohesion. Timely establishment and dissolution of sister chromatid cohesion is a prerequisite for accurate chromosome segregation, and is tight regulated by the cell cycle machinery and cohesin-associated proteins. In this review, we discuss recent progress in the molecular understanding of sister chromatid cohesion during the mitotic cell cycle.

关键词： cell cycle mitosis sister chromatid cohesion cohesin cohesin loading cohesin release DNA replication cohesion estab-lishment